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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

(n1)StayGold/pRSET

Fluorescent protein StayGold for fusion to the C-terminus of target molecule

Catalog number RDB19605
Resource name (n1)StayGold/pRSET
Clone info. For fusion of StayGold to the C-terminus of a target molecule (X). n1 is the appendage that facilitates fusions to the N-terminus of StayGold. X-(n1)StayGold is required to terminate with the original C-terminus of StayGold. The gene for StayGold in this construct has been resynthesized with mammalian-preferred codons. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pRSET (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Ampicillin
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank

External Database
Cytaeis uchidae GFP

            Reference sequence
              
             
            Sequence (full) RDB19605zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19605 (n1)StayGold/pRSET DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content 明るく、退色しにくい新規の緑色蛍光タンパク質 「StayGold」 (Japanese text)
            Featured content StayGold, highly photostable GFP (English text)
            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19605_B2Eap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: 6xHis,T7 tag,Xpress tag,insert 5'
            Sequence file: RDB19605_B2Eaa.seq check
            >D08836A1_B2Ea_2_lacIpro-B_F_B04_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNCG CNNCGNNTCN GTGAGCGAGG AAGCGGAAGA GCGCCCAATA
               61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA
              121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT
              181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG
              241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC
              301 CGATGGTGTC TACAGGCGAG GAGCTGTTTA CCGGCGTGGT GCCCTTCAAG TTCCAGCTGA
              361 AGGGCACCAT CAACGGCAAG AGCTTCACCG TGGAAGGCGA GGGCGAGGGC AATAGCCACG
              421 AGGGCAGCCA CAAAGGCAAG TACGTGTGCA CCAGCGGCAA ACTGCCAATG TCTTGGGCCG
              481 CCCTGGGAAC TAGCTTCGGC TATGGCATGA AGTACTACAC CAAGTACCCC AGCGGCCTGA
              541 AGAACTGGTT CCACGAGGTG ATGCCCGAGG GCTTCACCTA CGACAGACAC ATCCAGTACA
              601 AGGGCGACGG CAGCATCCAC GCCAAGCACC AGCACTTCAT GAAGAACGGC ACCTACCACA
              661 ACATCGTGGA GTTCACCGGC CAGGACTTCA AGGAGAACAG CCCCGTGCTG ACCGGCGACA
              721 TGAACGTGAG CCTGCCCAAC GAGGTGCAGC ACATCCCCAG AGATGACGGC GTGGAGTGCC
              781 CAGTGACCCT GCTGTACCCT CTGCTGANCG ACAAGAGCAA GTGCGTGGAG GCCCACCANA
              841 ACACCATCTG CNAGCCCCTG CACAATCAGC CAGCCCCCGA TGTGCCATAC CNCNGGATCA
              901 GAAAGCANTA CACCCAGANC NAGGNNGANA CCGANGANAG AGANCACATC TGNNANANCG
              961 NNNNCNNNGA NGNCCNCCTG NNNNAATNNN AANCTNGNTN CNGNNTGNNN NCNAAGCCNN
            1021 AAAGGAANNT GANNNNNNNN NN
            //
            Primer: f1ori_R3
            Region: T7 ter,insert 3'
            Sequence file: RDB19605_B2Eab.seq check
            >D08836A1_B2Ea_2_f1ori_R3_A08_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCGC ACNGTTGGGA NGGCGANCGG TGCGGGCCTC TTCGCTATTA
               61 CGCCAGATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG
              121 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
              181 GGGCTTTGTT AGCAGCCGGA TCAAGCTTCG AATTCTTACA GGTGGGCCTC CAGGGTCTCG
              241 CTCTGGCAGA TGTGGTCTCT CTCCTCGGTG TCGTCCTTGC TCTGGGTGTA CTGCTTTCTG
              301 ATCCAGTGGT ATGGCACATC GGGGGCTGGC TGATTGTGCA GGGGCTTGCA GATGGTGTTC
              361 TGGTGGGCCT CCACGCACTT GCTCTTGTCG CTCAGCAGAG GGTACAGCAG GGTCACTGGG
              421 CACTCCACGC CGTCATCTCT GGGGATGTGC TGCACCTCGT TGGGCAGGCT CACGTTCATG
              481 TCGCCGGTCA GCACGGGGCT GTTCTCCTTG AAGTCCTGGC CGGTGAACTC CACGATGTTG
              541 TGGTAGGTGC CGTTCTTCAT GAAGTGCTGG TGCTTGGCGT GGATGCTGCC GTCGCCCTTG
              601 TACTGGATGT GTCTGTCGTA GGTGAAGCCC TCGGGCATCA CCTCGTGGAA CCAGTTCTTC
              661 AGGCCGCTGG GGTACTTGGT GTAGTACTTC ATGCCATAGC CGAAGCTAGT TCCCAGGGCG
              721 GCCCAAGACA TTGGCAGTTT GCCGCTGGTG CACACGTACT TGCCTTTGTG GCTGCCCTCG
              781 TGGCTATTGC CCTCGCCCTC GCCTTCCACG GTGAANCTCT TGCCGTTGAN GGTGCCCTTC
              841 ANCTGGAACT TGAANGGCAC CACGCCGGTA AACAGCTCCT CGCCTGTANA NACCATCGGA
              901 TCCTTATCGT CATCGTCNNA NAGATNCCGA NCCNTTTGCT GTCCNCCAGN CANGCTAGCC
              961 NTANCATGAT GANGANGANG ANGANAACCC NNNATATGTA TATNNNCNNN NNAAAGNNNA
            1021 ANAANNNNNT TNNNNANGNN AANNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            2022.08.04

            GNP_filter3_RDBDEP_html_220727.pl