RDB15705 - SCAT3.1/pcDNA4 HMB

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

SCAT3.1/pcDNA4 HMB (#RDB15705)

Expression vector of FRET based apoptosis probe.

Clone info.	Expression vector of FRET based caspase3 activity monitoring probe. Two fluorescent proteins ECFP and Venus were connected by a caspase3 substrate peptide DEVD (Asp-Glu-Val-Asp).
Vector backbone	pcDNA4/HisMax B (Plasmid)
Size of vector backbone	5.2 kb
Selectable markers	Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Sequence (partial)	RDB15705pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T., Miyawaki, A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Miyawaki, A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Miyawaki, A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15705	SCAT3.1/pcDNA4 HMB	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The SCAT3.1/pcDNA4 HMB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15705).

Reference section:

Nagai, T., Miyawaki, A., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15705_A7Jqp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, QBI SP163, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15705_A7Jqa.seq
>15705_15705_A9GhF_2_CMV-Forward_A11_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNTGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTAGCG CAGAGGCTTG 181 GGGCAGCCGA GCGGCAGCCA GGCCCCGGCC CGGGCCTCGG TTCCAGAAGG GAGAGGAGCC 241 CGCCAAGGCG CGCAAGAGAG CGGGCTGCCT CGCAGTCCGA GCCGGAGAGG GAGCGCGAGC 301 CGCGCCGGCC CCGGACGGCC TCCGAAACCA TGGGGGGTTC TCATCATCAT CATCATCATG 361 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA 421 AGGTACCTAA GGATCCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA 481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAGGTT CAGCGTGTCC GGCGAGGGCG 541 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 601 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTGGGG CGTGCAGTGC TTCAGCCGCT 661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA NGCTACGTCC 721 AGGAGCGTAC CATCTTCTTC AAGGACGACN GCAACTACAA GACCCGCGCC GAGGTGAAGT 781 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAANGG CATCGACTTC AANGAGGANG 841 GNAACATCCT GGGGCACAAG CTGGAGTACA ACTACNTCAG CCNCNACGTC NATATCACCG 901 NCNANNNGCA GAAGANCNNN ATCNNNGGNC NACTTCNAGA TNCGCCACNN NATCGAGGAN 961 NNNAGCGTGC AGCTCGNCNA NCACTNNCAG NNNNNNNCCC CCNTCNGNNN NNNNCCCNNN 1021 NNGNNNCCNN NNANNNNNNN CNNNNNNNNN N //
Primer: BGH_rev2 (Pr0606) Region: bGHp(A), insert3' Sequence file: RDB15705_A7Jqb.seq
>15705_15705_A9GhF_2_BGH_rev2_B11_02_ABI08.ab1 1 NNNNNNNNNN NNNNNANNNN NNNNNGNNGG GNAAACNANN GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG 121 TGCTGGATAT CTGCAGAATT CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC 181 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG 241 GGCGGACTGG TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG 301 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT 361 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT 421 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC 481 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT 541 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC 601 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC 661 GTAGCCCANG GTGGTCACGA GGGTGGGCCA NGGCACGGGC AGCTTGCCGG TGGTGCAGAT 721 CAGCTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA 781 CTTGTGGNCG TTTACNNTCG CCGTCCAGCT CGACCAGNAT GGGCNNCNNC CCCNGTGAAC 841 AGCTCCTCNC CCTTGCTCAC CANNGTACCA TCNACCTCAT CANTGNTCCN NGCGGNNNGT 901 CACNAACTCC ANCNNNGNNN NTNTGATNNC GCNTTCTCGT NNGGGNNTTT NNTCNNGGCN 961 GANNGGGNNG CTCAGGNNGT GGTTGNNNGG GCANCANCNC NGGGNNCCNN CNCCNNNTGG 1021 GGGNNNNNNN NCNTNGNNAN NNGNNNNNNN NNN //
Primer: pTrcHis-F (Pr0094) Region: insert mid Sequence file: RDB15705_A7Jqc.seq
>15705_15705_A9GhF_2_pTrcHis-F_C11_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNNNATNN NGATNAGGTA CCTAAGGNNC CCNTGGTGAG 61 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT 121 AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT 181 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC 241 CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA 301 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT TCTTCAAGGA 361 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG 421 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA 481 GTACAACTAC ATCAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA 541 GGCCCACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA 601 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG 661 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA 721 GTTCGTGACC GCCGCCGGGA TCACTGATGA NGTCGATGGT NCCATGGTGA GCNNGGGCGA 781 GGAGCTGTNN NNNNGGGTGG TGCCNNTCCT GGTCGANCTG GACNNNGACG TAAACNGNCN 841 CAAGTTCAGC GTGTCCNGNG AGGGCGAGGG CGANGCNNCC TACNGNNAGC TGANCCTGNA 901 GCTGATCTGC NNCACCNGNA AGCTGNCNNN GCCCTGGNCC ANCNNCGTGN NNACCNTGGG 961 CTNNNNCCTG CANTGCTTCG CCCNNNNNCC NNANNNNNNN NANNCANNNN NNNNNN //
Primer: HismaxMCS_R (Pr0823) Region: insert mid Sequence file: RDB15705_A7Jqd.seq
>15705_15705_B0BkF_HismaxMCS_R_H09_24_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTGNNCCC GGCGGCGGTC NCGAACTCCN GCAGGNCCAT 61 GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC TGGTAGCTCA GGTAGTGGTT 121 GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG 181 CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTGGCCTTGA TGCCGTTCTT 241 CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC 301 CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT 361 GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT 421 GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT 481 GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC AGGGTGGTCA CGAGGGTGGG 541 CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC AGGGTCAGCT TGCCGTAGGT 601 GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG 661 CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTACC 721 ATCGACCTCA TCAGTGATCC CGGCGGCGGT CACGAACTCC AGCANGANCA TGTGNTCGCG 781 CTTCTCGTTG GGGTCTTTGC TCANGGCGGA NTGNNTGCTC ANGTANTGGT TGTCGGGCAG 841 CANCACGGGG CCGTCGCCNA TGGNGGTGTT CTGNTGGNAN TGNTCGGCGA NCTGCNCGCN 901 GCNNNCCNCN ATGTTGTGGC GGATCNNNNA GTGGGCCTNN NTGNCNNTCN NCNGNTNGNN 961 GGCGNNGNNA TANNNGNTNN NGGNNNNNNN NNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro, Zeo Sequence file: RDB15705_A7Jqe.seq
>15705_15705_A9GhF_2_SV40pro_F_D11_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NGGNNGNTTT TTTGGAGGNC TAGGCTTTTG 61 CAAAAAGCTC CCGGGAGCTT GTATATCNNT TTTCGGATCT GATCAGCACG TGTTGACAAT 121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG 181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG 241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG 301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC 361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC 421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG 481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG 541 GAGCAGGACT GNNNNNTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG 601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG 661 CTGGAGTTCT TCGNNNNNNC CAACTTGTTT ATTGCAGCTT ATANNGGTTA CAAAATNAAG 721 CAANTAGCAT CACAAATTTC NCNNNTAAAG CATTTTTTTC ACTGNNNNCT AGTTTGTGGN 781 TTGTCCAAAN TNNNCNNNGT NTCNTNATCA TGTCTGTATA CCGNCNACCT CNNNNCTNNA 841 NNNNGGNNNN NNCNTGGNCN NNGCTGNTTC NTGNGTGAAA ATTGNTNNTN NNNNTNNNNN 901 TNNNNNNNNN ANNNANNNAG CCCGGAAGCN NNNNNNNNAN ANNNNCNGGG GGGNNNNNNN 961 NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444. [link to RRC of NBRP]
reference	Kominami, K., In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. PLoS One 7 (11): e50218 (2012). PMID 23185580.
reference	Tomura, M., Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions. Int. Immunol. 21 (10): 1145-1150 (2009). PMID 19710208.

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Aequorea victoria GFP

Reference sequence