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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)2.1/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). CMV promoter.

Catalog number RDB15453
Resource name tFucci(CA)2.1/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of insert sequence LC212777 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Anemonia majano CFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
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        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15453 tFucci(CA)2.1/pCSII-CMV DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15453_A7Jvp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: mCherry-C_R
        Region: insert 5' (mCherry), CMV pro_2
        Sequence file: RDB15453_A7Jvg.seq check
        >D05018B4_2_A7Jv_mCherry-C_R_D01_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN GTTGNNGGCG CCGGGCANCT GCACGGGCTT
           61 CTTGGCCTTG TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
          121 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC
          181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG
          241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC
          301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG
          361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA
          421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT
          481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG
          541 GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT
          601 GTTATCCTCC TCGCCCTTGC TCACCATGGT GGATCCTATG CTAGCGGATC TGACGGTTCA
          661 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA
          721 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CNAAACAAAC
          781 TCCCATTGAC GTCNATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC
          841 GCNCATTGAT GTACTGCCNA AACCGCATCA CCATGGNAAT AGCGATGANT AANACGTANA
          901 TGTACTGCNN AGNNNNAAAG TNCNNTAANG NCATGTNCNG GNNNTNANGN CNNGGNGGNN
        961 NNTTTANNNN CATTGNNNNN NNNNN
        //
        Primer: PRE_rev
        Region: PRE, insert 3' (hGem(1_110), AmCyan1)
        Sequence file: RDB15453_A7Jvh.seq check
        >D05018B4_2_A7Jv_PRE_rev_E02_14_ABI24.ab1
            1 NNNNNNNNNN NGCGNNNNGG AGCANCATAG TTAAGANTAC CAGTCAATCT TTCNCAAATT
           61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA
          121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA
          181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG
          241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT
          301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT
          361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT
          421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCGA
          481 AGGGCACCAC GGAGGTGATG TGGGCCACGG CGTGCTCGGT CAGCTGCACG CTGTTGCCGC
          541 CCTTGTCCAG GTCGGTTCTG GCGATGCGGT GCTCCACCAC GTGGTTGGGG GGCATGGTCA
          601 CGGGCTTCTT GGTCTTGTAG GAGGTGTGGA ACTGGCATCT GTAGTTGCCG CCGCCCTGCA
          661 GCATCAGGAA GGCGGTCACG TCGCCCTTCA AGATGCCGTC GCACACGGTC ATCTTCTCGA
          721 ANGANGGGTC CCAGCCGGTG GTCTTCTTGG NCATCACGGG GCCGTCGGCG GGGAANTTCA
          781 CGCCGNGGAA NGTGGANTTG TGCTCNAAGC AGTTGCCCTT CNNNTGATCT CCCNGCTGGC
          841 GGTGGNCNCG CCGCCNNCCN CNNNNNGAAN GNTNNCTCGN NNNNNTGNCN NCGGGGANNN
          901 NCNGCTTNAA NNANNNNGNN NTGCTNNNGG GGNNNNNNNN NAANNANNNG NTNNNNNNAC
        961 NTGNNNNNNN NNN
        //
        Primer: mRFP1-inner1
        Region: insert mid (mCherry, hCdt1(1_100)Cy(-))
        Sequence file: RDB15453_A7Jvi.seq check
        >15453_15453_B1K5F_2_mRFP1-inner1_F02_17_ABI24.ab1
            1 NNNNNNNNNN NNNNNNCNNN GNNTCCTCCC TGCAGGANGG CGAGTTCATC TACAAGGTGA
           61 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT
          121 GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC
          181 AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG
          241 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
          301 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
          361 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGATGG
          421 AGCAGCGCCG CGTCACCGAC TTCTTCGCGC GCCGCCGCCC CGGGCCCCCC CGCATCGCGC
          481 CGCCCAAGCT GGCCTGCCGC ACCCCCAGCC CCGCCAGGCC CGCACTCCGC GCCCCGGCCT
          541 CCGCTACCAG TGGCAGCCGC AAGCGCGCCC GCCCGCCCGC CGCCCCCGGA NGCGNNCAGG
          601 CCAGGNCACC GGCCCGCGCG GCCGCGCGGC TGTCGGNGGA NNANGNTTCC AGCCCCAGTA
        661 CCCCCNANGC CCNNNANNTC CNNNNNNGNC CNNCTNNNGN NNNNNNNNN
        //
        Primer: mCherry-C_F
        Region: insert mid (mCherry, hCdt1(1_100)Cy(-), P2A, AmCyan1)
        Sequence file: RDB15453_A7Jvj.seq check
        >D05018B4_2_A7Jv_mCherry-C_F_G02_20_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNGCT GCCNGGCGCC TACNACGTCA ACATCAAGTT GGACATCACC
           61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
          121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGATG
          181 GAGCAGCGCC GCGTCACCGA CTTCTTCGCG CGCCGCCGCC CCGGGCCCCC CCGCATCGCG
          241 CCGCCCAAGC TGGCCTGCCG CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGCC
          301 TCCGCTACCA GTGGCAGCCG CAAGCGCGCC CGCCCGCCCG CCGCCCCCGG ACGCGACCAG
          361 GCCAGGCCAC CGGCCCGCGC GGCCGCGCGG CTGTCGGTGG ACGAGGTTTC CAGCCCCAGT
          421 ACCCCCGAGG CCCCAGACAT CCCAGCCTGC CCTTCTCCGG GCCAGAAGAT AAAGAAAACC
          481 GGTCGCGCCA AGCGCGGCAG CGGCGCCACC AACTTCAGCC TGCTGAAGCA GGCCGGCGAC
          541 GTGGAGGAGA ACCCCGGCCC CCGCGAATTC GCCACCATGG CCCTGTCCAA CAAGTTCATC
          601 GGCGACGACA TGAAGATGAC CTACCACATG GACGGCTGCG TGAACGGCCA CTACTTCACC
          661 GTGAAGGGCG AGGGCAGCGG CAAGCCCTAC GAGGGCACCC AGACCTCCAC CTTCAAGGTG
          721 ACCATGGCCA ACGGCGGCCC CCTGGCCTTC TCCTTCGACA TCCTGTCCAC CGTGTTCATG
          781 TACGGCAACC GCTGCTTCAC CGCCNACNNC ACCAGCATGC CCGANTACTT CNAGCANGCC
          841 TTCCCCGANG GCATGTCCTA CGAGAGAANC TTCACCTACG ANGNNGGNGG CGNGGCCNCC
          901 GNCAGCNGGG ANNNCAGCCN GAANGGNAAC TGCTNCNAGC ACAAGTNCNN NTTNCNCGGN
        961 GNNNNNNNNC CNNNNNANNG NCCNNNNNNN GNNNNAGANN ANNNNNNNNN N
        //
        Primer: AmCyan1-H_F
        Region: insert mid (AmCyan1, hCdt1(1_110))
        Sequence file: RDB15453_A7Jvk.seq check
        >15453_15453_B1K5F_2_AmCyan1-H_F_G02_20_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNGG CNNCCNGNNC TCCNCCTTCA AGGTGACCAT GGCCAACGGC
           61 GGCCCCCTGG CCTTCTCCTT CGACATCCTG TCCACCGTGT TCATGTACGG CAACCGCTGC
          121 TTCACCGCCT ACCCCACCAG CATGCCCGAC TACTTCAAGC AGGCCTTCCC CGACGGCATG
          181 TCCTACGAGA GAACCTTCAC CTACGAGGAC GGCGGCGTGG CCACCGCCAG CTGGGAGATC
          241 AGCCTGAAGG GCAACTGCTT CGAGCACAAG TCCACCTTCC ACGGCGTGAA CTTCCCCGCC
          301 GACGGCCCCG TGATGGCCAA GAAGACCACC GGCTGGGACC CCTCCTTCGA GAAGATGACC
          361 GTGTGCGACG GCATCTTGAA GGGCGACGTG ACCGCCTTCC TGATGCTGCA GGGCGGCGGC
          421 AACTACAGAT GCCAGTTCCA CACCTCCTAC AAGACCAAGA AGCCCGTGAC CATGCCCCCC
          481 AACCACGTGG TGGAGCACCG CATCGCCAGA ACCGACCTGG ACAAGGGCGG CAACAGCGTG
          541 CAGCTGACCG AGCACGCCGT GGCCCACATC ACCTCCGTGG TGCCCTTCGG ATATCCATCA
          601 CACTGGCGGC CGCTCGAGAT GANNCCCNGT ATGAAGCANN NNNAGAAGAA ATCAAAGAGA
          661 ATATAAAGAA TAGTTNTGTC CCNNGAANAN NTCTGNNNNN NNNTCNNNNN NNGCNTCTNN
        721 NNNNNNNN
        //
        Primer: HIV-B_R
        Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1
        Sequence file: RDB15453_A7Jvl.seq check
        >15453_15453_B1K5F_2_HIV-B_R_E02_14_ABI24.ab1
            1 NNNNNNNNNC NNNNNANNNT TTTGGCGTAC TCNCCAGTCG CCGCCCCTCG CCTCTTGCCG
           61 TGCGCGCTTC AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT
          121 TCAAGTCCCT GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG
          181 GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG
          241 CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA
          301 GATCTGGTCT AACCAGAGAG ACCCAGTACA GGCAAAACGC GCTGCTTATA TAGACCTCCC
          361 ACCGTACACG CCTACCGCCC ATTTGCGTCA ATGGGGCGGA GTTGTTACGA CATTTTGGAA
          421 AGTCCCGTTG ATTTTGGTGC CAAAACAAAC TCCCATTGAC GTCAATGGGG TGGAGACTTG
          481 GAAATCCCCG TGAGTCAAAC CGCTATCCAC GCCCATTGAT GTACTGCCAA AACCGCATCA
          541 CCATGGTAAT AGCGATGACT AATACGTAGA TGTACTGCCA AGTANGAAAG TCCCATAAGG
          601 TCATGTACTG GGCATAATGC CAGGCGGGCC ATTTACCGTC NTTGACGTCN ATANGGGGCG
        661 TACTTGGNAT ANGANANACT TGANGTACTG NCNNNNGGNN NNNTNNNNNN NNNN
        //
        Primer: BGH_rev2
        Region: 3'LTR, PRE
        Sequence file: RDB15453_A7Jvm.seq check
        >D05018B4_2_A7Jv_BGH_rev2_E01_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNGGNNGNN GGGCNNACAA CAGATGGCTG GCAACTAGAA
           61 GGCACAGTCG AGGCTGNTCN NCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA
          121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC
          181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA
          241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC
          301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC
          361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT
          421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG
          481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT
          541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG
          601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT
          661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA
          721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG
          781 GTCTCGACGG TATCGATGCG GGGANGCGGC CCAAAGGGAN ATCCGACTCG TCTGANGGCG
          841 AANGCGAANA NGCGGAAGAN GCCGCANANC CNGCAGCANG CCNNNGGAAN GNANGGTCCG
          901 CNNGATTGAG GNNCNAANGG ACGTANCANA ANGGACGNNC CNCGCANAAN CCANGNNGNN
        961 NCACAGGNNA NCAGCCNTNN NAANGNNNNN NNCNTNNNCN NNNNNNNNN
        //
        Primer: SV40pro_F
        Region: Zeo, SV40 pA
        Sequence file: RDB15453_A7Jvn.seq check
        >D05018B4_2_A7Jv_SV40pro_F_A03_03_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NANNNNTGAN GANGCTTTTT TGGANGNCTA GGCTTTTGCA
           61 AAAAGCTCCC GGGAGCTTGT ATATCCNTTT TCGGATCTGA TCAGCACGTG TTGACAATTA
          121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
          181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
          241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
          301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
          361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
          421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
          481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
          541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
          601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
          661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
          721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
          781 CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCN ANCTCTAGCT AGAGCTTGGC
          841 GTAATCATGG TCNTAGCTGT TTCCTGTGTG AAATTGNTAT CCGCTCACAA TTCCNCACNA
          901 CATACGAGCC NGAANCATAA AGNGNAAAGN CNGGGGNGNC NAANGANTGA GCNNACTCNC
          961 ATTNATTGCN NNGCGCTCAC NNCCNNNTTT NCANNNNGNN AANNCTNNNN NGNNNNNNGC
        1021 NTNNNNGNNN NNNNCNNNNN NNNNGGNNNN NNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl