Sequencing and PCR primers for primer information.
Lentivirus vector construct of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.
| Catalog number | RDB15449 |
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| Resource name | tFucci(SA)2/pCSII-CMV |
| Clone info. | Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Vector backbone | CSII-CMV-MCS (plasmid) |
| Size of vector backbone | 8.6 kb |
| Selectable markers | Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. |
| Gene/insert name | Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA |
| Reference of nucleotide sequence | LC192886 (DDBJ accession number) |
| Originator | Miyawaki, Atsushi | |
| Other clones in our bank | human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) | |
| Terms and conditions for distribution | 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. |
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| Ordering | Please visit Ordering instruction. Please contact us. UCSD's MTA is also required. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Remember that you will be working with samples containing infectious virus. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15449 | tFucci(SA)2/pCSII-CMV | DNA solution |
Electronic file
Original reference
| original | Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15449_A7Jvp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: mCherry-C_R | RDB15449_A7Jva.seq |
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>15449_15449_A9GnF_mCherry-C_R_A12_01_ABI08.ab1 1 CGTTGGAAGT TAGGTCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT 61 CTTGGCCTTG TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG 121 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC 181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG 241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC 301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG 361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA 421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT 481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG 541 GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT 601 GTTATCCTCC TCGCCCTTGC TCACCATGGT GGATCCTATG CTAGCGGATC TGACGGTTCA 661 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA 721 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CAAAACAAAC 781 TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC 841 GCCCATTGAT GTACTGCCAA AACCGCATCA CCATGGTAAT AGCGATGACT AATACGTAGA 901 TGTACTGCCA AGTAGGAAAG TCCCATAAAG GTCATGTACT GGGCATAATG CCAGGCGGGC 961 CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT 1021 GCCAAGTGGC AGTTTACCGT AATACTTCCA ACCCATTTGA CGTCAATGGA AAGTCCTATT 1081 TGGCCGTTTA CTATGGGAAC ATACGTCATT AATTGACGTT CAATGGGCGG GGGTCGGTGG 1141 GCCGATCAAG CTCAGGCCGG GCCAATTTAA CCCGGTTA // |
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| Primer: BGH_rev | RDB15449_A7Jvb.seq |
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>D05018A9_2_A7Jv_BGH_rev_F11_17_ABI24.ab1 1 GTATACAGGG GCGGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT 61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT 121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA 181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC 241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA 301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA 361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG 421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT 481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC 541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA 601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG 661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG 721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA 781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC 841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG 901 TCAGCTTCCC CGACAACACC ACGGAAATTG TCAGTGCCCA AACAGCCGAG CCCCTGTCCA 961 GCAGCGGGCA AGCAGGCGGC GATGAGTTCC GCCGTGCCAA TAGGAGGGGG GAAAGCGGAA 1021 AGTCCGGAAA GAGCTGAACA GTTGATTGCA TGCCCCACCA GTGAGTTGCG TTCAGTAAAC 1081 ACAGTGCACA AACCCAACGT CCAGCGT // |
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| Primer: mCherry-C_F | RDB15449_A7Jvc.seq |
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>D05018A9_2_A7Jv_mCherry-C_F_H03_08_ABI08.ab1 1 AACCGGAAGC CCGGTGCAGG CTGCCCGGCG CCTACACGTC AACATCAAGT TGGACATCAC 61 CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC 121 CACCGGCGGC ATGGACGAGC TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGCC 181 CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA GCCGCAAGCG 241 CGCCCGCCCG CCCGCCGCCC CCGGACGCGA CCAGGCCAGG CCACCGGCCC GCAGGAGACT 301 GCGGCTGTCG GTGGACGAGG TTTCCAGCCC CAGTACCCCC GAGGCCCCAG ACATCCCAGC 361 CTGCCCTTCT CCGGGCCAGA AGATAAAGAA ATCCACCCCG GCAGCAGGTC AGCCGCCCCA 421 CCTGACATCC GCGCAGGACC AGGACACCAT CACCGGTCGC GCCAAGCGCG GCAGCGGCGC 481 CACCAACTTC AGCCTGCTGA AGCAGGCCGG CGACGTGGAG GAGAACCCCG GCCCCCGCGA 541 ATTCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT 601 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC 661 CTACGGCAAG CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 721 CACCCTCGTG ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT 781 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT 841 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC 901 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAA GGAGGGACGG CAACATCCTG 961 GGGCACAAGC TGGGAGTACA ACTACACAGC CACAACGTCT ATATCACCGC CCGACAGGCA 1021 GAAGGAACGG CATCAAGGCC AACTTCCAGA TCCGCCACAA CATCGAGGAA CGCGGCCGTG 1081 CCAGCTCGCC CCGAACTCCA ACT // |
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| Primer: HIVgp_R1 | RDB15449_A7Jvd.seq |
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>D05018A9_2_A7Jv_HIVgp_R1_A11_02_ABI24.ab1 1 CAGGCATCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG 61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA 121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG 181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA 241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT 301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG 361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA 421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG 481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC 541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG 601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT 661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT 721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT 781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG 841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA 901 CACGCCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGGAAAGTC 961 CCGTGATTTT TGTGCCAAAA CAAACTCCCA TGACGTCATG GGGTGGAGAC TCAATTCCCC 1021 GTGGAGTCAA CCCGCTATCA CGCCATGGAA TGTACTGGCA AACGCATCAC ATGCTATAGC 1081 GAATGACCTA ATACGGCTA // |
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| Primer: BGH_rev | RDB15449_A7Jve.seq |
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>D05018A9_2_A7Jv_BGH_rev_F11_17_ABI24.ab1 1 GTATACAGGG GCGGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT 61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT 121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA 181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC 241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA 301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA 361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG 421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT 481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC 541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA 601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG 661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG 721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA 781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC 841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG 901 TCAGCTTCCC CGACAACACC ACGGAAATTG TCAGTGCCCA AACAGCCGAG CCCCTGTCCA 961 GCAGCGGGCA AGCAGGCGGC GATGAGTTCC GCCGTGCCAA TAGGAGGGGG GAAAGCGGAA 1021 AGTCCGGAAA GAGCTGAACA GTTGATTGCA TGCCCCACCA GTGAGTTGCG TTCAGTAAAC 1081 ACAGTGCACA AACCCAACGT CCAGCGT // |
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| Primer: SV40pro_F | RDB15449_A7Jvf.seq |
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>D05018A9_2_A7Jv_SV40pro_F_B11_05_ABI24.ab1 1 CCTGGTCCCT GGACTTTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA 121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC 181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC 241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC 301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC 361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG 421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG 481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG 541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG 841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC 961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCCAGTCGGG AAACCCTGGT CGTGCCAGCC 1021 TGCATTTAAA TGAATTCCGT CACCGCCGCC GGGAGGAGGC GTTGCCGTAT GGCGCTTCAT 1081 CCCGCGTTCC CCTTCGC // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. |
|---|
2019.07.26
GNP_filter3_RDBDEP_html_190432.pl