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pET28/Dronpa-theta-D4

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.

Catalog number RDB15469
Resource name pET28/Dronpa-theta-D4
Clone info. Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.
Vector backbone Plasmid
Gene/insert name Clostridium perfringens perfringolysin O (pfoA)
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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RDB15469 pET28/Dronpa-theta-D4 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011).

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15469_A7Jqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2 RDB15469_A7Jqa.seq
>D04498J6_A7Jq_1_1_PBR-BamHI-R2_A04_01_ABI24.ab1
    1 GTGCGTGGCA GCCATTCGAC GTGAGCGGCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT
   61 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
  121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC CATGGGCAGC AGCCATCATC
  181 ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCGG TACCATGAGT GTGATTAAAC
  241 CAGACATGAA GATCAAGCTG CGTATGGAAG GCGCTGTAAA TGGACACCCG TTCGCGATTG
  301 AAGGAGTTGG CCTTGGGAAG CCTTTCGAGG GAAAACAGAG TATGGACCTT AAAGTCAAAG
  361 AAGGCGGACC TCTGCCTTTC GCCTATGACA TCTTGACAAC TGTGTTCTGT TACGGCAACA
  421 GGGTATTCGC CAAATACCCA GAAAATATAG TAGACTATTT CAAGCAGTCG TTTCCTGAGG
  481 GCTACTCTTG GGAACGAAGC ATGAATTACG AAGACGGGGG CATTTGTAAC GCGACAAACG
  541 ACATAACCCT GGATGGTGAC TGTTATATCT ATGAAATTCG ATTTGATGGT GTGAACTTTC
  601 CTGCCAATGG TCCAGTTATG CAGAAGAGGA CTGTGAAATG GGAGCCATCC ACTGAGAAAT
  661 TGTATGTGCG TGATGGAGTG CTGAAGGGTG ATGTTAACAT GGCTCTGTCG CTTGAAGGAG
  721 GTGGCCATTA CCGATGTGAC TTCAAAACTA CTTATAAAGC TAAGAAGGTT GTCCAGTTGC
  781 CAGACTATCA CTTTGTGGAC CACCACATTG AGATTAAAAG CCACGACAAA GATTACAGTA
  841 ATGTTAATCT GCATGAGCAT GCCGAAGCGC ATTCTGAGCT GCCGAGGCAG GCCAAGAAGC
  901 TTAAGGGAAA AATAAACTTA GATCATAGTG GAGCCTATGT TGCACAGTTT GAAGTAGCCT
  961 GGGATGAAGT TTCATATGAC AAAGAAGGAA ATGAAGTTTT AACTCATAAA ACATGGGATG
 1021 GGAAATTATC AAGATAAAAC AGCTTCACTA TTCAACAGTA AATACCTCTT GAGGCTAATG
 1081 CAAGAAATAT AGATTAAAAG CCAGAGAGTG TACAGGGTCC TTGCTTGGGC AATTGGCTGG
1141 GAAGAAGAAT GGTTACTA
//
Primer: T7 terminator RDB15469_A7Jqb.seq
>D04498J6_A7Jq_1_1_T7 terminator_C04_07_ABI24.ab1
    1 AAATTGGCTT TCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGC
   61 TCGAGTTAAT TGTAAGTAAT ACTAGATCCA GGGTATAAAG TTGTTCCCCA TATTGAAACA
  121 TTTATATTAT TTGTTAATGG AACATCATAT TCACTTATAA CATCTCTCCA CCATTCCCAA
  181 GCAAGGCCTG TACACTCTCT TGCTTTTATT CTTATATTTC TTGCATTAGC TTCAAGAGGT
  241 ATTACTGTTG AATAGTGAGC TGTTTTATCT TGATAATTTC CATCCCATGT TTTATGAGTT
  301 AAAACTTCAT TTCCTTCTTT GTCATATGAA ACTTCATCCC AGGCTACTTC AAACTGTGCA
  361 ACATAGGCTC CACTATGATC TAAGTTTATT TTTCCCTTAA GCTTCTTGGC CTGCCTCGGC
  421 AGCTCAGAAT GCGCTTCGGC ATGCTCATGC AGATTAACAT TACTGTAATC TTTGTCGTGG
  481 CTTTTAATCT CAATGTGGTG GTCCACAAAG TGATAGTCTG GCAACTGGAC AACCTTCTTA
  541 GCTTTATAAG TAGTTTTGAA GTCACATCGG TAATGGCCAC CTCCTTCAAG CGACAGAGCC
  601 ATGTTAACAT CACCCTTCAG CACTCCATCA CGCACATACA ATTTCTCAGT GGATGGCTCC
  661 CATTTCACAG TCCTCTTCTG CATAACTGGA CCATTGGCAG GAAAGTTCAC ACCATCAAAT
  721 CGAATTTCAT AGATATAACA GTCACCATCC AGGGTTATGT CGTTTGTCGC GTTACAAATG
  781 CCCCCGTCTT CGTAATTCAT GCTTCGTTCC CAAGAGTAGC CCTCAGGAAA CGACTGCTTG
  841 AAATAGTCTA CTATATTTTC TGGGTATTTG GCGAATACCC TGTTGCCGTA ACAGAACACA
  901 GTTGTCAAGA TGTCATAGGC GAAAGGCAGA GGTCCGCCTT CTTTGACTTT AAGGTCCATA
  961 CTCTGTTTTC CCTCGAAAGG CTTCCCAAGG CCAACTCCTT CAATCGCGAA CGGGTGTCCA
 1021 TTTACAGCGC CTTCCATACG CAGCTTGATC TTCATGTCTG GGTTTTAATC ACACTCATGT
 1081 ACGCTGGCCG CGCGGCACCA GGCCGCTGCT GTGAATGATG AATGATGGAT TGACTGCCTG
1141 GCCAATGGGT ATAATCCTTC CCATTCCTA
//
Primer: lacIpro_F RDB15469_A7Jqc.seq
>D04498J6-1_A7Jq_lacpro_F_C09_09_ABI24.ab1
    1 GGTAGCATGA TGCGCGGAGA GAGTCATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT
   61 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG
  121 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT
  181 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG
  241 CCACATCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG
  301 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT
  361 GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC
  421 CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT
  481 TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA
  541 CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG
  601 GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC
  661 GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC
  721 AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG
  781 ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG
  841 ATATCTCGGT AGTGGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA
  901 ACCACCATCA AACAGGATTT TTCGCCTGCT GGGGCAAAAC CAGCGTGGAC CGCTTGCTGC
  961 AACTCTCTCA GGGCCAGGCC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGCTGGAA
 1021 AGAAACCACC CTGGCGCCCA ATACGCCAAC CGCCTCTCCC GCGCGGTTGT GATCCATAAT
1081 TGCAGCCTGG CCACGAACAA GGTTTTCGCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011).

2019.07.30

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