Resource data sheet
Prev.

hmAG1/pRSETB

Plasmid clone of fluorescent protein monomeric Azami-Green (mAG).

Catalog number RDB15230
Resource name hmAG1/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Azami-Green (mAG) derived from Galaxea fascicularis.
This construct contains a minor variation, which might not be a problem, G to C nucleotide substitution and Glu to Asp amino acid change corresponding to 927nt of LC08717.1 FRB-mAG1.
Vector backbone pRSETB (Plasmid)
Gene/insert name Galaxea fascicularis GFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A. (2003) A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278: 34167-34171.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15230 hmAG1/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15230_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15230_A7H9a.seq
>D04498D6_1_A7H9_T7_20bp_B01_02_ABI08.ab1
    1 GAAAATTCCC TTTTAAAAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGCGG
   61 GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA CTGGTGGACA GCAAATGGGT
  121 CGGGATCTGT ACGACGATGA CGATAAGGAT CCCACCATGG TGAGCGTGAT CAAGCCCGAG
  181 ATGAAGATCA AGCTGTGCAT GAGGGGCACC GTGAACGGCC ACAACTTCGT GATCGAGGGC
  241 GAGGGCAAGG GCAACCCCTA CGAGGGCACC CAGATCCTGG ACCTGAACGT GACCGAGGGC
  301 GCCCCCCTGC CCTTCGCCTA CGACATCCTG ACCACCGTGT TCCAGTACGG CAACAGGGCC
  361 TTCACCAAGT ACCCCGCCGA CATCCAGGAC TACTTCAAGC AGACCTTCCC CGAGGGCTAC
  421 CACTGGGAGA GGAGCATGAC CTACGAGGAC CAGGGCATCT GCACCGCCAC CAGCAACATC
  481 AGCATGAGGG GCGACTGCTT CTTCTACGAC ATCAGGTTCG ACGGCACCAA CTTCCCCCCC
  541 AACGGCCCCG TGATGCAGAA GAAGACCCTG AAGTGGGAGC CCAGCACCGA GAAGATGTAC
  601 GTGGAGGACG GCGTGCTGAA GGGCGACGTG AACATGAGGC TGCTGCTGGA GGGCGGCGGC
  661 CACTACAGGT GCGACTTCAA GACCACCTAC AAGGCCAAGA AGGACGTGAG GCTGCCCGAC
  721 GCCCACAAGA TCGACCACAG GATCGAGATC CTGAAGCACG ACAAGGACTA CAACAAGGTG
  781 AAGCTGTACG AGAACGCCGT GGCCAGGTAC TCCATGCTGC CCAGCCAGGC CAAGTGAGAA
  841 TTCGAAGCTT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC
  901 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT
  961 TGCTGAAAGG AGGAACTATA TCCGGGATCT GGCGTAATAG CGAAGAGGGC CCGCACCGAT
 1021 CGCTCTTTCC CAACAGTTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGGC
 1081 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTTG ACGGCTAACA CTGGCCAGCG
1141 TCTTAGCGCC CGGCTCGGTT CGCTATTCCG TCCGATCCTT TTCTTCGCCC ACGCGAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl