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SCAT3.1/pcDNA4 HMB

Expression vector of FRET based apoptosis probe.

Catalog number RDB15705
Resource name SCAT3.1/pcDNA4 HMB
Clone info. Expression vector of FRET based caspase3 activity monitoring probe. Two fluorescent proteins ECFP and Venus were connected by a caspase3 substrate peptide DEVD (Asp-Glu-Val-Asp).
Vector backbone pcDNA4/HisMax B (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T., Miyawaki, A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Schedule A (GFP-A) [Link]
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Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15705 SCAT3.1/pcDNA4 HMB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sensor and Visualization (English text)
Featured content Apoptosis (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15705_A7Jqp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, QBI SP163, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15705_A7Jqa.seq check
>15705_15705_A9GhF_2_CMV-Forward_A11_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTAGCG CAGAGGCTTG
  181 GGGCAGCCGA GCGGCAGCCA GGCCCCGGCC CGGGCCTCGG TTCCAGAAGG GAGAGGAGCC
  241 CGCCAAGGCG CGCAAGAGAG CGGGCTGCCT CGCAGTCCGA GCCGGAGAGG GAGCGCGAGC
  301 CGCGCCGGCC CCGGACGGCC TCCGAAACCA TGGGGGGTTC TCATCATCAT CATCATCATG
  361 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
  421 AGGTACCTAA GGATCCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
  481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAGGTT CAGCGTGTCC GGCGAGGGCG
  541 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
  601 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTGGGG CGTGCAGTGC TTCAGCCGCT
  661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA NGCTACGTCC
  721 AGGAGCGTAC CATCTTCTTC AAGGACGACN GCAACTACAA GACCCGCGCC GAGGTGAAGT
  781 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAANGG CATCGACTTC AANGAGGANG
  841 GNAACATCCT GGGGCACAAG CTGGAGTACA ACTACNTCAG CCNCNACGTC NATATCACCG
  901 NCNANNNGCA GAAGANCNNN ATCNNNGGNC NACTTCNAGA TNCGCCACNN NATCGAGGAN
  961 NNNAGCGTGC AGCTCGNCNA NCACTNNCAG NNNNNNNCCC CCNTCNGNNN NNNNCCCNNN
1021 NNGNNNCCNN NNANNNNNNN CNNNNNNNNN N
//
Primer: BGH_rev2
Region: bGHp(A), insert3'
Sequence file: RDB15705_A7Jqb.seq check
>15705_15705_A9GhF_2_BGH_rev2_B11_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNANNNN NNNNNGNNGG GNAAACNANN GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG
  121 TGCTGGATAT CTGCAGAATT CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
  181 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
  241 GGCGGACTGG TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
  301 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT
  361 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT
  421 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
  481 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
  541 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
  601 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC
  661 GTAGCCCANG GTGGTCACGA GGGTGGGCCA NGGCACGGGC AGCTTGCCGG TGGTGCAGAT
  721 CAGCTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
  781 CTTGTGGNCG TTTACNNTCG CCGTCCAGCT CGACCAGNAT GGGCNNCNNC CCCNGTGAAC
  841 AGCTCCTCNC CCTTGCTCAC CANNGTACCA TCNACCTCAT CANTGNTCCN NGCGGNNNGT
  901 CACNAACTCC ANCNNNGNNN NTNTGATNNC GCNTTCTCGT NNGGGNNTTT NNTCNNGGCN
  961 GANNGGGNNG CTCAGGNNGT GGTTGNNNGG GCANCANCNC NGGGNNCCNN CNCCNNNTGG
1021 GGGNNNNNNN NCNTNGNNAN NNGNNNNNNN NNN
//
Primer: pTrcHis-F
Region: insert mid
Sequence file: RDB15705_A7Jqc.seq check
>15705_15705_A9GhF_2_pTrcHis-F_C11_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNNATNN NGATNAGGTA CCTAAGGNNC CCNTGGTGAG
   61 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  121 AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  181 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  241 CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  301 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT TCTTCAAGGA
  361 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  421 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  481 GTACAACTAC ATCAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA
  541 GGCCCACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  601 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
  661 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  721 GTTCGTGACC GCCGCCGGGA TCACTGATGA NGTCGATGGT NCCATGGTGA GCNNGGGCGA
  781 GGAGCTGTNN NNNNGGGTGG TGCCNNTCCT GGTCGANCTG GACNNNGACG TAAACNGNCN
  841 CAAGTTCAGC GTGTCCNGNG AGGGCGAGGG CGANGCNNCC TACNGNNAGC TGANCCTGNA
  901 GCTGATCTGC NNCACCNGNA AGCTGNCNNN GCCCTGGNCC ANCNNCGTGN NNACCNTGGG
961 CTNNNNCCTG CANTGCTTCG CCCNNNNNCC NNANNNNNNN NANNCANNNN NNNNNN
//
Primer: HismaxMCS_R
Region: insert mid
Sequence file: RDB15705_A7Jqd.seq check
>15705_15705_B0BkF_HismaxMCS_R_H09_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTGNNCCC GGCGGCGGTC NCGAACTCCN GCAGGNCCAT
   61 GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC TGGTAGCTCA GGTAGTGGTT
  121 GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG
  181 CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTGGCCTTGA TGCCGTTCTT
  241 CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC
  301 CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT
  361 GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT
  421 GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT
  481 GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC AGGGTGGTCA CGAGGGTGGG
  541 CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC AGGGTCAGCT TGCCGTAGGT
  601 GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG
  661 CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTACC
  721 ATCGACCTCA TCAGTGATCC CGGCGGCGGT CACGAACTCC AGCANGANCA TGTGNTCGCG
  781 CTTCTCGTTG GGGTCTTTGC TCANGGCGGA NTGNNTGCTC ANGTANTGGT TGTCGGGCAG
  841 CANCACGGGG CCGTCGCCNA TGGNGGTGTT CTGNTGGNAN TGNTCGGCGA NCTGCNCGCN
  901 GCNNNCCNCN ATGTTGTGGC GGATCNNNNA GTGGGCCTNN NTGNCNNTCN NCNGNTNGNN
961 GGCGNNGNNA TANNNGNTNN NGGNNNNNNN NNN
//
Primer: SV40pro_F
Region: EM7 pro, Zeo
Sequence file: RDB15705_A7Jqe.seq check
>15705_15705_A9GhF_2_SV40pro_F_D11_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NGGNNGNTTT TTTGGAGGNC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCNNT TTTCGGATCT GATCAGCACG TGTTGACAAT
  121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
  181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
  241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
  301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
  361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
  421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
  481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
  541 GAGCAGGACT GNNNNNTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
  601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
  661 CTGGAGTTCT TCGNNNNNNC CAACTTGTTT ATTGCAGCTT ATANNGGTTA CAAAATNAAG
  721 CAANTAGCAT CACAAATTTC NCNNNTAAAG CATTTTTTTC ACTGNNNNCT AGTTTGTGGN
  781 TTGTCCAAAN TNNNCNNNGT NTCNTNATCA TGTCTGTATA CCGNCNACCT CNNNNCTNNA
  841 NNNNGGNNNN NNCNTGGNCN NNGCTGNTTC NTGNGTGAAA ATTGNTNNTN NNNNTNNNNN
  901 TNNNNNNNNN ANNNANNNAG CCCGGAAGCN NNNNNNNNAN ANNNNCNGGG GGGNNNNNNN
961 NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444.
reference Kominami, K., In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. PLoS One 7 (11): e50218 (2012). PMID 23185580.
reference Tomura, M., Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions. Int. Immunol. 21 (10): 1145-1150 (2009). PMID 19710208.

2020.10.02

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