Please review the QC test results indicated by icon below as well as clone information before placing your order.

(n1)StayGold/pRSET

Fluorescent protein StayGold for fusion to the C-terminus of target molecule

Sequence (full)	RDB19605zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19605_B2Eap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: 6xHis,T7 tag,Xpress tag,insert 5' Sequence file: RDB19605_B2Eaa.seq
>D08836A1_B2Ea_2_lacIpro-B_F_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCG CNNCGNNTCN GTGAGCGAGG AAGCGGAAGA GCGCCCAATA    61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA   121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT   181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG   241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC   301 CGATGGTGTC TACAGGCGAG GAGCTGTTTA CCGGCGTGGT GCCCTTCAAG TTCCAGCTGA   361 AGGGCACCAT CAACGGCAAG AGCTTCACCG TGGAAGGCGA GGGCGAGGGC AATAGCCACG   421 AGGGCAGCCA CAAAGGCAAG TACGTGTGCA CCAGCGGCAA ACTGCCAATG TCTTGGGCCG   481 CCCTGGGAAC TAGCTTCGGC TATGGCATGA AGTACTACAC CAAGTACCCC AGCGGCCTGA   541 AGAACTGGTT CCACGAGGTG ATGCCCGAGG GCTTCACCTA CGACAGACAC ATCCAGTACA   601 AGGGCGACGG CAGCATCCAC GCCAAGCACC AGCACTTCAT GAAGAACGGC ACCTACCACA   661 ACATCGTGGA GTTCACCGGC CAGGACTTCA AGGAGAACAG CCCCGTGCTG ACCGGCGACA   721 TGAACGTGAG CCTGCCCAAC GAGGTGCAGC ACATCCCCAG AGATGACGGC GTGGAGTGCC   781 CAGTGACCCT GCTGTACCCT CTGCTGANCG ACAAGAGCAA GTGCGTGGAG GCCCACCANA   841 ACACCATCTG CNAGCCCCTG CACAATCAGC CAGCCCCCGA TGTGCCATAC CNCNGGATCA   901 GAAAGCANTA CACCCAGANC NAGGNNGANA CCGANGANAG AGANCACATC TGNNANANCG   961 NNNNCNNNGA NGNCCNCCTG NNNNAATNNN AANCTNGNTN CNGNNTGNNN NCNAAGCCNN 1021 AAAGGAANNT GANNNNNNNN NN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter,insert 3' Sequence file: RDB19605_B2Eab.seq
>D08836A1_B2Ea_2_f1ori_R3_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGC ACNGTTGGGA NGGCGANCGG TGCGGGCCTC TTCGCTATTA    61 CGCCAGATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG   121 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC   181 GGGCTTTGTT AGCAGCCGGA TCAAGCTTCG AATTCTTACA GGTGGGCCTC CAGGGTCTCG   241 CTCTGGCAGA TGTGGTCTCT CTCCTCGGTG TCGTCCTTGC TCTGGGTGTA CTGCTTTCTG   301 ATCCAGTGGT ATGGCACATC GGGGGCTGGC TGATTGTGCA GGGGCTTGCA GATGGTGTTC   361 TGGTGGGCCT CCACGCACTT GCTCTTGTCG CTCAGCAGAG GGTACAGCAG GGTCACTGGG   421 CACTCCACGC CGTCATCTCT GGGGATGTGC TGCACCTCGT TGGGCAGGCT CACGTTCATG   481 TCGCCGGTCA GCACGGGGCT GTTCTCCTTG AAGTCCTGGC CGGTGAACTC CACGATGTTG   541 TGGTAGGTGC CGTTCTTCAT GAAGTGCTGG TGCTTGGCGT GGATGCTGCC GTCGCCCTTG   601 TACTGGATGT GTCTGTCGTA GGTGAAGCCC TCGGGCATCA CCTCGTGGAA CCAGTTCTTC   661 AGGCCGCTGG GGTACTTGGT GTAGTACTTC ATGCCATAGC CGAAGCTAGT TCCCAGGGCG   721 GCCCAAGACA TTGGCAGTTT GCCGCTGGTG CACACGTACT TGCCTTTGTG GCTGCCCTCG   781 TGGCTATTGC CCTCGCCCTC GCCTTCCACG GTGAANCTCT TGCCGTTGAN GGTGCCCTTC   841 ANCTGGAACT TGAANGGCAC CACGCCGGTA AACAGCTCCT CGCCTGTANA NACCATCGGA   901 TCCTTATCGT CATCGTCNNA NAGATNCCGA NCCNTTTGCT GTCCNCCAGN CANGCTAGCC   961 NTANCATGAT GANGANGANG ANGANAACCC NNNATATGTA TATNNNCNNN NNAAAGNNNA 1021 ANAANNNNNT TNNNNANGNN AANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl