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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Dronpa/pRSETB

Photoswitchable fluorescent protein Dronpa.

Catalog number RDB15256
Resource name Dronpa/pRSETB
Clone info. Photoswitchable fluorescent protein Dronpa a.k.a. 22Gm3.
Vector backbone pRSETB (Plasmid)
Gene/insert name Echinophyllia sp. green fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Echinophyllia sp. green fluorescent protein

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15256 Dronpa/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The Dronpa/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15256).

            Reference section:

            Ando, R., Mizuno, H., Miyawaki, A., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15256_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp ()
            Region: 6xHis, T7 tag, Xpress tag, insert
            Sequence file: RDB15256_A7H9a.seq check
            >D04498G5-2_A7H9_T7_20bp_A12_03_ABI24.ab1
                1 GGGAATTCGC AGGGAATATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC
               61 TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA
              121 TCTGTACGAC GATGACGATA AGGATCCAAT GAGTGTGATT AAACCAGACA TGAAGATCAA
              181 GCTGCGTATG GAAGGCGCTG TAAATGGACA CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG
              241 GAAGCCTTTC GAGGGAAAAC AGAGTATGGA CCTTAAAGTC AAAGAAGGCG GACCTCTGCC
              301 TTTCGCCTAT GACATCTTGA CAACTGTGTT CTGTTACGGC AACAGGGTAT TCGCCAAATA
              361 CCCAGAAAAT ATAGTAGACT ATTTCAAGCA GTCGTTTCCT GAGGGCTACT CTTGGGAACG
              421 AAGCATGAAT TACGAAGACG GGGGCATTTG TAACGCGACA AACGACATAA CCCTGGATGG
              481 TGACTGTTAT ATCTATGAAA TTCGATTTGA TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT
              541 TATGCAGAAG AGGACTGTGA AATGGGAGCC ATCCACTGAG AAATTGTATG TGCGTGATGG
              601 AGTGCTGAAG GGTGATGTTA ACATGGCTCT GTCGCTTGAA GGAGGTGGCC ATTACCGATG
              661 TGACTTCAAA ACTACTTATA AAGCTAAGAA GGTTGTCCAG TTGCCAGACT ATCACTTTGT
              721 GGACCACCAC ATTGAGATTA AAAGCCACGA CAAAGATTAC AGTAATGTTA ATCTGCATGA
              781 GCATGCCGAA GCGCATTCTG AGCTGCCGAG GCAGGCCAAG TAAGAATTCG AAGCTTGATC
              841 CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC
              901 TAGCATAACC CCTTGGGGGC CTCTAAACGG GGTCTTGAGG GGGTTTTTTT GCTGAAAGGA
              961 GGAACTATAT CCCGGATCTG GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCTTTCCCA
             1021 AACAGTTGCG CAGCCTGAAA TGGGCGAAAT GGGGACGGCG CCCTGTAGCG CCGCCATTAA
             1081 GGCGCGGCGG GGTGTGGTGT TAACGCCGCA AGCCGTGCAC CGCTACCACT TGGCCAGGCG
            1141 GCCCCTTAAG G
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl