RDB15135 - YC3.60/pcDNA3

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YC3.60/pcDNA3 (#RDB15135)

Yellow cameleon calcium ion sensor clone YC3.60.

Clone info.	Calcium ion sensor clone YC3.60 Yellow cameleon having E104Q substitution in the 3rd Ca2+ binding site of CaM and the circular permutated cp173Venus. Mammalian expression plasmid.
Comment	Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178712.1. Nucleotide sequence of AB178712.1 will be updated.
Vector backbone	pcDNA3 (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	LB + Amp, 37oC
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	AB178712 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Sequence (full)	RDB15135hts01.seq Assembled from experimentally sequenced data.
Publication	Miyawaki, A., Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). [link to RRC of NBRP]
Publication	Nagai, T., Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). [link to RRC of NBRP]
Test sheet	RDB15135_B1Fgp1-2.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15135	YC3.60/pcDNA3	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The YC3.60/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15135).

Reference section:

Miyawaki, A., Nagai, T., Mizuno, H., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769. [link to RRC of NBRP]

Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M., Miyawaki, A., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769. [link to RRC of NBRP]
original	Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]
user_report	Omi, J., Phosphatidylserine synthesis controls oncogenic B cell receptor signaling in B cell lymphoma. J. Cell Biol. 223 (2): e202212074 (2024). PMID 38048228. [link to RRC of NBRP]