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tFucci(SA)2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.

Catalog number RDB15449
Resource name tFucci(SA)2/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). CMV promoter.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC192886 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions:
        The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering
        Order form [Credit Card Payment]  [Bank Transfer Payment]
        MTA, for use for not-for-profit academic purpose [Word]
        Please visit Ordering instruction.[link] 
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件:
        レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼
        提供依頼書 [Word]
        提供同意書 (MTA, 非営利学術目的用)[Word]
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15449 tFucci(SA)2/pCSII-CMV DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The tFucci(SA)2/pCSII-CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15449).

        Reference section:

        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


        Tips

        Featured content

        Featured content Lentiviral plasmid list (English text)
        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15449_A7Jvp1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: mCherry-C_R (Pr0517)
        Region: insert 5'(mCherry 5'),CMV pro.2
        Sequence file: RDB15449_A7Jvg.seq check
        >15449_15449_A9GnF_mCherry-C_R_A12_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNT NNNNGTTGNN GTTGNNGGCG CCGGGCAGCT GCNCGGGCTT
           61 CTTGGCCTTG TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
          121 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC
          181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG
          241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC
          301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG
          361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA
          421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT
          481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG
          541 GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT
          601 GTTATCCTCC TCGCCCTTGC TCACCATGGT GGATCCTATG CTAGCGGATC TGACGGTTCA
          661 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA
          721 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CNAAACAAAC
          781 TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC
          841 GCCCATTGAT GTACTGCCAA AACCGCATCA CCATNGTAAT AGCGATGANT AATACGTAGA
          901 TGTACTGCCA AGTANGAAAG TNCCNNNANN NNCATGTACT NNNCATAATG NCAGGCNGGN
          961 CNTTTACCNT CATTGACGTN ANNAGGGGGC GNANTTNNNN NATGANACAC TNNNNGNANT
        1021 GNCNANNNNN NNTTTANNNN NNNNNN
        //
        Primer: PRE_rev (Pr0250)
        Region: PRE,insert 3'(Gem1 (1-110),mVenus 3')
        Sequence file: RDB15449_A7Jvh.seq check
        >D05018A9_2_A7Jv_PRE_rev_E11_14_ABI24.ab1
            1 NNNNNNNNNN NNNNNGNNNN NGNNCNNNAT AGTTAAGAAT ACCNGTCAAT CTTTCNCAAA
           61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGATTA
          121 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT
          181 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC
          241 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT
          301 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG
          361 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC
          421 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC
          481 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC
          541 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGTAGC TCAGGTAGTG
          601 GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC
          661 GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTGGCCT TGATGCCGTT
          721 CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CCAGCTTGTG
          781 CCCCAGGATG TTGCCGTCCT CCTTGAANTC NANGCCCTTC AGCTCGATGC GNNTCACCAN
          841 GGNGTCGCCC TCNAACTTCN NNNCNGCGCG GGTCTTGNAN TTGCCGNCNN CCNNNNNNAN
        901 NNNGGNGCGC NNCNNNNNNG NNNNCNNNNN GNNNNNNNNG NNNNTNNNNN NNNNN
        //
        Primer: HIV-B_R (Pr0673)
        Region: HIV-1 psi,5' LTR,CMV pro.1
        Sequence file: RDB15449_A7Jvi.seq check
        >15449_15449_B3I4F_HIV-B_R_E03_15_ABI24.ab1
            1 NNNNNNNNNN CNNNNNAAAN TTTTGGCGTA NTCNCNAGTC GCCGCCCCTC GCCTCTTGCC
           61 GTGCGCGCTT CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT
          121 TTCAAGTCCC TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA
          181 GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG
          241 GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC
          301 AGATCTGGTC TAACCAGAGA GACCCAGTAC AGGCAAAACG CGCTGCTTAT ATAGACCTCC
          361 CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG ACATTTTGGA
          421 AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG GTGGAGACTT
          481 GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA AAACCGCATC
          541 ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA GTCCCATAAG
          601 GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC AATAGGGGGC
          661 GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG TAAATACTCC
          721 ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA CGTCATTATT
          781 GACGTCNANG GGCGGGGGTC GTNNGGNGGN CANCCAGGNG GGCCATTNAC NNNAANNNAT
        841 GNAANGCGGA NNNNCATANA NNGGNCNANN AACNNANNAN CCCNNNNANN NNNNNNNN
        //
        Primer: HIVgp_R1 (Pr0132)
        Region: HIV-1 psi,5' LTR
        Sequence file: RDB15449_A7Jvj.seq check
        >D05018A9_2_A7Jv_HIVgp_R1_A11_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNC NCTTTTTTCT CTCTGCNCCA CTCTTCTCTT TGCCTTGGTG
           61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
          121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
          181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
          241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
          301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
          361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
          421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
          481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
          541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
          601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
          661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAANGGTC TGANGGANCT
          721 CTAGTTACCA GANTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC NAGGCAAGCT
          781 TTANNGANGC TTAANCANNG GGTTNCCNAN TTANCNNGAN ANCTCCNANG NTNANNNCNN
          841 GTCNNACNNG ANAGANCNNG NACNNNNNAA ANGNNCTNNN NATATANNNN NNCNNNCNNN
        901 NNNNNCNNNN NNNCNNNTNN NNNNNNNN
        //
        Primer: CSII-CMV5' (Pr0021)
        Region: insert 5'(mCherry 3')
        Sequence file: RDB15449_A7Jvk.seq check
        >D05018A9_2_A7Jv_CSII-CMV5'_C11_08_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNGNGNNC NNGGGCGANG NNGNNAACAT GGCCATCATC
           61 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
          121 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
          181 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC
          241 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
          301 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
          361 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
          421 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG
          481 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG
          541 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG
          601 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC
          661 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG
          721 CTGTACAAGG GATATCCATC ACACTGGCGN CCGCTCGAGC CCAGCCCCGC CAGGCCCGCA
          781 CTCCGCGCCC CGGCCTCCGC TACCAGTGGC AGCCGCAAGC GCGCCCGCCC GCCCGCCGCC
          841 CCCNGGACGC NANCAGGNCA GGNNACCGGN CCGCNNNANA NNGNNGCTNN CGGNGGANNA
          901 NNTTTCCANC CCCNGTACCC CCCNANGCCC NNGANNTCCN NNNCNNNNNC NNNNNNNNGG
        961 NNNNNNNNNN
        //
        Primer: mCherry-C_F (Pr0453)
        Region: insert mid(mCherry 3',Cdt1 (30-120),P2A,mVenus 5')
        Sequence file: RDB15449_A7Jvl.seq check
        >D05018A9_2_A7Jv_mCherry-C_F_H03_08_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNGCCNGGNN CCTANNCGTC AACNTCAAGT TGGACATCAC
           61 CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC
          121 CACCGGCGGC ATGGACGAGC TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGCC
          181 CAGCCCCGCC AGGCCCGCAC TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA GCCGCAAGCG
          241 CGCCCGCCCG CCCGCCGCCC CCGGACGCGA CCAGGCCAGG CCACCGGCCC GCAGGAGACT
          301 GCGGCTGTCG GTGGACGAGG TTTCCAGCCC CAGTACCCCC GAGGCCCCAG ACATCCCAGC
          361 CTGCCCTTCT CCGGGCCAGA AGATAAAGAA ATCCACCCCG GCAGCAGGTC AGCCGCCCCA
          421 CCTGACATCC GCGCAGGACC AGGACACCAT CACCGGTCGC GCCAAGCGCG GCAGCGGCGC
          481 CACCAACTTC AGCCTGCTGA AGCAGGCCGG CGACGTGGAG GAGAACCCCG GCCCCCGCGA
          541 ATTCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
          601 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
          661 CTACGGCAAG CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
          721 CACCCTCGTG ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT
          781 GAAGCAGCAC GACTTCTTCA AGTCCGCCAN GCCCGAAGGC TACGTCCAGG NNCGCACCAT
          841 CTTCTTCAAN GANGACGGCN NCTACNAGAN CCGCGCCNAN GTGAAGTNCN NNGGCGANNC
          901 CCNNNNNAAC CNNATCNNNC TGAANGGNNT CNNNTNNNAN NNANGNANGG CNNCATCCTN
        961 NGGCNNNNNN NNGNNNNNNN NNN
        //
        Primer: EGFP-N_F (Pr0649)
        Region: insert mid(mVenus mid)
        Sequence file: RDB15449_A7Jvm.seq check
        >15449_15449_B3I4F_EGFP-N_F_F03_18_ABI24.ab1
            1 NNNNNNNNNN NNGNNNNNNC NNNGGCGNNG CNNCCTACNG CANNCTGNNC CTNAANCTGN
           61 NCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG
          121 GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG
          181 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA
          241 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG
          301 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA
          361 GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA
          421 TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC
          481 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCAAGC
          541 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG
          601 CCGGGATCAC TCTCGGCATG GACGANCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC
          661 TCGAGATGAA TCCCAGTATG AAGCAGAAAC AAGAAGAAAT CAAAGANAAT ATANAGAATA
          721 GTTCTGTCNN NAGAAGAACT CTGAAGANGA NTCAGCCTTC TGCATCTGNN TCTCTNGTTG
          781 GAAGANAAAN TGANCTGTCC GCNNNNTTGT CCAAAANGAA ACATCGGNAN TGANNACTTA
          841 ACATCTANNN NTTCCAGCCN NNGGGNTNAN NNNNCNNNNN NCNNNNNNAA NNNAAAANNN
        901 NNNNNNN
        //
        Primer: bGH_rev3 (Pr0885)
        Region: 3' LTR
        Sequence file: RDB15449_A7Jvn.seq check
        >15449_15449_B3I4F_bGH_rev3_G03_21_ABI24.ab1
            1 NNNNNNNNNN NNNNGNNGNN GGGNANNAAC ANNTGGCTGG CAACTAGANN GCACAGTCGN
           61 GGCTGNTCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC
          121 TGAGGGNTCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC
          181 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG
          241 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT CGGGCCATGT
          301 GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC GGGTCATCCA
          361 TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG GCTTCTTCTA
          421 CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT GGATATCTGA
          481 TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG TGGTAGATCC
          541 ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGANTTAG CCCTTCCAGT CCCCCCTTTT
          601 CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT AAAGGTACCT
          661 GANGTGTGAN TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG GCACAATCAG
          721 CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCNTG TTTTTCTANN TCTCGACGGT
          781 ATCNATGCNG GGNNGNNGCC CAAANGGANA TNCGANNCNN CTGANGNCGA ANNNCGAANA
        841 NNCNNNNNAN GNNNCANNNN CNNNANCNNN CCNNNNNNAN NNNNNNTCNN NNNNNNNN
        //
        Primer: SV40pro_F (Pr0110)
        Region: EM7 pro,BleoR,SV40 pA
        Sequence file: RDB15449_A7Jvo.seq check
        >D05018A9_2_A7Jv_SV40pro_F_B11_05_ABI24
            1 NNNNNNNNNN NNNNNNNNNN ANNNNTGNNG NNGCTTTTTT GGANGCCTAG GCTTTTGCNA
           61 AAAGCTCCCG GGANCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
          121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
          181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
          241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
          301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
          361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
          421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
          481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
          541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
          601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
          661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
          721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
          781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCNA CCTCTAGCTA GANCTTGGCG
          841 TAATCATGGT CATAGCTGTT TCCTGNGTGA AATTGTTATC CGCNCACAAT TCCNCACAAC
        901 ATACNANCCN GAAGCNTAAA GNNNNAAGNN NGGGGNNNNN TAANGNNNNN NNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

        2024.03.26

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