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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Flash pericam/pcDNA3

Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM.

Catalog number RDB15139
Resource name Flash pericam/pcDNA3
Clone info. Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM. Better at 28-30oC than 37oC. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          
         
        Sequence (partial) RDB15139pka02.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15139 Flash pericam/pcDNA3 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15139_A7H9p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro, T7 pro, insert 5'
        Sequence file: RDB15139_A7H9a.seq check
        >D04498C6_A7H9_1_CMV-Forward_B02_05_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNTNG NNGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
           61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
          121 CGACTCACTA TAGGGAGACC CAAGCTTGCG GCCGCCACCA TGGTGAAGAG GCGCTGGAAG
          181 AAAAACTTCA TTGCCGTCAG CGCTGCCAAC CGGTTCAAGA AGATCTCCAG CTCCGGGGCA
          241 CTGGGGTCTG CAGGCTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
          301 GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC
          361 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
          421 TACCTGAGCC ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
          481 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT
          541 GGCAGCGGTG GCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG
          601 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC
          661 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG
          721 CCCTGNCCCA CCCTCGTGAN CACCTTCGGC TACGGCCTGA AGTGCTTCGC CCGCTACCCC
          781 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAN
          841 CGCACCATCT TCTTCNAGGA CGACGGCAAC TACAAGANCC GCGCCGANGT GAANTNCGAG
          901 GGCGACACCC NGGNGAACCG CATCGAGCTN AANGGCATCG NNTTCNAGGA NGNNNGNANC
        961 NTCCNNGGGN NNNNNCNNGN NNTANNNCNG NNACNNGNNN GNNNNNNCNN NNNNNNNN
        //
        Primer: BGH_rev2
        Region: insert 3'
        Sequence file: RDB15139_A7H9b.seq check
        >D04498C6_A7H9_1_BGH_rev2_D06_12_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNANNN NNNNNNGGCT GGCAANNNNN
           61 NNGNACAGTC GAGGNNNNNN NNNNNNNCTC TAGCATTTAG GTGACACTAT AGAATAGGGC
          121 CCNCTANATG CATGCTCGAG CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTACTTT
          181 GCTGTCATCA TTTGTACAAA CTCTTCATAG TTTACTTGGC CATCACCATC GATATCTGCT
          241 TCCCTTATCA TTTCATCAAC TTCTTCATCT GTTAACTTCT CCCCGAGGTT TGTCATGACG
          301 TGACGTAACT GAGCAGCGCT GATGTAGCCG TTCCCATCCT TGTCAAAAAC ACGGAATGCT
          361 TCTCGGATTT CCTCTTCGCT GTCTGTGTCC TTCATTTTTC TAGCCATCAT AGTAAGAAAT
          421 TCAGGAAAGT AAATCGTTCC ATTGCCATCA GCATCGACTT CATTGATCAT ATCCTGCAAT
          481 TCTGCTTCCG TTGGGTTTTG TCCAAGCGAC CTCATAACGG TGCCAAGTTC CTTTGTGGTG
          541 ATGGTGCCGT CCCCATCCTT GTCGAATAAT GAGAAGGCTT CTTTGAACTC TGCNNNNNNC
          601 TCTTCTGTCA NTTGGTCATG CATCCCNGNN NNGTTGTACT CCAGCTTGTG CCCCANNNTG
          661 TTGCCGTCCT CCTTGAANTC GATGCCCTTC AGCTCGATGC GGTTCNCCAG GGTGTCGCCC
          721 TCNAACNNNN NNTCNGCGCG GGTCTTGTAN TTGNNNNCGT CCTTGAAGAA GANGGGTGCG
          781 CTCCTGGACN NNNNNCTTCN GGNNNTGGCG GNACTTGAAG AANNTCGTGC TGCTTCATGT
          841 GGTCNGGGGT ANCGGGCGAA GCACTTCANG NCNGNANCCN NNNNGNGGTC ACNNANGGTG
          901 GGCNNNGGCA CGGGCAGCTT GCCGGTGGTG CANNNGAAAC TTCNNGGGTC NNCNNGNCNG
          961 NANGNNNCNN CNCCCTNNNC CTCNCNNNAN NNNCTGAACT TGNGGNCNNN TTTANNNNNN
        1021 NNNGNNNNNN NNNN
        //
        Primer: EGFP-N_F
        Region: insert mid
        Sequence file: RDB15139_A7H9c.seq check
        >D04498C6_A7H9_1_EGFP-N_F_G07_19_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN CNNNGGCGNN GCNNCCTACG GCAAGCTGNN CCTGAAGTTC
           61 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CTTCGGCTAC
          121 GGCCTGAAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
          181 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
          241 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
          301 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACGGTACC
          361 GGGATGCATG ACCAACTGAC AGAAGAGCAG ATTGCAGAGT TCAAAGAAGC CTTCTCATTA
          421 TTCGACAAGG ATGGGGACGG CACCATCACC ACAAAGGAAC TTGGCACCGT TATGAGGTCG
          481 CTTGGACAAA ACCCAACGGA AGCAGAATTG CAGGATATGA TCAATGAAGT CGATGCTGAT
          541 GGCAATGGAA CGATTTACTT TCCTGAATTT CTTACTATGA TGGCTAGAAA AATGAAGGAC
          601 ACAGACAGCG AAGAGGAAAT CCGAGAAGCA TTCCGTGTTT TTGACAAGGA TGGGAACGGC
          661 TACATCAGCG CTGCTCAGTT ACGTCACGTC ATGACAAACC TCGGGGAGAA GTTAACAGAT
          721 GAAGAAGTTG ANGAAATGAT AANGGAAGCA GATATCGATG GTGATGGCCA AGTAAACTAT
          781 GAAGAGTTTG TACNAATGNN GACAGCAAAG TAAGAATTCT GCAGATATCC ATCACACTGG
          841 CGGNNGCTCN NNCATGNATC TANAGGGNCC TATTCTATAN TGTCANNNAA ATGCTANNNC
          901 TCGNNNNNCA NCNNCNNNNG NNCNNTCTAN NNNNNANCNN TCTNNNNNTN GNCCNNNCCC
        961 NNNNNNNNNC NNTNNNNCNN NNNNNNNN
        //
        Primer: SV40pro_ori_F
        Region: NeoR
        Sequence file: RDB15139_A7H9d.seq check
        >D04498C6_A7H9_1_SV40pro_ori_F_H07_22_ABI24.ab1
            1 NNNNNNNNNN NNNGNCGCCT CNGCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
           61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
          121 TCAAGAGACA GGNTGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
          181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
          241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
          301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
          361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
          421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
          481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
          541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
          601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
          661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGNNCTC GTCGTGACCC ATGGCGATGC
          721 CTGCTTGCCG AATATCATGG TGGANNATGG CCGCTTTTCT GGANTCATCG ACTGTGGCCG
          781 GCTGNNTGTG GCGGANCGCT ATCANGNCAT ANCGTTGGCT ANCCGTGATA TTGCTGAAGA
          841 NCTTGGCGGC GAATGGGCTG ANCGCTTCCT CGNNCTTTAC NGNANCGCNN CNCCNNATTN
        901 NCANCNNATC NNCNTTCNNN CGNCNTTNNN GNCNANNTNN NNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        2022.07.27

        GNP_filter3_RDBDEP_html_220727.pl