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tFucci(SA)2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.

Catalog number RDB15449
Resource name tFucci(SA)2/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC192886 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15449 tFucci(SA)2/pCSII-CMV DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15449_A7Jvp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: mCherry-C_R
        Sequence file: RDB15449_A7Jva.seq check
        >15449_15449_A9GnF_mCherry-C_R_A12_01_ABI08.ab1
            1 CGTTGGAAGT TAGGTCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT
           61 CTTGGCCTTG TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
          121 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC
          181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG
          241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC
          301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG
          361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA
          421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT
          481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG
          541 GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT
          601 GTTATCCTCC TCGCCCTTGC TCACCATGGT GGATCCTATG CTAGCGGATC TGACGGTTCA
          661 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA
          721 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CAAAACAAAC
          781 TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC
          841 GCCCATTGAT GTACTGCCAA AACCGCATCA CCATGGTAAT AGCGATGACT AATACGTAGA
          901 TGTACTGCCA AGTAGGAAAG TCCCATAAAG GTCATGTACT GGGCATAATG CCAGGCGGGC
          961 CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT
         1021 GCCAAGTGGC AGTTTACCGT AATACTTCCA ACCCATTTGA CGTCAATGGA AAGTCCTATT
         1081 TGGCCGTTTA CTATGGGAAC ATACGTCATT AATTGACGTT CAATGGGCGG GGGTCGGTGG
        1141 GCCGATCAAG CTCAGGCCGG GCCAATTTAA CCCGGTTA
        //
        Primer: BGH_rev
        Sequence file: RDB15449_A7Jvb.seq check
        >D05018A9_2_A7Jv_BGH_rev_F11_17_ABI24.ab1
            1 GTATACAGGG GCGGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
           61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
          121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
          181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
          241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
          301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
          361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
          421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
          481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
          541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
          601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
          661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
          721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
          781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
          841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
          901 TCAGCTTCCC CGACAACACC ACGGAAATTG TCAGTGCCCA AACAGCCGAG CCCCTGTCCA
          961 GCAGCGGGCA AGCAGGCGGC GATGAGTTCC GCCGTGCCAA TAGGAGGGGG GAAAGCGGAA
         1021 AGTCCGGAAA GAGCTGAACA GTTGATTGCA TGCCCCACCA GTGAGTTGCG TTCAGTAAAC
        1081 ACAGTGCACA AACCCAACGT CCAGCGT
        //
        Primer: mCherry-C_F
        Sequence file: RDB15449_A7Jvc.seq check
        >D05018A9_2_A7Jv_mCherry-C_F_H03_08_ABI08.ab1
            1 AACCGGAAGC CCGGTGCAGG CTGCCCGGCG CCTACACGTC AACATCAAGT TGGACATCAC
           61 CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC
          121 CACCGGCGGC ATGGACGAGC TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGCC
          181 CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA GCCGCAAGCG
          241 CGCCCGCCCG CCCGCCGCCC CCGGACGCGA CCAGGCCAGG CCACCGGCCC GCAGGAGACT
          301 GCGGCTGTCG GTGGACGAGG TTTCCAGCCC CAGTACCCCC GAGGCCCCAG ACATCCCAGC
          361 CTGCCCTTCT CCGGGCCAGA AGATAAAGAA ATCCACCCCG GCAGCAGGTC AGCCGCCCCA
          421 CCTGACATCC GCGCAGGACC AGGACACCAT CACCGGTCGC GCCAAGCGCG GCAGCGGCGC
          481 CACCAACTTC AGCCTGCTGA AGCAGGCCGG CGACGTGGAG GAGAACCCCG GCCCCCGCGA
          541 ATTCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
          601 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
          661 CTACGGCAAG CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
          721 CACCCTCGTG ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT
          781 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
          841 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
          901 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAA GGAGGGACGG CAACATCCTG
          961 GGGCACAAGC TGGGAGTACA ACTACACAGC CACAACGTCT ATATCACCGC CCGACAGGCA
         1021 GAAGGAACGG CATCAAGGCC AACTTCCAGA TCCGCCACAA CATCGAGGAA CGCGGCCGTG
        1081 CCAGCTCGCC CCGAACTCCA ACT
        //
        Primer: HIVgp_R1
        Sequence file: RDB15449_A7Jvd.seq check
        >D05018A9_2_A7Jv_HIVgp_R1_A11_02_ABI24.ab1
            1 CAGGCATCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
           61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
          121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
          181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
          241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
          301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
          361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
          421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
          481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
          541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
          601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
          661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
          721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
          781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
          841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA
          901 CACGCCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGGAAAGTC
          961 CCGTGATTTT TGTGCCAAAA CAAACTCCCA TGACGTCATG GGGTGGAGAC TCAATTCCCC
         1021 GTGGAGTCAA CCCGCTATCA CGCCATGGAA TGTACTGGCA AACGCATCAC ATGCTATAGC
        1081 GAATGACCTA ATACGGCTA
        //
        Primer: BGH_rev
        Sequence file: RDB15449_A7Jve.seq check
        >D05018A9_2_A7Jv_BGH_rev_F11_17_ABI24.ab1
            1 GTATACAGGG GCGGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
           61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
          121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
          181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
          241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
          301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
          361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
          421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
          481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
          541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
          601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
          661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
          721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
          781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
          841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
          901 TCAGCTTCCC CGACAACACC ACGGAAATTG TCAGTGCCCA AACAGCCGAG CCCCTGTCCA
          961 GCAGCGGGCA AGCAGGCGGC GATGAGTTCC GCCGTGCCAA TAGGAGGGGG GAAAGCGGAA
         1021 AGTCCGGAAA GAGCTGAACA GTTGATTGCA TGCCCCACCA GTGAGTTGCG TTCAGTAAAC
        1081 ACAGTGCACA AACCCAACGT CCAGCGT
        //
        Primer: SV40pro_F
        Sequence file: RDB15449_A7Jvf.seq check
        >D05018A9_2_A7Jv_SV40pro_F_B11_05_ABI24.ab1
            1 CCTGGTCCCT GGACTTTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
           61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
          121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
          181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
          241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
          301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
          361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
          421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
          481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
          541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
          601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
          661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
          721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
          781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
          841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
          901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC
          961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCCAGTCGGG AAACCCTGGT CGTGCCAGCC
         1021 TGCATTTAAA TGAATTCCGT CACCGCCGCC GGGAGGAGGC GTTGCCGTAT GGCGCTTCAT
        1081 CCCGCGTTCC CCTTCGC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl