Please review the QC test results indicated by icon below as well as clone information before placing your order.

Venus/pCS2

Venus fluorescent protein. Mammalian expression.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15116_A7H9p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F (Pr0506) Region: SP6 pro,insert 5' Sequence file: RDB15116_A7H9b.seq
>D04498A1_A7H9_1_pCMV-IE94_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GNNAGGNNNN NNTAAGNNAT GCTCGTTTAG    61 GGAACCGNCN NTCTGCCTGG GGACGTCGGA GCAAGCTTGA TTTAGGTGAC ACTATAGAAT   121 ACAAGCTACT TGTTCTTTTT GCAGGATCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA   181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG   241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA   301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC   361 AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC   421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC   481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG   541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA   601 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC   661 ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CNNNNNGAAC ACCCCCATCG   721 NCGACGGCCC CGNGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC GCCCTGAGCA   781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGN NTTCGTGANC GCCGCCGGGA   841 TCACTCTCGG CATGGACNAG CTGTACAAGT AAGAANNNNN NGCCTCTCNA NCCTCTNNAA   901 CTATANTGAG TCGNNNNANG TAGATCNNGA CNTNNNNNAN NNNACNTTNG ATGAGTTTGG   961 ACAANNCNCN ACNNNAATGN NGNGAAAAAA ANGCTTTNNT TTGNGAANNT TGNGANGNTN 1021 TNGCTTTATT TGTAANCNAN NNNNAGCNNC NNNNAAANNA NTNAACAANN NNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert 3',bGH pA Sequence file: RDB15116_A7H9c.seq
>15116_15116_B3DjF_mVenus-M_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNATCN NGGCNNACTT NNAGATCCGC CACAACATCG AGGACGGCGG    61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT   121 GCCCGACAAC CACTACCTGA GCTACCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG   181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA   241 GCTGTACAAG TAAGAATTCA AGGCCTCTCG AGCCTCTAGA ACTATAGTGA GTCGTATTAC   301 GTAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG   361 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA   421 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG   481 GAGGTGTGGG AGGTTTTTTA ATTCGCGGCC GCGGCGCCAA TGCATTGGGC CCGGTACCCA   541 GCTTTTGTTC CCTTTAGTGA GGGTTAATTG CGCGCTTGNC GTAATCATGG TCATAGCTGT   601 TTCCTGTGTG AAATTGTTAT CCGCTCNCAA TTCNNCNCAA CATACNANCC GGGANCNTAA   661 AGTGNAAAGN CTGGNNGNCT NANGNNTGAN CTNNCTNNNN TTNATNGNNT NGNNNTNNNN 721 NNCNNNNTNC NNNNNNNANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl