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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Flash pericam/pcDNA3

Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM.

Catalog number RDB15139
Resource name Flash pericam/pcDNA3
Clone info. Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM. Better at 28-30oC than 37oC. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15139 Flash pericam/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB15139pka02.seq

Original reference

original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15139_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, T7 pro, insert 5'
Sequence file: RDB15139_A7H9a.seq check
>D04498C6_A7H9_1_CMV-Forward_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNG NNGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGCG GCCGCCACCA TGGTGAAGAG GCGCTGGAAG
  181 AAAAACTTCA TTGCCGTCAG CGCTGCCAAC CGGTTCAAGA AGATCTCCAG CTCCGGGGCA
  241 CTGGGGTCTG CAGGCTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
  301 GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC
  361 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
  421 TACCTGAGCC ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
  481 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT
  541 GGCAGCGGTG GCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG
  601 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC
  661 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG
  721 CCCTGNCCCA CCCTCGTGAN CACCTTCGGC TACGGCCTGA AGTGCTTCGC CCGCTACCCC
  781 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAN
  841 CGCACCATCT TCTTCNAGGA CGACGGCAAC TACAAGANCC GCGCCGANGT GAANTNCGAG
  901 GGCGACACCC NGGNGAACCG CATCGAGCTN AANGGCATCG NNTTCNAGGA NGNNNGNANC
961 NTCCNNGGGN NNNNNCNNGN NNTANNNCNG NNACNNGNNN GNNNNNNCNN NNNNNNNN
//
Primer: BGH_rev2
Region: insert 3'
Sequence file: RDB15139_A7H9b.seq check
>D04498C6_A7H9_1_BGH_rev2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNANNN NNNNNNGGCT GGCAANNNNN
   61 NNGNACAGTC GAGGNNNNNN NNNNNNNCTC TAGCATTTAG GTGACACTAT AGAATAGGGC
  121 CCNCTANATG CATGCTCGAG CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTACTTT
  181 GCTGTCATCA TTTGTACAAA CTCTTCATAG TTTACTTGGC CATCACCATC GATATCTGCT
  241 TCCCTTATCA TTTCATCAAC TTCTTCATCT GTTAACTTCT CCCCGAGGTT TGTCATGACG
  301 TGACGTAACT GAGCAGCGCT GATGTAGCCG TTCCCATCCT TGTCAAAAAC ACGGAATGCT
  361 TCTCGGATTT CCTCTTCGCT GTCTGTGTCC TTCATTTTTC TAGCCATCAT AGTAAGAAAT
  421 TCAGGAAAGT AAATCGTTCC ATTGCCATCA GCATCGACTT CATTGATCAT ATCCTGCAAT
  481 TCTGCTTCCG TTGGGTTTTG TCCAAGCGAC CTCATAACGG TGCCAAGTTC CTTTGTGGTG
  541 ATGGTGCCGT CCCCATCCTT GTCGAATAAT GAGAAGGCTT CTTTGAACTC TGCNNNNNNC
  601 TCTTCTGTCA NTTGGTCATG CATCCCNGNN NNGTTGTACT CCAGCTTGTG CCCCANNNTG
  661 TTGCCGTCCT CCTTGAANTC GATGCCCTTC AGCTCGATGC GGTTCNCCAG GGTGTCGCCC
  721 TCNAACNNNN NNTCNGCGCG GGTCTTGTAN TTGNNNNCGT CCTTGAAGAA GANGGGTGCG
  781 CTCCTGGACN NNNNNCTTCN GGNNNTGGCG GNACTTGAAG AANNTCGTGC TGCTTCATGT
  841 GGTCNGGGGT ANCGGGCGAA GCACTTCANG NCNGNANCCN NNNNGNGGTC ACNNANGGTG
  901 GGCNNNGGCA CGGGCAGCTT GCCGGTGGTG CANNNGAAAC TTCNNGGGTC NNCNNGNCNG
  961 NANGNNNCNN CNCCCTNNNC CTCNCNNNAN NNNCTGAACT TGNGGNCNNN TTTANNNNNN
1021 NNNGNNNNNN NNNN
//
Primer: EGFP-N_F
Region: insert mid
Sequence file: RDB15139_A7H9c.seq check
>D04498C6_A7H9_1_EGFP-N_F_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNNGGCGNN GCNNCCTACG GCAAGCTGNN CCTGAAGTTC
   61 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CTTCGGCTAC
  121 GGCCTGAAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
  181 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
  241 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
  301 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACGGTACC
  361 GGGATGCATG ACCAACTGAC AGAAGAGCAG ATTGCAGAGT TCAAAGAAGC CTTCTCATTA
  421 TTCGACAAGG ATGGGGACGG CACCATCACC ACAAAGGAAC TTGGCACCGT TATGAGGTCG
  481 CTTGGACAAA ACCCAACGGA AGCAGAATTG CAGGATATGA TCAATGAAGT CGATGCTGAT
  541 GGCAATGGAA CGATTTACTT TCCTGAATTT CTTACTATGA TGGCTAGAAA AATGAAGGAC
  601 ACAGACAGCG AAGAGGAAAT CCGAGAAGCA TTCCGTGTTT TTGACAAGGA TGGGAACGGC
  661 TACATCAGCG CTGCTCAGTT ACGTCACGTC ATGACAAACC TCGGGGAGAA GTTAACAGAT
  721 GAAGAAGTTG ANGAAATGAT AANGGAAGCA GATATCGATG GTGATGGCCA AGTAAACTAT
  781 GAAGAGTTTG TACNAATGNN GACAGCAAAG TAAGAATTCT GCAGATATCC ATCACACTGG
  841 CGGNNGCTCN NNCATGNATC TANAGGGNCC TATTCTATAN TGTCANNNAA ATGCTANNNC
  901 TCGNNNNNCA NCNNCNNNNG NNCNNTCTAN NNNNNANCNN TCTNNNNNTN GNCCNNNCCC
961 NNNNNNNNNC NNTNNNNCNN NNNNNNNN
//
Primer: SV40pro_ori_F
Region: NeoR
Sequence file: RDB15139_A7H9d.seq check
>D04498C6_A7H9_1_SV40pro_ori_F_H07_22_ABI24.ab1
    1 NNNNNNNNNN NNNGNCGCCT CNGCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAAGAGACA GGNTGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
  541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
  601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
  661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGNNCTC GTCGTGACCC ATGGCGATGC
  721 CTGCTTGCCG AATATCATGG TGGANNATGG CCGCTTTTCT GGANTCATCG ACTGTGGCCG
  781 GCTGNNTGTG GCGGANCGCT ATCANGNCAT ANCGTTGGCT ANCCGTGATA TTGCTGAAGA
  841 NCTTGGCGGC GAATGGGCTG ANCGCTTCCT CGNNCTTTAC NGNANCGCNN CNCCNNATTN
901 NCANCNNATC NNCNTTCNNN CGNCNTTNNN GNCNANNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

2021.03.23

GNP_filter3_RDBDEP_html_210308.pl