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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mKO2-zCdt1(1/190) / pT2KXIGdeltain

zCdt1-based G1 marker for zebrafish cells to label nuclei with orange fluorescent protein mKO2. A component of the zFucci.

Catalog number RDB15275
Resource name mKO2-zCdt1(1/190) / pT2KXIGdeltain
Clone info. zCdt1-based G1 marker for zebrafish cells to label nuclei with orange fluorescent protein mKO2. A component of the zFucci.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name zebrafish zCdt1 cDNA Fungia concinna stony coral orange fluorescent protein cDNA
Reference of nucleotide sequence AB505859 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank zebrafish cdt1 (KEGG orthology K10727) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB15275 mKO2-zCdt1(1/190) / pT2KXIGdeltain DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Transposon and plasmid, Kawakami laboratory (English text)
Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15275_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F
Sequence file: RDB15275_A7H9a.seq check
>D004498I6_A7H9_1_beta-gi_F_H01_08_ABI08.ab1
    1 CACAGGGGTC CAGGGCCTTC TTCTCTTTCC TACAGCTCCT GGGCAACGTG CTGGTTGTTG
   61 TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGACGGATC CACTAGTAAC GGCCGCCAGT
  121 GTGCTGGAAT TCGCCACCAT GGTGAGTGTG ATTAAACCAG AGATGAAGAT GAGGTACTAC
  181 ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATTGAAG GTGAAGGCAC AGGCAGACCT
  241 TACGAGGGAC ATCAAGAGAT GACACTACGC GTCACAATGG CCGAGGGCGG GCCAATGCCT
  301 TTCGCGTTTG ACTTAGTGTC ACACGTGTTC TGTTACGGCC ACAGAGTATT TACTAAATAT
  361 CCAGAAGAGA TACCAGACTA TTTCAAACAA GCATTTCCTG AAGGCCTGTC ATGGGAAAGG
  421 TCGTTGGAGT TCGAAGATGG TGGGTCCGCT TCAGTCAGTG CGCATATAAG CCTTAGAGGA
  481 AACACCTTCT ACCACAAATC CAAATTTACT GGGGTTAACT TTCCTGCCGA TGGTCCTATC
  541 ATGCAAAACC AAAGTGTTGA TTGGGAGCCA TCAACCGAGA AAATTACTGC CAGCGACGGA
  601 GTTCTGAAGG GTGATGTTAC GATGTACCTA AAACTTGAAG GAGGCGGCAA TCACAAATGC
  661 CAAATGAAGA CTACTTACAA GGCGGCAAAA GAGATTCTTG AAATGCCAGG AGACCATTAC
  721 ATCGGCCATC GCCTCGTCAG GAAAACCGAA GGCAACATTA CTGAGCAGGT AGAAGATGCA
  781 GTAGCTCATT ACTCAATGTT GCCTTCCCAG GGCGGATATC CATCACACTG GCGGCCGCTC
  841 GAGATGGCTC AAAGCTCGTG TTACTGATTA TTTTGCACAG AGTAAAAAAG CTGGTGTTTT
  901 CTCGATCCTT TAACGGTCTA AGGGACAAAA GGTGTCCGGG GGATGTAGTA GAATCAGCCG
  961 GTGATCAAAT AAACCCGAGG AGTTCGAGGC AGAGCGTCCC GGTTCCTCCA CGCAAAGCGT
 1021 ACGCACAGTG AGATCACCAC AGCAGAAGCC GTCAGAAACA AACTCAGCTT GGAAGTTTTC
 1081 TCCAAGTCAT CGAACGAGGG CTTTTATTCC ACCGGAAAAC CTGCAGAATA CCGCTCGGCG
 1141 GGAATTCCGC GGTTGAAGTG AAAGATGGGA TGGACTTAAC GGGCTGAAAG TTCCCAGGAC
1201 ACCCCTCCTA AGCTCGGGAG AGTATTCTCT ACGCGGGATG ATGAT
//
Primer: pAxCALNL_F1
Sequence file: RDB15275_A7H9b.seq check
>D04498I6_A7H9_1_pAxCALNL_F1_F05_17_ABI24.ab1
    1 CTAAGGTATC TTATCATGTC TGGATCATCA TCGATTACTC TTTGCGGGCA GTTTGTTGAG
   61 GTGCCTGAGG GCTGCTGCTG GCCAAGGGCT CAGTGGTCTT TACTTTGTCG TCGCTTGCCA
  121 GTAGATAGAG TTTTTTCCTC GCAGTTTTCT GTCCAGCGCG CTCCTCCGGT GAGCTCCTGC
  181 AGTTGTGGCC CGCGTTGAGT CTCTGCCGCT TCTTGGCGCT GCTATGCAGC TCGGCTGTGG
  241 ACGGAAACAA CACCGAACAC ACGTCGAACT CCGGTGAACT CCGCTTAGGG GTCCTGGGAC
  301 TCGCCGTTAA GCCCTCAATC TTCACATCAC GCGAATCCGC GACGGTATCT GCAGTCTCCG
  361 TCGATAAAGC CTCGTCGATG ACTTTGAGAA ACTCCAGCTG AGTTTGTTTC TGCGGCTCTG
  421 CTGTGGTGAT CTCACTGTGC GTCGCTTTGC GTGAAGAACC GGACGCTCTG CTCGAACTCC
  481 TCGGTTTATT GATCACCGCT GATTCTACTA CATCCCCGGA CACCTTTTGT CCCTTAGACC
  541 GTAAAGATCG AGAAACACCA GCTTTTTTAC TCTGTGCAAA ATAATCAGTA ACACGAGCTT
  601 GAGCCATCTC GAGCGGCCGC CAGTGTGATG GATATCCGCC CTGGGAAGGC AACATTGAGT
  661 AATGAGCTAC TGCATCTTCT ACCTGCTCAG TAATGTTGCC TTCGGTTTTC CTGACGAGGC
  721 GATGGCCGAT GTAATGGTCT CCTGGCATTT CAAGAATCTC TTTTGCCGCC TTGTAAGTAG
  781 TCTTCATTTG GCATTTGTGA TTGCCGCCTC CTTCAAGTTT TAGGTACATC GTAACATCAC
  841 CCTTCAGAAC TCCGTCGCTG GCAGTAATTT TCTCGGTTGA TGGCTCCCAA TCAACACTTT
  901 GGTTTTGCAT GATAGGACCA TCGGCAGGAA AGTTAACCCC CAGTAAATTT GGATTTGTGG
  961 TAGAAGGTGT TTCCCTCTAA GCTTATATGC GCACTGACTG AAGCGGACCC ACCATCTTCG
 1021 AACTCCACGA CCTTTCCCAT GACAGGCCTC AAGAAATGCC TGCTTGGAAT AGTCTGCAAT
1081 CTCTTCCTGC ATATTTTAGT AAATACCTCC CTG
//
Primer: EF1a-N_R2
Sequence file: RDB15275_A7H9c.seq check
>D04498I6_A7H9_1_EF1a-N_R2_G05_20_ABI24.ab1
    1 TTGCGGGCAA GATTGCAAGT TTGATTTGCA TTGGAAGGGT CGCTGGCTTT TGTGTTACAC
   61 GCCCCTTATT TGTGCTTGAT TAGATGATCC CCCTGCTCGA GGGCCCATCT GGCCTGTGTT
  121 TCAGACACCA GGGAGTCTCT GCTCACGTTT CCTGCTATTT GCAGCCTCTC TATCAAGACT
  181 AATACACCTC TTCCCGCATC GGCTGCCTGT GAGAGGCTTT TCAGCACTGC AGGATTGCTT
  241 TTCAGCCCCA AAAGAGCTAG GCTTGACACT AACAATTTTG AGAATCAGCT TCTACTGAAG
  301 TTAAATCTGA GGTTTTACAA CTTTGAGTAG CGTGTACTGG CATTAGATTG TCTGTCTTAT
  361 AGTTTGATAA TTAAATACAA ACAGTTCTAA AGCAGGATAA AACCTTGTAT GCATTTCATT
  421 TAATGTTTTT TGAGATTAAA AGCTTAAACA AGAATCTCTA GTTTTCTTTC TTGCTTTTAC
  481 TTTTACTTCC TTAATACTCA AGTACAATTT TAATGGAGTA CTTTTTTACT TTTACTCAAG
  541 TAAGATTTCT AGCCCAGAAT AACTTTTTAC CTTTTTAAAA TTTGAGGGAT AAAAAATTTT
  601 TTTCCCTCTA AGAAAAACTC TGTGTTGAAC ATCTCTCTCA CCTCTGTGGA GAAAAAAAAA
  661 ATTTTTTGTG AGAGAAAACT CTTTTTTTTT ACCCCCCCCT GTCGGGAAAA AAAAAAATTC
  721 CTCGTGGGAG AAAAAAAACC CCCCCGAAAC CCGGGGGGGG GGGGCCCCCA AGGGGGGGAA
  781 AGGGGGGGGG GGGCGCCAAC AAAAAAATTT TACCCCCCCC CTTTTTTTTG GGAAAAAAAG
  841 GGAAGGGGGC CCCTCTCGGG GGGGGGGGGG TGAGAAAAAA AAAAAACGCG GGGGGGCCAC
  901 ACAAAGGGGG GGGGGGGGGG GACTCCCCTC TATCTTCAAA AAGATTTTTT TTTTTTTTTG
  961 TGACAGAACA GAGAGAGAAC TCTCTCTTCT CTGTCATAAA AAAAGAAGAG AGGGGGGGGC
 1021 GCCCCCCTCT GCTATATATA CCACCACTTT TTGGTGTGTC GCAGCACCAA TAGTGTGGGT
 1081 CGTGCTTAGT CACTAGCCGG GCGCGTAAGA ATAGGATACG TCGACGAAGC CACAGAAGAT
1141 AATTATGATC CTC
//
Primer: pAxEF_R2
Sequence file: RDB15275_A7H9d.seq check
>D004498I6_A7H9_1_pAxEF_R2_G01_07_ABI08.ab1
    1 CCCCGGTAAA AATGCTTTAT TTGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
   61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
  121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  181 ATGATCCTCT AGATCAGATC TGCGAAGATA CGGCCACGGG TGCTCTTGAT CCTGTGGCTG
  241 ATTTTGGACT GTGCTGCTCG CAGCTGCTGA TGAATCACAT ACTTCCTCCA TTTTCTTCCA
  301 CTGATTGACT GTTATAATTT CCCTAATTTC CAGGTCAAGG TGCTGTGCAT TGTGGTAATA
  361 GATGTGACAT GACGTCACTT CCAAAGGACC AATGAACATG TCTGACCAAT TTCATATAAT
  421 GTGAAAACGA TTTTCATAGG CAGAATAAAT AACATTTAAA TTAAACTGGG CATCAGCGCA
  481 ATTCAATTGG TTTGGTAATA GCAAGGGAAA ATAGAATGAA GTGATCTCCA AAAAATAAGT
  541 ACTTTTTGAC TGTAAATAAA ATTGTAAGGA GTAAAAAGTA CTTTTTTTTC TAAAAAAATG
  601 TAATTAAGTA AAAGTAAAAG TATTGATTTT TAATTGTACT CAAGTAAAGT AAAAATCCCC
  661 AAAAATAATA CTTAAGTACA GTAATCAAGT AAAATTACTC AAGTACTTTA CACCTCTGGT
  721 TCTTGACCCC CTACCTTCAG CAAGCCCAGC AGATCCACTA GTTCTAGAGC GGCCGCCACC
  781 GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC
  841 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG
  901 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT
  961 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG
 1021 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTTCTCGCT
 1081 CACTGAACTC GCTGCGCTCG GTCGTTTCGG GCTGGCCGCA GCGTATCAGC TCACCTCAAG
1141 GGCGGTAATA CCGGGTTATC TCCACTCACA GGATATCTAG GGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

2021.02.18

GNP_filter3_RDBDEP_html_201222.pl