Venus fluorescent protein. Mammalian expression.
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Alternative name | USE10C/pCS2 |
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Clone info. | Venus fluorescent protein. Mammalian expression. |
Comment | Variant of EYFP having substitution at F46L, F64L, V68L, M153T, V163A and S175G. |
Vector backbone | pCS2 (plasmid) |
Size of vector backbone | 4.1 kb |
Selectable markers | Amp^r |
Growth conditions | LB + Amp, 37oC |
Gene/insert name | Aequorea victoria GFP cDNA |
Depositor|Developer | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002). |
備考 | 提供条件は英文のまま記載してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15116 | Venus/pCS2 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The Venus/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15116). |
Reference section:
Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K., Miyawaki, A., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP] |
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