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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28/Dronpa-theta-D4 (#RDB15469)

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.

Clone info. Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.
Vector backbone Plasmid
Gene/insert name Clostridium perfringens perfringolysin O (pfoA)
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Mizuno, H. et al., Chem. Sci. 2: 1548-1553 (2011).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Mizuno, H. et al., Chem. Sci. 2: 1548-1553 (2011).
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15469 pET28/Dronpa-theta-D4 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET28/Dronpa-theta-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15469).

Reference section:

Mizuno, H., Abe, M., Dedecker, P., ARocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15469_A7Jqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2 ()
Sequence file: RDB15469_A7Jqa.seq check
>D04498J6_A7Jq_1_1_PBR-BamHI-R2_A04_01_ABI24.ab1
    1 GTGCGTGGCA GCCATTCGAC GTGAGCGGCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT
   61 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
  121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC CATGGGCAGC AGCCATCATC
  181 ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCGG TACCATGAGT GTGATTAAAC
  241 CAGACATGAA GATCAAGCTG CGTATGGAAG GCGCTGTAAA TGGACACCCG TTCGCGATTG
  301 AAGGAGTTGG CCTTGGGAAG CCTTTCGAGG GAAAACAGAG TATGGACCTT AAAGTCAAAG
  361 AAGGCGGACC TCTGCCTTTC GCCTATGACA TCTTGACAAC TGTGTTCTGT TACGGCAACA
  421 GGGTATTCGC CAAATACCCA GAAAATATAG TAGACTATTT CAAGCAGTCG TTTCCTGAGG
  481 GCTACTCTTG GGAACGAAGC ATGAATTACG AAGACGGGGG CATTTGTAAC GCGACAAACG
  541 ACATAACCCT GGATGGTGAC TGTTATATCT ATGAAATTCG ATTTGATGGT GTGAACTTTC
  601 CTGCCAATGG TCCAGTTATG CAGAAGAGGA CTGTGAAATG GGAGCCATCC ACTGAGAAAT
  661 TGTATGTGCG TGATGGAGTG CTGAAGGGTG ATGTTAACAT GGCTCTGTCG CTTGAAGGAG
  721 GTGGCCATTA CCGATGTGAC TTCAAAACTA CTTATAAAGC TAAGAAGGTT GTCCAGTTGC
  781 CAGACTATCA CTTTGTGGAC CACCACATTG AGATTAAAAG CCACGACAAA GATTACAGTA
  841 ATGTTAATCT GCATGAGCAT GCCGAAGCGC ATTCTGAGCT GCCGAGGCAG GCCAAGAAGC
  901 TTAAGGGAAA AATAAACTTA GATCATAGTG GAGCCTATGT TGCACAGTTT GAAGTAGCCT
  961 GGGATGAAGT TTCATATGAC AAAGAAGGAA ATGAAGTTTT AACTCATAAA ACATGGGATG
 1021 GGAAATTATC AAGATAAAAC AGCTTCACTA TTCAACAGTA AATACCTCTT GAGGCTAATG
 1081 CAAGAAATAT AGATTAAAAG CCAGAGAGTG TACAGGGTCC TTGCTTGGGC AATTGGCTGG
1141 GAAGAAGAAT GGTTACTA
//
Primer: T7 terminator ()
Sequence file: RDB15469_A7Jqb.seq check
>D04498J6_A7Jq_1_1_T7 terminator_C04_07_ABI24.ab1
    1 AAATTGGCTT TCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGC
   61 TCGAGTTAAT TGTAAGTAAT ACTAGATCCA GGGTATAAAG TTGTTCCCCA TATTGAAACA
  121 TTTATATTAT TTGTTAATGG AACATCATAT TCACTTATAA CATCTCTCCA CCATTCCCAA
  181 GCAAGGCCTG TACACTCTCT TGCTTTTATT CTTATATTTC TTGCATTAGC TTCAAGAGGT
  241 ATTACTGTTG AATAGTGAGC TGTTTTATCT TGATAATTTC CATCCCATGT TTTATGAGTT
  301 AAAACTTCAT TTCCTTCTTT GTCATATGAA ACTTCATCCC AGGCTACTTC AAACTGTGCA
  361 ACATAGGCTC CACTATGATC TAAGTTTATT TTTCCCTTAA GCTTCTTGGC CTGCCTCGGC
  421 AGCTCAGAAT GCGCTTCGGC ATGCTCATGC AGATTAACAT TACTGTAATC TTTGTCGTGG
  481 CTTTTAATCT CAATGTGGTG GTCCACAAAG TGATAGTCTG GCAACTGGAC AACCTTCTTA
  541 GCTTTATAAG TAGTTTTGAA GTCACATCGG TAATGGCCAC CTCCTTCAAG CGACAGAGCC
  601 ATGTTAACAT CACCCTTCAG CACTCCATCA CGCACATACA ATTTCTCAGT GGATGGCTCC
  661 CATTTCACAG TCCTCTTCTG CATAACTGGA CCATTGGCAG GAAAGTTCAC ACCATCAAAT
  721 CGAATTTCAT AGATATAACA GTCACCATCC AGGGTTATGT CGTTTGTCGC GTTACAAATG
  781 CCCCCGTCTT CGTAATTCAT GCTTCGTTCC CAAGAGTAGC CCTCAGGAAA CGACTGCTTG
  841 AAATAGTCTA CTATATTTTC TGGGTATTTG GCGAATACCC TGTTGCCGTA ACAGAACACA
  901 GTTGTCAAGA TGTCATAGGC GAAAGGCAGA GGTCCGCCTT CTTTGACTTT AAGGTCCATA
  961 CTCTGTTTTC CCTCGAAAGG CTTCCCAAGG CCAACTCCTT CAATCGCGAA CGGGTGTCCA
 1021 TTTACAGCGC CTTCCATACG CAGCTTGATC TTCATGTCTG GGTTTTAATC ACACTCATGT
 1081 ACGCTGGCCG CGCGGCACCA GGCCGCTGCT GTGAATGATG AATGATGGAT TGACTGCCTG
1141 GCCAATGGGT ATAATCCTTC CCATTCCTA
//
Primer: lacIpro_F ()
Sequence file: RDB15469_A7Jqc.seq check
>D04498J6-1_A7Jq_lacpro_F_C09_09_ABI24.ab1
    1 GGTAGCATGA TGCGCGGAGA GAGTCATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT
   61 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG
  121 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT
  181 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG
  241 CCACATCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG
  301 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT
  361 GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC
  421 CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT
  481 TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA
  541 CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG
  601 GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC
  661 GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC
  721 AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG
  781 ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG
  841 ATATCTCGGT AGTGGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA
  901 ACCACCATCA AACAGGATTT TTCGCCTGCT GGGGCAAAAC CAGCGTGGAC CGCTTGCTGC
  961 AACTCTCTCA GGGCCAGGCC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGCTGGAA
 1021 AGAAACCACC CTGGCGCCCA ATACGCCAAC CGCCTCTCCC GCGCGGTTGT GATCCATAAT
1081 TGCAGCCTGG CCACGAACAA GGTTTTCGCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]