Sequencing and PCR primers for primer information.
Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
| Catalog number | RDB15128 |
|---|---|
| Resource name | pBS coupler 3 |
| Clone info. | Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. |
| Vector backbone | Plasmid |
| Depositor | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word] |
| Remarks | Please obtain written approval of the depositor. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15128 | pBS coupler 3 | DNA solution |
Electronic file
Original reference
| original | Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. |
|---|
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dr. Miyawaki's resources (English text) |
|---|---|
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15128_A7H9p1-1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Region: insert, f1 ori | Primer: M13R | Sequence: RDB15128_A7H9a.seq |
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|
>D04498B4_A7H9_1_M13R_G10_19_ABI24.ab1 1 ATGGGGCCAT TAACCTCACT AAGGGACAAA AGCTGGGTAC CGGGCCCCCC CTCGAGGTCG 61 ACGGTATCGA TAAGCTTGAT GGTGGAGGCG GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG 121 GATCGCCATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT TCTAGAGCGG CCGCCACCGC 181 GGTGGAGCTC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT 241 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC 301 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 361 TGCGCAGCCT GAATGGCGAA TGGAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT 421 AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA 481 TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC 541 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG 601 CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT 661 AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT 721 GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC 781 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC 841 AGGTGGCACT TTTTCGGGGG AAATGTGCGC GGAACCCCCT ATTTGTTTAT TTTTTCTAAA 901 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCCT GATAAATGCT TCAATAATAT 961 TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTTATTC CCTTTTTTTT 1021 TGCGGCATTT TTGCCCTTCC CTGTTTTTTG CTCCACCCAG AAACGCTTGG GTGAAAGGTT 1081 AAAGGATGGC TTGAAAGAAT CAGGTTGGGG GTGCCAACCG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. |
|---|
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl