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Venus/pCS2

Venus fluorescent protein. Mammalian expression.

Catalog number RDB15116
Resource name Venus/pCS2
Alternative name USE10C/pCS2
Clone info. Venus fluorescent protein. Mammalian expression.
Comment Variant of EYFP having substitution at F46L, F64L, V68L, M153T, V163A and S175G.
Vector backbone pCS2 (plasmid)
Size of vector backbone 4.1 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank
External Database
Aequorea victoria GFP
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
            Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
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            提供条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
            Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
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            提供同意書 (MTA, 非営利学術目的用)[Word]
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。

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            RDB15116 Venus/pCS2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The Venus/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15116).

            Reference section:

            Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K., Miyawaki, A., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15116_A7H9p1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCMV-IE94_F (Pr0506)
            Region: SP6 pro,insert 5'
            Sequence file: RDB15116_A7H9b.seq check
            >D04498A1_A7H9_1_pCMV-IE94_F_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GNNAGGNNNN NNTAAGNNAT GCTCGTTTAG
               61 GGAACCGNCN NTCTGCCTGG GGACGTCGGA GCAAGCTTGA TTTAGGTGAC ACTATAGAAT
              121 ACAAGCTACT TGTTCTTTTT GCAGGATCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
              181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
              241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA
              301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC
              361 AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
              421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
              481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
              541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA
              601 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC
              661 ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CNNNNNGAAC ACCCCCATCG
              721 NCGACGGCCC CGNGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC GCCCTGAGCA
              781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGN NTTCGTGANC GCCGCCGGGA
              841 TCACTCTCGG CATGGACNAG CTGTACAAGT AAGAANNNNN NGCCTCTCNA NCCTCTNNAA
              901 CTATANTGAG TCGNNNNANG TAGATCNNGA CNTNNNNNAN NNNACNTTNG ATGAGTTTGG
              961 ACAANNCNCN ACNNNAATGN NGNGAAAAAA ANGCTTTNNT TTGNGAANNT TGNGANGNTN
            1021 TNGCTTTATT TGTAANCNAN NNNNAGCNNC NNNNAAANNA NTNAACAANN NNNNNNNN
            //
            Primer: mVenus-M_F (Pr0574)
            Region: insert 3',bGH pA
            Sequence file: RDB15116_A7H9c.seq check
            >15116_15116_B3DjF_mVenus-M_F_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNATCN NGGCNNACTT NNAGATCCGC CACAACATCG AGGACGGCGG
               61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
              121 GCCCGACAAC CACTACCTGA GCTACCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
              181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
              241 GCTGTACAAG TAAGAATTCA AGGCCTCTCG AGCCTCTAGA ACTATAGTGA GTCGTATTAC
              301 GTAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG
              361 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
              421 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
              481 GAGGTGTGGG AGGTTTTTTA ATTCGCGGCC GCGGCGCCAA TGCATTGGGC CCGGTACCCA
              541 GCTTTTGTTC CCTTTAGTGA GGGTTAATTG CGCGCTTGNC GTAATCATGG TCATAGCTGT
              601 TTCCTGTGTG AAATTGTTAT CCGCTCNCAA TTCNNCNCAA CATACNANCC GGGANCNTAA
              661 AGTGNAAAGN CTGGNNGNCT NANGNNTGAN CTNNCTNNNN TTNATNGNNT NGNNNTNNNN
            721 NNCNNNNTNC NNNNNNNANN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]

            2023.11.23

            GNP_filter3_RDBDEP_html_231027.pl