Please review the QC test results indicated by icon below as well as clone information before placing your order.

Dronpa/pRSETB

Photoswitchable fluorescent protein Dronpa.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15256_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Region: 6xHis, T7 tag, Xpress tag, insert Sequence file: RDB15256_A7H9a.seq

>D04498G5-2_A7H9_T7_20bp_A12_03_ABI24.ab1     1 GGGAATTCGC AGGGAATATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC    61 TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA   121 TCTGTACGAC GATGACGATA AGGATCCAAT GAGTGTGATT AAACCAGACA TGAAGATCAA   181 GCTGCGTATG GAAGGCGCTG TAAATGGACA CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG   241 GAAGCCTTTC GAGGGAAAAC AGAGTATGGA CCTTAAAGTC AAAGAAGGCG GACCTCTGCC   301 TTTCGCCTAT GACATCTTGA CAACTGTGTT CTGTTACGGC AACAGGGTAT TCGCCAAATA   361 CCCAGAAAAT ATAGTAGACT ATTTCAAGCA GTCGTTTCCT GAGGGCTACT CTTGGGAACG   421 AAGCATGAAT TACGAAGACG GGGGCATTTG TAACGCGACA AACGACATAA CCCTGGATGG   481 TGACTGTTAT ATCTATGAAA TTCGATTTGA TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT   541 TATGCAGAAG AGGACTGTGA AATGGGAGCC ATCCACTGAG AAATTGTATG TGCGTGATGG   601 AGTGCTGAAG GGTGATGTTA ACATGGCTCT GTCGCTTGAA GGAGGTGGCC ATTACCGATG   661 TGACTTCAAA ACTACTTATA AAGCTAAGAA GGTTGTCCAG TTGCCAGACT ATCACTTTGT   721 GGACCACCAC ATTGAGATTA AAAGCCACGA CAAAGATTAC AGTAATGTTA ATCTGCATGA   781 GCATGCCGAA GCGCATTCTG AGCTGCCGAG GCAGGCCAAG TAAGAATTCG AAGCTTGATC   841 CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC   901 TAGCATAACC CCTTGGGGGC CTCTAAACGG GGTCTTGAGG GGGTTTTTTT GCTGAAAGGA   961 GGAACTATAT CCCGGATCTG GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCTTTCCCA  1021 AACAGTTGCG CAGCCTGAAA TGGGCGAAAT GGGGACGGCG CCCTGTAGCG CCGCCATTAA  1081 GGCGCGGCGG GGTGTGGTGT TAACGCCGCA AGCCGTGCAC CGCTACCACT TGGCCAGGCG 1141 GCCCCTTAAG G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.08.13

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