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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS coupler 3 (#RDB15128)

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15128 pBS coupler 3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS coupler 3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15128).

Reference section:

Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15128_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13R (Pr0160)
Region: insert, f1 ori
Sequence file: RDB15128_A7H9a.seq check
>D04498B4_A7H9_1_M13R_G10_19_ABI24.ab1
    1 ATGGGGCCAT TAACCTCACT AAGGGACAAA AGCTGGGTAC CGGGCCCCCC CTCGAGGTCG
   61 ACGGTATCGA TAAGCTTGAT GGTGGAGGCG GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG
  121 GATCGCCATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT TCTAGAGCGG CCGCCACCGC
  181 GGTGGAGCTC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT
  241 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC
  301 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT
  361 TGCGCAGCCT GAATGGCGAA TGGAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT
  421 AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA
  481 TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
  541 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG
  601 CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT
  661 AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT
  721 GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC
  781 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC
  841 AGGTGGCACT TTTTCGGGGG AAATGTGCGC GGAACCCCCT ATTTGTTTAT TTTTTCTAAA
  901 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCCT GATAAATGCT TCAATAATAT
  961 TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTTATTC CCTTTTTTTT
 1021 TGCGGCATTT TTGCCCTTCC CTGTTTTTTG CTCCACCCAG AAACGCTTGG GTGAAAGGTT
1081 AAAGGATGGC TTGAAAGAAT CAGGTTGGGG GTGCCAACCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]