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Ratiometric pericam-mt/pcDNA3

Calcium ion sensor clone Ratiometric pericam-mt tagged with mitchondria localization.

Catalog number RDB15144
Resource name Ratiometric pericam-mt/pcDNA3
Clone info. Calcium ion sensor clone Ratiometric pericam-mt having a circularly permuted YFP and a CaM. Tagged with mitchondria localization signal of N-terminal 12-aa presequence of subunit IV of cytochrome c oxidase. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Schedule A (GFP-A) [Link]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please use Schedule A (GFP-A) additionally.
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        Schedule A(GFP-A, 英語版のみ) [Link]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        Schedule A(GFP-A, 英語版のみ)が追加で必要です。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15144 Ratiometric pericam-mt/pcDNA3 DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15144_A7H9p1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro,insert5'
        Sequence file: RDB15144_A7H9d.seq check
        >D04498_D2_3_A7H9_CMV-Forward_E03_15_ABI24.ab1
            1 CGAAACCCCT CCGCCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
           61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
          121 ACGACTCACT ATAGGGAGAC CCAAGCTTGC CACCATGCTG AGCCTGCGCC AGAGCATCCG
          181 CTTCTTCAAG CGCAGCGGCA TCAAGAGGCG CTGGAAGAAA AACTTCATTG CCGTCAGCGC
          241 TGCCAACCGG TTCAAGAAGA TCTCCAGCTC CGGGGCACTG GGGTCTGCAG GCTACAACAG
          301 CGACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT
          361 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC
          421 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTTCC AGTCCGCCCT
          481 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
          541 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGTCGAC GGTGGCAGCG GTGGCACCGG
          601 TGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
          661 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
          721 CAAGCTGACC CTGAAGCTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
          781 CGTGACCACC TTCGGCTACG GCCTGAAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA
          841 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
          901 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
          961 GAACCCGCAT CGAGCTGAAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCCTGGGGCA
         1021 CAAGCTGGAG TACACGGTAC CCGACCAACT GACAGGAGAG CAGGATTGCA GAGTTTCAAG
        1081 AGGCTCTCAT TAATTCGAAC AAGGATGGGG GGGACCGGGG CTATCA
        //
        Primer: BGH_rev2
        Region: bGHp(A),insert3'
        Sequence file: RDB15144_A7H9e.seq check
        >D04498_D2_3_A7H9_BGH_rev2_F03_18_ABI24.ab1
            1 GTTTCCGGGG TTAAGGAAGG CACGGGGGGG AGGGGCAAAC AAACAGATGG CTGGCAACTA
           61 GAAGGCACAG TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG
          121 CCCTCTAGAT GCATGCTCGA GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTACTT
          181 TGCTGTCATC ATTTGTACAA ACTCTTCATA GTTTACTTGG CCATCACCAT CGATATCTGC
          241 TTCCCTTATC ATTTCATCAA CTTCTTCATC TGTTAACTTC TCCCCGAGGT TTGTCATGAC
          301 GTGACGTAAC TGAGCAGCGC TGATGTAGCC GTTCCCATCC TTGTCAAAAA CACGGAATGC
          361 TTCTCGGATT TCCTCTTCGC TGTCTGTGTC CTTCATTTTT CTAGCCATCA TAGTAAGAAA
          421 TTCAGGAAAG TAAATCGTTC CATTGCCATC AGCATCGACT TCATTGATCA TATCCTGCAA
          481 TTCTGCTTCC GTTGGGTTTT GTCCAAGCGA CCTCATAACG GTGCCAAGTT CCTTTGTGGT
          541 GATGGTGCCG TCCCCATCCT TGTCGAATAA TGAGAAGGCT TCTTTGAACT CTGCAATCTG
          601 CTCTTCTGTC AGTTGGTCGG TACCGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC
          661 CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT
          721 CACCTCGGCG CGGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA
          781 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC
          841 GGGCGAAGCA CTTCAGGCCG TAGCCGATGT GGTCACGAGG GTGGGGCCAG GGCACGGGCA
          901 GCTTGCCGGT GGTGCAGATG AAGCTTTCAG GGTCAGCTTG CCGTAGTGGC ATCGCCCTCG
          961 CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAAGGAT
         1021 GGGCACCACC CCGGTGAAAC AGGCTCCTTC GCCCTTGCTT CACACCCGGG TGCCAACGGC
         1081 TGCCACCGAT CGGAACTTTG GTTACAAGCT CGTTCCATTG CCGAGGAGGT GTGAAATCCC
        1141 TGGCCGACCG
        //
        Primer: SV40pro_F
        Region: NeoR
        Sequence file: RDB15144_A7H9f.seq check
        >D04498_D2_3_A7H9_SV40pro_F_G03_21_ABI24.ab1
            1 CTCGGGTCCC CGTTGACTTT TCGGAAAGTG GTGAGGGAGG CTTTTTTGGA GGCCTAGGCT
           61 TTTGCAAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA
          121 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG
          181 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC
          241 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT
          301 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT
          361 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
          421 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC
          481 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC
          541 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG
          601 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG
          661 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT
          721 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG
          781 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA
          841 TGGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGG
          901 CCTTTCTATC GCCTTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGTTT CGAAATGACC
          961 CGACCCAAGC GACGCCCCAA CCCTGGCCAT CACGGAGATT TCCGATTTCC CACCCGCCCG
         1021 CCCTTCCTAT GGAAAGGGTT GGGGCTTTTC GGGAATTCGG TTTTTTCCCG GGGACCGCCC
         1081 GGGCTTGGGA TGGATTCCTC TCCAGCCGGC GGGGGGATTC CTTCAGTGCC TTGGAGATTT
        1141 CATTCGGCCC ACCCTCCAAC CTTATGGGTT TATTTTTGGT GACACC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.
        reference Akimzhanov, A.M., Monitoring dynamic changes in mitochondrial calcium levels during apoptosis using a genetically encoded calcium sensor. J. Vis. Exp. (50). pii: 2579 (2011). PMID 21490580.

        2022.02.09

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