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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)2.1/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). CMV promoter.

Catalog number RDB15453
Resource name tFucci(CA)2.1/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of nucleotide sequence LC212777 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA (for use for not-for-profit academic purpose)[Link]
Remarks Remember that you will be working with samples containing infectious virus.
UCSD's MTA is also required. Please visit Additional Forms section.
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs).
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15453 tFucci(CA)2.1/pCSII-CMV DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15453_A7Jvp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R
Sequence file: RDB15453_A7Jva.seq check
>D05018B4_2_A7Jv_mCherry-C_R_D01_10_ABI24.ab1
    1 CTTGTGGGGT AATGTCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT
   61 CTTGGCCTTG TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
  121 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC
  181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG
  241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC
  301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG
  361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA
  421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT
  481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG
  541 GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT
  601 GTTATCCTCC TCGCCCTTGC TCACCATGGT GGATCCTATG CTAGCGGATC TGACGGTTCA
  661 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA
  721 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CAAAACAAAC
  781 TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC
  841 GCCCATTGAT GTACTGCCAA AACCGCATCA CCATGGTAAT AGCGATGACT AATACGTAGA
  901 TGTACTGCCA AGTAGGAAAG TCCCATAAGG TCATGTACTG GGCATAATGC CAAGGCGGGC
  961 CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT
 1021 GCCCAAGGTG GCAGTTACCG TAAAATACTT CACCCATTTG ACGTCAATGG AAAGTCCTAA
1081 TTGCGGTACC TATGGGAAAC AATACGTTCA ATTTATTGG
//
Primer: mCherry-C_F
Sequence file: RDB15453_A7Jvb.seq check
>D05018B4_2_A7Jv_mCherry-C_F_G02_20_ABI24.ab1
    1 TAACAAGTAG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
   61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
  121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGATG
  181 GAGCAGCGCC GCGTCACCGA CTTCTTCGCG CGCCGCCGCC CCGGGCCCCC CCGCATCGCG
  241 CCGCCCAAGC TGGCCTGCCG CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGCC
  301 TCCGCTACCA GTGGCAGCCG CAAGCGCGCC CGCCCGCCCG CCGCCCCCGG ACGCGACCAG
  361 GCCAGGCCAC CGGCCCGCGC GGCCGCGCGG CTGTCGGTGG ACGAGGTTTC CAGCCCCAGT
  421 ACCCCCGAGG CCCCAGACAT CCCAGCCTGC CCTTCTCCGG GCCAGAAGAT AAAGAAAACC
  481 GGTCGCGCCA AGCGCGGCAG CGGCGCCACC AACTTCAGCC TGCTGAAGCA GGCCGGCGAC
  541 GTGGAGGAGA ACCCCGGCCC CCGCGAATTC GCCACCATGG CCCTGTCCAA CAAGTTCATC
  601 GGCGACGACA TGAAGATGAC CTACCACATG GACGGCTGCG TGAACGGCCA CTACTTCACC
  661 GTGAAGGGCG AGGGCAGCGG CAAGCCCTAC GAGGGCACCC AGACCTCCAC CTTCAAGGTG
  721 ACCATGGCCA ACGGCGGCCC CCTGGCCTTC TCCTTCGACA TCCTGTCCAC CGTGTTCATG
  781 TACGGCAACC GCTGCTTCAC CGCCTACCCC ACCAGCATGC CCGACTACTT CAAGCAGGCC
  841 TTCCCCGACG GCATGTCCTA CGAGAGAACC TTCACCTACG AGGACGGCGG CGTGGCCACC
  901 GCCAGCTGGG AGATCAGCCT GAAGGGCAAC TGCTTCGAGC ACAAGTCCAC CTTCCACGGC
  961 GTGAACTTCC CCGCCGACGG CCCCGTGATG GGCCAAGAAG ACCACCGGCT GGGACCCCTC
 1021 CTTTCGAGAG ATGACCGTGT GCGACGGCAT CTTGAGGCCG ACGTGACGCT TCTGAATGCT
 1081 GCATGCCGCC GCCACTACAG ATGCCAGTCA CACTTCTTAC AGACAGGAGG CCGTGACCAT
1141 GCCCCCCC
//
Primer: PRE_rev
Sequence file: RDB15453_A7Jvc.seq check
>D05018B4_2_A7Jv_PRE_rev_E02_14_ABI24.ab1
    1 ACGGTCACAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA
  121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA
  181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG
  241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT
  301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT
  361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT
  421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCGA
  481 AGGGCACCAC GGAGGTGATG TGGGCCACGG CGTGCTCGGT CAGCTGCACG CTGTTGCCGC
  541 CCTTGTCCAG GTCGGTTCTG GCGATGCGGT GCTCCACCAC GTGGTTGGGG GGCATGGTCA
  601 CGGGCTTCTT GGTCTTGTAG GAGGTGTGGA ACTGGCATCT GTAGTTGCCG CCGCCCTGCA
  661 GCATCAGGAA GGCGGTCACG TCGCCCTTCA AGATGCCGTC GCACACGGTC ATCTTCTCGA
  721 AGGAGGGGTC CCAGCCGGTG GTCTTCTTGG CCATCACGGG GCCGTCGGCG GGGAAGTTCA
  781 CGCCGTGGAA GGTGGACTTG TGCTCGAAGC AGTTGCCCTT CAGCTGATCT CCCAGCTGGC
  841 GGTGGCCACG CCGCCGTCCT CGTAGTGAAG GTTCTCTCGT AGACATGCCG TCGGGGAAGG
  901 CCTGCTTGAA GTAGTCGGGC ATGCTGGTGG GGTAGGCGGT GAAGCAGCGG GTTGCCGTAC
  961 ATGAACACGG TGGACAGGAT GTCGAAGAGA CGCCAGGGGG CCGCCGTTGG CCATGGTCAC
 1021 CTTGATGTGA GTCTGGGTGC CCTCGTAGGC TGGCGGCTGC CCTCGCCCTT CACGTGAGTA
 1081 ATTGATCGAT CACGCAGCCG TCCATGTGTA AGGTCAATCT CATGTCGTCG CCGAATGAAC
1141 CTTGTTTGGG GACAG
//
Primer: HIVgp_R1
Sequence file: RDB15453_A7Jvd.seq check
>D05018B4_2_A7Jv_HIVgp_R1_F02_17_ABI24.ab1
    1 CAGGCTCTAT TCCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG
   61 GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT
  121 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGGCCGCTT GCTGTGCGGT
  181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT
  241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC
  301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA
  361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT
  421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA
  481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC
  541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC
  601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC
  661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
  721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
  781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
  841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC
  901 ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA CGACATTTTG GAAAGTCCCG
  961 TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG GGGTGGAGAC TTGGAAAATC
 1021 CCCGTGAGTC AAACGCTATC CACGCCCATT GATGTACTGC AAACGCATCA CATGGTAATA
 1081 GCGATGACTA TACGTAGATG TACTGCCAAG TAGAAAGTCC CATACGGTTC ATGTTACTTG
1141 GCATAATTGC CAGGCCGGGT CCAATTTAAC CGCGTCTCAT G
//
Primer: BGH_rev2
Sequence file: RDB15453_A7Jve.seq check
>D05018B4_2_A7Jv_BGH_rev2_E01_13_ABI24.ab1
    1 CAAAACCAGG GGTCAGGAAG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA
  121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC
  181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA
  241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC
  301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC
  361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT
  421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG
  481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT
  541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG
  601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT
  661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA
  721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG
  781 GTCTCGACGG TATCGATGCG GGGAGGCGGC CCAAAGGGAG ATCCGACTCG TCTGAGGGCG
  841 AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC CGGCAGCAGG CCGCGGGAAG GAAAGGTCCG
  901 CTGGATTGAG GGCCGAAGGG ACGTAGCAGA AAGGACGTCC CGCGCAGAAT CCAGGTGGCA
  961 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTTCCCC GACAACACCA CGGGAATTGT
 1021 CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC AGCGGGCAAG GCAGGCCGGC CGATGAGTTC
1081 GCCGTGCATA GAAGGAAGCG AAGGTCCCGG AAAGAAGCTG ACACAGGTT
//
Primer: SV40pro_F
Sequence file: RDB15453_A7Jvf.seq check
>D05018B4_2_A7Jv_SV40pro_F_A03_03_ABI24.ab1
    1 CCCTGGTCCT GGAGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
  241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
  301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
  361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
  421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
  481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
  541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
  661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
  781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
  841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AAACCTGTCG TGCCAGCTGC
 1021 ATTAATGAAT CGGCCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGCGC TCTCGCTTCT
 1081 CGCTCACTGA CTCGCTGCGC TCGGGTCGGT CGGCTGCCGC GAGCCGTATC AGCTTCACTC
1141 CAAAGGCGGT TAATTACCGG TTATTTCCCA CAGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl