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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

YCX2.60/pCS2 (#RDB15455)

Yellow cameleon calcium ion sensor clone YCX2.60.

Clone info. Calcium ion sensor clone YCX2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. A new version of YC indicator with an expanded dynamic range.
Vector backbone pCS2 (Plasmid)
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC025957 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Madisen, L. et al., Neuron 85 (5): 942-958 (2015).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Madisen, L. et al., Neuron 85 (5): 942-958 (2015).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15455 YCX2.60/pCS2 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The YCX2.60/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15455).

Reference section:

Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knöpfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15455_A7Jvp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F
Sequence file: RDB15455_A7Jva.seq check
>D05018B6-2_A7Jv_pCMV-IE94_F_G02_20_ABI24.ab1
    1 AGGCGGGGTG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC
   61 ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC
  121 TTGTTCTTTT TGCAGGATCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG
  181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG
  241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA
  301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTGGGGCGTG CAGTGCTTCA
  361 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT
  421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG
  481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG
  541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CATCAGCCAC AACGTCTATA
  601 TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG
  661 AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC
  721 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA
  781 ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCCGC ATGCATACAG
  841 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCT TCTCATTATT CGACAAGGAT GGGGGACGGC
  901 ACCATCACCA CAAAGGAACT TGGCACCGTT ATGAGGTCGC TTGGACAAAA CCCAACGGAA
  961 AGCAGAATTG CAGGATATGA TCAATGAAAG TCGATGCTGA TGGGCGACGG AACGATTTAC
 1021 TTTTCCCTGA ATTTCTTACT ATGATGGGCT AGAAAAATGA AGTTACACAG GACCAGCCGA
1081 GGAAGGAATC GAGCAGCATC GTGCTTTTTG GACCAGGGAT GGGGAAACGG TCT
//
Primer: pAxCALNL_F1
Sequence file: RDB15455_A7Jvb.seq check
>D05018B6-2_A7Jv_pAxCALNLF1_H02_23_ABI24.ab1
    1 CCTAATGTTT CTTTAATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GTTCTAGAGG
   61 CTCGAGAGGC CTTGAATTCT TACTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC
  121 CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT
  181 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA
  241 CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA
  301 AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT
  361 GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG GTGGTCACGA
  421 GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT CAGCTTCAGG GTCAGCTTGC
  481 CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC
  541 CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA
  601 TGCCACCGCT GCCACCCTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA
  661 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT
  721 GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT
  781 GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCATGAG CTCCAGTGCC CCGGAGCTGG
  841 AGGACTTCTT GAACCGGTTG GCAGCGCTGA CGGCAATGAA GTTTTTCTTC CAGCGCCTCT
  901 TCCCCCCCCT TTGCTGTCAT CATTTGTACA AACTCTTCAT AGTTTACTTG GGCCATCACC
  961 ATCGATATCT GCCACCCTTA TCATTTCATC AACTTTCTTC ATCTGTTAAC TTCTCCCCGG
 1021 AGGTTTGTCA TGACGTGACG TAAATTTCAG CAGCGGCTGA TGTAGTCGTT TCCCATCCCT
 1081 TTGTCAAAAA CACGGAATTG CATCTCGGAA TTTTCGTCGT TCGGCTTGTC GTGGGTGTAA
1141 CCC
//
Primer: YCX2.6_F1
Sequence file: RDB15455_A7Jvc.seq check
>D0518B6_2_A7Jv_YCX2.6_F1_H01_08_ABI08.ab1
    1 AATTAAGGAA GCCTTCTCAT TATTCGACAA GGATGGGGAC GGCACCATCA CCACAAAGGA
   61 ACTTGGCACC GTTATGAGGT CGCTTGGACA AAACCCAACG GAAGCAGAAT TGCAGGATAT
  121 GATCAATGAA GTCGATGCTG ATGGCGACGG AACGATTTAC TTTCCTGAAT TTCTTACTAT
  181 GATGGCTAGA AAAATGAAGT ACACAGACAG CGAAGAGGAA ATCCGAGAAG CATTCCGTGT
  241 TTTTGACAAG GATGGGAACG GCTACATCAG CGCTGCTGAA TTACGTCACG TCATGACAAA
  301 CCTCGGGGAG AAGTTAACAG ATGAAGAAGT TGATGAAATG ATAAGGGTGG CAGATATCGA
  361 TGGTGATGGC CAAGTAAACT ATGAAGAGTT TGTACAAATG ATGACAGCAA AGGGGGGGAA
  421 GAGGCGCTGG AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGTCCTC
  481 CAGCTCCGGG GCACTGGAGC TCATGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA
  541 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA
  601 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
  661 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGTGGCA GCGGTGGCAT
  721 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  781 CGACGTAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  841 AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  901 GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG
  961 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGGAGCGCA CCATCTTCTT
 1021 TCAAGGACGA CGGGCACTAC AGACCCGCGC CGAGGGTGAA GTTCGAGGCG ACACCCTTGG
 1081 TGAACCGCAT CGAGCTGAAG GCATCGACTC CAAGAGAACG CAACATCCTG GGCAACAAGC
1141 TGGGAGTTAC CACCTTTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. [link to RRC of NBRP]