Resource data sheet
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tFucci(SA)2/pCSII-EF

Lentivirus vector construct of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15443
Resource name tFucci(SA)2/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC192886 (DDBJ accession number)
Originator Miyawaki, Atsushi |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
Order form 
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15443 tFucci(SA)2/pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15443_A7Jvp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: EF-1a pro,insert5' | Primer: EF1a-C_F | Sequence: RDB15443_A7Jva.seq
>D05018A3_2_A7Jv_CMV-Forward_H02_08_ABI08.ab1
    1 CTTTAAACCT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
  121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
  181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
  241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
  301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
  361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
  421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
  481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
  541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
  601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
  661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
  721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
  781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGGACAAT TGGAGAAGTG AATTATATAA
  841 ATATAAAGTA GTAAAAATTG AAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAAGA
  901 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA AATAGGAGCT TTGTTCCCTT GGGTTCTTGG
  961 GAGCAGCAGG AAAGCACTAT GGGGCGCAGC GTCAATGACG CTGACGGGTA CAGGGCCCAG
 1021 ACATTTATTT GTCTGGGTAT AGTTGCAAGC AGCAGACAAA TTTTGCCTGA GGCTATTGAG
 1081 GCGCCACCAG CATCCTGTTG GCAACTTCAC AGTCTGGGGG CAATCCAGGC AAGCCTCCAA
1141 GGCAGGAAGT CTGTGTGGGC TTGTGTTGGA
//
Region: insert(mid) | Primer: mCherry-C_F | Sequence: RDB15443_A7Jvb.seq
>D05018A3_A7Jv_2_BGH_rev2_A02_02_ABI24.ab1
    1 CCAGTTAGGA TCAGAGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
  301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
  361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
  421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
  481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
  541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
  601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
  661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
  721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
  781 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
  841 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
  901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
  961 GCGAGCAGCC ATGGAAAAGG ACGTCAGCTT TCCCCGACAC ACCACGGGAA TTGTCAGTGC
 1021 CCACAGCCGA GCCCCTGTCC AGCAGCGGGC AGCAGCGCGA TGAGGTTCGG TCGTGCATAG
 1081 GAGGGGGAAA GCGAAGGTCC GCAAGAGCCT GACAGTGTTG TATGCCTCCT AACTCAGTGA
1141 G
//
Region: PRE,insert3' | Primer: PRE_rev | Sequence: RDB15443_A7Jvc.seq
>D05018A3_A7Jv_2_SV40pro_F_B02_05_ABI24.ab1
    1 ACCTTGTCCT GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCCAGCTGC
 1021 ATTAATGAAA TCGGGCCAAC GCGCGGGGAG AGCGGGTTTT GCGTATTGGG CGCTCTTCGG
 1081 CTTTCTCGCT CACTGAACTT CGCTTGCGCC TCGATTCGAT CCGGGCCTGG CGGACGAGCG
1141 GGTAATTCCA GGCCTTCAAC T
//
Region: CMVpro,5'LTR,HIV-1psi | Primer: CMV_Forward | Sequence: RDB15443_A7Jvd.seq
>D05018A3_2_A7Jv_CMV-Forward_H02_08_ABI08.ab1
    1 CTTTAAACCT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
  121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
  181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
  241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
  301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
  361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
  421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
  481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
  541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
  601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
  661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
  721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
  781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGGACAAT TGGAGAAGTG AATTATATAA
  841 ATATAAAGTA GTAAAAATTG AAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAAGA
  901 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA AATAGGAGCT TTGTTCCCTT GGGTTCTTGG
  961 GAGCAGCAGG AAAGCACTAT GGGGCGCAGC GTCAATGACG CTGACGGGTA CAGGGCCCAG
 1021 ACATTTATTT GTCTGGGTAT AGTTGCAAGC AGCAGACAAA TTTTGCCTGA GGCTATTGAG
 1081 GCGCCACCAG CATCCTGTTG GCAACTTCAC AGTCTGGGGG CAATCCAGGC AAGCCTCCAA
1141 GGCAGGAAGT CTGTGTGGGC TTGTGTTGGA
//
Region: SV40pro_ori,EM7pro,BleoR,SV40p(A) | Primer: SV40pro_F | Sequence: RDB15443_A7Jvg.seq
>15443_15443_A7Jv_EF1a-N_R_A02_02_ABI24.ab1
    1 CGGAGGGGCT GTGGGCGATG TGCGCTCTGC CCACTGACGG GCACCGGAGC CTCACGAAGC
   61 TTGAATTAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA AACCCGAAAA TTTTGAATTT
  121 TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT TGCTATTATG TCTACTATTC
  181 TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT AAAATTGTGG ATGAATACTG
  241 CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC TGTCTCTCTC TCCACCTTCT
  301 TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG GGAGGTGGGT CTGAAACGAT
  361 AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA GTACAGCAAA AACTATTCTT
  421 AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA TATACCACAG CCAATTTGTT
  481 ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT CCAATAATTC TTGTTCATTC
  541 TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAGGAGTGTA TTAAGCTTGT GTAATTGTTA
  601 ATTTCTCTGT CCCACTCCAT CCAGGTCGTG TGATTCCAAA TCTGTTCCAG AGATTTATTA
  661 CTCCAACTAG CATTCCAAGG CACAGCAGTG GTGCAAATGA GTTTTCCAGA GCAACCCCAA
  721 ATCCCCAGGA GCTGTTGATC CTTTAGGTAT CTTTCCACAG CCAGGATTCT TGCCTGGAGC
  781 TGCTTGATGC CCCAGACTGT GAGTTGCAAC AGATGCTGTT GCGCCTCAAT AGCCCTCAGC
  841 AAATTGTTCT GCTGCTGCAC TATACCAGAC AATAATTGTC TGGCCTGTAC CGTCAGCGTC
  901 ATTGACGCTG CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAGGAA ACAAAGCTCC
  961 TATTTCCCAC TGCTCTTTTT TTTCTCTCTG CACCACTCTT CTCTTTTGCC CTTTGGTGGG
 1021 TGCCTACTCC TAATGGATTC AATTTTTTAC CTACCTCATA GTGATAATAA TCACTTCTCG
1081 AACTGGTCCT TCAATATTCT CGTTCGATCA GGCTTCCTTG CAGGAT
//
Region: EF-1a pro,cPPT_CTS,RRE | Primer: EF1a-N_R | Sequence: RDB15443_A7Jve.seq
>D05018A3_A7Jv_2_BGH_rev2_A02_02_ABI24.ab1
    1 CCAGTTAGGA TCAGAGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
  301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
  361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
  421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
  481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
  541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
  601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
  661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
  721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
  781 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
  841 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
  901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
  961 GCGAGCAGCC ATGGAAAAGG ACGTCAGCTT TCCCCGACAC ACCACGGGAA TTGTCAGTGC
 1021 CCACAGCCGA GCCCCTGTCC AGCAGCGGGC AGCAGCGCGA TGAGGTTCGG TCGTGCATAG
 1081 GAGGGGGAAA GCGAAGGTCC GCAAGAGCCT GACAGTGTTG TATGCCTCCT AACTCAGTGA
1141 G
//
Region: bGHp(A),3'LTR,PRE | Primer: BGH_rev2 | Sequence: RDB15443_A7Jvf.seq
>D05018A3_A7Jv_2_SV40pro_F_B02_05_ABI24.ab1
    1 ACCTTGTCCT GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCCAGCTGC
 1021 ATTAATGAAA TCGGGCCAAC GCGCGGGGAG AGCGGGTTTT GCGTATTGGG CGCTCTTCGG
 1081 CTTTCTCGCT CACTGAACTT CGCTTGCGCC TCGATTCGAT CCGGGCCTGG CGGACGAGCG
1141 GGTAATTCCA GGCCTTCAAC T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2019.10.08

GNP_filter3_RDBDEP_html_190927.pl