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pcDNA3-BReleaCa

Expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.

Catalog number RDB15909
Resource name pcDNA3-BReleaCa
Clone info. Expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.
UnaG(1-84)-ratCaM-UnaG(85-139).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA Rat Calm2 cDNA
Depositor Miyawaki, Atsushi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shitashima, Y. et al., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
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Remarks
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Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15909 pcDNA3-BReleaCa Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sensor and Visualization (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087.
reference Chen, T.W., Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499 (7458): 295-300 (2013). PMID 23868258.

2019.07.17

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