Resource data sheet
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(n1)oxStayGold(c4)/pRSET

Fluorescent protein oxStayGold for fusion to the N-terminus of target molecule

Catalog number RDB19608
Resource name (n1)oxStayGold(c4)/pRSET
Clone info. For fusion of oxStayGold to the N-terminus of a target molecule (X). c4 is the appendage that facilitates fusions to the C-terminus of oxStayGold. oxStayGold is a variant of StayGold with two mutations: C174I and C208I; oxStayGold is well expressed also in the cytosolic fraction as StayGold. The gene for oxStayGold in this construct has been resynthesized with mammalian-preferred codons. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pRSET (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Ampicillin
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
 
Sequence (full) RDB19608zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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MTA, for use for not-for-profit academic purpose [Word]
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Please obtain written approval of Dr. Atsushi Miyawaki.
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提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19608 (n1)oxStayGold(c4)/pRSET DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The (n1)oxStayGold(c4)/pRSET was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19608).

Reference section:

Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., Miyawaki, A., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19608_B2Eap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: 6xHis,T7 tag,Xpress tag,insert 5'
Sequence file: RDB19608_B2Eaa.seq check
>D08836A4_B2Ea_1_lacIpro-B_F_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGNNN CGNNTCNGTG NGCGAGGNNG CGGAAGAGCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
  121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
  181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
  241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
  301 TGGTGTCTAC AGGCGAGGAG CTGTTTACCG GCGTGGTGCC CTTCAAGTTC CAGCTGAAGG
  361 GCACCATCAA CGGCAAGAGC TTCACCGTGG AAGGCGAGGG CGAGGGCAAT AGCCACGAGG
  421 GCAGCCACAA AGGCAAGTAC GTGTGCACCA GCGGCAAACT GCCAATGTCT TGGGCCGCCC
  481 TGGGAACTAG CTTCGGCTAT GGCATGAAGT ACTACACCAA GTACCCCAGC GGCCTGAAGA
  541 ACTGGTTCCA CGAGGTGATG CCCGAGGGCT TCACCTACGA CAGACACATC CAGTACAAGG
  601 GCGACGGCAG CATCCACGCC AAGCACCAGC ACTTCATGAA GAACGGCACC TACCACAACA
  661 TCGTGGAGTT CACCGGCCAG GACTTCAAGG AGAACAGCCC CGTGCTGANC GGCGACATGA
  721 ACGTGAGCCT GCCCAACGAG GTGCAGCACA TCCCCAGANA TGACGGCGTG GAGTGCCCAG
  781 TGACCCTGCT GTACCCTCTG CTGANCGANA AGANCAAGTG CGTGGANGCC TACCAGAACA
  841 CCNTCATCNA GCCCCTGCAC NATCAGCCAG CCCCCGATGT GNCNTANCNC NNGNTCANAA
  901 AGCANNANNN CCAGANCNNN GNNNANACCG ANNANANANN NNNCATCNTC CNNANCGAGA
961 NCNNNNNNNN CNNNNNGNNN NNNNNNN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,insert 3'
Sequence file: RDB19608_B2Eab.seq check
>D08836A4_B2Ea_1_f1ori_R3_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN NGTTGGGNNN GGCGNNCGGT GCGGGCCTCT TCGCTATTAC
   61 GCCAGATCCG GATATAGTTC CTCCTTTCAG CAAAAAACCC CTCAAGACCC GTTTAGAGGC
  121 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG
  181 GGCTTTGTTA GCAGCCGGAT CAAGCTTCGA ATTCTTACAC GGCAGAAGCA GAAGGCTCGT
  241 GCCATGGCAG GTGGGCCTCC AGGGTCTCGC TCTGGATGAT GTGGTCTCTC TCCTCGGTGT
  301 CGTCCTTGCT CTGGGTGTAC TGCTTTCTGA TCCAGTGGTA TGGCACATCG GGGGCTGGCT
  361 GATTGTGCAG GGGCTTGATG ATGGTGTTCT GGTAGGCCTC CACGCACTTG CTCTTGTCGC
  421 TCAGCAGAGG GTACAGCAGG GTCACTGGGC ACTCCACGCC GTCATCTCTG GGGATGTGCT
  481 GCACCTCGTT GGGCAGGCTC ACGTTCATGT CGCCGGTCAG CACGGGGCTG TTCTCCTTGA
  541 AGTCCTGGCC GGTGAACTCC ACGATGTTGT GGTAGGTGCC GTTCTTCATG AAGTGCTGGT
  601 GCTTGGCGTG GATGCTGCCG TCGCCCTTGT ACTGGATGTG TCTGTCGTAG GTGAAGCCCT
  661 CGGGCATCAC CTCGTGGAAC CAGTTCTTCA GGCCGCTGGG GTACTTGGTG TAGTACTTCA
  721 TGCCATAGCC GAAGCTAGTT CCCAGGGCGG CCCAAGACAT TGGCAGTTTG CCGCTGGTGC
  781 ACACGTACTT GCCTTTGTGN CTGCCCTCGT GGCTATTGCC CTCGCCCTCG CCTTCCACGG
  841 TGAANCTCTT GNCGTTGANG GTGCCCTTCN NCTGGAACTT GAANGGCACC ACGCCGGTAA
  901 ACAGCTCCTC GCCNGTANAN ACCATCNGAT NCTTANCGNC NTCGNCGNAC NNATNCCGAN
  961 CNNTTNGCTG TCCNCCANNC NNGCTANCNN TACCNNNNNG ANGANGANGN NGANNNNCCN
1021 GNANNTGNAT NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]