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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mVenus/pRSETB

Venus fluorescent protein. Bacterial expression.

Catalog number RDB15117
Resource name mVenus/pRSETB
Clone info. Venus fluorescent protein. Bacterial expression.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15117 mVenus/pRSETB DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15117_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp
            Region: 6xHis, T7 tag, X press tag, insert 5'
            Sequence file: RDB15117_A7H9a.seq check
            >D04498A2_A7H9_1_T7_20bp_C01_07_ABI24.ab1
                1 AAAATTCCGG CCTAGGAATA TGTTTGGTTT AACTTTAAGA AGGAGGATAT ACATATGCGG
               61 GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA CTGGTGGACA GCAAATGGGT
              121 CGGGATCTGT ACGACGATGA CGATAAGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC
              181 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
              241 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
              301 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
              361 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
              421 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
              481 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
              541 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
              601 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
              661 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
              721 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
              781 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
              841 ACCGCCGCCG GGATCACTCT CGGCATGGGA CGAGCTGTAC AAGTAACTCG AGAAGCTTTG
              901 ATCCGGGCTG CTAACCAAAG CCCGAAAGGA AGCTGAGTTG GGCTGCTGCC ACGGCTGAGC
              961 AAATAAACTA GGCATAACCC CTTTGGGGGC CTCTAAACGG GTCTTGGAGG GGGTTTTTTT
             1021 GGCTGGAAAG GAGGAACTTA TAATCCGGGA TCCTGGCGTA AATAGCGAAA GAAGGGCCCC
             1081 GGCTACCGAA TCGGCCCTTT CCCCAACAGT TTGCGCCAGG CTTGAAATTG GCGAAATGGG
             1141 ACGCCGCCCT GTAGCCGGCG CCATTAAGCG CGGCCGGGTG GTGTGTGGTT ACCGCACAAC
            1201 GTGAGTAACC GACTCCTACT
            //
            Primer: f1ori_R3
            Region: T7 Ter, insert 3'
            Sequence file: RDB15117_A7H9b.seq check
            >D04498A2_A7H9_1_f1ori_R3_A12_01_ABI08.ab1
                1 TTTTCTCGTC CTTTCGGCTA CCACTGTTTG GGAGGCGATC GGTGCGGGCC TCTTCGCTAT
               61 TACGCCAGAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA CCCGTTTAGA
              121 GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT CAGCTTCCTT
              181 TCGGGCTTTG TTAGCAGCCG GATCAAGCTT CTCGAGTTAC TTGTACAGCT CGTCCATGCC
              241 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT
              301 GGGGTCTTTG CTCAGCTTGG ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG
              361 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC CGCCGTCCTC
              421 GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT CGGCGGTGAT
              481 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC
              541 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC
              601 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA
              661 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGGC
              721 GAAGCACTGC AGGCCGTAGC CCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT
              781 GCCGGTGGTG CAAGATCAGC TTCAGGGTCA GCTTGCCCGT AGGTGGGCAT CGCCCTCGCC
              841 CTCGCCGGAC ACGCTGAAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTTC GACCAGGATG
              901 GGCACCACCC CCGGGTGAAC AGCTCCTCGC CCTTTGCTCA CCATGGTGGG CGAACCCGGG
              961 TGGAATCCTT TATCGTCATC GGTCGGTACA GAATCCCCGG ACCCCATTTT GCATGTCCAA
            1021 CCAGTCAATG CTTAGCCCAT ACCAATGGAT GGATTGAATG AATGGAGTGA GAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.
            reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.
            reference Tsugama, D., Arabidopsis heterotrimeric G protein beta subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52. Biochim. Biophys. Acta 1823 (12): 2254-2260 (2012). PMID 23058975.

            2022.02.09

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