Venus fluorescent protein. Mammalian expression.
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External Database Aequorea victoria GFP Reference sequence |
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Terms and conditions for distribution | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Approval form [Word] ![]() |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
提供条件 | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 宮脇敦史博士による提供承諾書 [Word ![]() |
備考 | 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15116 | Venus/pCS2 | DNA solution |
Materials & Methods section:
The Venus/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15116). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Fluorescent protein resources (English text) |
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Featured content | Fluorescent protein resources (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15116_A7H9p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pCMV-IE94_F (Pr0506) Region: SP6 pro,insert 5' Sequence file: RDB15116_A7H9b.seq ![]() |
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>D04498A1_A7H9_1_pCMV-IE94_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GNNAGGNNNN NNTAAGNNAT GCTCGTTTAG 61 GGAACCGNCN NTCTGCCTGG GGACGTCGGA GCAAGCTTGA TTTAGGTGAC ACTATAGAAT 121 ACAAGCTACT TGTTCTTTTT GCAGGATCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA 181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG 241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA 301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC 361 AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC 421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC 481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG 541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA 601 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC 661 ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CNNNNNGAAC ACCCCCATCG 721 NCGACGGCCC CGNGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC GCCCTGAGCA 781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGN NTTCGTGANC GCCGCCGGGA 841 TCACTCTCGG CATGGACNAG CTGTACAAGT AAGAANNNNN NGCCTCTCNA NCCTCTNNAA 901 CTATANTGAG TCGNNNNANG TAGATCNNGA CNTNNNNNAN NNNACNTTNG ATGAGTTTGG 961 ACAANNCNCN ACNNNAATGN NGNGAAAAAA ANGCTTTNNT TTGNGAANNT TGNGANGNTN 1021 TNGCTTTATT TGTAANCNAN NNNNAGCNNC NNNNAAANNA NTNAACAANN NNNNNNNN // |
Primer: mVenus-M_F (Pr0574) Region: insert 3',bGH pA Sequence file: RDB15116_A7H9c.seq ![]() |
>15116_15116_B3DjF_mVenus-M_F_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNATCN NGGCNNACTT NNAGATCCGC CACAACATCG AGGACGGCGG 61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT 121 GCCCGACAAC CACTACCTGA GCTACCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG 181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA 241 GCTGTACAAG TAAGAATTCA AGGCCTCTCG AGCCTCTAGA ACTATAGTGA GTCGTATTAC 301 GTAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG 361 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA 421 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG 481 GAGGTGTGGG AGGTTTTTTA ATTCGCGGCC GCGGCGCCAA TGCATTGGGC CCGGTACCCA 541 GCTTTTGTTC CCTTTAGTGA GGGTTAATTG CGCGCTTGNC GTAATCATGG TCATAGCTGT 601 TTCCTGTGTG AAATTGTTAT CCGCTCNCAA TTCNNCNCAA CATACNANCC GGGANCNTAA 661 AGTGNAAAGN CTGGNNGNCT NANGNNTGAN CTNNCTNNNN TTNATNGNNT NGNNNTNNNN 721 NNCNNNNTNC NNNNNNNANN NNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. |
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2023.05.22
GNP_filter3_RDBDEP_html_230512.pl