Resource data sheet
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mVenus/pRSETB

Venus fluorescent protein. Bacterial expression.

Catalog number RDB15117
Resource name mVenus/pRSETB
Clone info. Venus fluorescent protein. Bacterial expression.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15117 mVenus/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15117_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15117_A7H9a.seq
>D04498A2_A7H9_1_T7_20bp_C01_07_ABI24.ab1
    1 AAAATTCCGG CCTAGGAATA TGTTTGGTTT AACTTTAAGA AGGAGGATAT ACATATGCGG
   61 GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA CTGGTGGACA GCAAATGGGT
  121 CGGGATCTGT ACGACGATGA CGATAAGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC
  181 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  241 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  301 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  361 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  421 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  481 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  541 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  601 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
  661 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
  721 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
  781 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  841 ACCGCCGCCG GGATCACTCT CGGCATGGGA CGAGCTGTAC AAGTAACTCG AGAAGCTTTG
  901 ATCCGGGCTG CTAACCAAAG CCCGAAAGGA AGCTGAGTTG GGCTGCTGCC ACGGCTGAGC
  961 AAATAAACTA GGCATAACCC CTTTGGGGGC CTCTAAACGG GTCTTGGAGG GGGTTTTTTT
 1021 GGCTGGAAAG GAGGAACTTA TAATCCGGGA TCCTGGCGTA AATAGCGAAA GAAGGGCCCC
 1081 GGCTACCGAA TCGGCCCTTT CCCCAACAGT TTGCGCCAGG CTTGAAATTG GCGAAATGGG
 1141 ACGCCGCCCT GTAGCCGGCG CCATTAAGCG CGGCCGGGTG GTGTGTGGTT ACCGCACAAC
1201 GTGAGTAACC GACTCCTACT
//
Primer: f1ori_R3 RDB15117_A7H9b.seq
>D04498A2_A7H9_1_f1ori_R3_A12_01_ABI08.ab1
    1 TTTTCTCGTC CTTTCGGCTA CCACTGTTTG GGAGGCGATC GGTGCGGGCC TCTTCGCTAT
   61 TACGCCAGAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA CCCGTTTAGA
  121 GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT CAGCTTCCTT
  181 TCGGGCTTTG TTAGCAGCCG GATCAAGCTT CTCGAGTTAC TTGTACAGCT CGTCCATGCC
  241 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT
  301 GGGGTCTTTG CTCAGCTTGG ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG
  361 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC CGCCGTCCTC
  421 GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT CGGCGGTGAT
  481 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC
  541 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC
  601 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA
  661 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGGC
  721 GAAGCACTGC AGGCCGTAGC CCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT
  781 GCCGGTGGTG CAAGATCAGC TTCAGGGTCA GCTTGCCCGT AGGTGGGCAT CGCCCTCGCC
  841 CTCGCCGGAC ACGCTGAAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTTC GACCAGGATG
  901 GGCACCACCC CCGGGTGAAC AGCTCCTCGC CCTTTGCTCA CCATGGTGGG CGAACCCGGG
  961 TGGAATCCTT TATCGTCATC GGTCGGTACA GAATCCCCGG ACCCCATTTT GCATGTCCAA
1021 CCAGTCAATG CTTAGCCCAT ACCAATGGAT GGATTGAATG AATGGAGTGA GAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.
reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.
reference Tsugama, D., Arabidopsis heterotrimeric G protein beta subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52. Biochim. Biophys. Acta 1823 (12): 2254-2260 (2012). PMID 23058975.

2019.07.30

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