Resource data sheet
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Venus/pCS2

Venus fluorescent protein. Mammalian expression.

Catalog number RDB15116
Resource name Venus/pCS2
Alternative name USE10C/pCS2
Clone info. Venus fluorescent protein. Mammalian expression.
Variant of EYFP having substitution at F46L, F64L, V68L, M153T, V163A and S175G.
Vector backbone pCS2 (plasmid)
Size of vector backbone 4.1 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15116 Venus/pCS2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15116_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F RDB15116_A7H9a.seq
>D04498A1_A7H9_1_pCMV-IE94_F_A01_01_ABI08.ab1
    1 CTGGTGCCCG GGAGGGGCGG GGCCTAAATG GGGAGGTCTA TATAAGCAAT GCTCGTTTAG
   61 GGAACCGCCA TTCTGCCTGG GGACGTCGGA GCAAGCTTGA TTTAGGTGAC ACTATAGAAT
  121 ACAAGCTACT TGTTCTTTTT GCAGGATCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
  181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
  241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA
  301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC
  361 AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
  421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
  481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
  541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA
  601 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC
  661 ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG
  721 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC GCCCTGAGCA
  781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA
  841 TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCAA GGCCTCTCGA GCCTCTAGAA
  901 CTATAGTGAG TCGTATTACG TAGATCCAGA CATGGATAAA GATACATTTG ATGAGTTTGG
  961 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTAAT TTGTGAAATT TGTGATGCTA
 1021 TTGCTTTATT TGTAACCCAT TATAAGCTGC AATAAAACAA GTTAACAACC AACAATTTGC
 1081 ATTCATTTTT ATGGTTTCAG GGTTCAGGGG AGGTGTGGAA GTTTTTTAAA TTCGCGCCGC
1141 GCGCCAATGC AATTGGGCCC GTACCCCAGC CTTTTTTGTT TTTCCCTATT TAGAGGTGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

2019.02.26

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