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mKeima (h)/pCS2

Expression vector of monomeric Keima-Red (mKeima).

Catalog number RDB15236
Resource name mKeima (h)/pCS2
Clone info. Expression vector of monomeric Keima-Red (mKeima).
Vector backbone pCS2+ (Plasmid)
Selectable markers Amp^r.
Gene/insert name Montipora. sp RFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15236 mKeima (h)/pCS2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15236_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNL_F1 RDB15236_A7H9a.seq
>D04498E3-1_A7H9_pAxCALNL_F1_A01_01_ABI08.ab1
    1 ATTTAAGGTT TTCCTTATCA TGTCTGGATC TACGTAATAC GACTCACTAT AGTTCTAGAG
   61 GCTCGAGAGG CCTTGAATTC TTAGCCCAGC AGGGAGTGGC GGGCGATGGC GATCTCGCAC
  121 TGCTCCACGC TGGTGTAGTC GCGGTTGTGG CTGGTCACGT CCAGCTTGCG GTCGATCTCG
  181 TGGCGGCCGG GCATCCTCAC GGGCTTCTTG GCCTTGTAGG TGCTCTTGAA CTCGCACAGG
  241 TAGTGGCCGC CGCCCTCCAG CTTCAGGGCC ATGTAGTCGT TGCCGATCAG CATTCCGTCG
  301 CGGGCGAACA GGCGCTCGGT GCTGGGCTCC CAGCCCTGGG TCTTCTTCTG CATCACGGGG
  361 CCGTTGGGGG GGAAGTTCTC GCCGCTGATC TTCACGTTGT AGATGAAGCA GTTGCCCTGG
  421 ATGCTGCTGT CGTTGCTCAC GGTGCACACG GCGCCGTCCT CGAAGTTCAT GCTGCGCTCC
  481 CAGGTGTAGC CCTCGGGGAA GCTCTGCTTG AAGTAGTCGG GGATGTCCTC GGGGTACTTG
  541 GTGAAGGGGA TGCTGCCGTA CTGGAGCTGG GGGCTCAGGA TGTCCCAGGC GAAGGGCAGG
  601 GGGCCACCCT TGGTCACGGT CAGCTTCACG GTCTGCTCGC CCTCGTAGGG CTTGCCCTTG
  661 CCGTCGCCCT CCACCTCGAA GTAGTGGCCG TTCACGGTGC CGCTCATGTA CACCTTGTAG
  721 GTCATCTGCT TGGCGATCAC GCTCACCATG GCGGATCCTG CAAAAAGAAC AAGTAGCTTG
  781 TATTCTATAG TGTCACCTAA ATCAAGCTTG CTCCGACGTC CCCAGGCAGA ATGGCGGTTC
  841 CCTAAACGAG CATTGCTTAT ATAGACCTCC CATTAGGCAC GCCTACCGCC CATTTACGTC
  901 AATGGAACGC CCATTTGCGT CATTGCCCCT CCCCATTGAC GTCAATGGGG ATGTACTTGG
  961 CAGCCATCGC GGGCCATTTA CCGCCATTGA CGTCAATGGG AGTACTGCCA ATGTACCCTG
 1021 GCGTACTTCC AATAGTAATG TACTTGCCCA AGGTTACTAT TATAGATATG ATGTACTGCC
 1081 AAGTGGCCAT TTACGTCATG ACGTCATAGG GGGCGTGAGA CGATATGAAT TGGTCAATGA
1141 AGCATTCCAA TTGAACGTTC AATGGTGTGG TTGGTTCCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

2019.02.26

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