RDB15455 - YCX2.60/pCS2

Catalog number	RDB15455
Resource name	YCX2.60/pCS2
Clone info.	Calcium ion sensor clone YCX2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. A new version of YC indicator with an expanded dynamic range.
Vector backbone	pCS2 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence	LC025957 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Other clones in our bank	X. laevis (KEGG orthology K02183) \| synthetic (KEGG orthology K00907) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knopfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H. (2015) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron, 85 (5): 942-958. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki

Terms and conditions set forth by the DEPOSITOR

1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knopfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H. (2015) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron, 85 (5): 942-958.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Ordering

Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment

] [Bank Transfer or Check Payment

]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word

]
Approval form [Word]

by Dr. Atsushi Miyawaki

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knopfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H. (2015) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron, 85 (5): 942-958. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15455	YCX2.60/pCS2	DNA solution

original	Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722.

Featured content	Calcium Ion Sensor Clones (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: pCMV-IE94_F Sequence file: RDB15455_A7Jva.seq
>D05018B6-2_A7Jv_pCMV-IE94_F_G02_20_ABI24.ab1 1 AGGCGGGGTG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC 61 ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC 121 TTGTTCTTTT TGCAGGATCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG 181 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG 241 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA 301 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTGGGGCGTG CAGTGCTTCA 361 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT 421 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG 481 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG 541 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CATCAGCCAC AACGTCTATA 601 TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG 661 AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC 721 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA 781 ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCCGC ATGCATACAG 841 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCT TCTCATTATT CGACAAGGAT GGGGGACGGC 901 ACCATCACCA CAAAGGAACT TGGCACCGTT ATGAGGTCGC TTGGACAAAA CCCAACGGAA 961 AGCAGAATTG CAGGATATGA TCAATGAAAG TCGATGCTGA TGGGCGACGG AACGATTTAC 1021 TTTTCCCTGA ATTTCTTACT ATGATGGGCT AGAAAAATGA AGTTACACAG GACCAGCCGA 1081 GGAAGGAATC GAGCAGCATC GTGCTTTTTG GACCAGGGAT GGGGAAACGG TCT //
Primer: pAxCALNL_F1 Sequence file: RDB15455_A7Jvb.seq
>D05018B6-2_A7Jv_pAxCALNLF1_H02_23_ABI24.ab1 1 CCTAATGTTT CTTTAATCAT GTCTGGATCT ACGTAATACG ACTCACTATA GTTCTAGAGG 61 CTCGAGAGGC CTTGAATTCT TACTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC 121 CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT 181 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA 241 CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA 301 AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT 361 GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG GTGGTCACGA 421 GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT CAGCTTCAGG GTCAGCTTGC 481 CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC 541 CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA 601 TGCCACCGCT GCCACCCTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA 661 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT 721 GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT 781 GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCATGAG CTCCAGTGCC CCGGAGCTGG 841 AGGACTTCTT GAACCGGTTG GCAGCGCTGA CGGCAATGAA GTTTTTCTTC CAGCGCCTCT 901 TCCCCCCCCT TTGCTGTCAT CATTTGTACA AACTCTTCAT AGTTTACTTG GGCCATCACC 961 ATCGATATCT GCCACCCTTA TCATTTCATC AACTTTCTTC ATCTGTTAAC TTCTCCCCGG 1021 AGGTTTGTCA TGACGTGACG TAAATTTCAG CAGCGGCTGA TGTAGTCGTT TCCCATCCCT 1081 TTGTCAAAAA CACGGAATTG CATCTCGGAA TTTTCGTCGT TCGGCTTGTC GTGGGTGTAA 1141 CCC //
Primer: YCX2.6_F1 Sequence file: RDB15455_A7Jvc.seq
>D0518B6_2_A7Jv_YCX2.6_F1_H01_08_ABI08.ab1 1 AATTAAGGAA GCCTTCTCAT TATTCGACAA GGATGGGGAC GGCACCATCA CCACAAAGGA 61 ACTTGGCACC GTTATGAGGT CGCTTGGACA AAACCCAACG GAAGCAGAAT TGCAGGATAT 121 GATCAATGAA GTCGATGCTG ATGGCGACGG AACGATTTAC TTTCCTGAAT TTCTTACTAT 181 GATGGCTAGA AAAATGAAGT ACACAGACAG CGAAGAGGAA ATCCGAGAAG CATTCCGTGT 241 TTTTGACAAG GATGGGAACG GCTACATCAG CGCTGCTGAA TTACGTCACG TCATGACAAA 301 CCTCGGGGAG AAGTTAACAG ATGAAGAAGT TGATGAAATG ATAAGGGTGG CAGATATCGA 361 TGGTGATGGC CAAGTAAACT ATGAAGAGTT TGTACAAATG ATGACAGCAA AGGGGGGGAA 421 GAGGCGCTGG AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGTCCTC 481 CAGCTCCGGG GCACTGGAGC TCATGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA 541 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA 601 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT 661 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGTGGCA GCGGTGGCAT 721 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG 781 CGACGTAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 841 AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 901 GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG 961 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGGAGCGCA CCATCTTCTT 1021 TCAAGGACGA CGGGCACTAC AGACCCGCGC CGAGGGTGAA GTTCGAGGCG ACACCCTTGG 1081 TGAACCGCAT CGAGCTGAAG GCATCGACTC CAAGAGAACG CAACATCCTG GGCAACAAGC 1141 TGGGAGTTAC CACCTTTA //