Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

(n1)StayGold/pRSET

Fluorescent protein StayGold for fusion to the C-terminus of target molecule

Catalog number RDB19605
Resource name (n1)StayGold/pRSET
Clone info. For fusion of StayGold to the C-terminus of a target molecule (X). n1 is the appendage that facilitates fusions to the N-terminus of StayGold. X-(n1)StayGold is required to terminate with the original C-terminus of StayGold. The gene for StayGold in this construct has been resynthesized with mammalian-preferred codons. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pRSET (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Ampicillin
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
 
Sequence (full) RDB19605zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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MTA, for use for not-for-profit academic purpose [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
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提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考 提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19605 (n1)StayGold/pRSET DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The (n1)StayGold/pRSET was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19605).

Reference section:

Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., Miyawaki, A., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19605_B2Eap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: 6xHis,T7 tag,Xpress tag,insert 5'
Sequence file: RDB19605_B2Eaa.seq check
>D08836A1_B2Ea_2_lacIpro-B_F_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCG CNNCGNNTCN GTGAGCGAGG AAGCGGAAGA GCGCCCAATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA
  121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT
  181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG
  241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC
  301 CGATGGTGTC TACAGGCGAG GAGCTGTTTA CCGGCGTGGT GCCCTTCAAG TTCCAGCTGA
  361 AGGGCACCAT CAACGGCAAG AGCTTCACCG TGGAAGGCGA GGGCGAGGGC AATAGCCACG
  421 AGGGCAGCCA CAAAGGCAAG TACGTGTGCA CCAGCGGCAA ACTGCCAATG TCTTGGGCCG
  481 CCCTGGGAAC TAGCTTCGGC TATGGCATGA AGTACTACAC CAAGTACCCC AGCGGCCTGA
  541 AGAACTGGTT CCACGAGGTG ATGCCCGAGG GCTTCACCTA CGACAGACAC ATCCAGTACA
  601 AGGGCGACGG CAGCATCCAC GCCAAGCACC AGCACTTCAT GAAGAACGGC ACCTACCACA
  661 ACATCGTGGA GTTCACCGGC CAGGACTTCA AGGAGAACAG CCCCGTGCTG ACCGGCGACA
  721 TGAACGTGAG CCTGCCCAAC GAGGTGCAGC ACATCCCCAG AGATGACGGC GTGGAGTGCC
  781 CAGTGACCCT GCTGTACCCT CTGCTGANCG ACAAGAGCAA GTGCGTGGAG GCCCACCANA
  841 ACACCATCTG CNAGCCCCTG CACAATCAGC CAGCCCCCGA TGTGCCATAC CNCNGGATCA
  901 GAAAGCANTA CACCCAGANC NAGGNNGANA CCGANGANAG AGANCACATC TGNNANANCG
  961 NNNNCNNNGA NGNCCNCCTG NNNNAATNNN AANCTNGNTN CNGNNTGNNN NCNAAGCCNN
1021 AAAGGAANNT GANNNNNNNN NN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,insert 3'
Sequence file: RDB19605_B2Eab.seq check
>D08836A1_B2Ea_2_f1ori_R3_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGC ACNGTTGGGA NGGCGANCGG TGCGGGCCTC TTCGCTATTA
   61 CGCCAGATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG
  121 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
  181 GGGCTTTGTT AGCAGCCGGA TCAAGCTTCG AATTCTTACA GGTGGGCCTC CAGGGTCTCG
  241 CTCTGGCAGA TGTGGTCTCT CTCCTCGGTG TCGTCCTTGC TCTGGGTGTA CTGCTTTCTG
  301 ATCCAGTGGT ATGGCACATC GGGGGCTGGC TGATTGTGCA GGGGCTTGCA GATGGTGTTC
  361 TGGTGGGCCT CCACGCACTT GCTCTTGTCG CTCAGCAGAG GGTACAGCAG GGTCACTGGG
  421 CACTCCACGC CGTCATCTCT GGGGATGTGC TGCACCTCGT TGGGCAGGCT CACGTTCATG
  481 TCGCCGGTCA GCACGGGGCT GTTCTCCTTG AAGTCCTGGC CGGTGAACTC CACGATGTTG
  541 TGGTAGGTGC CGTTCTTCAT GAAGTGCTGG TGCTTGGCGT GGATGCTGCC GTCGCCCTTG
  601 TACTGGATGT GTCTGTCGTA GGTGAAGCCC TCGGGCATCA CCTCGTGGAA CCAGTTCTTC
  661 AGGCCGCTGG GGTACTTGGT GTAGTACTTC ATGCCATAGC CGAAGCTAGT TCCCAGGGCG
  721 GCCCAAGACA TTGGCAGTTT GCCGCTGGTG CACACGTACT TGCCTTTGTG GCTGCCCTCG
  781 TGGCTATTGC CCTCGCCCTC GCCTTCCACG GTGAANCTCT TGCCGTTGAN GGTGCCCTTC
  841 ANCTGGAACT TGAANGGCAC CACGCCGGTA AACAGCTCCT CGCCTGTANA NACCATCGGA
  901 TCCTTATCGT CATCGTCNNA NAGATNCCGA NCCNTTTGCT GTCCNCCAGN CANGCTAGCC
  961 NTANCATGAT GANGANGANG ANGANAACCC NNNATATGTA TATNNNCNNN NNAAAGNNNA
1021 ANAANNNNNT TNNNNANGNN AANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]