Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmAG1/pRSETB (#RDB15230)

Plasmid clone of fluorescent protein monomeric Azami-Green (mAG).

Clone info. Plasmid clone of fluorescent protein monomeric Azami-Green (mAG) derived from Galaxea fascicularis.
Comment This construct contains a minor variation, which might not be a problem, G to C nucleotide substitution and Glu to Asp amino acid change corresponding to 927nt of LC08717.1 FRB-mAG1. Refer nucleotide sequences AB107915.1 and AB108447.1 as references, too.
Vector backbone pRSETB (Plasmid)
Gene/insert name Galaxea fascicularis GFPAzami-Green cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., J. Biol. Chem. 278: 34167-34171 (2003).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., J. Biol. Chem. 278: 34167-34171 (2003).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15230 hmAG1/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hmAG1/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15230).

Reference section:

Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15230_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp ()
Sequence file: RDB15230_A7H9a.seq check
>D04498D6_1_A7H9_T7_20bp_B01_02_ABI08.ab1
    1 GAAAATTCCC TTTTAAAAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGCGG
   61 GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA CTGGTGGACA GCAAATGGGT
  121 CGGGATCTGT ACGACGATGA CGATAAGGAT CCCACCATGG TGAGCGTGAT CAAGCCCGAG
  181 ATGAAGATCA AGCTGTGCAT GAGGGGCACC GTGAACGGCC ACAACTTCGT GATCGAGGGC
  241 GAGGGCAAGG GCAACCCCTA CGAGGGCACC CAGATCCTGG ACCTGAACGT GACCGAGGGC
  301 GCCCCCCTGC CCTTCGCCTA CGACATCCTG ACCACCGTGT TCCAGTACGG CAACAGGGCC
  361 TTCACCAAGT ACCCCGCCGA CATCCAGGAC TACTTCAAGC AGACCTTCCC CGAGGGCTAC
  421 CACTGGGAGA GGAGCATGAC CTACGAGGAC CAGGGCATCT GCACCGCCAC CAGCAACATC
  481 AGCATGAGGG GCGACTGCTT CTTCTACGAC ATCAGGTTCG ACGGCACCAA CTTCCCCCCC
  541 AACGGCCCCG TGATGCAGAA GAAGACCCTG AAGTGGGAGC CCAGCACCGA GAAGATGTAC
  601 GTGGAGGACG GCGTGCTGAA GGGCGACGTG AACATGAGGC TGCTGCTGGA GGGCGGCGGC
  661 CACTACAGGT GCGACTTCAA GACCACCTAC AAGGCCAAGA AGGACGTGAG GCTGCCCGAC
  721 GCCCACAAGA TCGACCACAG GATCGAGATC CTGAAGCACG ACAAGGACTA CAACAAGGTG
  781 AAGCTGTACG AGAACGCCGT GGCCAGGTAC TCCATGCTGC CCAGCCAGGC CAAGTGAGAA
  841 TTCGAAGCTT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC
  901 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT
  961 TGCTGAAAGG AGGAACTATA TCCGGGATCT GGCGTAATAG CGAAGAGGGC CCGCACCGAT
 1021 CGCTCTTTCC CAACAGTTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGGC
 1081 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTTG ACGGCTAACA CTGGCCAGCG
1141 TCTTAGCGCC CGGCTCGGTT CGCTATTCCG TCCGATCCTT TTCTTCGCCC ACGCGAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]