RDB15275 - mKO2-zCdt1(1/190) / pT2KXIGdeltain

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icon below as well as clone information before placing your order.

mKO2-zCdt1(1/190) / pT2KXIGdeltain (#RDB15275)

zCdt1-based G1 marker for zebrafish cells to label nuclei with orange fluorescent protein mKO2. A component of the zFucci.

Clone info.	zCdt1-based G1 marker for zebrafish cells to label nuclei with orange fluorescent protein mKO2. A component of the zFucci.
Vector backbone	pT2KXIGdeltain (plasmid)
Size of vector backbone	8.1 kb
Selectable markers	Amp^r
Gene/insert name	zebrafish zCdt1 cDNA Fungia concinna stony coral orange fluorescent protein cDNA
Reference of insert sequence	AB505859 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15275	mKO2-zCdt1(1/190) / pT2KXIGdeltain	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mKO2-zCdt1(1/190) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15275).

Reference section:

Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15275_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F () Sequence file: RDB15275_A7H9a.seq
>D004498I6_A7H9_1_beta-gi_F_H01_08_ABI08.ab1 1 CACAGGGGTC CAGGGCCTTC TTCTCTTTCC TACAGCTCCT GGGCAACGTG CTGGTTGTTG 61 TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGACGGATC CACTAGTAAC GGCCGCCAGT 121 GTGCTGGAAT TCGCCACCAT GGTGAGTGTG ATTAAACCAG AGATGAAGAT GAGGTACTAC 181 ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATTGAAG GTGAAGGCAC AGGCAGACCT 241 TACGAGGGAC ATCAAGAGAT GACACTACGC GTCACAATGG CCGAGGGCGG GCCAATGCCT 301 TTCGCGTTTG ACTTAGTGTC ACACGTGTTC TGTTACGGCC ACAGAGTATT TACTAAATAT 361 CCAGAAGAGA TACCAGACTA TTTCAAACAA GCATTTCCTG AAGGCCTGTC ATGGGAAAGG 421 TCGTTGGAGT TCGAAGATGG TGGGTCCGCT TCAGTCAGTG CGCATATAAG CCTTAGAGGA 481 AACACCTTCT ACCACAAATC CAAATTTACT GGGGTTAACT TTCCTGCCGA TGGTCCTATC 541 ATGCAAAACC AAAGTGTTGA TTGGGAGCCA TCAACCGAGA AAATTACTGC CAGCGACGGA 601 GTTCTGAAGG GTGATGTTAC GATGTACCTA AAACTTGAAG GAGGCGGCAA TCACAAATGC 661 CAAATGAAGA CTACTTACAA GGCGGCAAAA GAGATTCTTG AAATGCCAGG AGACCATTAC 721 ATCGGCCATC GCCTCGTCAG GAAAACCGAA GGCAACATTA CTGAGCAGGT AGAAGATGCA 781 GTAGCTCATT ACTCAATGTT GCCTTCCCAG GGCGGATATC CATCACACTG GCGGCCGCTC 841 GAGATGGCTC AAAGCTCGTG TTACTGATTA TTTTGCACAG AGTAAAAAAG CTGGTGTTTT 901 CTCGATCCTT TAACGGTCTA AGGGACAAAA GGTGTCCGGG GGATGTAGTA GAATCAGCCG 961 GTGATCAAAT AAACCCGAGG AGTTCGAGGC AGAGCGTCCC GGTTCCTCCA CGCAAAGCGT 1021 ACGCACAGTG AGATCACCAC AGCAGAAGCC GTCAGAAACA AACTCAGCTT GGAAGTTTTC 1081 TCCAAGTCAT CGAACGAGGG CTTTTATTCC ACCGGAAAAC CTGCAGAATA CCGCTCGGCG 1141 GGAATTCCGC GGTTGAAGTG AAAGATGGGA TGGACTTAAC GGGCTGAAAG TTCCCAGGAC 1201 ACCCCTCCTA AGCTCGGGAG AGTATTCTCT ACGCGGGATG ATGAT //
Primer: pAxCALNL_F1 () Sequence file: RDB15275_A7H9b.seq
>D04498I6_A7H9_1_pAxCALNL_F1_F05_17_ABI24.ab1 1 CTAAGGTATC TTATCATGTC TGGATCATCA TCGATTACTC TTTGCGGGCA GTTTGTTGAG 61 GTGCCTGAGG GCTGCTGCTG GCCAAGGGCT CAGTGGTCTT TACTTTGTCG TCGCTTGCCA 121 GTAGATAGAG TTTTTTCCTC GCAGTTTTCT GTCCAGCGCG CTCCTCCGGT GAGCTCCTGC 181 AGTTGTGGCC CGCGTTGAGT CTCTGCCGCT TCTTGGCGCT GCTATGCAGC TCGGCTGTGG 241 ACGGAAACAA CACCGAACAC ACGTCGAACT CCGGTGAACT CCGCTTAGGG GTCCTGGGAC 301 TCGCCGTTAA GCCCTCAATC TTCACATCAC GCGAATCCGC GACGGTATCT GCAGTCTCCG 361 TCGATAAAGC CTCGTCGATG ACTTTGAGAA ACTCCAGCTG AGTTTGTTTC TGCGGCTCTG 421 CTGTGGTGAT CTCACTGTGC GTCGCTTTGC GTGAAGAACC GGACGCTCTG CTCGAACTCC 481 TCGGTTTATT GATCACCGCT GATTCTACTA CATCCCCGGA CACCTTTTGT CCCTTAGACC 541 GTAAAGATCG AGAAACACCA GCTTTTTTAC TCTGTGCAAA ATAATCAGTA ACACGAGCTT 601 GAGCCATCTC GAGCGGCCGC CAGTGTGATG GATATCCGCC CTGGGAAGGC AACATTGAGT 661 AATGAGCTAC TGCATCTTCT ACCTGCTCAG TAATGTTGCC TTCGGTTTTC CTGACGAGGC 721 GATGGCCGAT GTAATGGTCT CCTGGCATTT CAAGAATCTC TTTTGCCGCC TTGTAAGTAG 781 TCTTCATTTG GCATTTGTGA TTGCCGCCTC CTTCAAGTTT TAGGTACATC GTAACATCAC 841 CCTTCAGAAC TCCGTCGCTG GCAGTAATTT TCTCGGTTGA TGGCTCCCAA TCAACACTTT 901 GGTTTTGCAT GATAGGACCA TCGGCAGGAA AGTTAACCCC CAGTAAATTT GGATTTGTGG 961 TAGAAGGTGT TTCCCTCTAA GCTTATATGC GCACTGACTG AAGCGGACCC ACCATCTTCG 1021 AACTCCACGA CCTTTCCCAT GACAGGCCTC AAGAAATGCC TGCTTGGAAT AGTCTGCAAT 1081 CTCTTCCTGC ATATTTTAGT AAATACCTCC CTG //
Primer: EF1a-N_R2 () Sequence file: RDB15275_A7H9c.seq
>D04498I6_A7H9_1_EF1a-N_R2_G05_20_ABI24.ab1 1 TTGCGGGCAA GATTGCAAGT TTGATTTGCA TTGGAAGGGT CGCTGGCTTT TGTGTTACAC 61 GCCCCTTATT TGTGCTTGAT TAGATGATCC CCCTGCTCGA GGGCCCATCT GGCCTGTGTT 121 TCAGACACCA GGGAGTCTCT GCTCACGTTT CCTGCTATTT GCAGCCTCTC TATCAAGACT 181 AATACACCTC TTCCCGCATC GGCTGCCTGT GAGAGGCTTT TCAGCACTGC AGGATTGCTT 241 TTCAGCCCCA AAAGAGCTAG GCTTGACACT AACAATTTTG AGAATCAGCT TCTACTGAAG 301 TTAAATCTGA GGTTTTACAA CTTTGAGTAG CGTGTACTGG CATTAGATTG TCTGTCTTAT 361 AGTTTGATAA TTAAATACAA ACAGTTCTAA AGCAGGATAA AACCTTGTAT GCATTTCATT 421 TAATGTTTTT TGAGATTAAA AGCTTAAACA AGAATCTCTA GTTTTCTTTC TTGCTTTTAC 481 TTTTACTTCC TTAATACTCA AGTACAATTT TAATGGAGTA CTTTTTTACT TTTACTCAAG 541 TAAGATTTCT AGCCCAGAAT AACTTTTTAC CTTTTTAAAA TTTGAGGGAT AAAAAATTTT 601 TTTCCCTCTA AGAAAAACTC TGTGTTGAAC ATCTCTCTCA CCTCTGTGGA GAAAAAAAAA 661 ATTTTTTGTG AGAGAAAACT CTTTTTTTTT ACCCCCCCCT GTCGGGAAAA AAAAAAATTC 721 CTCGTGGGAG AAAAAAAACC CCCCCGAAAC CCGGGGGGGG GGGGCCCCCA AGGGGGGGAA 781 AGGGGGGGGG GGGCGCCAAC AAAAAAATTT TACCCCCCCC CTTTTTTTTG GGAAAAAAAG 841 GGAAGGGGGC CCCTCTCGGG GGGGGGGGGG TGAGAAAAAA AAAAAACGCG GGGGGGCCAC 901 ACAAAGGGGG GGGGGGGGGG GACTCCCCTC TATCTTCAAA AAGATTTTTT TTTTTTTTTG 961 TGACAGAACA GAGAGAGAAC TCTCTCTTCT CTGTCATAAA AAAAGAAGAG AGGGGGGGGC 1021 GCCCCCCTCT GCTATATATA CCACCACTTT TTGGTGTGTC GCAGCACCAA TAGTGTGGGT 1081 CGTGCTTAGT CACTAGCCGG GCGCGTAAGA ATAGGATACG TCGACGAAGC CACAGAAGAT 1141 AATTATGATC CTC //
Primer: pAxEF_R2 () Sequence file: RDB15275_A7H9d.seq
>D004498I6_A7H9_1_pAxEF_R2_G01_07_ABI08.ab1 1 CCCCGGTAAA AATGCTTTAT TTGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA 61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG 121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT 181 ATGATCCTCT AGATCAGATC TGCGAAGATA CGGCCACGGG TGCTCTTGAT CCTGTGGCTG 241 ATTTTGGACT GTGCTGCTCG CAGCTGCTGA TGAATCACAT ACTTCCTCCA TTTTCTTCCA 301 CTGATTGACT GTTATAATTT CCCTAATTTC CAGGTCAAGG TGCTGTGCAT TGTGGTAATA 361 GATGTGACAT GACGTCACTT CCAAAGGACC AATGAACATG TCTGACCAAT TTCATATAAT 421 GTGAAAACGA TTTTCATAGG CAGAATAAAT AACATTTAAA TTAAACTGGG CATCAGCGCA 481 ATTCAATTGG TTTGGTAATA GCAAGGGAAA ATAGAATGAA GTGATCTCCA AAAAATAAGT 541 ACTTTTTGAC TGTAAATAAA ATTGTAAGGA GTAAAAAGTA CTTTTTTTTC TAAAAAAATG 601 TAATTAAGTA AAAGTAAAAG TATTGATTTT TAATTGTACT CAAGTAAAGT AAAAATCCCC 661 AAAAATAATA CTTAAGTACA GTAATCAAGT AAAATTACTC AAGTACTTTA CACCTCTGGT 721 TCTTGACCCC CTACCTTCAG CAAGCCCAGC AGATCCACTA GTTCTAGAGC GGCCGCCACC 781 GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC 841 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 901 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 961 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 1021 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTTCTCGCT 1081 CACTGAACTC GCTGCGCTCG GTCGTTTCGG GCTGGCCGCA GCGTATCAGC TCACCTCAAG 1141 GGCGGTAATA CCGGGTTATC TCCACTCACA GGATATCTAG GGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

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Other resources in our bank

zebrafish zCdt1 and ortholog

External Database
zebrafish zCdt1

Fungia concinna stony coral orange fluorescent protein

Reference sequence