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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmAG407/pRSETB (#RDB15229)

Plasmid clone of fluorescent protein monomeric Azami-Green (mAG).

Clone info. Plasmid clone of fluorescent protein monomeric Azami-Green (mAG) engineered to have an excitation peak at 407 nm.
Comment Refer nucleotide sequences AB107915.1 and AB108447.1 as references.
Vector backbone Plasmid
Gene/insert name Galaxea fascicularis Azami-Green cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., J. Biol. Chem. 278: 34167-34171 (2003).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., J. Biol. Chem. 278: 34167-34171 (2003).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15229 hmAG407/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hmAG407/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15229).

Reference section:

Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15229_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp ()
Sequence file: RDB15229_A7H9a.seq check
>D04498D5_2_A7H9_T7_20bp_A01_01_ABI08.ab1
    1 AAAAATTTCT CTAAAAATAA TTTTGTTTAA CTTTAAGAAG GAGGATATAC ATATGCGGGG
   61 TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG
  121 GGATCTGTAC GACGATGACG ATAAGGATCC CACCATGGTG AGCGTGATCA AGCCCGAGAT
  181 GAAGATCAAG CTGTGCATGA GGGGCACCGT GAACGGCCAC AACTTCGTGA TCGAGGGCGA
  241 GGGCAAGGGC AACCCCTACG AGGGCACCCA GATCCTGGAC CTGAACGTGA CCGAGGGCGC
  301 CCCCCTGCCC TTCGCCTACG ACATCCTGAC CACCGTGTTC CAGTACGGCA ACAGGGCCTT
  361 CACCAAGTAC CCCGCCGACA TCCAGGACTA CTTCAAGCAG ACCTTCCCCG AGGGCTACCA
  421 CTGGGAGAGG AGCATGACCT ACGAGGACCA GGGCATCTGC ACCGCCACCA GCAACATCAG
  481 CATGAGGGGC GACTGCTTCT TCTACGACAT CAGGTTCGAC GGCACCAACT TCCCCCCCAA
  541 CGGCCCCGTG ATGCAGAAGA AGACCCTGAA GTGGGAGCCC GACACCGAGA AGATGTACGT
  601 GGAGGACGGC GTGCTGAAGG GCGACGTGAA CATGAGGCTG CTGCTGGAGG GCGGCGGCCA
  661 CTACAGGTGC GACGTCAAGA CCACCTACAA GGCCAAGAAG GAGGTGAGGC TGCCCGACGC
  721 CCACAAGATC GACCACAGGA TCGAGATCCT GAAGCACGAC AAGGACTACA ACAAGGTGAA
  781 GCTGTACGAG AACGCCGTGG CCAGGTACTC CATGCTGCCC AGCCAGGCCA AGTGAGAATT
  841 CGAAGCTTGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG
  901 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
  961 TGAAAGGAGG AACTATATCC CGGATCTGGG CGTAATAGCG AAGAGGCCCG CACCGATCGG
 1021 CCCTTTCCCA ACAGTTGCGC AGGCCTGAAT GGGCGAAATG GGACGCGCCC CTGTAGCGGC
 1081 GCATTAAGCG CGGCGGGTGT GTGGGTTACG CGCAGCGTGA CCGGCTACAC TTGCAGCGCC
1141 TAGCGCCCGG CTCATTCGCT TTTCTTCCAT TCCCTTTTCC TCTCGCCCAC ACGGATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]