Resource data sheet
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tFucci(SA)2/pCSII-EF

Lentivirus vector construct of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15443
Resource name tFucci(SA)2/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC192886 (DDBJ accession number)
Originator Miyawaki, Atsushi |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15443 tFucci(SA)2/pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15443_A7Khp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15443_A7Kha.seq
>15443_15443_A7Kh_EF1a-C_F_A10_01_ABI24.ab1
    1 CTTTTGATTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTCTGC
  121 AGCGGCCGCG GATCCACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG
  181 GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC
  241 GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC
  301 AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC
  361 AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG
  421 GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG
  481 GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC
  541 CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG
  601 ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC
  661 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG
  721 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC
  781 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG
  841 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC
  901 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCTCGCCCC
  961 CCGGACGCGA CCAGGCCCAG GCCACTCGGG CTCCGCAGAG ACTGCGGCTG TCGGTGGGAC
 1021 GGAGATTTCA GCTCCTCAGT ACCTCCCCGA AGGCTCCTCA GGACCATCCC TAGTCCTGTC
1081 CATCTCGGAT CAAGAAGATA AGGAAATCCC AACCTCTCGG GACA
//
Primer: mCherry-C_F RDB15443_A7Khb.seq
>15443_15443_A7Kh_mCherry-C_F_B10_04_ABI24.ab1
    1 TGCAGTAAGC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
   61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA
  181 GCCCCGCCAG GCCCGCACTC CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG
  241 CCCGCCCGCC CGCCGCCCCC GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC
  301 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT
  361 GCCCTTCTCC GGGCCAGAAG ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC
  421 TGACATCCGC GCAGGACCAG GACACCATCA CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA
  481 CCAACTTCAG CCTGCTGAAG CAGGCCGGCG ACGTGGAGGA GAACCCCGGC CCCCGCGAAT
  541 TCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG
  601 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT
  661 ACGGCAAGCT GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA
  721 CCCTCGTGAC CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA
  781 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAAGGCT ACGTCCAGGA GCGCACCATC
  841 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
  901 CTGGTGAAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
  961 GGCACAAGCT GGAGTACACT ACACAGCCAC AACGTCTATA TCACGCCGAC AGCAGAGAAC
 1021 GGCCATCAGC ACTTCAGGAT TCGGCACAAC ATCGAAGACG CGCGTGCAGC TTCGCCGATC
1081 ACTACCAGCG AACACCCCTC GAATCCG
//
Primer: PRE_rev RDB15443_A7Khc.seq
>15443_15443_A7Kh_PRE_rev_C10_07_ABI24.ab1
    1 ACCGAAAACC CCAATAGGCG GTAAAAGGGA GCACATAGTT AAGAATACCA GTCAATCTTT
   61 CACAAATTTT GTAATCCAGA GGTTGATTAT CGATAAGCTT GATCCCTCGA TGTTAACTCT
  121 AGATTACAGC GCCTTTCTCC GTTTTTCTGC CACTTCCTTC CAATACTGAG AGGATGGATT
  181 TTCTTTAATC ATAAGATCAA ATGACTCCTG GGTGACTCCT CCAAGATTTT TATTTTCACT
  241 AGATTCTGGG ACAATAACCC CAGGGCTGGA AGTTGTAGAT GTTAAGTGGT CATTCCGATG
  301 TTTCCTTTTG GACAAGCCTG CGGACAGCTC ATTTTCTCTT CCAACAAGAG ATCCAGATGC
  361 AGAAGGCTGA ATCATCTTCA GAGTTCTTCT TGGGACAGAA CTATTCTTTA TATTCTCTTT
  421 GATTTCTTCT TGTTTCTGCT TCATACTGGG ATTCATCTCG AGCGGCCGCC AGTGTGATGG
  481 ATATCCCTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG
  541 CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCTTGGACT GGTAGCTCAG
  601 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG
  661 GTCGGCGAGC TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT
  721 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG
  781 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT
  841 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT
  901 GAAGAAGATG GGTGCGCTCC TGGACGTAGC CTTCGGGGCA TGGCGGGACT TGAAGAAGTC
  961 GTGCTGCTTT CATGTGGTTC GGGTAGCGGG GCGAAAGCAC TGGCAGGCCG GTAGCCCAGG
 1021 GTGGTCACGA AGGGGTGGGC CCAGGGCACG GGGCAGCTTT GCCGGGTGGA TGGCAGATCA
 1081 GCCTTTCAGG GTTCAGGCCT TGGCCCGGTA AGGTTGGCCA ATCCGCCTTC GTCCTTCGTC
1141 GGACCGCTGA ACCTGGTGGC GCGCGT
//
Primer: CMV-Forward RDB15443_A7Khd.seq
//
Primer: BGH_rev2 RDB15443_A7Khe.seq
//
Primer: SV40pro_F RDB15443_A7Khf.seq
//
Primer: EF1a-N_R RDB15443_A7Khg.seq
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2019.04.03

GNP_filter3_RDBDEP_html_190327.pl