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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Cy11.5 /pRSET B

Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form.

Catalog number RDB15706
Resource name Cy11.5 /pRSET B
Clone info. Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form. Superior efficiency of FRET.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria green fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Aequorea victoria green fluorescent protein

            Reference sequence
              
             
            Sequence (partial) RDB15706pka01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15706 Cy11.5 /pRSET B DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15706_A7Jqp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
            Sequence file: RDB15706_A7Jqa.seq check
            >15706_B0L4F_2_lacIpro-B_F_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNCNCN NCGNGTCNGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG
               61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA
              121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA
              181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT
              241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCC
              301 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
              361 GGCGACGTAA ACGGCCACAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
              421 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
              481 CTCGTGACCA CCCTGACCTG GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
              541 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG TACCATCTTC
              601 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
              661 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
              721 AAGCTGGAGT ACAACTACAT CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGANN
              781 GGCATCAAGG CCCACTTCAA GATCCGCCAC AACATCGAGG ANGGCGGCGT GCAGCTCGCC
              841 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGNCCCG NGCTGCTGNC CGACNACCAC
              901 TACCTGANCA CCCAGNCCGC CCTGANCAAA GNNCCCNACG ANAAGCGNGA TCACATNGNC
              961 CTGCTGGANT NCGNGNNCNN NNNCNNNNNN NANNANCTGN NCACCGGGGN NNNGNCNNTN
            1021 CNNNNNNANC NNNNNNNNNN
            //
            Primer: ECFP-N_F1
            Region: insert mid
            Sequence file: RDB15706_A7Jqb.seq check
            >15706_B0L4F_2_ECFP-N_F1_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCNN NGNCNNCTAC GGNAGCTGNN CCTGNAGTTC ATCTGCACCA
               61 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG GGCGTGCAGT
              121 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
              181 AAGGCTACGT CCAGGAGCGT ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
              241 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
              301 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC AGCCACAACG
              361 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CCACTTCAAG ATCCGCCACA
              421 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
              481 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG
              541 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCCTCGAGG
              601 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
              661 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGANCCTGA
              721 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
              781 GCTACGGCCT GCAGTGCTTC GCCCGCTACC NCGACCACNT GAAGCAGCAC GACTTCTTCA
              841 AGTCCGCCAT GCCCGAANGC TACGTCCAGG ANCGCACCAT CTTCTTCANN GACGACGGCA
              901 ACTACNAGAN CCGCGCCNAN GTGAANTTCG AGGGNNANAC CCTGGNGAAN NNNNTCGAGC
              961 TNNANNGNNN TCGANNNNNN NNANNNNNNN NNCNTCCTGG GGNNNNNNCN NGNANTANNN
            1021 CNNNNNANNN NNNNNGTCTA NNNNNNNNNN
            //
            Primer: T7ter_R1
            Region: insert 3'
            Sequence file: RDB15706_A7Jqc.seq check
            >15706_B0L4F_2_T7ter_R1_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCNAC TCAGCTTCCT
               61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGTACAG CTCGTCCATG
              121 CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG
              181 TTGGGGTCTT TGCTCAGGGC GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG
              241 GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCCGCCGTCC
              301 TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG
              361 ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC
              421 TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC
              481 ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG
              541 TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG
              601 GCGAAGCACT GCAGGCCGTA GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC
              661 TTGCCGGTGG TGCAGATCAG CTTCAGGGTC AGCTTGCCGT ANGTGGCATC GCCCTCGCCC
              721 TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC
              781 ACCACCCCGG TGAACAGCTC CTCCTCGAGG GCGGCGGTCA CGAACTCCNG CANGANCATG
              841 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGANT GGGTGCTCAN GTAGTGGTTG
              901 TCGGGCAGCA GCACGGGGCN GTCNCNNATG GGGGNGNTCT GNTGGTAGTG GNCNGNGANC
              961 TGNNCGCCNC CNNCNNCGAT GTTGTGGCGG ATCNNGNAGN GGGNNNNGNN GNNNNNNNNN
            1021 N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538.
            reference Esposito, A., pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging. Biochemistry 47 (49): 13115-13126 (2008). PMID 19007185.

            2022.07.27

            GNP_filter3_RDBDEP_html_220727.pl