Resource data sheet
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tFucci(SA)2.2/pCSII-EF

Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15445
Resource name tFucci(SA)2.2/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC192888 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15445_A7Jvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15445_A7Jva.seq
>D05018A5_A7Jv_1_EF1a-C_F_C02_08_ABI24.ab1
    1 ACCATGCATT GCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA
  121 GCGGCCGCGG ATCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG
  181 AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG
  241 AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA
  301 AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA
  361 AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG
  421 GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG
  481 ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC
  541 CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA
  601 TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG
  661 GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC
  721 CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA
  781 TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT
  841 ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGCCCAG CCCCGCCAGG CCCGCACTCC
  901 GCGCCCCGGC CTCCGCTACC AGTGGCAGCC GCAAGCGCGC CCGCCCGCCC GCCGCCCCCG
  961 GACGCGACCA GGCCAGGCCA CCGGGCCCGC AGGAGACTGC GGGCTGTCGG TGGACGAGGT
 1021 TTCCAGCCCC AGTACCCCCC GAGCCCCAGA CATCCCAGCC CTGGCCGTCT CGGCAGAAGA
1081 TAAGAAATTC ACCTCGGCAG CCAAGGTTCA GCCGGCCCCA CCTTGAAACA TTCCGTTGA
//
Primer: mCherry-C_F RDB15445_A7Jvb.seq
>D5018A5_1_A7Jv_mCherry-C_F_A01_01_ABI08.ab1
    1 AACCGGAGAG CCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA
   61 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
  121 CCACCGGCGG CATGGACGAG CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGC
  181 CCAGCCCCGC CAGGCCCGCA CTCCGCGCCC CGGCCTCCGC TACCAGTGGC AGCCGCAAGC
  241 GCGCCCGCCC GCCCGCCGCC CCCGGACGCG ACCAGGCCAG GCCACCGGCC CGCAGGAGAC
  301 TGCGGCTGTC GGTGGACGAG GTTTCCAGCC CCAGTACCCC CGAGGCCCCA GACATCCCAG
  361 CCTGCCCTTC TCCGGGCCAG AAGATAAAGA AATCCACCCC GGCAGCAGGT CAGCCGCCCC
  421 ACCTGACATC CGCGCAGGAC CAGGACACCA TCACCGGTCG CGCCAAGCGC GGCAGCGGCG
  481 CCACCAACTT CAGCCTGCTG AAGCAGGCCG GCGACGTGGA GGAGAACCCC GGCCCCCGCG
  541 AATTCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
  601 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA
  661 CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC
  721 CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA
  781 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGTACCA
  841 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA
  901 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG
  961 GGCACAAGCT GGGAGTACAA CTACATCAGC GACAACGTCT ATATCACCGC CCGACAAGCA
 1021 GAAGAACGGC ATCAAGGCCA CTTTCAGATC CCGCACAACA TCGAGGACGC CGCGGTGCAG
 1081 CTCGTCGAAC ACTAACAAGC AGAACACCCC CCCATTCGGG CGACGGACCC CCGAGTGCCA
1141 T
//
Primer: PRE_rev RDB15445_A7Jvc.seq
>D05018A5_A7Jv_1_PRE_rev_D02_11_ABI24.ab1
    1 ACGCTTTCAC ATAGCGTAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC
  121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA
  181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT
  241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT
  301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC
  361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT
  421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCC
  481 TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC
  541 ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT CAGGTAGTGG
  601 TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG
  661 AGCTGCACGC CGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC
  721 TTCTGCTTGT CGGCGGTGAT ATAGACGTTG TCGCTGATGT AGTTGTACTC CAGCTTGTGC
  781 CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG
  841 GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG
  901 ATGGTACGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC
  961 ATGTGGTCGG GGTAGCGGGC GAAGCACTGC ACGCCCCAGC TCAGGGTGGT CACGAGGGTG
 1021 GGCCCAGGGC ACGGGCAGCT TGCCGTGGTG CAGATGAACT TTCAAGGTTC AGCTGCCGTT
 1081 AGTGGCATCG CCGTAGGCCT TCGCCGGACA CGCCTGGAAC CTTGTGGATG ATTTTACGTT
1141 CG
//
Primer: CMV-Forward RDB15445_A7Jvd.seq
>D05018A5_A7Jv_1_CMV-Forward_E02_14_ABI24.ab1
    1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT
  781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
  841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA
  901 GAGAGAAAAA AAGAGCAGTG GGAAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
  961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGGTA CAGGCCAGAC AATTATTGTC
 1021 TGGGTATAGT GCCAGCAGCA GAACAATTTG CTGAGGCTAT TGAGGCGCAC CAGCCATCTG
1081 ATGCACTCAC AGGTCCTGGG GCATTCAAGC AAGCTTCCAG GGCCAG
//
Primer: BGH_rev2 RDB15445_A7Jve.seq
>D05018A5_A7Jv_1_BGH_rev2_F02_17_ABI24.ab1
    1 CTAAGTCAGC TTCAGAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
  121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
  181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
  241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
  301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
  361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
  421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
  481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
  541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
  601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
  661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
  721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
  781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
  841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
  901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
  961 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT TCCCCGACAC ACCACGGGAA TTGTCAGTGC
 1021 CCAACAGCCG AGCCTCCTGT CCAGCAGCGG GCAGCAGCGG CGATGAGTTC CGCCGTTGTA
 1081 TTAGGAGGGG AAAGCGAAAA GTCCTGCATG AAGCCTGGAC AGTGGATGGC ATTGACCCCT
1141 AATCCATTGG GGGGT
//
Primer: SV40pro_F RDB15445_A7Jvf.seq
>D05018A5_A7Jv_1_SV40pro_F_G02_20_ABI24.ab1
    1 ACTGTTGCTT GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
  121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
  181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
  241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
  301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
  361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
  421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
  481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
  541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
  661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
  841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
  901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
  961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
 1021 AATGAATCGG CCAACGCGCG GGGAGAGGCG GGTTGCGTAT TGGGGCGCTC TTCGCTTCTC
 1081 GCTCACTGAC TCGCTGCGCT CGATCGGATC GCTGCCGGCC GAGCCGGTAT TCCAGCCCTC
1141 CACCTCTCA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2017.12.11

GNP_filter3_RDBDEP_html_171106.pl