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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(SA)2/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15443
Resource name tFucci(SA)2/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC192886 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15443 tFucci(SA)2/pCSII-EF DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15443_A7Jvp1-5.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EF1a-C_F
        Region: EF-1 alpha pro,insert 5'(mCherry 5')
        Sequence file: RDB15443_A7Jvh.seq check
        >D05018A3_A7Jv_2_EF1a-C_F_F01_16_ABI24.ab1
            1 NNNNNNNNNN NNNNCNTTTT GAGTTTGGNN CTTGGTTCNT TCTCAAGCCT CAGACAGTGG
           61 TTCAAAGTTT TTTTCTTCCN TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCT
          121 GCAGCGGCCG CGGATCCACC ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA
          181 AGGAGTTCAT GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA
          241 TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA
          301 CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT
          361 CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG
          421 AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC
          481 AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT
          541 TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC
          601 GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG
          661 ACGGCGGCCA CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC
          721 TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA
          781 CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC CNCCGGCGGC ATGGACGANC
          841 TGTACAAGGG ATATCCATCA CACNGGCGGC CGCNNNANCC CAGCCCCGNC ANGCCCGCAC
          901 TCCGCGCCCC GGNCNNCGNN ACCANNGGCA GCCGCAAGCG CGCCCGCCCG NNCNNNGCCC
        961 CCNGNNNNNN NNNN
        //
        Primer: PRE_rev
        Region: PRE,insert 3'(hGem(1/110),mVenus 3')
        Sequence file: RDB15443_A7Jvi.seq check
        >D05018A3_A7Jv_2_PRE_rev_G01_19_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NGGAGCNNCA TAGTTAAGAA TACCAGTCAA TCTTTCNCAA
           61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
          121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
          181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
          241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
          301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
          361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
          421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
          481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
          541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGTAG CTCAGGTAGT
          601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG
          661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT
          721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT
          781 GCCNCANGNT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
          841 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGNCTTGTA GTTGNCGTCG NCCTTGAANA
          901 AGANGGTGCG CTCCNNGANG TAGCCNNCNG GNATNGCGGA NTTNAANAAN TCGNGCTGCT
          961 TCATGNNGNN GGGGTANCGG GCGANNCACT GNNNNNNGTA GCCNANGNNN GNNCNNNNNG
        1021 NNNGNNNNNG NNNNNNNNNN
        //
        Primer: mCherry-C_F
        Region: insert mid(mCherry 3',hCdt1(30/120),P2A,mVenus 5')
        Sequence file: RDB15443_A7Jvj.seq check
        >D05018A3_2_A7Jv_mCherry-C_F_F01_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NTGCNNNNNN NNNNNNNNNT CNACATCAAG TTGGANATCA
           61 CCTCCCACAA CGAGGACTAC NNCNTCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
          121 CCACCGGCGG CATGGACGAG CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGC
          181 CCAGCCCCGC CAGGCCCGCA CTCCGCGCCC CGGCCTCCGC TACCAGTGGC AGCCGCAAGC
          241 GCGCCCGCCC GCCCGCCGCC CCCGGACGCG ACCAGGCCAG GCCACCGGCC CGCAGGAGAC
          301 TGCGGCTGTC GGTGGACGAG GTTTCCAGCC CCAGTACCCC CGAGGCCCCA GACATCCCAG
          361 CCTGCCCTTC TCCGGGCCAG AAGATAAAGA AATCCACCCC GGCAGCAGGT CAGCCGCCCC
          421 ACCTGACATC CGCGCAGGAC CAGGACACCA TCACCGGTCG CGCCAAGCGC GGCAGCGGCG
          481 CCACCAACTT CAGCCTGCTG AAGCAGGCCG GCGACGTGGA GGAGAACCCC GGCCCCCGCG
          541 AATTCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
          601 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA
          661 CCTACGGCAA GCTGACCCTG AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGNC
          721 CCACCCTCGT GACCACCCTG GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA
          781 TGAAGCAGCA CGACTTCTTC NAGTCCNCCA TGCCCGANNG CTACGTCCAG GANCGCACCA
          841 TCTTCTTCAA NGANGANGGC NNNTACAAGA CCCGCGCCGA NGNGANNTNN GAGGGCGANA
          901 CCCTGGTGAA NCGCATCGAG NTNNNNGGCA TNGACTTNNN GGANGANNGN AACATNCTGG
        961 GGNANNNGCN NGNANNANNN CTACNNNANN NNCNNCNNCT ATATNACNNN NNNNNNN
        //
        Primer: EGFP-N_F
        Region: inser mid(mVenus 3',hGem(1/110) 5')
        Sequence file: RDB15443_A7Jvk.seq check
        >15443_15443_B2FgF_EGFP-N_F_C01_03_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNNGCNNC CTACGGCAAG CTGNNCCTGA
           61 AGCTGATCTG CACCACCGGN AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
          121 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
          181 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
          241 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
          301 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
          361 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT
          421 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA
          481 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
          541 CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
          601 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GGGATATCCA TCACACTGGC
          661 GGCCGCTCGA GATGAATCCC AGTATGAAGC AGAAACAAGA AGAAATCAAA GAGAATATAA
          721 AGAATAGTTC TGTCCCAAGA AGAACTCTGA AGANGANTCA GCCTTCTGCA TCTGGATCTC
          781 TTGTTGGAAG AGAAAATGAG CTGTCCGCAN GCTTGTCNNA AAGGAAACAT CGGAATGANC
          841 ACTTAACATC TACAACTTCC AGCCCTGGGG TTATTGTCCC AGAATCTANT GAAAATAAAA
          901 ATCTTGGANG ANTCANCCNG GANNCATTTG ATCTTATGAN NAAAGAAAAT CCNTCCTCTC
        961 ANNNNNNNNN N
        //
        Primer: CMV_Forward
        Region: CMV pro,5' LTR,HIV-1psi
        Sequence file: RDB15443_A7Jvl.seq check
        >D05018A3_2_A7Jv_CMV-Forward_H02_08_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNATG GGCGGTAGGC GTGTNCGGTG GGAGGTCTAT
           61 ATAAGCAGCG CGTTNNNNNN NTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
          121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
          181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
          241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
          301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
          361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
          421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
          481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
          541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
          601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTNNN NNNATTATAT
          661 AATANNNNNN NNNCCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
          721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
          781 CTGATCTTCA NACCTGGANG ANGANATATG NNGGGANAAT TGGAGAAGTG AATTATATAA
          841 ATATAAAGTA GTAAAANTTG NANNCATTAN GANTANCNCC CNNCAAGGNN AAGAGAANNA
          901 GTGGTGCAGA NNNAAAAAAN ANCAGTGNGN ANTNNGANCT TTGTTNCNTN GGNNNNTTGG
          961 GANCAGCNNG NAANCACTNN NGGGCNCAGC GNNNNTGANN CNNNNNGGTN NNNGNNCNNN
        1021 NNNNTNANTN GNNNNGGNAT ANNNNNNNNN N
        //
        Primer: EF1a-N_R
        Region: EF-1 alpha pro,cTTP_CTS,RRE
        Sequence file: RDB15443_A7Jvm.seq check
        >15443_15443_A7Jv_EF1a-N_R_A02_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNG GCGNNGTGCG CTCTGCCCNC TGNCGGGCAC CGGNNCCTCA
           61 CGAAGCTTGA ATTAATTCAA AGTGAATTTC TGCTGTCCCT GTAATAAACC CGAAAATTTT
          121 GAATTTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA
          181 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA
          241 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA
          301 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA
          361 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT
          421 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA
          481 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT
          541 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA
          601 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT
          661 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA
          721 CCCCAAATCC CCAGGANCTG TTGATCCTTT ANGTATCTTT CCACAGCCAG GNNTCTTGCC
          781 TGGANCTGCT TGATGCNNCA GANTGTGAGT TGCAACAGAT GCTGTTGCGC CTCNATANCC
          841 CTCANCAAAN TGNNCTGCTG CTGCACTATA CCNNANNATA ATTGNCNNGN CNGNANNGNC
          901 ANCNNCNNTN NNGCNNCGCC CATANNGNNN NNNGCTGNNN CNNNNNNNCN NNNNNNNNAN
        961 NNNNCNNNNT NNNNNNNNNN
        //
        Primer: BGH_rev2
        Region: bGH pA,3' LTR
        Sequence file: RDB15443_A7Jvn.seq check
        >D05018A3_A7Jv_2_BGH_rev2_A02_02_ABI24.ab1
            1 NNNNNNNNNN NNNGNNNNNG GNGGNNGGGC NNACAACAGA TGGCTGGCAA CTAGAAGGCA
           61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
          121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
          181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
          241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
          301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
          361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
          421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
          481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
          541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
          601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
          661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
          721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCNTGTTT TTCTAGGTCT
          781 CGACGGTATC GATGCGGGGN NGCGGCCCNA AGGGANATCN GANTCGTCTG ANGGCGAANG
          841 CGAAGANGCG GAANANGCCG CANANNCNGC AGCANGNNGC GGGAANGAAN GTCCGCNGGA
          901 TTNAGGGCCG AAGGGNNGNA NCANANNNAN NTCCCGCGCA NNNNCCNNGN NGNNNCNCNN
        961 GCNANCAGNN NNNGNAANNG NNNNCNNCNT NNCNNNNNNN NNN
        //
        Primer: SV40pro_F
        Region: SV40pro_ori,EM7 pro,Zeo,SV40 pA
        Sequence file: RDB15443_A7Jvo.seq check
        >D05018A3_A7Jv_2_SV40pro_F_B02_05_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN ANNNNTGANG ANGNTTTTTT GGAGGCCTAG GCTTTTGCAA
           61 AAAGCTCCCG GGAGCTTGTA TATCCNTTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
          121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
          181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
          241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
          301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
          361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
          421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
          481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
          541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
          601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
          661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
          721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
          781 NAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
          841 TAATCATGGT CATAGCTGTT NNNNNNNGTG AAATTGTTAT CNGCTCACAA TTNNNCACNA
          901 CATACGAGCC GGAAGCATAA AGNGNAAAGC CNGGGGNGNC NNNNGANNGA NCNNACNCNC
        961 ATTANNTGCG NNGNNNNNNC NGNCNNCNTT CCANNNNGNA AANNNNNNNN NN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.07.01

        GNP_filter3_RDBDEP_html_220520.pl