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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Cy11.5 /pRSET B (#RDB15706)

Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form.

Clone info. Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form. Superior efficiency of FRET.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name Aequorea victoria green fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (partial) RDB15706pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Biochemistry 45: 6267-6271 (2006).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Biochemistry 45: 6267-6271 (2006).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15706 Cy11.5 /pRSET B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Cy11.5 /pRSET B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15706).

Reference section:

Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15706_A7Jqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15706_A7Jqa.seq check
>15706_B0L4F_2_lacIpro-B_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNCN NCGNGTCNGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA
  181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT
  241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCC
  301 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  361 GGCGACGTAA ACGGCCACAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  421 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  481 CTCGTGACCA CCCTGACCTG GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
  541 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG TACCATCTTC
  601 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  661 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
  721 AAGCTGGAGT ACAACTACAT CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGANN
  781 GGCATCAAGG CCCACTTCAA GATCCGCCAC AACATCGAGG ANGGCGGCGT GCAGCTCGCC
  841 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGNCCCG NGCTGCTGNC CGACNACCAC
  901 TACCTGANCA CCCAGNCCGC CCTGANCAAA GNNCCCNACG ANAAGCGNGA TCACATNGNC
  961 CTGCTGGANT NCGNGNNCNN NNNCNNNNNN NANNANCTGN NCACCGGGGN NNNGNCNNTN
1021 CNNNNNNANC NNNNNNNNNN
//
Primer: ECFP-N_F1 (Pr0924)
Region: insert mid
Sequence file: RDB15706_A7Jqb.seq check
>15706_B0L4F_2_ECFP-N_F1_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NGNCNNCTAC GGNAGCTGNN CCTGNAGTTC ATCTGCACCA
   61 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG GGCGTGCAGT
  121 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  181 AAGGCTACGT CCAGGAGCGT ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
  241 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  301 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC AGCCACAACG
  361 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CCACTTCAAG ATCCGCCACA
  421 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
  481 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG
  541 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCCTCGAGG
  601 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
  661 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGANCCTGA
  721 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
  781 GCTACGGCCT GCAGTGCTTC GCCCGCTACC NCGACCACNT GAAGCAGCAC GACTTCTTCA
  841 AGTCCGCCAT GCCCGAANGC TACGTCCAGG ANCGCACCAT CTTCTTCANN GACGACGGCA
  901 ACTACNAGAN CCGCGCCNAN GTGAANTTCG AGGGNNANAC CCTGGNGAAN NNNNTCGAGC
  961 TNNANNGNNN TCGANNNNNN NNANNNNNNN NNCNTCCTGG GGNNNNNNCN NGNANTANNN
1021 CNNNNNANNN NNNNNGTCTA NNNNNNNNNN
//
Primer: T7ter_R1 (Pr0798)
Region: insert 3'
Sequence file: RDB15706_A7Jqc.seq check
>15706_B0L4F_2_T7ter_R1_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCNAC TCAGCTTCCT
   61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGTACAG CTCGTCCATG
  121 CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG
  181 TTGGGGTCTT TGCTCAGGGC GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG
  241 GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCCGCCGTCC
  301 TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG
  361 ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC
  421 TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC
  481 ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG
  541 TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG
  601 GCGAAGCACT GCAGGCCGTA GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC
  661 TTGCCGGTGG TGCAGATCAG CTTCAGGGTC AGCTTGCCGT ANGTGGCATC GCCCTCGCCC
  721 TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC
  781 ACCACCCCGG TGAACAGCTC CTCCTCGAGG GCGGCGGTCA CGAACTCCNG CANGANCATG
  841 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGANT GGGTGCTCAN GTAGTGGTTG
  901 TCGGGCAGCA GCACGGGGCN GTCNCNNATG GGGGNGNTCT GNTGGTAGTG GNCNGNGANC
  961 TGNNCGCCNC CNNCNNCGAT GTTGTGGCGG ATCNNGNAGN GGGNNNNGNN GNNNNNNNNN
1021 N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538. [link to RRC of NBRP]
reference Esposito, A., pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging. Biochemistry 47 (49): 13115-13126 (2008). PMID 19007185.