Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Dronpa/pRSETB (#RDB15256)

Photoswitchable fluorescent protein Dronpa.

Clone info. Photoswitchable fluorescent protein Dronpa a.k.a. 22Gm3.
Vector backbone pRSETB (Plasmid)
Gene/insert name Echinophyllia sp. green fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考 提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15256 Dronpa/pRSETB DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Dronpa/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15256).

Reference section:

Ando, R., Mizuno, H., Miyawaki, A., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15256_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp ()
Region: 6xHis, T7 tag, Xpress tag, insert
Sequence file: RDB15256_A7H9a.seq check
>D04498G5-2_A7H9_T7_20bp_A12_03_ABI24.ab1
    1 GGGAATTCGC AGGGAATATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC
   61 TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA
  121 TCTGTACGAC GATGACGATA AGGATCCAAT GAGTGTGATT AAACCAGACA TGAAGATCAA
  181 GCTGCGTATG GAAGGCGCTG TAAATGGACA CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG
  241 GAAGCCTTTC GAGGGAAAAC AGAGTATGGA CCTTAAAGTC AAAGAAGGCG GACCTCTGCC
  301 TTTCGCCTAT GACATCTTGA CAACTGTGTT CTGTTACGGC AACAGGGTAT TCGCCAAATA
  361 CCCAGAAAAT ATAGTAGACT ATTTCAAGCA GTCGTTTCCT GAGGGCTACT CTTGGGAACG
  421 AAGCATGAAT TACGAAGACG GGGGCATTTG TAACGCGACA AACGACATAA CCCTGGATGG
  481 TGACTGTTAT ATCTATGAAA TTCGATTTGA TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT
  541 TATGCAGAAG AGGACTGTGA AATGGGAGCC ATCCACTGAG AAATTGTATG TGCGTGATGG
  601 AGTGCTGAAG GGTGATGTTA ACATGGCTCT GTCGCTTGAA GGAGGTGGCC ATTACCGATG
  661 TGACTTCAAA ACTACTTATA AAGCTAAGAA GGTTGTCCAG TTGCCAGACT ATCACTTTGT
  721 GGACCACCAC ATTGAGATTA AAAGCCACGA CAAAGATTAC AGTAATGTTA ATCTGCATGA
  781 GCATGCCGAA GCGCATTCTG AGCTGCCGAG GCAGGCCAAG TAAGAATTCG AAGCTTGATC
  841 CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC
  901 TAGCATAACC CCTTGGGGGC CTCTAAACGG GGTCTTGAGG GGGTTTTTTT GCTGAAAGGA
  961 GGAACTATAT CCCGGATCTG GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCTTTCCCA
 1021 AACAGTTGCG CAGCCTGAAA TGGGCGAAAT GGGGACGGCG CCCTGTAGCG CCGCCATTAA
 1081 GGCGCGGCGG GGTGTGGTGT TAACGCCGCA AGCCGTGCAC CGCTACCACT TGGCCAGGCG
1141 GCCCCTTAAG G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]