Resource data sheet
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mCherry-hCdt1(1/100)Cy(-)/pCSII-EF

Lentivirus expression vector of mCherry-hCdt1(1/100)Cy(-). EF-1 alpha promoter.

Catalog number RDB15441
Resource name mCherry-hCdt1(1/100)Cy(-)/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(1/100)Cy(-). EF-1 alpha promoter.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of nucleotide sequence LC171208 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15441_A7Jvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15441_A7Jva.seq
>D05018A1_1_A7Jv_EF1a-C_F_D03_04_ABI08.ab1
    1 CTGGGGGGGA TTGGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
   61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT
  121 CGCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG
  181 CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG
  241 CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC
  301 CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT
  361 GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG
  421 GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT
  481 GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG
  541 CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA
  601 GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA
  661 CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA
  721 CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA
  781 GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGGGATA
  841 TCCATCACAC TGGCGGCCGC TCGAGATGGA GCAGCGCCGC GTCACCGACT TCTTCGCGCG
  901 CCGCCGCCCC GGGCCCCCCC CGCATCGCGC CGCCCAAGCT GGGCCTGCCG CACCCCCCAG
  961 CCCCGCCAGG GCCCGCACTC CGCGCCCCGG GCCCTCCGCT ACCAGTGGCA GCCGCAAGCG
 1021 CGCCCGCCCG GCCCGCCGCC CCCGGGACGC GAACAGTCAA GGTCACCGGG CCCGCGTCGA
1081 CGCGCGACTG TCGGTGGAAC GAAGGTTTCC AAGGTCTCA
//
Primer: PRE_rev RDB15441_A7Jvb.seq
>D05018A1_1_A7Jv_PRE_rev_E10_13_ABI24.ab1
    1 ACGGTACCAA AATAGGCGGT AAGGAGCACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
  121 ATTTCTTTAT CTTCTGGCCC GGAGAAGGGC AGGCTGGGAT GTCTGGGGCC TCGGGGGTAC
  181 TGGGGCTGGA AACCTCGTCC ACCGACAGCC GCGCGGCCGC GCGGGCCGGT GGCCTGGCCT
  241 GGTCGCGTCC GGGGGCGGCG GGCGGGCGGG CGCGCTTGCG GCTGCCACTG GTAGCGGAGG
  301 CCGGGGCGCG GAGTGCGGGC CTGGCGGGGC TGGGGGTGCG GCAGGCCAGC TTGGGCGGCG
  361 CGATGCGGGG GGGCCCGGGG CGGCGGCGCG CGAAGAAGTC GGTGACGCGG CGCTGCTCCA
  421 TCTCGAGCGG CCGCCAGTGT GATGGATATC CCTTGTACAG CTCGTCCATG CCGCCGGTGG
  481 AGTGGCGGCC CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAGG
  541 TGATGTCCAA CTTGATGTTG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT
  601 TGTAGGTGGT CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT
  661 TGATCTCGCC CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCGGAGGAG GCCTCCCAGC
  721 CCATGGTCTT CTTCTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA
  781 CCTTGTAGAT GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC
  841 CGTCCTCGAA GTTCATCACG CGCTCCCACT TGAAGCCCTC GGGGAAAGGA CAGCTTCAAG
  901 TAGTCGGGGA TGTCGGCGGG GGTGCTTCAC GTAGGCCCTT GGAGCCCGTA CATGAACTGA
  961 GGGGACAGGA TGTCCCAAGG CGGAGGGCAG GGGGCCACCC TTTGGTTCAA CCTTTCAGCT
 1021 TTGACCGGAT CTGGGATGCC TTCGTTAGGA CGGATCCTCG ACCTCGCCTC GATCTCGAAC
1081 TCGATGAGCC
//
Primer: CMV-Forward RDB15441_A7Jvc.seq
>D05018A1_1_A7Jv_CMV-Forward_E03_05_ABI08.ab1
    1 CTGATCCCTT TCCGGCCCCC ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
  121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
  181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
  241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG
  301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG
  361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA
  421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA
  481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA
  541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC
  601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT
  661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG
  721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGGGC
  781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA
  841 AATATAAAGT AGTAAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA
  901 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA AATAGGAGCT TTTGTTCCTT GGGGTTCTTG
  961 GGGAGCAGCA GGAAGGCACT AATGGGGCGC AGCGTCAATG ACGGCCTGAC GGTACAGGCC
 1021 AGACAATTTA TGGTTCTGGG TATAGGTTGC AGCCAGCCAA GAACCAATTT GCTGGGAGAG
1081 GGACCTAATG
//
Primer: BGH_rev2 RDB15441_A7Jvd.seq
>D05018A1_1_A7Jv_BGH_rev_F10_16_ABI24.ab1
    1 GTGTCGGGGG TCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGGAAAGGGA
  901 CGTCAGCTTC CCCGACACAC CCACGGGAAT TGTCAGTGCC CAAACAGCCG AGCCCCTGTC
  961 CAGCAGCGGG CAGCAGGCGC GATGAGTTCG GCGGTGCATA GGGAGGGGGA AAGCGAAGTT
 1021 CCCGAAAGAA GCTGGACAGT GATGGCCATG GCCTCATCAA GTGGATGCGT CAGTACACAG
1081 TGCACACCCA CGTCTACGAT
//
Primer: SV40pro_F RDB15441_A7Jve.seq
>D05018A1_1_A7Jv_SV40pro_F_F03_06_ABI08.ab1
    1 CCTCGTTCCG TTGGGAATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
  781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG
  841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
  901 AACATACGAG CCCGGAAGCA TAAAGTGTAA AGCCTGGGGG TGCCTAATGA GTGAGCTAAC
  961 TCACATTAAA TTGCGTTGCG CTCACTGCCC CGCTTTCCAG TCGGGAAACC CTGGTCGTGC
 1021 CAGCCTGCAA TTAATGAATC GGGCCAACGC CGCGGGGAGA GCGGTTTGCC GTAATGGGGC
1081 GCTTCTTCCG GCCTTCCCTC GCCTTCACAC CTGTGGAACC TTTCTC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2017.11.15

GNP_filter3_RDBDEP_html_171106.pl