Please review the QC test results indicated by icon below as well as clone information before placing your order.

hmAG1/pRSETB

Plasmid clone of fluorescent protein monomeric Azami-Green (mAG).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A. (2003) A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278: 34167-34171. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A. (2003) A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278: 34167-34171. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15230_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB15230_A7H9a.seq

>D04498D6_1_A7H9_T7_20bp_B01_02_ABI08.ab1     1 GAAAATTCCC TTTTAAAAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGCGG    61 GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA CTGGTGGACA GCAAATGGGT   121 CGGGATCTGT ACGACGATGA CGATAAGGAT CCCACCATGG TGAGCGTGAT CAAGCCCGAG   181 ATGAAGATCA AGCTGTGCAT GAGGGGCACC GTGAACGGCC ACAACTTCGT GATCGAGGGC   241 GAGGGCAAGG GCAACCCCTA CGAGGGCACC CAGATCCTGG ACCTGAACGT GACCGAGGGC   301 GCCCCCCTGC CCTTCGCCTA CGACATCCTG ACCACCGTGT TCCAGTACGG CAACAGGGCC   361 TTCACCAAGT ACCCCGCCGA CATCCAGGAC TACTTCAAGC AGACCTTCCC CGAGGGCTAC   421 CACTGGGAGA GGAGCATGAC CTACGAGGAC CAGGGCATCT GCACCGCCAC CAGCAACATC   481 AGCATGAGGG GCGACTGCTT CTTCTACGAC ATCAGGTTCG ACGGCACCAA CTTCCCCCCC   541 AACGGCCCCG TGATGCAGAA GAAGACCCTG AAGTGGGAGC CCAGCACCGA GAAGATGTAC   601 GTGGAGGACG GCGTGCTGAA GGGCGACGTG AACATGAGGC TGCTGCTGGA GGGCGGCGGC   661 CACTACAGGT GCGACTTCAA GACCACCTAC AAGGCCAAGA AGGACGTGAG GCTGCCCGAC   721 GCCCACAAGA TCGACCACAG GATCGAGATC CTGAAGCACG ACAAGGACTA CAACAAGGTG   781 AAGCTGTACG AGAACGCCGT GGCCAGGTAC TCCATGCTGC CCAGCCAGGC CAAGTGAGAA   841 TTCGAAGCTT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC   901 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT   961 TGCTGAAAGG AGGAACTATA TCCGGGATCT GGCGTAATAG CGAAGAGGGC CCGCACCGAT  1021 CGCTCTTTCC CAACAGTTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGGC  1081 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTTG ACGGCTAACA CTGGCCAGCG 1141 TCTTAGCGCC CGGCTCGGTT CGCTATTCCG TCCGATCCTT TTCTTCGCCC ACGCGAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl