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Flamindo/pcDNA3 (#RDB15247)

Expression vector of yellow fluorescent biosensor for cAMP, Flamindo.

Clone info. Expression vector of yellow fluorescent cAMP indicator, Flamindo. A variant of YFP, Citrine was interrupted by cAMP-binding domain of mouse Epac1 (157-316aa). For the compositon, refer Fig.1 of Kitaguchi, T., Biochem. J. 450: 365-373, 2013.
Comment Use Flamindo (R279E)/pcDNA3 (RDB15248) as a negative control.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37oC, LB + Amp
Gene/insert name mouse Epac1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15247hts01.seq checkAssembled from experimentally sequenced data.
Publication Kitaguchi, T., Biochem. J. 450 (2): 365-373 (2013). [link to RRC of NBRP]
Test sheet RDB15247_B0Bip1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Kitaguchi, T. et al., Biochem. J., 450 (2): 365-373 (2013).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Kitaguchi, T. et al., Biochem. J., 450 (2): 365-373 (2013).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15247 Flamindo/pcDNA3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The Flamindo/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15247).

Reference section:

Kitaguchi, T., Oya, M., Wada, Y., Tsuboi, T., Miyawaki, A., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kitaguchi, T., Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells. Biochem. J. 450 (2): 365-373 (2013). PMID 23282092. [link to RRC of NBRP]
reference Odaka, H., Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One 9 (6): e100252 (2014). PMID 24959857.