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pRSETB/mStayGold(c4) (#RDB20215)

Plasmid vector of mStayGold, a monomeric version of the StayGold with c4 adaptor.


Drawn by SnapGene® software
Sequence (full) RDB20215hts01.seq
Publication Ando, R., Nat. Methods 21 (4): 648-656 (2024). [link to RRC of NBRP]
Test sheet RDB20215_B3L4p1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Plasmid vector of mStayGold, a monomeric version (QC2-6 FIQ) of the StayGold. For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.
Vector backbone pRSET (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name Cytaeis uchidae GFP (mStayGold); synthetic-6xHis.
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |

Distribution information

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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., Nat. Methods 21 (4): 648-656 (2024).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., Nat. Methods 21 (4): 648-656 (2024).
備考 提供条件は英文のまま記載してください。

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RDB20215 pRSETB/mStayGold(c4) DNA solution

How to cite this biological resource

Materials & Methods section:

The pRSETB/mStayGold(c4) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20215).

Reference section:

Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [link to RRC of NBRP]