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mVenus/pRSETB (#RDB15117)

Venus fluorescent protein. Bacterial expression.

Clone info. Venus fluorescent protein. Bacterial expression.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15117hts01.seq checkAssembled from experimentally sequenced data.
Publication Nagai, T., Nat. Biotechnol. 20 (1): 87-90 (2002). [link to RRC of NBRP]
Test sheet RDB15117_B0Chp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, an acknowledgement to Dr. Miyawaki is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, an acknowledgement to Dr. Miyawaki is requested.
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15117 mVenus/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The mVenus/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15117).

Reference section:

Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K., Miyawaki, A., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]
reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.
reference Tsugama, D., Arabidopsis heterotrimeric G protein beta subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52. Biochim. Biophys. Acta 1823 (12): 2254-2260 (2012). PMID 23058975.