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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Flash pericam/pcDNA3 (#RDB15139)

Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM.

Clone info. Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM. Better at 28-30oC than 37oC. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (partial) RDB15139pka02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15139 Flash pericam/pcDNA3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Flash pericam/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15139).

Reference section:

Nagai, T., Sawano, A., Park, E.S., Miyawaki, A., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15139_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, T7 pro, insert 5'
Sequence file: RDB15139_A7H9a.seq check
>D04498C6_A7H9_1_CMV-Forward_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNG NNGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGCG GCCGCCACCA TGGTGAAGAG GCGCTGGAAG
  181 AAAAACTTCA TTGCCGTCAG CGCTGCCAAC CGGTTCAAGA AGATCTCCAG CTCCGGGGCA
  241 CTGGGGTCTG CAGGCTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
  301 GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC
  361 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
  421 TACCTGAGCC ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
  481 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT
  541 GGCAGCGGTG GCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG
  601 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC
  661 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG
  721 CCCTGNCCCA CCCTCGTGAN CACCTTCGGC TACGGCCTGA AGTGCTTCGC CCGCTACCCC
  781 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAN
  841 CGCACCATCT TCTTCNAGGA CGACGGCAAC TACAAGANCC GCGCCGANGT GAANTNCGAG
  901 GGCGACACCC NGGNGAACCG CATCGAGCTN AANGGCATCG NNTTCNAGGA NGNNNGNANC
961 NTCCNNGGGN NNNNNCNNGN NNTANNNCNG NNACNNGNNN GNNNNNNCNN NNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: insert 3'
Sequence file: RDB15139_A7H9b.seq check
>D04498C6_A7H9_1_BGH_rev2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNANNN NNNNNNGGCT GGCAANNNNN
   61 NNGNACAGTC GAGGNNNNNN NNNNNNNCTC TAGCATTTAG GTGACACTAT AGAATAGGGC
  121 CCNCTANATG CATGCTCGAG CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTACTTT
  181 GCTGTCATCA TTTGTACAAA CTCTTCATAG TTTACTTGGC CATCACCATC GATATCTGCT
  241 TCCCTTATCA TTTCATCAAC TTCTTCATCT GTTAACTTCT CCCCGAGGTT TGTCATGACG
  301 TGACGTAACT GAGCAGCGCT GATGTAGCCG TTCCCATCCT TGTCAAAAAC ACGGAATGCT
  361 TCTCGGATTT CCTCTTCGCT GTCTGTGTCC TTCATTTTTC TAGCCATCAT AGTAAGAAAT
  421 TCAGGAAAGT AAATCGTTCC ATTGCCATCA GCATCGACTT CATTGATCAT ATCCTGCAAT
  481 TCTGCTTCCG TTGGGTTTTG TCCAAGCGAC CTCATAACGG TGCCAAGTTC CTTTGTGGTG
  541 ATGGTGCCGT CCCCATCCTT GTCGAATAAT GAGAAGGCTT CTTTGAACTC TGCNNNNNNC
  601 TCTTCTGTCA NTTGGTCATG CATCCCNGNN NNGTTGTACT CCAGCTTGTG CCCCANNNTG
  661 TTGCCGTCCT CCTTGAANTC GATGCCCTTC AGCTCGATGC GGTTCNCCAG GGTGTCGCCC
  721 TCNAACNNNN NNTCNGCGCG GGTCTTGTAN TTGNNNNCGT CCTTGAAGAA GANGGGTGCG
  781 CTCCTGGACN NNNNNCTTCN GGNNNTGGCG GNACTTGAAG AANNTCGTGC TGCTTCATGT
  841 GGTCNGGGGT ANCGGGCGAA GCACTTCANG NCNGNANCCN NNNNGNGGTC ACNNANGGTG
  901 GGCNNNGGCA CGGGCAGCTT GCCGGTGGTG CANNNGAAAC TTCNNGGGTC NNCNNGNCNG
  961 NANGNNNCNN CNCCCTNNNC CTCNCNNNAN NNNCTGAACT TGNGGNCNNN TTTANNNNNN
1021 NNNGNNNNNN NNNN
//
Primer: EGFP-N_F (Pr0649)
Region: insert mid
Sequence file: RDB15139_A7H9c.seq check
>D04498C6_A7H9_1_EGFP-N_F_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNNGGCGNN GCNNCCTACG GCAAGCTGNN CCTGAAGTTC
   61 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CTTCGGCTAC
  121 GGCCTGAAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
  181 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
  241 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
  301 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACGGTACC
  361 GGGATGCATG ACCAACTGAC AGAAGAGCAG ATTGCAGAGT TCAAAGAAGC CTTCTCATTA
  421 TTCGACAAGG ATGGGGACGG CACCATCACC ACAAAGGAAC TTGGCACCGT TATGAGGTCG
  481 CTTGGACAAA ACCCAACGGA AGCAGAATTG CAGGATATGA TCAATGAAGT CGATGCTGAT
  541 GGCAATGGAA CGATTTACTT TCCTGAATTT CTTACTATGA TGGCTAGAAA AATGAAGGAC
  601 ACAGACAGCG AAGAGGAAAT CCGAGAAGCA TTCCGTGTTT TTGACAAGGA TGGGAACGGC
  661 TACATCAGCG CTGCTCAGTT ACGTCACGTC ATGACAAACC TCGGGGAGAA GTTAACAGAT
  721 GAAGAAGTTG ANGAAATGAT AANGGAAGCA GATATCGATG GTGATGGCCA AGTAAACTAT
  781 GAAGAGTTTG TACNAATGNN GACAGCAAAG TAAGAATTCT GCAGATATCC ATCACACTGG
  841 CGGNNGCTCN NNCATGNATC TANAGGGNCC TATTCTATAN TGTCANNNAA ATGCTANNNC
  901 TCGNNNNNCA NCNNCNNNNG NNCNNTCTAN NNNNNANCNN TCTNNNNNTN GNCCNNNCCC
961 NNNNNNNNNC NNTNNNNCNN NNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR
Sequence file: RDB15139_A7H9d.seq check
>D04498C6_A7H9_1_SV40pro_ori_F_H07_22_ABI24.ab1
    1 NNNNNNNNNN NNNGNCGCCT CNGCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAAGAGACA GGNTGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
  541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
  601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
  661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGNNCTC GTCGTGACCC ATGGCGATGC
  721 CTGCTTGCCG AATATCATGG TGGANNATGG CCGCTTTTCT GGANTCATCG ACTGTGGCCG
  781 GCTGNNTGTG GCGGANCGCT ATCANGNCAT ANCGTTGGCT ANCCGTGATA TTGCTGAAGA
  841 NCTTGGCGGC GAATGGGCTG ANCGCTTCCT CGNNCTTTAC NGNANCGCNN CNCCNNATTN
901 NCANCNNATC NNCNTTCNNN CGNCNTTNNN GNCNANNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. [link to RRC of NBRP]