Please review the QC test results indicated by icon below as well as clone information before placing your order.

Flamindo/pcDNA3

Expression vector of yellow fluorescent biosensor for cAMP, Flamindo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A. (2013) Extracellular Calcium influx activates Adenylate Cyclase 1 and potentiates Insulin secretion in MIN6 cells. Biochem. J., 450 (2): 365-373. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15247_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert5' Sequence file: RDB15247_A7H9a.seq
>D04498F5-2_A7H9_CMV-Forward_G08_20_ABI24.ab1     1 TGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC   121 TCACTATAGG GAGACCCAAG CTTATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG   181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG   241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA   301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCCTG ATGTGCTTCG   361 CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT   421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG   481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG   541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACCT CCGCGGGGAG GAGCTTGTTG   601 AGGCTATGGC CCTCCTGTCC CAGCGAGGGC CTGATGCCCT ACTCACCGTG GCGCTCCGGA   661 AGCCCCCAGG TCAGCGTACG GATGAAGAAC TGGACCTGAT CTTTGAAGAG CTGCTGCATA   721 TCAAGGCGGT GGCACACCTT TCTAACTCGG TGAAGCGGGA ACTAGCTGCT GTTCTGCTCT   781 TTGAACCACA CAGCAAGGCA GGAACTGTGT TGTTCAGCCA GGGGGACAAG GGTACCTCGT   841 GGTACATTAT CTGGAAGGGA TCTGTCAATG TGGTGACCCA TGGCAAGGGG GCTGGTGACC   901 ACGTTGCACG AGGGAGATGA CTTTGGACAG CTGGCTCTGG TGAACGACGC ACCTCGGGCA   961 GCCACCATCA TCCTTCGAGA AAAATAACTG TCACTTTCTG CGTGTGGACA AGCAGGACTT  1021 TCAACCGCAT CATCAAAGGA TGTGGAAAGC CAAAAACCCA TGAGACTGGT AAGCAGGATC 1081 ACCAGCCACA ACCGTCTATA TCAATTGACC GAACAGTCGA GGATCGGTCA TTCAAAATG //
Primer: BGH_rev2 (Pr0606) Region: bGHp(A),insert3' Sequence file: RDB15247_A7H9b.seq
>D04498F5-2_A7H9_BGH_rev2_H08_23_ABI24.ab1     1 CTCAAGTAGT ATGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG   121 CATGCTCGAG TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA   181 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGT   241 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT   301 GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA   361 CCTTGATGCC GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGAATTCTT   421 CTTCCAGTCT CATGGTTTTT GCTTCCACAT CCTTGATGAT GCGGTTGAAG TCCTGCTTGT   481 CCACACGCAG AAAGTGACAG TTATTTTCTC GAAGGATGAT GGTGGCTGCC CGAGGTGCGT   541 CGTTCACCAG AGCCAGCTGT CCAAAGTCAT CTCCCTCGTG CAACGTGGTC ACCAGCCCCT   601 TGCCATGGGT CACCACATTG ACAGATCCCT TCCAGATAAT GTACCACGAG GTACCCTTGT   661 CCCCCTGGCT GAACAACACA GTTCCTGCCT TGCTGTGTGG TTCAAAGAGC AGAACAGCAG   721 CTAGTTCCCG CTTCACCGAG TTAGAAAGGT GTGCCACCGC CTTGATATGC AGCAGCTCTT   781 CAAAGATCAG GTCCAGTTCT TCATCCGTAC GCTGACCTGG GGGCTTCCGG AGCGCCACGG   841 TGAGTAGGGC ATCAGGCCCT CGCTGGGACA GGAGGGCCAT AGCCTCAACA AGCTCCTCCC   901 CGCGGAGGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC   961 CCTTCAGCTC GATGCGGGTT CACCAGGGTG TCGCCCTCGA ACTTTCACCT CGGCGCGGGT  1021 CTTGTTAGAT GCGTCGTCCT TGAGAGATGG TTGCGCCTCC TTGACGTAGC CTTTCGGCAT 1081 GGCCGAACTG CAGAAGGTCG TGCCTGCCTT TCAATGCTGG ATTCCCG //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori,NeoR Sequence file: RDB15247_A7H9c.seq
>D04498F5-2_A7H9_SV40pro_F_A09_03_ABI24.ab1     1 CCTGGTCCTG GGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA    61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG   121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG   661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG   961 CCCAACCCTG CCCATCACGA GATTTCGATT TCCACCGCCG CCCTTCTATG AAAGGTTGGG  1021 CTTTCGGAAT TCGCTTTCCA GGACGCCGGC TGGATGAATC TTCAAGCGCG GGATCTCAAT 1081 GGCTGAGTAT CGCCGACCCG ACCTGTTTAT TGGCAGGCCT TTAGTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl