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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmAG407/pRSETB

Plasmid clone of fluorescent protein monomeric Azami-Green (mAG).

Catalog number RDB15229
Resource name hmAG407/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Azami-Green (mAG) engineered to have an excitation peak at 407 nm.
Comment Refer nucleotide sequences AB107915.1 and AB108447.1 as references.
Vector backbone Plasmid
Gene/insert name Galaxea fascicularis Azami-Green cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Galaxea fascicularis Azami-Green

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A. (2003) A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278: 34167-34171.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A. (2003) A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278: 34167-34171.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15229 hmAG407/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15229_A7H9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp
            Sequence file: RDB15229_A7H9a.seq check
            >D04498D5_2_A7H9_T7_20bp_A01_01_ABI08.ab1
                1 AAAAATTTCT CTAAAAATAA TTTTGTTTAA CTTTAAGAAG GAGGATATAC ATATGCGGGG
               61 TTCTCATCAT CATCATCATC ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG
              121 GGATCTGTAC GACGATGACG ATAAGGATCC CACCATGGTG AGCGTGATCA AGCCCGAGAT
              181 GAAGATCAAG CTGTGCATGA GGGGCACCGT GAACGGCCAC AACTTCGTGA TCGAGGGCGA
              241 GGGCAAGGGC AACCCCTACG AGGGCACCCA GATCCTGGAC CTGAACGTGA CCGAGGGCGC
              301 CCCCCTGCCC TTCGCCTACG ACATCCTGAC CACCGTGTTC CAGTACGGCA ACAGGGCCTT
              361 CACCAAGTAC CCCGCCGACA TCCAGGACTA CTTCAAGCAG ACCTTCCCCG AGGGCTACCA
              421 CTGGGAGAGG AGCATGACCT ACGAGGACCA GGGCATCTGC ACCGCCACCA GCAACATCAG
              481 CATGAGGGGC GACTGCTTCT TCTACGACAT CAGGTTCGAC GGCACCAACT TCCCCCCCAA
              541 CGGCCCCGTG ATGCAGAAGA AGACCCTGAA GTGGGAGCCC GACACCGAGA AGATGTACGT
              601 GGAGGACGGC GTGCTGAAGG GCGACGTGAA CATGAGGCTG CTGCTGGAGG GCGGCGGCCA
              661 CTACAGGTGC GACGTCAAGA CCACCTACAA GGCCAAGAAG GAGGTGAGGC TGCCCGACGC
              721 CCACAAGATC GACCACAGGA TCGAGATCCT GAAGCACGAC AAGGACTACA ACAAGGTGAA
              781 GCTGTACGAG AACGCCGTGG CCAGGTACTC CATGCTGCCC AGCCAGGCCA AGTGAGAATT
              841 CGAAGCTTGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG
              901 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
              961 TGAAAGGAGG AACTATATCC CGGATCTGGG CGTAATAGCG AAGAGGCCCG CACCGATCGG
             1021 CCCTTTCCCA ACAGTTGCGC AGGCCTGAAT GGGCGAAATG GGACGCGCCC CTGTAGCGGC
             1081 GCATTAAGCG CGGCGGGTGT GTGGGTTACG CGCAGCGTGA CCGGCTACAC TTGCAGCGCC
            1141 TAGCGCCCGG CTCATTCGCT TTTCTTCCAT TCCCTTTTCC TCTCGCCCAC ACGGATC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl