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SCAT3.1/pcDNA4 HMB (#RDB15705)

Expression vector of FRET based apoptosis probe.

Clone info. Expression vector of FRET based caspase3 activity monitoring probe. Two fluorescent proteins ECFP and Venus were connected by a caspase3 substrate peptide DEVD (Asp-Glu-Val-Asp).
Vector backbone pcDNA4/HisMax B (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Growth conditions 37oC, LB + Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15705hts01.seq checkAssembled from experimentally sequenced data.
Publication Nagai, T., Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444. [link to RRC of NBRP]
Test sheet RDB15705_B3Ilp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T., Miyawaki, A., Biochem. Biophys. Res. Commun. 319: 72-77 (2004).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T., Miyawaki, A., Biochem. Biophys. Res. Commun. 319: 72-77 (2004).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15705 SCAT3.1/pcDNA4 HMB DNA solution

How to cite this biological resource

Materials & Methods section:

The SCAT3.1/pcDNA4 HMB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15705).

Reference section:

Nagai, T., Miyawaki, A., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444. [link to RRC of NBRP]
reference Kominami, K., In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. PLoS One 7 (11): e50218 (2012). PMID 23185580. [link to Journal]
reference Tomura, M., Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions. Int. Immunol. 21 (10): 1145-1150 (2009). PMID 19710208. [link to Journal]