RDB15469 - pET28/Dronpa-theta-D4

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pET28/Dronpa-theta-D4 (#RDB15469)

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.

Clone info.	Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.
Vector backbone	Plasmid
Gene/insert name	Clostridium perfringens perfringolysin O (pfoA)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15469	pET28/Dronpa-theta-D4	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET28/Dronpa-theta-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15469).

Reference section:

Mizuno, H., Abe, M., Dedecker, P., ARocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15469_A7Jqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2 () Sequence file: RDB15469_A7Jqa.seq
>D04498J6_A7Jq_1_1_PBR-BamHI-R2_A04_01_ABI24.ab1 1 GTGCGTGGCA GCCATTCGAC GTGAGCGGCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT 61 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC 121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC CATGGGCAGC AGCCATCATC 181 ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCGG TACCATGAGT GTGATTAAAC 241 CAGACATGAA GATCAAGCTG CGTATGGAAG GCGCTGTAAA TGGACACCCG TTCGCGATTG 301 AAGGAGTTGG CCTTGGGAAG CCTTTCGAGG GAAAACAGAG TATGGACCTT AAAGTCAAAG 361 AAGGCGGACC TCTGCCTTTC GCCTATGACA TCTTGACAAC TGTGTTCTGT TACGGCAACA 421 GGGTATTCGC CAAATACCCA GAAAATATAG TAGACTATTT CAAGCAGTCG TTTCCTGAGG 481 GCTACTCTTG GGAACGAAGC ATGAATTACG AAGACGGGGG CATTTGTAAC GCGACAAACG 541 ACATAACCCT GGATGGTGAC TGTTATATCT ATGAAATTCG ATTTGATGGT GTGAACTTTC 601 CTGCCAATGG TCCAGTTATG CAGAAGAGGA CTGTGAAATG GGAGCCATCC ACTGAGAAAT 661 TGTATGTGCG TGATGGAGTG CTGAAGGGTG ATGTTAACAT GGCTCTGTCG CTTGAAGGAG 721 GTGGCCATTA CCGATGTGAC TTCAAAACTA CTTATAAAGC TAAGAAGGTT GTCCAGTTGC 781 CAGACTATCA CTTTGTGGAC CACCACATTG AGATTAAAAG CCACGACAAA GATTACAGTA 841 ATGTTAATCT GCATGAGCAT GCCGAAGCGC ATTCTGAGCT GCCGAGGCAG GCCAAGAAGC 901 TTAAGGGAAA AATAAACTTA GATCATAGTG GAGCCTATGT TGCACAGTTT GAAGTAGCCT 961 GGGATGAAGT TTCATATGAC AAAGAAGGAA ATGAAGTTTT AACTCATAAA ACATGGGATG 1021 GGAAATTATC AAGATAAAAC AGCTTCACTA TTCAACAGTA AATACCTCTT GAGGCTAATG 1081 CAAGAAATAT AGATTAAAAG CCAGAGAGTG TACAGGGTCC TTGCTTGGGC AATTGGCTGG 1141 GAAGAAGAAT GGTTACTA //
Primer: T7 terminator () Sequence file: RDB15469_A7Jqb.seq
>D04498J6_A7Jq_1_1_T7 terminator_C04_07_ABI24.ab1 1 AAATTGGCTT TCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGC 61 TCGAGTTAAT TGTAAGTAAT ACTAGATCCA GGGTATAAAG TTGTTCCCCA TATTGAAACA 121 TTTATATTAT TTGTTAATGG AACATCATAT TCACTTATAA CATCTCTCCA CCATTCCCAA 181 GCAAGGCCTG TACACTCTCT TGCTTTTATT CTTATATTTC TTGCATTAGC TTCAAGAGGT 241 ATTACTGTTG AATAGTGAGC TGTTTTATCT TGATAATTTC CATCCCATGT TTTATGAGTT 301 AAAACTTCAT TTCCTTCTTT GTCATATGAA ACTTCATCCC AGGCTACTTC AAACTGTGCA 361 ACATAGGCTC CACTATGATC TAAGTTTATT TTTCCCTTAA GCTTCTTGGC CTGCCTCGGC 421 AGCTCAGAAT GCGCTTCGGC ATGCTCATGC AGATTAACAT TACTGTAATC TTTGTCGTGG 481 CTTTTAATCT CAATGTGGTG GTCCACAAAG TGATAGTCTG GCAACTGGAC AACCTTCTTA 541 GCTTTATAAG TAGTTTTGAA GTCACATCGG TAATGGCCAC CTCCTTCAAG CGACAGAGCC 601 ATGTTAACAT CACCCTTCAG CACTCCATCA CGCACATACA ATTTCTCAGT GGATGGCTCC 661 CATTTCACAG TCCTCTTCTG CATAACTGGA CCATTGGCAG GAAAGTTCAC ACCATCAAAT 721 CGAATTTCAT AGATATAACA GTCACCATCC AGGGTTATGT CGTTTGTCGC GTTACAAATG 781 CCCCCGTCTT CGTAATTCAT GCTTCGTTCC CAAGAGTAGC CCTCAGGAAA CGACTGCTTG 841 AAATAGTCTA CTATATTTTC TGGGTATTTG GCGAATACCC TGTTGCCGTA ACAGAACACA 901 GTTGTCAAGA TGTCATAGGC GAAAGGCAGA GGTCCGCCTT CTTTGACTTT AAGGTCCATA 961 CTCTGTTTTC CCTCGAAAGG CTTCCCAAGG CCAACTCCTT CAATCGCGAA CGGGTGTCCA 1021 TTTACAGCGC CTTCCATACG CAGCTTGATC TTCATGTCTG GGTTTTAATC ACACTCATGT 1081 ACGCTGGCCG CGCGGCACCA GGCCGCTGCT GTGAATGATG AATGATGGAT TGACTGCCTG 1141 GCCAATGGGT ATAATCCTTC CCATTCCTA //
Primer: lacIpro_F () Sequence file: RDB15469_A7Jqc.seq
>D04498J6-1_A7Jq_lacpro_F_C09_09_ABI24.ab1 1 GGTAGCATGA TGCGCGGAGA GAGTCATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT 61 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG 121 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT 181 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG 241 CCACATCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG 301 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT 361 GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC 421 CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT 481 TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA 541 CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG 601 GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC 661 GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC 721 AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG 781 ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG 841 ATATCTCGGT AGTGGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA 901 ACCACCATCA AACAGGATTT TTCGCCTGCT GGGGCAAAAC CAGCGTGGAC CGCTTGCTGC 961 AACTCTCTCA GGGCCAGGCC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGCTGGAA 1021 AGAAACCACC CTGGCGCCCA ATACGCCAAC CGCCTCTCCC GCGCGGTTGT GATCCATAAT 1081 TGCAGCCTGG CCACGAACAA GGTTTTCGCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]

Resource search

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Other resources in our bank

Clostridium perfringens perfringolysin O (pfoA) and ortholog

External Database
Clostridium perfringens perfringolysin O (pfoA)

in KEGG Orthology

Reference sequence