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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(SA)2.2/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15445
Resource name tFucci(SA)2.2/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC192888 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
         
        Sequence (full) RDB15445zzt01.seq compiled by us
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15445 tFucci(SA)2.2/pCSII-EF DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15445_A7Jvp1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EF1a-C_F
        Region: EF-1alpha pro, insert 5'
        Sequence file: RDB15445_A7Jvh.seq check
        >D05018A5_A7Jv_1_EF1a-C_F_C02_08_ABI24.ab1
            1 ACCATGCATT GCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
           61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA
          121 GCGGCCGCGG ATCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG
          181 AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG
          241 AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA
          301 AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA
          361 AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG
          421 GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG
          481 ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC
          541 CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA
          601 TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG
          661 GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC
          721 CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA
          781 TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT
          841 ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGCCCAG CCCCGCCAGG CCCGCACTCC
          901 GCGCCCCGGC CTCCGCTACC AGTGGCAGCC GCAAGCGCGC CCGCCCGCCC GCCGCCCCCG
          961 GACGCGACCA GGCCAGGCCA CCGGGCCCGC AGGAGACTGC GGGCTGTCGG TGGACGAGGT
         1021 TTCCAGCCCC AGTACCCCCC GAGCCCCAGA CATCCCAGCC CTGGCCGTCT CGGCAGAAGA
        1081 TAAGAAATTC ACCTCGGCAG CCAAGGTTCA GCCGGCCCCA CCTTGAAACA TTCCGTTGA
        //
        Primer: mCherry-C_F
        Region: insert mid
        Sequence file: RDB15445_A7Jvi.seq check
        >D5018A5_1_A7Jv_mCherry-C_F_A01_01_ABI08.ab1
            1 AACCGGAGAG CCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA
           61 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
          121 CCACCGGCGG CATGGACGAG CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGC
          181 CCAGCCCCGC CAGGCCCGCA CTCCGCGCCC CGGCCTCCGC TACCAGTGGC AGCCGCAAGC
          241 GCGCCCGCCC GCCCGCCGCC CCCGGACGCG ACCAGGCCAG GCCACCGGCC CGCAGGAGAC
          301 TGCGGCTGTC GGTGGACGAG GTTTCCAGCC CCAGTACCCC CGAGGCCCCA GACATCCCAG
          361 CCTGCCCTTC TCCGGGCCAG AAGATAAAGA AATCCACCCC GGCAGCAGGT CAGCCGCCCC
          421 ACCTGACATC CGCGCAGGAC CAGGACACCA TCACCGGTCG CGCCAAGCGC GGCAGCGGCG
          481 CCACCAACTT CAGCCTGCTG AAGCAGGCCG GCGACGTGGA GGAGAACCCC GGCCCCCGCG
          541 AATTCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
          601 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA
          661 CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC
          721 CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA
          781 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGTACCA
          841 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA
          901 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG
          961 GGCACAAGCT GGGAGTACAA CTACATCAGC GACAACGTCT ATATCACCGC CCGACAAGCA
         1021 GAAGAACGGC ATCAAGGCCA CTTTCAGATC CCGCACAACA TCGAGGACGC CGCGGTGCAG
         1081 CTCGTCGAAC ACTAACAAGC AGAACACCCC CCCATTCGGG CGACGGACCC CCGAGTGCCA
        1141 T
        //
        Primer: PRE_rev
        Region: PRE, insert 3'
        Sequence file: RDB15445_A7Jvj.seq check
        >D05018A5_A7Jv_1_PRE_rev_D02_11_ABI24.ab1
            1 ACGCTTTCAC ATAGCGTAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
           61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC
          121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA
          181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT
          241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT
          301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC
          361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT
          421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCC
          481 TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC
          541 ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT CAGGTAGTGG
          601 TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG
          661 AGCTGCACGC CGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC
          721 TTCTGCTTGT CGGCGGTGAT ATAGACGTTG TCGCTGATGT AGTTGTACTC CAGCTTGTGC
          781 CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG
          841 GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG
          901 ATGGTACGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC
          961 ATGTGGTCGG GGTAGCGGGC GAAGCACTGC ACGCCCCAGC TCAGGGTGGT CACGAGGGTG
         1021 GGCCCAGGGC ACGGGCAGCT TGCCGTGGTG CAGATGAACT TTCAAGGTTC AGCTGCCGTT
         1081 AGTGGCATCG CCGTAGGCCT TCGCCGGACA CGCCTGGAAC CTTGTGGATG ATTTTACGTT
        1141 CG
        //
        Primer: CMV-Forward
        Region: CMV pro, 5'LTR, HIV-1 psi
        Sequence file: RDB15445_A7Jvk.seq check
        >D05018A5_A7Jv_1_CMV-Forward_E02_14_ABI24.ab1
            1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
           61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
          121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
          181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
          241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
          301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
          361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
          421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
          481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
          541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
          601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
          661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
          721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT
          781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
          841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA
          901 GAGAGAAAAA AAGAGCAGTG GGAAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
          961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGGTA CAGGCCAGAC AATTATTGTC
         1021 TGGGTATAGT GCCAGCAGCA GAACAATTTG CTGAGGCTAT TGAGGCGCAC CAGCCATCTG
        1081 ATGCACTCAC AGGTCCTGGG GCATTCAAGC AAGCTTCCAG GGCCAG
        //
        Primer: EF1a-N_R
        Region: cPPT+CTS
        Sequence file: RDB15445_A7Jvl.seq check
        >15445_15445_A9IqS_EF1a-N_R_A02_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NGGCGNNGTG CGCTCTGCCC NCTGNCGGGC ACCGGANCCT
           61 CACGAAGCTT GAATTAATTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA CCCGAAAATT
          121 TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGTTG CTATTATGTC
          181 TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA AATTGTGGAT
          241 GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG TCTCTCTCTC
          301 CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG AGGTGGGTCT
          361 GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT ACAGCAAAAA
          421 CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TAATTTTATA TACCACAGCC
          481 AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTCC AATAATTCTT
          541 GTTCATTCTT TTCTTGCTGG TTTTGCGATT CTTCAATTAA GGAGTGTATT AAGCTTGTGT
          601 AATTGTTAAT TTCTCTGTCC CACTCNATCC AGGTCGTGTG ATTCCAAATC TGTTCCAGAG
          661 ATTTATTACT CCAACTAGCA TTCCAAGGCA CAGCAGTGGT GCAAATGAGT TTTCCAGAGC
          721 AACCCCNAAT CCCCAGGAGC TGTTGATCCT TTANGTATCT TTCCACAGCC AGGANTCTTG
          781 CCTGGAGCTG CTTGATGCCC CAGACTGTGA GTTGCAACAG ANGCTGTTGC GCCTCAATAG
          841 CCCTCANCAA ATTGTTCTGC TGCTGCACTA TACCAGANNA TAATTGTCTG GNCNGTANCG
          901 TCAGCGNCAT TGANGCTGCG CCCATAGNGC TNNCTGCTGC TNCCAAGANN CNNANGGAAC
          961 AAAGCTCCTA NTCCCNCNGC NCTTTTTTTC NCNNNGCACC ACNNNTCTNN TNNNNNNNGN
         1021 NGGNNNNNCT CCNNNNNGNN NNNNNTTTNN NNNNNTNNNN NNTNNNNNNN NNTNNNNNTN
        1081 NNNNNNNNN
        //
        Primer: BGH_rev2
        Region: BGH p(A), 3'LTR
        Sequence file: RDB15445_A7Jvm.seq check
        >D05018A5_A7Jv_1_BGH_rev2_F02_17_ABI24.ab1
            1 CTAAGTCAGC TTCAGAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
           61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
          121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
          181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
          241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
          301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
          361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
          421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
          481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
          541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
          601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
          661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
          721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
          781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
          841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
          901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
          961 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT TCCCCGACAC ACCACGGGAA TTGTCAGTGC
         1021 CCAACAGCCG AGCCTCCTGT CCAGCAGCGG GCAGCAGCGG CGATGAGTTC CGCCGTTGTA
         1081 TTAGGAGGGG AAAGCGAAAA GTCCTGCATG AAGCCTGGAC AGTGGATGGC ATTGACCCCT
        1141 AATCCATTGG GGGGT
        //
        Primer: SV40pro_F
        Region: SV40 pro, BleoR, SV40 p(A)
        Sequence file: RDB15445_A7Jvn.seq check
        >D05018A5_A7Jv_1_SV40pro_F_G02_20_ABI24.ab1
            1 ACTGTTGCTT GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
           61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
          121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
          181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
          241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
          301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
          361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
          421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
          481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
          541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
          601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
          661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
          721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
          781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
          841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
          901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
          961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
         1021 AATGAATCGG CCAACGCGCG GGGAGAGGCG GGTTGCGTAT TGGGGCGCTC TTCGCTTCTC
         1081 GCTCACTGAC TCGCTGCGCT CGATCGGATC GCTGCCGGCC GAGCCGGTAT TCCAGCCCTC
        1141 CACCTCTCA
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl