RDB15449 - tFucci(SA)2/pCSII-CMV

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tFucci(SA)2/pCSII-CMV (#RDB15449)

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.

Clone info.	Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.
Vector backbone	CSII-CMV-MCS (RDB04377) (plasmid)
Size of vector backbone	8.6 kb
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions	37oC, LB + Amp
Gene/insert name	Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	LC192886 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Sakaue-Sawano, A.et al., Mol. Cell 68 (3): 626-640.e5 (2017).
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A.et al., Mol. Cell 68 (3): 626-640.e5 (2017). Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A.et al., Mol. Cell 68 (3): 626-640.e5 (2017). 付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15449	tFucci(SA)2/pCSII-CMV	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The tFucci(SA)2/pCSII-CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15449).

Reference section:

Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15449_A7Jvp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R (Pr0517) Region: insert 5'(mCherry 5'),CMV pro.2 Sequence file: RDB15449_A7Jvg.seq
>15449_15449_A9GnF_mCherry-C_R_A12_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNT NNNNGTTGNN GTTGNNGGCG CCGGGCAGCT GCNCGGGCTT 61 CTTGGCCTTG TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG 121 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC 181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG 241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC 301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG 361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA 421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT 481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG 541 GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT 601 GTTATCCTCC TCGCCCTTGC TCACCATGGT GGATCCTATG CTAGCGGATC TGACGGTTCA 661 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA 721 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CNAAACAAAC 781 TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC 841 GCCCATTGAT GTACTGCCAA AACCGCATCA CCATNGTAAT AGCGATGANT AATACGTAGA 901 TGTACTGCCA AGTANGAAAG TNCCNNNANN NNCATGTACT NNNCATAATG NCAGGCNGGN 961 CNTTTACCNT CATTGACGTN ANNAGGGGGC GNANTTNNNN NATGANACAC TNNNNGNANT 1021 GNCNANNNNN NNTTTANNNN NNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(Gem1 (1-110),mVenus 3') Sequence file: RDB15449_A7Jvh.seq
>D05018A9_2_A7Jv_PRE_rev_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN NGNNCNNNAT AGTTAAGAAT ACCNGTCAAT CTTTCNCAAA 61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGATTA 121 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT 181 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC 241 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT 301 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG 361 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC 421 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC 481 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC 541 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGTAGC TCAGGTAGTG 601 GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC 661 GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTGGCCT TGATGCCGTT 721 CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CCAGCTTGTG 781 CCCCAGGATG TTGCCGTCCT CCTTGAANTC NANGCCCTTC AGCTCGATGC GNNTCACCAN 841 GGNGTCGCCC TCNAACTTCN NNNCNGCGCG GGTCTTGNAN TTGCCGNCNN CCNNNNNNAN 901 NNNGGNGCGC NNCNNNNNNG NNNNCNNNNN GNNNNNNNNG NNNNTNNNNN NNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi,5' LTR,CMV pro.1 Sequence file: RDB15449_A7Jvi.seq
>15449_15449_B3I4F_HIV-B_R_E03_15_ABI24.ab1 1 NNNNNNNNNN CNNNNNAAAN TTTTGGCGTA NTCNCNAGTC GCCGCCCCTC GCCTCTTGCC 61 GTGCGCGCTT CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT 121 TTCAAGTCCC TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA 181 GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG 241 GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC 301 AGATCTGGTC TAACCAGAGA GACCCAGTAC AGGCAAAACG CGCTGCTTAT ATAGACCTCC 361 CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG ACATTTTGGA 421 AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG GTGGAGACTT 481 GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA AAACCGCATC 541 ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA GTCCCATAAG 601 GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC AATAGGGGGC 661 GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG TAAATACTCC 721 ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA CGTCATTATT 781 GACGTCNANG GGCGGGGGTC GTNNGGNGGN CANCCAGGNG GGCCATTNAC NNNAANNNAT 841 GNAANGCGGA NNNNCATANA NNGGNCNANN AACNNANNAN CCCNNNNANN NNNNNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5' LTR Sequence file: RDB15449_A7Jvj.seq
>D05018A9_2_A7Jv_HIVgp_R1_A11_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NCTTTTTTCT CTCTGCNCCA CTCTTCTCTT TGCCTTGGTG 61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA 121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG 181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA 241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT 301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG 361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA 421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG 481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC 541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG 601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT 661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAANGGTC TGANGGANCT 721 CTAGTTACCA GANTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC NAGGCAAGCT 781 TTANNGANGC TTAANCANNG GGTTNCCNAN TTANCNNGAN ANCTCCNANG NTNANNNCNN 841 GTCNNACNNG ANAGANCNNG NACNNNNNAA ANGNNCTNNN NATATANNNN NNCNNNCNNN 901 NNNNNCNNNN NNNCNNNTNN NNNNNNNN //
Primer: CSII-CMV5' (Pr0021) Region: insert 5'(mCherry 3') Sequence file: RDB15449_A7Jvk.seq
>D05018A9_2_A7Jv_CSII-CMV5'_C11_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNGNNC NNGGGCGANG NNGNNAACAT GGCCATCATC 61 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG 121 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG 181 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC 241 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC 301 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC 361 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC 421 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG 481 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG 541 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG 601 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC 661 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG 721 CTGTACAAGG GATATCCATC ACACTGGCGN CCGCTCGAGC CCAGCCCCGC CAGGCCCGCA 781 CTCCGCGCCC CGGCCTCCGC TACCAGTGGC AGCCGCAAGC GCGCCCGCCC GCCCGCCGCC 841 CCCNGGACGC NANCAGGNCA GGNNACCGGN CCGCNNNANA NNGNNGCTNN CGGNGGANNA 901 NNTTTCCANC CCCNGTACCC CCCNANGCCC NNGANNTCCN NNNCNNNNNC NNNNNNNNGG 961 NNNNNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid(mCherry 3',Cdt1 (30-120),P2A,mVenus 5') Sequence file: RDB15449_A7Jvl.seq
>D05018A9_2_A7Jv_mCherry-C_F_H03_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCCNGGNN CCTANNCGTC AACNTCAAGT TGGACATCAC 61 CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC 121 CACCGGCGGC ATGGACGAGC TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGCC 181 CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA GCCGCAAGCG 241 CGCCCGCCCG CCCGCCGCCC CCGGACGCGA CCAGGCCAGG CCACCGGCCC GCAGGAGACT 301 GCGGCTGTCG GTGGACGAGG TTTCCAGCCC CAGTACCCCC GAGGCCCCAG ACATCCCAGC 361 CTGCCCTTCT CCGGGCCAGA AGATAAAGAA ATCCACCCCG GCAGCAGGTC AGCCGCCCCA 421 CCTGACATCC GCGCAGGACC AGGACACCAT CACCGGTCGC GCCAAGCGCG GCAGCGGCGC 481 CACCAACTTC AGCCTGCTGA AGCAGGCCGG CGACGTGGAG GAGAACCCCG GCCCCCGCGA 541 ATTCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT 601 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC 661 CTACGGCAAG CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 721 CACCCTCGTG ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT 781 GAAGCAGCAC GACTTCTTCA AGTCCGCCAN GCCCGAAGGC TACGTCCAGG NNCGCACCAT 841 CTTCTTCAAN GANGACGGCN NCTACNAGAN CCGCGCCNAN GTGAAGTNCN NNGGCGANNC 901 CCNNNNNAAC CNNATCNNNC TGAANGGNNT CNNNTNNNAN NNANGNANGG CNNCATCCTN 961 NGGCNNNNNN NNGNNNNNNN NNN //
Primer: EGFP-N_F (Pr0649) Region: insert mid(mVenus mid) Sequence file: RDB15449_A7Jvm.seq
>15449_15449_B3I4F_EGFP-N_F_F03_18_ABI24.ab1 1 NNNNNNNNNN NNGNNNNNNC NNNGGCGNNG CNNCCTACNG CANNCTGNNC CTNAANCTGN 61 NCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG 121 GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG 181 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA 241 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG 301 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA 361 GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA 421 TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC 481 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCAAGC 541 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG 601 CCGGGATCAC TCTCGGCATG GACGANCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC 661 TCGAGATGAA TCCCAGTATG AAGCAGAAAC AAGAAGAAAT CAAAGANAAT ATANAGAATA 721 GTTCTGTCNN NAGAAGAACT CTGAAGANGA NTCAGCCTTC TGCATCTGNN TCTCTNGTTG 781 GAAGANAAAN TGANCTGTCC GCNNNNTTGT CCAAAANGAA ACATCGGNAN TGANNACTTA 841 ACATCTANNN NTTCCAGCCN NNGGGNTNAN NNNNCNNNNN NCNNNNNNAA NNNAAAANNN 901 NNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: 3' LTR Sequence file: RDB15449_A7Jvn.seq
>15449_15449_B3I4F_bGH_rev3_G03_21_ABI24.ab1 1 NNNNNNNNNN NNNNGNNGNN GGGNANNAAC ANNTGGCTGG CAACTAGANN GCACAGTCGN 61 GGCTGNTCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC 121 TGAGGGNTCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC 181 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG 241 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT CGGGCCATGT 301 GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC GGGTCATCCA 361 TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG GCTTCTTCTA 421 CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT GGATATCTGA 481 TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG TGGTAGATCC 541 ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGANTTAG CCCTTCCAGT CCCCCCTTTT 601 CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT AAAGGTACCT 661 GANGTGTGAN TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG GCACAATCAG 721 CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCNTG TTTTTCTANN TCTCGACGGT 781 ATCNATGCNG GGNNGNNGCC CAAANGGANA TNCGANNCNN CTGANGNCGA ANNNCGAANA 841 NNCNNNNNAN GNNNCANNNN CNNNANCNNN CCNNNNNNAN NNNNNNTCNN NNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro,BleoR,SV40 pA Sequence file: RDB15449_A7Jvo.seq
>D05018A9_2_A7Jv_SV40pro_F_B11_05_ABI24 1 NNNNNNNNNN NNNNNNNNNN ANNNNTGNNG NNGCTTTTTT GGANGCCTAG GCTTTTGCNA 61 AAAGCTCCCG GGANCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA 121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC 181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC 241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC 301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC 361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG 421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG 481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG 541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCNA CCTCTAGCTA GANCTTGGCG 841 TAATCATGGT CATAGCTGTT TCCTGNGTGA AATTGTTATC CGCNCACAAT TCCNCACAAC 901 ATACNANCCN GAAGCNTAAA GNNNNAAGNN NGGGGNNNNN TAANGNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

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External Database
Human CDT1

Human Geminin

Discosoma sp. RFP

Aequorea victoria GFP

Reference sequence