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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

SCAT3.1/pcDNA4 HMB

Expression vector of FRET based apoptosis probe.

Catalog number RDB15705
Resource name SCAT3.1/pcDNA4 HMB
Clone info. Expression vector of FRET based caspase3 activity monitoring probe. Two fluorescent proteins ECFP and Venus were connected by a caspase3 substrate peptide DEVD (Asp-Glu-Val-Asp).
Vector backbone pcDNA4/HisMax B (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (partial) RDB15705pka01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Nagai, T., Miyawaki, A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Nagai, T., Miyawaki, A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15705 SCAT3.1/pcDNA4 HMB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Sensor and Visualization (English text)
            Featured content Apoptosis (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15705_A7Jqp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro, QBI SP163, 6xHis, T7 tag, Xpress tag, insert 5'
            Sequence file: RDB15705_A7Jqa.seq check
            >15705_15705_A9GhF_2_CMV-Forward_A11_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNTGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
              121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTAGCG CAGAGGCTTG
              181 GGGCAGCCGA GCGGCAGCCA GGCCCCGGCC CGGGCCTCGG TTCCAGAAGG GAGAGGAGCC
              241 CGCCAAGGCG CGCAAGAGAG CGGGCTGCCT CGCAGTCCGA GCCGGAGAGG GAGCGCGAGC
              301 CGCGCCGGCC CCGGACGGCC TCCGAAACCA TGGGGGGTTC TCATCATCAT CATCATCATG
              361 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
              421 AGGTACCTAA GGATCCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
              481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAGGTT CAGCGTGTCC GGCGAGGGCG
              541 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
              601 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTGGGG CGTGCAGTGC TTCAGCCGCT
              661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA NGCTACGTCC
              721 AGGAGCGTAC CATCTTCTTC AAGGACGACN GCAACTACAA GACCCGCGCC GAGGTGAAGT
              781 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAANGG CATCGACTTC AANGAGGANG
              841 GNAACATCCT GGGGCACAAG CTGGAGTACA ACTACNTCAG CCNCNACGTC NATATCACCG
              901 NCNANNNGCA GAAGANCNNN ATCNNNGGNC NACTTCNAGA TNCGCCACNN NATCGAGGAN
              961 NNNAGCGTGC AGCTCGNCNA NCACTNNCAG NNNNNNNCCC CCNTCNGNNN NNNNCCCNNN
            1021 NNGNNNCCNN NNANNNNNNN CNNNNNNNNN N
            //
            Primer: BGH_rev2
            Region: bGHp(A), insert3'
            Sequence file: RDB15705_A7Jqb.seq check
            >15705_15705_A9GhF_2_BGH_rev2_B11_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNANNNN NNNNNGNNGG GNAAACNANN GATGGCTGGC AACTAGAAGG
               61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG
              121 TGCTGGATAT CTGCAGAATT CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
              181 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
              241 GGCGGACTGG TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
              301 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT
              361 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT
              421 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
              481 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
              541 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
              601 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC
              661 GTAGCCCANG GTGGTCACGA GGGTGGGCCA NGGCACGGGC AGCTTGCCGG TGGTGCAGAT
              721 CAGCTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
              781 CTTGTGGNCG TTTACNNTCG CCGTCCAGCT CGACCAGNAT GGGCNNCNNC CCCNGTGAAC
              841 AGCTCCTCNC CCTTGCTCAC CANNGTACCA TCNACCTCAT CANTGNTCCN NGCGGNNNGT
              901 CACNAACTCC ANCNNNGNNN NTNTGATNNC GCNTTCTCGT NNGGGNNTTT NNTCNNGGCN
              961 GANNGGGNNG CTCAGGNNGT GGTTGNNNGG GCANCANCNC NGGGNNCCNN CNCCNNNTGG
            1021 GGGNNNNNNN NCNTNGNNAN NNGNNNNNNN NNN
            //
            Primer: pTrcHis-F
            Region: insert mid
            Sequence file: RDB15705_A7Jqc.seq check
            >15705_15705_A9GhF_2_pTrcHis-F_C11_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGNNNATNN NGATNAGGTA CCTAAGGNNC CCNTGGTGAG
               61 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
              121 AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
              181 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
              241 CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
              301 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT TCTTCAAGGA
              361 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
              421 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
              481 GTACAACTAC ATCAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA
              541 GGCCCACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
              601 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
              661 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
              721 GTTCGTGACC GCCGCCGGGA TCACTGATGA NGTCGATGGT NCCATGGTGA GCNNGGGCGA
              781 GGAGCTGTNN NNNNGGGTGG TGCCNNTCCT GGTCGANCTG GACNNNGACG TAAACNGNCN
              841 CAAGTTCAGC GTGTCCNGNG AGGGCGAGGG CGANGCNNCC TACNGNNAGC TGANCCTGNA
              901 GCTGATCTGC NNCACCNGNA AGCTGNCNNN GCCCTGGNCC ANCNNCGTGN NNACCNTGGG
            961 CTNNNNCCTG CANTGCTTCG CCCNNNNNCC NNANNNNNNN NANNCANNNN NNNNNN
            //
            Primer: HismaxMCS_R
            Region: insert mid
            Sequence file: RDB15705_A7Jqd.seq check
            >15705_15705_B0BkF_HismaxMCS_R_H09_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTGNNCCC GGCGGCGGTC NCGAACTCCN GCAGGNCCAT
               61 GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC TGGTAGCTCA GGTAGTGGTT
              121 GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG
              181 CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTGGCCTTGA TGCCGTTCTT
              241 CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC
              301 CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT
              361 GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT
              421 GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT
              481 GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC AGGGTGGTCA CGAGGGTGGG
              541 CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC AGGGTCAGCT TGCCGTAGGT
              601 GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG
              661 CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTACC
              721 ATCGACCTCA TCAGTGATCC CGGCGGCGGT CACGAACTCC AGCANGANCA TGTGNTCGCG
              781 CTTCTCGTTG GGGTCTTTGC TCANGGCGGA NTGNNTGCTC ANGTANTGGT TGTCGGGCAG
              841 CANCACGGGG CCGTCGCCNA TGGNGGTGTT CTGNTGGNAN TGNTCGGCGA NCTGCNCGCN
              901 GCNNNCCNCN ATGTTGTGGC GGATCNNNNA GTGGGCCTNN NTGNCNNTCN NCNGNTNGNN
            961 GGCGNNGNNA TANNNGNTNN NGGNNNNNNN NNN
            //
            Primer: SV40pro_F
            Region: EM7 pro, Zeo
            Sequence file: RDB15705_A7Jqe.seq check
            >15705_15705_A9GhF_2_SV40pro_F_D11_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NGGNNGNTTT TTTGGAGGNC TAGGCTTTTG
               61 CAAAAAGCTC CCGGGAGCTT GTATATCNNT TTTCGGATCT GATCAGCACG TGTTGACAAT
              121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
              181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
              241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
              301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
              361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
              421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
              481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
              541 GAGCAGGACT GNNNNNTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
              601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
              661 CTGGAGTTCT TCGNNNNNNC CAACTTGTTT ATTGCAGCTT ATANNGGTTA CAAAATNAAG
              721 CAANTAGCAT CACAAATTTC NCNNNTAAAG CATTTTTTTC ACTGNNNNCT AGTTTGTGGN
              781 TTGTCCAAAN TNNNCNNNGT NTCNTNATCA TGTCTGTATA CCGNCNACCT CNNNNCTNNA
              841 NNNNGGNNNN NNCNTGGNCN NNGCTGNTTC NTGNGTGAAA ATTGNTNNTN NNNNTNNNNN
              901 TNNNNNNNNN ANNNANNNAG CCCGGAAGCN NNNNNNNNAN ANNNNCNGGG GGGNNNNNNN
            961 NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444.
            reference Kominami, K., In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. PLoS One 7 (11): e50218 (2012). PMID 23185580.
            reference Tomura, M., Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions. Int. Immunol. 21 (10): 1145-1150 (2009). PMID 19710208.

            2022.07.27

            GNP_filter3_RDBDEP_html_220727.pl