Resource data sheet
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Cy11.5 /pRSET B

Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form.

Catalog number RDB15706
Resource name Cy11.5 /pRSET B
Clone info. Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form. Superior efficiency of FRET.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria green fluorescent protein cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15706 Cy11.5 /pRSET B Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538.
reference Esposito, A., pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging. Biochemistry 47 (49): 13115-13126 (2008). PMID 19007185.

2019.07.17

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