Please review the QC test results indicated by icon below as well as clone information before placing your order.

mCherry-hCdt1(1/100)Cy(-)/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-). EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions for distribution	The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
付加的提供条件	レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15441_A7Jvp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB15441_A7Jva.seq
>D05018A1_1_A7Jv_EF1a-C_F_D03_04_ABI08.ab1     1 CTGGGGGGGA TTGGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT    61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT   121 CGCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG   181 CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG   241 CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC   301 CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT   361 GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG   421 GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT   481 GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG   541 CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA   601 GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA   661 CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA   721 CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA   781 GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGGGATA   841 TCCATCACAC TGGCGGCCGC TCGAGATGGA GCAGCGCCGC GTCACCGACT TCTTCGCGCG   901 CCGCCGCCCC GGGCCCCCCC CGCATCGCGC CGCCCAAGCT GGGCCTGCCG CACCCCCCAG   961 CCCCGCCAGG GCCCGCACTC CGCGCCCCGG GCCCTCCGCT ACCAGTGGCA GCCGCAAGCG  1021 CGCCCGCCCG GCCCGCCGCC CCCGGGACGC GAACAGTCAA GGTCACCGGG CCCGCGTCGA 1081 CGCGCGACTG TCGGTGGAAC GAAGGTTTCC AAGGTCTCA //
Primer: PRE_rev Sequence file: RDB15441_A7Jvb.seq
>D05018A1_1_A7Jv_PRE_rev_E10_13_ABI24.ab1     1 ACGGTACCAA AATAGGCGGT AAGGAGCACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT   121 ATTTCTTTAT CTTCTGGCCC GGAGAAGGGC AGGCTGGGAT GTCTGGGGCC TCGGGGGTAC   181 TGGGGCTGGA AACCTCGTCC ACCGACAGCC GCGCGGCCGC GCGGGCCGGT GGCCTGGCCT   241 GGTCGCGTCC GGGGGCGGCG GGCGGGCGGG CGCGCTTGCG GCTGCCACTG GTAGCGGAGG   301 CCGGGGCGCG GAGTGCGGGC CTGGCGGGGC TGGGGGTGCG GCAGGCCAGC TTGGGCGGCG   361 CGATGCGGGG GGGCCCGGGG CGGCGGCGCG CGAAGAAGTC GGTGACGCGG CGCTGCTCCA   421 TCTCGAGCGG CCGCCAGTGT GATGGATATC CCTTGTACAG CTCGTCCATG CCGCCGGTGG   481 AGTGGCGGCC CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAGG   541 TGATGTCCAA CTTGATGTTG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT   601 TGTAGGTGGT CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT   661 TGATCTCGCC CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCGGAGGAG GCCTCCCAGC   721 CCATGGTCTT CTTCTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA   781 CCTTGTAGAT GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC   841 CGTCCTCGAA GTTCATCACG CGCTCCCACT TGAAGCCCTC GGGGAAAGGA CAGCTTCAAG   901 TAGTCGGGGA TGTCGGCGGG GGTGCTTCAC GTAGGCCCTT GGAGCCCGTA CATGAACTGA   961 GGGGACAGGA TGTCCCAAGG CGGAGGGCAG GGGGCCACCC TTTGGTTCAA CCTTTCAGCT  1021 TTGACCGGAT CTGGGATGCC TTCGTTAGGA CGGATCCTCG ACCTCGCCTC GATCTCGAAC 1081 TCGATGAGCC //
Primer: CMV_Forward Sequence file: RDB15441_A7Jvc.seq
>D05018A1_1_A7Jv_CMV-Forward_E03_05_ABI08.ab1     1 CTGATCCCTT TCCGGCCCCC ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG   121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG   181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC   241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG   301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG   361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA   421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA   481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA   541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC   601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT   661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG   721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGGGC   781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA   841 AATATAAAGT AGTAAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA   901 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA AATAGGAGCT TTTGTTCCTT GGGGTTCTTG   961 GGGAGCAGCA GGAAGGCACT AATGGGGCGC AGCGTCAATG ACGGCCTGAC GGTACAGGCC  1021 AGACAATTTA TGGTTCTGGG TATAGGTTGC AGCCAGCCAA GAACCAATTT GCTGGGAGAG 1081 GGACCTAATG //
Primer: BGH_rev Sequence file: RDB15441_A7Jvd.seq
>D05018A1_1_A7Jv_BGH_rev_F10_16_ABI24.ab1     1 GTGTCGGGGG TCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT    61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT   121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA   181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC   241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA   301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA   361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG   421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT   481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC   541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA   601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG   661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG   721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA   781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC   841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGGAAAGGGA   901 CGTCAGCTTC CCCGACACAC CCACGGGAAT TGTCAGTGCC CAAACAGCCG AGCCCCTGTC   961 CAGCAGCGGG CAGCAGGCGC GATGAGTTCG GCGGTGCATA GGGAGGGGGA AAGCGAAGTT  1021 CCCGAAAGAA GCTGGACAGT GATGGCCATG GCCTCATCAA GTGGATGCGT CAGTACACAG 1081 TGCACACCCA CGTCTACGAT //
Primer: SV40pro_F Sequence file: RDB15441_A7Jve.seq
>D05018A1_1_A7Jv_SV40pro_F_F03_06_ABI08.ab1     1 CCTCGTTCCG TTGGGAATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG   781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG   841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC   901 AACATACGAG CCCGGAAGCA TAAAGTGTAA AGCCTGGGGG TGCCTAATGA GTGAGCTAAC   961 TCACATTAAA TTGCGTTGCG CTCACTGCCC CGCTTTCCAG TCGGGAAACC CTGGTCGTGC  1021 CAGCCTGCAA TTAATGAATC GGGCCAACGC CGCGGGGAGA GCGGTTTGCC GTAATGGGGC 1081 GCTTCTTCCG GCCTTCCCTC GCCTTCACAC CTGTGGAACC TTTCTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl