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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Cy11.5 /pRSET B

Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form.

Catalog number RDB15706
Resource name Cy11.5 /pRSET B
Clone info. Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form. Superior efficiency of FRET.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria green fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15706 Cy11.5 /pRSET B DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB15706pka01.seq

Original reference

original Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15706_A7Jqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15706_A7Jqa.seq check
>15706_B0L4F_2_lacIpro-B_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNCN NCGNGTCNGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA
  181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT
  241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCC
  301 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  361 GGCGACGTAA ACGGCCACAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  421 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  481 CTCGTGACCA CCCTGACCTG GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
  541 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG TACCATCTTC
  601 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  661 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
  721 AAGCTGGAGT ACAACTACAT CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGANN
  781 GGCATCAAGG CCCACTTCAA GATCCGCCAC AACATCGAGG ANGGCGGCGT GCAGCTCGCC
  841 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGNCCCG NGCTGCTGNC CGACNACCAC
  901 TACCTGANCA CCCAGNCCGC CCTGANCAAA GNNCCCNACG ANAAGCGNGA TCACATNGNC
  961 CTGCTGGANT NCGNGNNCNN NNNCNNNNNN NANNANCTGN NCACCGGGGN NNNGNCNNTN
1021 CNNNNNNANC NNNNNNNNNN
//
Primer: ECFP-N_F1
Region: insert mid
Sequence file: RDB15706_A7Jqb.seq check
>15706_B0L4F_2_ECFP-N_F1_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NGNCNNCTAC GGNAGCTGNN CCTGNAGTTC ATCTGCACCA
   61 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG GGCGTGCAGT
  121 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  181 AAGGCTACGT CCAGGAGCGT ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
  241 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  301 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC AGCCACAACG
  361 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CCACTTCAAG ATCCGCCACA
  421 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
  481 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG
  541 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCCTCGAGG
  601 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
  661 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGANCCTGA
  721 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
  781 GCTACGGCCT GCAGTGCTTC GCCCGCTACC NCGACCACNT GAAGCAGCAC GACTTCTTCA
  841 AGTCCGCCAT GCCCGAANGC TACGTCCAGG ANCGCACCAT CTTCTTCANN GACGACGGCA
  901 ACTACNAGAN CCGCGCCNAN GTGAANTTCG AGGGNNANAC CCTGGNGAAN NNNNTCGAGC
  961 TNNANNGNNN TCGANNNNNN NNANNNNNNN NNCNTCCTGG GGNNNNNNCN NGNANTANNN
1021 CNNNNNANNN NNNNNGTCTA NNNNNNNNNN
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB15706_A7Jqc.seq check
>15706_B0L4F_2_T7ter_R1_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCNAC TCAGCTTCCT
   61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGTACAG CTCGTCCATG
  121 CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG
  181 TTGGGGTCTT TGCTCAGGGC GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG
  241 GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCCGCCGTCC
  301 TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG
  361 ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC
  421 TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC
  481 ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG
  541 TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG
  601 GCGAAGCACT GCAGGCCGTA GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC
  661 TTGCCGGTGG TGCAGATCAG CTTCAGGGTC AGCTTGCCGT ANGTGGCATC GCCCTCGCCC
  721 TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC
  781 ACCACCCCGG TGAACAGCTC CTCCTCGAGG GCGGCGGTCA CGAACTCCNG CANGANCATG
  841 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGANT GGGTGCTCAN GTAGTGGTTG
  901 TCGGGCAGCA GCACGGGGCN GTCNCNNATG GGGGNGNTCT GNTGGTAGTG GNCNGNGANC
  961 TGNNCGCCNC CNNCNNCGAT GTTGTGGCGG ATCNNGNAGN GGGNNNNGNN GNNNNNNNNN
1021 N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538.
reference Esposito, A., pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging. Biochemistry 47 (49): 13115-13126 (2008). PMID 19007185.

2021.02.18

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