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SCAT3.1/pcDNA4 HMB

Expression vector of FRET based apoptosis probe.

Catalog number RDB15705
Resource name SCAT3.1/pcDNA4 HMB
Clone info. Expression vector of FRET based caspase3 activity monitoring probe. Two fluorescent proteins ECFP and Venus were connected by a caspase3 substrate peptide DEVD (Asp-Glu-Val-Asp).
Vector backbone pcDNA4/HisMax B (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T., Miyawaki, A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
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Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15705 SCAT3.1/pcDNA4 HMB Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Apoptosis (English text)
Featured content Sensor and Visualization (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Nagai, T., A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PMID 15158444.
reference Kominami, K., In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. PLoS One 7 (11): e50218 (2012). PMID 23185580.
reference Tomura, M., Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions. Int. Immunol. 21 (10): 1145-1150 (2009). PMID 19710208.

2019.07.17

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