RDB15706 - Cy11.5 /pRSET B

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

Cy11.5 /pRSET B (#RDB15706)

Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form.

Clone info.	Chimeric fluorescent protein. CFP-YFP fusion protein of concatenation form. Superior efficiency of FRET.
Vector backbone	pRSET B (Plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	Aequorea victoria green fluorescent protein cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Sequence (partial)	RDB15706pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15706	Cy11.5 /pRSET B	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Cy11.5 /pRSET B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15706).

Reference section:

Shimozono, S., Hosoi, H., Mizuno, H., Fukano, T., Tahara, T., Miyawaki, A., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15706_A7Jqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15706_A7Jqa.seq
>15706_B0L4F_2_lacIpro-B_F_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNCNCN NCGNGTCNGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG 61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA 121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA 181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT 241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCC 301 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 361 GGCGACGTAA ACGGCCACAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC 421 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC 481 CTCGTGACCA CCCTGACCTG GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG 541 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG TACCATCTTC 601 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG 661 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC 721 AAGCTGGAGT ACAACTACAT CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGANN 781 GGCATCAAGG CCCACTTCAA GATCCGCCAC AACATCGAGG ANGGCGGCGT GCAGCTCGCC 841 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGNCCCG NGCTGCTGNC CGACNACCAC 901 TACCTGANCA CCCAGNCCGC CCTGANCAAA GNNCCCNACG ANAAGCGNGA TCACATNGNC 961 CTGCTGGANT NCGNGNNCNN NNNCNNNNNN NANNANCTGN NCACCGGGGN NNNGNCNNTN 1021 CNNNNNNANC NNNNNNNNNN //
Primer: ECFP-N_F1 (Pr0924) Region: insert mid Sequence file: RDB15706_A7Jqb.seq
>15706_B0L4F_2_ECFP-N_F1_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNN NGNCNNCTAC GGNAGCTGNN CCTGNAGTTC ATCTGCACCA 61 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG GGCGTGCAGT 121 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG 181 AAGGCTACGT CCAGGAGCGT ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG 241 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 301 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC AGCCACAACG 361 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CCACTTCAAG ATCCGCCACA 421 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG 481 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG 541 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCCTCGAGG 601 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 661 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGANCCTGA 721 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG 781 GCTACGGCCT GCAGTGCTTC GCCCGCTACC NCGACCACNT GAAGCAGCAC GACTTCTTCA 841 AGTCCGCCAT GCCCGAANGC TACGTCCAGG ANCGCACCAT CTTCTTCANN GACGACGGCA 901 ACTACNAGAN CCGCGCCNAN GTGAANTTCG AGGGNNANAC CCTGGNGAAN NNNNTCGAGC 961 TNNANNGNNN TCGANNNNNN NNANNNNNNN NNCNTCCTGG GGNNNNNNCN NGNANTANNN 1021 CNNNNNANNN NNNNNGTCTA NNNNNNNNNN //
Primer: T7ter_R1 (Pr0798) Region: insert 3' Sequence file: RDB15706_A7Jqc.seq
>15706_B0L4F_2_T7ter_R1_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCNAC TCAGCTTCCT 61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGTACAG CTCGTCCATG 121 CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG 181 TTGGGGTCTT TGCTCAGGGC GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG 241 GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCCGCCGTCC 301 TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG 361 ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC 421 TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC 481 ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG 541 TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG 601 GCGAAGCACT GCAGGCCGTA GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC 661 TTGCCGGTGG TGCAGATCAG CTTCAGGGTC AGCTTGCCGT ANGTGGCATC GCCCTCGCCC 721 TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC 781 ACCACCCCGG TGAACAGCTC CTCCTCGAGG GCGGCGGTCA CGAACTCCNG CANGANCATG 841 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGANT GGGTGCTCAN GTAGTGGTTG 901 TCGGGCAGCA GCACGGGGCN GTCNCNNATG GGGGNGNTCT GNTGGTAGTG GNCNGNGANC 961 TGNNCGCCNC CNNCNNCGAT GTTGTGGCGG ATCNNGNAGN GGGNNNNGNN GNNNNNNNNN 1021 N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shimozono, S., Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Biochemistry 45 (20): 6267-6271 (2006). PMID 16700538. [link to RRC of NBRP]
reference	Esposito, A., pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging. Biochemistry 47 (49): 13115-13126 (2008). PMID 19007185.

Resource search

Link

Other resources in our bank

External Database
Aequorea victoria green fluorescent protein

Reference sequence