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Ratiometric pericam-mt/pcDNA3

Calcium ion sensor clone Ratiometric pericam-mt tagged with mitchondria localization.

Catalog number RDB15144
Resource name Ratiometric pericam-mt/pcDNA3
Clone info. Calcium ion sensor clone Ratiometric pericam-mt having a circularly permuted YFP and a CaM. Tagged with mitchondria localization signal of N-terminal 12-aa presequence of subunit IV of cytochrome c oxidase. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |
 
Other clones in our bank X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15144 Ratiometric pericam-mt/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15144_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert5'
Sequence file: RDB15144_A7H9d.seq check
>D04498_D2_3_A7H9_CMV-Forward_E03_15_ABI24.ab1
    1 CGAAACCCCT CCGCCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTTGC CACCATGCTG AGCCTGCGCC AGAGCATCCG
  181 CTTCTTCAAG CGCAGCGGCA TCAAGAGGCG CTGGAAGAAA AACTTCATTG CCGTCAGCGC
  241 TGCCAACCGG TTCAAGAAGA TCTCCAGCTC CGGGGCACTG GGGTCTGCAG GCTACAACAG
  301 CGACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT
  361 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC
  421 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTTCC AGTCCGCCCT
  481 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
  541 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGTCGAC GGTGGCAGCG GTGGCACCGG
  601 TGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  661 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  721 CAAGCTGACC CTGAAGCTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  781 CGTGACCACC TTCGGCTACG GCCTGAAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA
  841 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  901 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  961 GAACCCGCAT CGAGCTGAAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCCTGGGGCA
 1021 CAAGCTGGAG TACACGGTAC CCGACCAACT GACAGGAGAG CAGGATTGCA GAGTTTCAAG
1081 AGGCTCTCAT TAATTCGAAC AAGGATGGGG GGGACCGGGG CTATCA
//
Primer: BGH_rev2
Region: bGHp(A),insert3'
Sequence file: RDB15144_A7H9e.seq check
>D04498_D2_3_A7H9_BGH_rev2_F03_18_ABI24.ab1
    1 GTTTCCGGGG TTAAGGAAGG CACGGGGGGG AGGGGCAAAC AAACAGATGG CTGGCAACTA
   61 GAAGGCACAG TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG
  121 CCCTCTAGAT GCATGCTCGA GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTACTT
  181 TGCTGTCATC ATTTGTACAA ACTCTTCATA GTTTACTTGG CCATCACCAT CGATATCTGC
  241 TTCCCTTATC ATTTCATCAA CTTCTTCATC TGTTAACTTC TCCCCGAGGT TTGTCATGAC
  301 GTGACGTAAC TGAGCAGCGC TGATGTAGCC GTTCCCATCC TTGTCAAAAA CACGGAATGC
  361 TTCTCGGATT TCCTCTTCGC TGTCTGTGTC CTTCATTTTT CTAGCCATCA TAGTAAGAAA
  421 TTCAGGAAAG TAAATCGTTC CATTGCCATC AGCATCGACT TCATTGATCA TATCCTGCAA
  481 TTCTGCTTCC GTTGGGTTTT GTCCAAGCGA CCTCATAACG GTGCCAAGTT CCTTTGTGGT
  541 GATGGTGCCG TCCCCATCCT TGTCGAATAA TGAGAAGGCT TCTTTGAACT CTGCAATCTG
  601 CTCTTCTGTC AGTTGGTCGG TACCGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC
  661 CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT
  721 CACCTCGGCG CGGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA
  781 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC
  841 GGGCGAAGCA CTTCAGGCCG TAGCCGATGT GGTCACGAGG GTGGGGCCAG GGCACGGGCA
  901 GCTTGCCGGT GGTGCAGATG AAGCTTTCAG GGTCAGCTTG CCGTAGTGGC ATCGCCCTCG
  961 CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAAGGAT
 1021 GGGCACCACC CCGGTGAAAC AGGCTCCTTC GCCCTTGCTT CACACCCGGG TGCCAACGGC
 1081 TGCCACCGAT CGGAACTTTG GTTACAAGCT CGTTCCATTG CCGAGGAGGT GTGAAATCCC
1141 TGGCCGACCG
//
Primer: SV40pro_F
Region: NeoR
Sequence file: RDB15144_A7H9f.seq check
>D04498_D2_3_A7H9_SV40pro_F_G03_21_ABI24.ab1
    1 CTCGGGTCCC CGTTGACTTT TCGGAAAGTG GTGAGGGAGG CTTTTTTGGA GGCCTAGGCT
   61 TTTGCAAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA
  121 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG
  181 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC
  241 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT
  301 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT
  361 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
  421 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC
  481 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC
  541 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG
  601 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG
  661 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT
  721 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG
  781 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA
  841 TGGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGG
  901 CCTTTCTATC GCCTTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGTTT CGAAATGACC
  961 CGACCCAAGC GACGCCCCAA CCCTGGCCAT CACGGAGATT TCCGATTTCC CACCCGCCCG
 1021 CCCTTCCTAT GGAAAGGGTT GGGGCTTTTC GGGAATTCGG TTTTTTCCCG GGGACCGCCC
 1081 GGGCTTGGGA TGGATTCCTC TCCAGCCGGC GGGGGGATTC CTTCAGTGCC TTGGAGATTT
1141 CATTCGGCCC ACCCTCCAAC CTTATGGGTT TATTTTTGGT GACACC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

2019.12.09

GNP_filter3_RDBDEP_html_191130.pl