Sequencing and PCR primers for primer information.
Expression vector of monomeric Keima-Red (mKeima).
| Catalog number | RDB15236 |
|---|---|
| Resource name | mKeima (h)/pCS2 |
| Clone info. | Expression vector of monomeric Keima-Red (mKeima). |
| Vector backbone | pCS2+ (Plasmid) |
| Selectable markers | Amp^r. |
| Gene/insert name | Montipora. sp RFP cDNA |
| Depositor | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
|---|---|
| Additional terms and conditions for distribution | The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word] |
| Remarks | Please obtain written approval of the depositor. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15236 | mKeima (h)/pCS2 | DNA solution |
Electronic file
Featured content
| Featured content | Dr. Miyawaki's resources (English text) |
|---|---|
| Featured content | Fluorescent protein resources (English text) |
| Featured content | Fluorescent protein resources (Japanese text) |
References
| original | Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15236_A7H9p1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCALNL_F1 | RDB15236_A7H9a.seq |
|---|---|
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>D04498E3-1_A7H9_pAxCALNL_F1_A01_01_ABI08.ab1 1 ATTTAAGGTT TTCCTTATCA TGTCTGGATC TACGTAATAC GACTCACTAT AGTTCTAGAG 61 GCTCGAGAGG CCTTGAATTC TTAGCCCAGC AGGGAGTGGC GGGCGATGGC GATCTCGCAC 121 TGCTCCACGC TGGTGTAGTC GCGGTTGTGG CTGGTCACGT CCAGCTTGCG GTCGATCTCG 181 TGGCGGCCGG GCATCCTCAC GGGCTTCTTG GCCTTGTAGG TGCTCTTGAA CTCGCACAGG 241 TAGTGGCCGC CGCCCTCCAG CTTCAGGGCC ATGTAGTCGT TGCCGATCAG CATTCCGTCG 301 CGGGCGAACA GGCGCTCGGT GCTGGGCTCC CAGCCCTGGG TCTTCTTCTG CATCACGGGG 361 CCGTTGGGGG GGAAGTTCTC GCCGCTGATC TTCACGTTGT AGATGAAGCA GTTGCCCTGG 421 ATGCTGCTGT CGTTGCTCAC GGTGCACACG GCGCCGTCCT CGAAGTTCAT GCTGCGCTCC 481 CAGGTGTAGC CCTCGGGGAA GCTCTGCTTG AAGTAGTCGG GGATGTCCTC GGGGTACTTG 541 GTGAAGGGGA TGCTGCCGTA CTGGAGCTGG GGGCTCAGGA TGTCCCAGGC GAAGGGCAGG 601 GGGCCACCCT TGGTCACGGT CAGCTTCACG GTCTGCTCGC CCTCGTAGGG CTTGCCCTTG 661 CCGTCGCCCT CCACCTCGAA GTAGTGGCCG TTCACGGTGC CGCTCATGTA CACCTTGTAG 721 GTCATCTGCT TGGCGATCAC GCTCACCATG GCGGATCCTG CAAAAAGAAC AAGTAGCTTG 781 TATTCTATAG TGTCACCTAA ATCAAGCTTG CTCCGACGTC CCCAGGCAGA ATGGCGGTTC 841 CCTAAACGAG CATTGCTTAT ATAGACCTCC CATTAGGCAC GCCTACCGCC CATTTACGTC 901 AATGGAACGC CCATTTGCGT CATTGCCCCT CCCCATTGAC GTCAATGGGG ATGTACTTGG 961 CAGCCATCGC GGGCCATTTA CCGCCATTGA CGTCAATGGG AGTACTGCCA ATGTACCCTG 1021 GCGTACTTCC AATAGTAATG TACTTGCCCA AGGTTACTAT TATAGATATG ATGTACTGCC 1081 AAGTGGCCAT TTACGTCATG ACGTCATAGG GGGCGTGAGA CGATATGAAT TGGTCAATGA 1141 AGCATTCCAA TTGAACGTTC AATGGTGTGG TTGGTTCCA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. |
|---|
2019.02.26
GNP_filter3_RDBDEP_html_190131.pl