Expression vector of yellow fluorescent biosensor for cAMP, Flamindo.
Clone info. | Expression vector of yellow fluorescent cAMP indicator, Flamindo. A variant of YFP, Citrine was interrupted by cAMP-binding domain of mouse Epac1 (157-316aa). For the compositon, refer Fig.1 of Kitaguchi, T., Biochem. J. 450: 365-373, 2013. |
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Comment | Use Flamindo (R279E)/pcDNA3 (RDB15248) as a negative control. |
Vector backbone | pcDNA3 (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
Growth conditions | 37oC, LB + Amp |
Gene/insert name | mouse Epac1 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Miyawaki, Atsushi | |
Sequence (full) | RDB15247hts01.seq ![]() |
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Publication | Kitaguchi, T., Biochem. J. 450 (2): 365-373 (2013). [link to RRC of NBRP] |
Test sheet | RDB15247_B0Bip1-2.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Kitaguchi, T. et al., Biochem. J., 450 (2): 365-373 (2013). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Kitaguchi, T. et al., Biochem. J., 450 (2): 365-373 (2013). |
備考 | 提供条件は英文のまま記載してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15247 | Flamindo/pcDNA3 | DNA solution |
Materials & Methods section:
The Flamindo/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15247). |
Reference section:
Kitaguchi, T., Oya, M., Wada, Y., Tsuboi, T., Miyawaki, A., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. [link to RRC of NBRP] |
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reference | Odaka, H., Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One 9 (6): e100252 (2014). PMID 24959857. |
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