Resource data sheet
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tFucci(SA)2/pCSII-EF

Lentivirus vector construct of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15443
Resource name tFucci(SA)2/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC192886 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15443_A7Jvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15443_A7Jva.seq
>D05018A3_A7Jv_2_EF1a-C_F_F01_16_ABI24.ab1
    1 ACCTTTTGGA TTGCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCT
  121 GCAGCGGCCG CGGATCCACC ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA
  181 AGGAGTTCAT GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA
  241 TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA
  301 CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT
  361 CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG
  421 AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC
  481 AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT
  541 TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC
  601 GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG
  661 ACGGCGGCCA CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC
  721 TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA
  781 CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAGC
  841 TGTACAAGGG ATATCCATCA CACTGGCGGC CGCTCGAGCC CAGCCCCGCC AGGCCCGCAC
  901 TCCGCGCCCC GGCCTCCGCT ACCAGTGGCA GCCGCAAGCG CGCCCGCCCG CCCGCCGCCC
  961 CCGGACGCGA CCAGGCCCAG GGCCACCGGG CCCGCAGAGA CTGCGGCTGT CGGTGGACGA
 1021 AGTTTCCAGC CCCAGTACCC CCGAGCCCCA GACATCCCAG CCTGGCCTTC TCGGATCAGA
1081 GATAAAGAAA TTCACCCGCC AGCCAAGTCA GGCGACCCCA CCCTTGAAAC ATTCCGTCG
//
Primer: mCherry-C_F RDB15443_A7Jvb.seq
>D05018A3_2_A7Jv_mCherry-C_F_F01_06_ABI08.ab1
    1 GAACAGGAGA CCCGGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA
   61 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
  121 CCACCGGCGG CATGGACGAG CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGC
  181 CCAGCCCCGC CAGGCCCGCA CTCCGCGCCC CGGCCTCCGC TACCAGTGGC AGCCGCAAGC
  241 GCGCCCGCCC GCCCGCCGCC CCCGGACGCG ACCAGGCCAG GCCACCGGCC CGCAGGAGAC
  301 TGCGGCTGTC GGTGGACGAG GTTTCCAGCC CCAGTACCCC CGAGGCCCCA GACATCCCAG
  361 CCTGCCCTTC TCCGGGCCAG AAGATAAAGA AATCCACCCC GGCAGCAGGT CAGCCGCCCC
  421 ACCTGACATC CGCGCAGGAC CAGGACACCA TCACCGGTCG CGCCAAGCGC GGCAGCGGCG
  481 CCACCAACTT CAGCCTGCTG AAGCAGGCCG GCGACGTGGA GGAGAACCCC GGCCCCCGCG
  541 AATTCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
  601 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA
  661 CCTACGGCAA GCTGACCCTG AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC
  721 CCACCCTCGT GACCACCCTG GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA
  781 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA
  841 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA
  901 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG
  961 GGCACAAGCT GGGAGTACAA CTACAACAGC CACAACGTCT ATATCACCGC CGACAAGCAG
 1021 AGAACGGCAT CAAGGCCAAC TTCAAGATCC GCCACAACAT CGAGGACGGG CGGCGTGCAG
 1081 CTTCGTCGAC ACTACCAGCA GAACATCCCT CAATCGGCGA ACGGCCTCGC TGCCTGCCTG
1141 CTCGAACAAC TCACTTACCC TGTGAGAGCT C
//
Primer: PRE_rev RDB15443_A7Jvc.seq
>D05018A3_A7Jv_2_PRE_rev_G01_19_ABI24.ab1
    1 AGGTATCAAC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
  121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
  181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
  241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
  301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
  361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
  421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
  481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
  541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGTAG CTCAGGTAGT
  601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG
  661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT
  721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT
  781 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
  841 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA
  901 AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT
  961 TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCAGGCCGTA GCCCAGGGTG GGTCACGAGG
 1021 GTGGGCCAGG GCACGGGCAG CTTTGCCCGG TGGTGCAGAT CAGCTTCAGG GGTTCAGCTT
 1081 TGCCGTAGGT GGCATCGACC TCGACTTCGG CCGACACGGC TGAACTTGGT GGCCGATTTA
1141 ACGGTTCCGC CCCGGTTCAG C
//
Primer: CMV-Forward RDB15443_A7Jvd.seq
>D05018A3_2_A7Jv_CMV-Forward_H02_08_ABI08.ab1
    1 CTTTAAACCT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
  121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
  181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
  241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
  301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
  361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
  421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
  481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
  541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
  601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
  661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
  721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
  781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGGACAAT TGGAGAAGTG AATTATATAA
  841 ATATAAAGTA GTAAAAATTG AAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAAGA
  901 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA AATAGGAGCT TTGTTCCCTT GGGTTCTTGG
  961 GAGCAGCAGG AAAGCACTAT GGGGCGCAGC GTCAATGACG CTGACGGGTA CAGGGCCCAG
 1021 ACATTTATTT GTCTGGGTAT AGTTGCAAGC AGCAGACAAA TTTTGCCTGA GGCTATTGAG
 1081 GCGCCACCAG CATCCTGTTG GCAACTTCAC AGTCTGGGGG CAATCCAGGC AAGCCTCCAA
1141 GGCAGGAAGT CTGTGTGGGC TTGTGTTGGA
//
Primer: BGH_rev2 RDB15443_A7Jve.seq
>D05018A3_A7Jv_2_BGH_rev2_A02_02_ABI24.ab1
    1 CCAGTTAGGA TCAGAGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
  301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
  361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
  421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
  481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
  541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
  601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
  661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
  721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
  781 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
  841 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
  901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
  961 GCGAGCAGCC ATGGAAAAGG ACGTCAGCTT TCCCCGACAC ACCACGGGAA TTGTCAGTGC
 1021 CCACAGCCGA GCCCCTGTCC AGCAGCGGGC AGCAGCGCGA TGAGGTTCGG TCGTGCATAG
 1081 GAGGGGGAAA GCGAAGGTCC GCAAGAGCCT GACAGTGTTG TATGCCTCCT AACTCAGTGA
1141 G
//
Primer: SV40pro_F RDB15443_A7Jvf.seq
>D05018A3_A7Jv_2_SV40pro_F_B02_05_ABI24.ab1
    1 ACCTTGTCCT GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCCAGCTGC
 1021 ATTAATGAAA TCGGGCCAAC GCGCGGGGAG AGCGGGTTTT GCGTATTGGG CGCTCTTCGG
 1081 CTTTCTCGCT CACTGAACTT CGCTTGCGCC TCGATTCGAT CCGGGCCTGG CGGACGAGCG
1141 GGTAATTCCA GGCCTTCAAC T
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2018.03.15

GNP_filter3_RDBDEP_html_171214.pl