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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mKeima (h)/pCS2

Expression vector of monomeric Keima-Red (mKeima).

Catalog number RDB15236
Resource name mKeima (h)/pCS2
Clone info. Expression vector of monomeric Keima-Red (mKeima).
Vector backbone pCS2+ (Plasmid)
Selectable markers Amp^r.
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Montipora. sp RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15236 mKeima (h)/pCS2 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15236_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCALNLF1
            Region: SV40 pA, T7 pro, insert, SP6 pro, CMV IE94 pro
            Sequence file: RDB15236_A7H9a.seq check
            >D04498E3-1_A7H9_pAxCALNL_F1_A01_01_ABI08.ab1
                1 ATTTAAGGTT TTCCTTATCA TGTCTGGATC TACGTAATAC GACTCACTAT AGTTCTAGAG
               61 GCTCGAGAGG CCTTGAATTC TTAGCCCAGC AGGGAGTGGC GGGCGATGGC GATCTCGCAC
              121 TGCTCCACGC TGGTGTAGTC GCGGTTGTGG CTGGTCACGT CCAGCTTGCG GTCGATCTCG
              181 TGGCGGCCGG GCATCCTCAC GGGCTTCTTG GCCTTGTAGG TGCTCTTGAA CTCGCACAGG
              241 TAGTGGCCGC CGCCCTCCAG CTTCAGGGCC ATGTAGTCGT TGCCGATCAG CATTCCGTCG
              301 CGGGCGAACA GGCGCTCGGT GCTGGGCTCC CAGCCCTGGG TCTTCTTCTG CATCACGGGG
              361 CCGTTGGGGG GGAAGTTCTC GCCGCTGATC TTCACGTTGT AGATGAAGCA GTTGCCCTGG
              421 ATGCTGCTGT CGTTGCTCAC GGTGCACACG GCGCCGTCCT CGAAGTTCAT GCTGCGCTCC
              481 CAGGTGTAGC CCTCGGGGAA GCTCTGCTTG AAGTAGTCGG GGATGTCCTC GGGGTACTTG
              541 GTGAAGGGGA TGCTGCCGTA CTGGAGCTGG GGGCTCAGGA TGTCCCAGGC GAAGGGCAGG
              601 GGGCCACCCT TGGTCACGGT CAGCTTCACG GTCTGCTCGC CCTCGTAGGG CTTGCCCTTG
              661 CCGTCGCCCT CCACCTCGAA GTAGTGGCCG TTCACGGTGC CGCTCATGTA CACCTTGTAG
              721 GTCATCTGCT TGGCGATCAC GCTCACCATG GCGGATCCTG CAAAAAGAAC AAGTAGCTTG
              781 TATTCTATAG TGTCACCTAA ATCAAGCTTG CTCCGACGTC CCCAGGCAGA ATGGCGGTTC
              841 CCTAAACGAG CATTGCTTAT ATAGACCTCC CATTAGGCAC GCCTACCGCC CATTTACGTC
              901 AATGGAACGC CCATTTGCGT CATTGCCCCT CCCCATTGAC GTCAATGGGG ATGTACTTGG
              961 CAGCCATCGC GGGCCATTTA CCGCCATTGA CGTCAATGGG AGTACTGCCA ATGTACCCTG
             1021 GCGTACTTCC AATAGTAATG TACTTGCCCA AGGTTACTAT TATAGATATG ATGTACTGCC
             1081 AAGTGGCCAT TTACGTCATG ACGTCATAGG GGGCGTGAGA CGATATGAAT TGGTCAATGA
            1141 AGCATTCCAA TTGAACGTTC AATGGTGTGG TTGGTTCCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl