Sequencing and PCR primers for primer information.
Lentivirus vector construct of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.
| Catalog number | RDB15449 |
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| Resource name | tFucci(SA)2/pCSII-CMV |
| Clone info. | Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Vector backbone | CSII-CMV-MCS (plasmid) |
| Size of vector backbone | 8.6 kb |
| Selectable markers | Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells). |
| Gene/insert name | Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA |
| Reference of nucleotide sequence | LC192886 (DDBJ accession number) |
| Originator | Miyawaki, Atsushi | |
| Other clones in our bank | human CDT1 (NCBI Gene 81620) | |
| Terms and conditions for distribution | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. |
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| Ordering | Please visit Ordering instruction. Please contact us. UCSD's MTA is also required. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
| Remarks | ((Additional form)) Dr. Miyawaki's Approval ((Remarks)) Remember that you will be working with samples containing infectious virus. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15449 | tFucci(SA)2/pCSII-CMV | DNA solution |
Electronic file
Featured content
References
| original | Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15449_A7Jvp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CSII-CMV5' | RDB15449_A7Jva.seq |
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>D05018A9_2_A7Jv_CSII-CMV5'_C11_08_ABI24.ab1 1 AACTGCCAGC ATAGGATCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC 61 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG 121 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG 181 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC 241 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC 301 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC 361 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC 421 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG 481 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG 541 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG 601 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC 661 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG 721 CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGC CCAGCCCCGC CAGGCCCGCA 781 CTCCGCGCCC CGGCCTCCGC TACCAGTGGC AGCCGCAAGC GCGCCCGCCC GCCCGCCGCC 841 CCCCGGACGC GACCAGGCCA GGCCACCGGC CCGCAGGAGA CTGCGGCTGT CGGTGGACGA 901 GGTTTCCAGC CCCAGTACCC CCCGAGGCCC CAGACATCCC AGCCCTGCCC CTTCTCCGGG 961 GCCAGAGATA AGAAATCCCA CCCCCGGCAG CAGTCAGCCC GCCCCACCCT GACATCCGCG 1021 CAAGAACCAA GGACACCATT CAACGGGTTC GCGCTAGCGC GCAGCGACGT CATCGAACCT 1081 TCCAGTCCTG GCTTGAAGTC CAG // |
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| Primer: mCherry-C_F | RDB15449_A7Jvb.seq |
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>D05018A9_2_A7Jv_mCherry-C_F_H03_08_ABI08.ab1 1 AACCGGAAGC CCGGTGCAGG CTGCCCGGCG CCTACACGTC AACATCAAGT TGGACATCAC 61 CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC 121 CACCGGCGGC ATGGACGAGC TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGCC 181 CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA GCCGCAAGCG 241 CGCCCGCCCG CCCGCCGCCC CCGGACGCGA CCAGGCCAGG CCACCGGCCC GCAGGAGACT 301 GCGGCTGTCG GTGGACGAGG TTTCCAGCCC CAGTACCCCC GAGGCCCCAG ACATCCCAGC 361 CTGCCCTTCT CCGGGCCAGA AGATAAAGAA ATCCACCCCG GCAGCAGGTC AGCCGCCCCA 421 CCTGACATCC GCGCAGGACC AGGACACCAT CACCGGTCGC GCCAAGCGCG GCAGCGGCGC 481 CACCAACTTC AGCCTGCTGA AGCAGGCCGG CGACGTGGAG GAGAACCCCG GCCCCCGCGA 541 ATTCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT 601 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC 661 CTACGGCAAG CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 721 CACCCTCGTG ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT 781 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT 841 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC 901 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAA GGAGGGACGG CAACATCCTG 961 GGGCACAAGC TGGGAGTACA ACTACACAGC CACAACGTCT ATATCACCGC CCGACAGGCA 1021 GAAGGAACGG CATCAAGGCC AACTTCCAGA TCCGCCACAA CATCGAGGAA CGCGGCCGTG 1081 CCAGCTCGCC CCGAACTCCA ACT // |
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| Primer: PRE_rev | RDB15449_A7Jvc.seq |
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>D05018A9_2_A7Jv_PRE_rev_E11_14_ABI24.ab1 1 CGCAATCAAA ATAGCGTAAA GGAGCAACAT AGTTAAGAAT ACCAGTCAAT CTTTCACAAA 61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGATTA 121 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT 181 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC 241 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT 301 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG 361 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC 421 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC 481 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC 541 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGTAGC TCAGGTAGTG 601 GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC 661 GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTGGCCT TGATGCCGTT 721 CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CCAGCTTGTG 781 CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC GGTTCACCAG 841 GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA 901 GATGGTGCGC TCCCTGGACG TAGCCCTTCG GGCATGGCGG GACTTTGAAG AAGTCGTGCT 961 GCTTCATGTG GTCGGGGTAG CGGGCGGAGC ACTGCAGACG TAGCCCAGGG TTGATCACGA 1021 GGATGGGTCA GGGCACGGCA GCTGGCGTTG ATGCAGATCA GCTCAAGTCA GCTGCGTAGT 1081 ACCATCGC // |
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| Primer: HIVgp_R1 | RDB15449_A7Jvd.seq |
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>D05018A9_2_A7Jv_HIVgp_R1_A11_02_ABI24.ab1 1 CAGGCATCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG 61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA 121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG 181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA 241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT 301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG 361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA 421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG 481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC 541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG 601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT 661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT 721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT 781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG 841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA 901 CACGCCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGGAAAGTC 961 CCGTGATTTT TGTGCCAAAA CAAACTCCCA TGACGTCATG GGGTGGAGAC TCAATTCCCC 1021 GTGGAGTCAA CCCGCTATCA CGCCATGGAA TGTACTGGCA AACGCATCAC ATGCTATAGC 1081 GAATGACCTA ATACGGCTA // |
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| Primer: BGH_rev | RDB15449_A7Jve.seq |
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>D05018A9_2_A7Jv_BGH_rev_F11_17_ABI24.ab1 1 GTATACAGGG GCGGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT 61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT 121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA 181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC 241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA 301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA 361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG 421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT 481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC 541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA 601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG 661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG 721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA 781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC 841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG 901 TCAGCTTCCC CGACAACACC ACGGAAATTG TCAGTGCCCA AACAGCCGAG CCCCTGTCCA 961 GCAGCGGGCA AGCAGGCGGC GATGAGTTCC GCCGTGCCAA TAGGAGGGGG GAAAGCGGAA 1021 AGTCCGGAAA GAGCTGAACA GTTGATTGCA TGCCCCACCA GTGAGTTGCG TTCAGTAAAC 1081 ACAGTGCACA AACCCAACGT CCAGCGT // |
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| Primer: SV40pro_F | RDB15452_A7Jvf.seq |
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>D05018B3_1_A7Jv_SV40pro_F_B12_06_ABI24.ab1 1 CATTGGTCCC TGGGACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT 121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG 181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT 241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG 301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA 361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG 421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT 481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG 541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGGTG CCTAAATGAG TGAGCTAACT 961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCCTG TCGTGCAGCC 1021 TGCCATTTAA TGAAATCCGG CTACGGCCGC CGGGAGAGGC CGGATTGCCG TATGGGGCGC 1081 TCTTCCGCTT TCCTTCGG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. |
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2018.10.09
GNP_filter3_RDBDEP_html_180823.pl