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hmAG407/pRSETB

Plasmid clone of fluorescent protein monomeric Azami-Green (mAG).

Catalog number RDB15229
Resource name hmAG407/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Azami-Green (mAG) engineered to have an excitation peak at 407 nm.
Refer nucleotide sequences AB107915.1 and AB108447.1 as references.
Vector backbone Plasmid
Gene/insert name Galaxea fascicularis Azami-Green cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A. (2003) A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278: 34167-34171.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15229 hmAG407/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15229_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15229_A7H9a.seq
>D04498D5_2_A7H9_T7_20bp_A01_01_ABI08.ab1
    1 AAAAATTTCT CTAAAAATAA TTTTGTTTAA CTTTAAGAAG GAGGATATAC ATATGCGGGG
   61 TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG
  121 GGATCTGTAC GACGATGACG ATAAGGATCC CACCATGGTG AGCGTGATCA AGCCCGAGAT
  181 GAAGATCAAG CTGTGCATGA GGGGCACCGT GAACGGCCAC AACTTCGTGA TCGAGGGCGA
  241 GGGCAAGGGC AACCCCTACG AGGGCACCCA GATCCTGGAC CTGAACGTGA CCGAGGGCGC
  301 CCCCCTGCCC TTCGCCTACG ACATCCTGAC CACCGTGTTC CAGTACGGCA ACAGGGCCTT
  361 CACCAAGTAC CCCGCCGACA TCCAGGACTA CTTCAAGCAG ACCTTCCCCG AGGGCTACCA
  421 CTGGGAGAGG AGCATGACCT ACGAGGACCA GGGCATCTGC ACCGCCACCA GCAACATCAG
  481 CATGAGGGGC GACTGCTTCT TCTACGACAT CAGGTTCGAC GGCACCAACT TCCCCCCCAA
  541 CGGCCCCGTG ATGCAGAAGA AGACCCTGAA GTGGGAGCCC GACACCGAGA AGATGTACGT
  601 GGAGGACGGC GTGCTGAAGG GCGACGTGAA CATGAGGCTG CTGCTGGAGG GCGGCGGCCA
  661 CTACAGGTGC GACGTCAAGA CCACCTACAA GGCCAAGAAG GAGGTGAGGC TGCCCGACGC
  721 CCACAAGATC GACCACAGGA TCGAGATCCT GAAGCACGAC AAGGACTACA ACAAGGTGAA
  781 GCTGTACGAG AACGCCGTGG CCAGGTACTC CATGCTGCCC AGCCAGGCCA AGTGAGAATT
  841 CGAAGCTTGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG
  901 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
  961 TGAAAGGAGG AACTATATCC CGGATCTGGG CGTAATAGCG AAGAGGCCCG CACCGATCGG
 1021 CCCTTTCCCA ACAGTTGCGC AGGCCTGAAT GGGCGAAATG GGACGCGCCC CTGTAGCGGC
 1081 GCATTAAGCG CGGCGGGTGT GTGGGTTACG CGCAGCGTGA CCGGCTACAC TTGCAGCGCC
1141 TAGCGCCCGG CTCATTCGCT TTTCTTCCAT TCCCTTTTCC TCTCGCCCAC ACGGATC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

2019.07.30

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