Sequencing and PCR primers for primer information.
Yellow cameleon calcium ion sensor clone YCX2.60.
| Catalog number | RDB15455 |
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| Resource name | YCX2.60/pCS2 |
| Clone info. | Calcium ion sensor clone YCX2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. A new version of YC indicator with an expanded dynamic range. |
| Vector backbone | pCS2 (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA |
| Reference of nucleotide sequence | LC025957 (DDBJ accession number) |
| Depositor | Miyawaki, Atsushi | |
| Other clones in our bank | X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knopfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H. (2015) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron, 85 (5): 942-958. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word] |
| Remarks | Please obtain written approval of the depositor. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15455 | YCX2.60/pCS2 | DNA solution |
Electronic file
Featured content
| Featured content | Dr. Miyawaki's resources (English text) |
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| Featured content | Calcium Ion Sensor Clones (English text) |
References
| original | Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15455_A7Jvp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pCMV-IE94_F | RDB15455_A7Jva.seq |
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>D05018B6-2_A7Jv_pCMV-IE94_F_G02_20_ABI24.ab1 1 AGGCGGGGTG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC 61 ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC 121 TTGTTCTTTT TGCAGGATCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG 181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG 241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA 301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTGGGGCGTG CAGTGCTTCA 361 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT 421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG 481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG 541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CATCAGCCAC AACGTCTATA 601 TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG 661 AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC 721 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA 781 ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCCGC ATGCATACAG 841 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCT TCTCATTATT CGACAAGGAT GGGGGACGGC 901 ACCATCACCA CAAAGGAACT TGGCACCGTT ATGAGGTCGC TTGGACAAAA CCCAACGGAA 961 AGCAGAATTG CAGGATATGA TCAATGAAAG TCGATGCTGA TGGGCGACGG AACGATTTAC 1021 TTTTCCCTGA ATTTCTTACT ATGATGGGCT AGAAAAATGA AGTTACACAG GACCAGCCGA 1081 GGAAGGAATC GAGCAGCATC GTGCTTTTTG GACCAGGGAT GGGGAAACGG TCT // |
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| Primer: pAxCALNL_F1 | RDB15455_A7Jvb.seq |
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>D05018B6-2_A7Jv_pAxCALNLF1_H02_23_ABI24.ab1 1 CCTAATGTTT CTTTAATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GTTCTAGAGG 61 CTCGAGAGGC CTTGAATTCT TACTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC 121 CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT 181 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA 241 CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA 301 AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT 361 GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG GTGGTCACGA 421 GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT CAGCTTCAGG GTCAGCTTGC 481 CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC 541 CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA 601 TGCCACCGCT GCCACCCTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA 661 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT 721 GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT 781 GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCATGAG CTCCAGTGCC CCGGAGCTGG 841 AGGACTTCTT GAACCGGTTG GCAGCGCTGA CGGCAATGAA GTTTTTCTTC CAGCGCCTCT 901 TCCCCCCCCT TTGCTGTCAT CATTTGTACA AACTCTTCAT AGTTTACTTG GGCCATCACC 961 ATCGATATCT GCCACCCTTA TCATTTCATC AACTTTCTTC ATCTGTTAAC TTCTCCCCGG 1021 AGGTTTGTCA TGACGTGACG TAAATTTCAG CAGCGGCTGA TGTAGTCGTT TCCCATCCCT 1081 TTGTCAAAAA CACGGAATTG CATCTCGGAA TTTTCGTCGT TCGGCTTGTC GTGGGTGTAA 1141 CCC // |
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| Primer: YCX2.6_F1 | RDB15455_A7Jvc.seq |
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>D0518B6_2_A7Jv_YCX2.6_F1_H01_08_ABI08.ab1 1 AATTAAGGAA GCCTTCTCAT TATTCGACAA GGATGGGGAC GGCACCATCA CCACAAAGGA 61 ACTTGGCACC GTTATGAGGT CGCTTGGACA AAACCCAACG GAAGCAGAAT TGCAGGATAT 121 GATCAATGAA GTCGATGCTG ATGGCGACGG AACGATTTAC TTTCCTGAAT TTCTTACTAT 181 GATGGCTAGA AAAATGAAGT ACACAGACAG CGAAGAGGAA ATCCGAGAAG CATTCCGTGT 241 TTTTGACAAG GATGGGAACG GCTACATCAG CGCTGCTGAA TTACGTCACG TCATGACAAA 301 CCTCGGGGAG AAGTTAACAG ATGAAGAAGT TGATGAAATG ATAAGGGTGG CAGATATCGA 361 TGGTGATGGC CAAGTAAACT ATGAAGAGTT TGTACAAATG ATGACAGCAA AGGGGGGGAA 421 GAGGCGCTGG AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGTCCTC 481 CAGCTCCGGG GCACTGGAGC TCATGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA 541 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA 601 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT 661 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGTGGCA GCGGTGGCAT 721 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG 781 CGACGTAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 841 AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 901 GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG 961 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGGAGCGCA CCATCTTCTT 1021 TCAAGGACGA CGGGCACTAC AGACCCGCGC CGAGGGTGAA GTTCGAGGCG ACACCCTTGG 1081 TGAACCGCAT CGAGCTGAAG GCATCGACTC CAAGAGAACG CAACATCCTG GGCAACAAGC 1141 TGGGAGTTAC CACCTTTA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. |
|---|
2019.01.08
GNP_filter3_RDBDEP_html_181012.pl