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YC3.60/pcDNA3

Yellow cameleon calcium ion sensor clone YC3.60.

Catalog number RDB15135
Resource name YC3.60/pcDNA3
Clone info. Calcium ion sensor clone YC3.60 Yellow cameleon having E104Q substitution in the 3rd Ca2+ binding site of CaM and the circular permutated cp173Venus. Mammalian expression plasmid.
Comment Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178712.1. Nucleotide sequence of AB178712.1 will be updated.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB178712 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15135 YC3.60/pcDNA3 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15135_A7H9p1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro,insert 5'
        Sequence file: RDB15135_A7H9a.seq check
        >D04498C2_A7H9_1_CMV-Forward_A05_02_ABI24.ab1
            1 ATCGGATCAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
           61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
          121 ATACGACTCA CTATAGGGAG ACCCAAGCTT GCGGCCGCCA CCATGGTGAG CAAGGGCGAG
          181 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
          241 AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
          301 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
          361 TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
          421 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT TCTTCAAGGA CGACGGCAAC
          481 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
          541 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
          601 ATCAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCCACTTC
          661 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
          721 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
          781 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
          841 GCCGCCCGCA TGCATGACCA ACTGACAGAA GAGCAGATTG CAGAGTTCAA AGAAGCCTTC
          901 TCATTATTCG ACAAGGGATG GGGACGGCAC CATCACCACA AAGGAACTTG GGCACCGTTA
          961 TGAGGTCGCT TGGGACAAAA CCCAACGGGA AGCAGAAATT GCAGGATATG ATCAAATGAA
         1021 GTCGATGCTG ATGGCAATGG GAACGATTTA CTTTCCTGAA TTTCTTACTA TGATGGGCTA
         1081 GAAAAAATGA AGGACACAGG ACAGCGAGAG AATCGAGAAG CATTCCGTGT TTTTGGAACA
        1141 AGGAATTGGG AACCGCGGTC CT
        //
        Primer: BGH_rev2
        Region: bGHpA,insert 3'
        Sequence file: RDB15135_A7H9b.seq check
        >D04498C2_A7H9_1_BGH_rev2_B05_05_ABI24.ab1
            1 CAATTCGGGG TTCAGGGAGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
           61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC
          121 TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTTACTCGAT
          181 GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA
          241 GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
          301 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
          361 GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC
          421 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA
          481 GCACTGCAGG CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC
          541 GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC
          601 GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC
          661 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGCCACCG CTGCCACCCT TGTACAGCTC
          721 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG
          781 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG
          841 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC
          901 GCCGTCCATG AGCTCCAGTG CCCCGGAGCT GGAGATCTTC TTGAACCGGT TGGCAGCGCT
          961 GACGGCAATG AAGTTTTTTC TTCCAGCGCC TCTTTCCCCC CCTTTTGCTG TCATCATTTG
         1021 TACAAACTCT TTCATAGTTT ACTTGGGCCC ATCACCCATC GATATCTGCT TTCCCTTATC
         1081 ATTTCATCAA CTTTCTTCAA TCTGTTAACT CTCCCGAGTT TGGTCATGAA CGTGACGTTA
        1141 ACTGGAAAGC AGCGGCCTTG ATGTGTTATA GC
        //
        Primer: SV40pro_F
        Region: SV40 pro_ori,NeoR
        Sequence file: RDB15135_A7H9c.seq check
        >15135_15135_A7H9_SV40pro_F_B02_02_ABI08.ab1
            1 CCCCCCTCCC TTTTTACCTT TTCAGAAAGT AGTGGAGGAG GCTTTTTTTG GAGGCCTAGG
           61 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG
          121 ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
          181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
          241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
          301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
          361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
          421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
          481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
          541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
          601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
          661 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
          721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC
          781 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA
          841 ATGGGCTGAC CGCTTCCTCG TGCTTTACGG GTATCGCCGC TCCCGATTCG CAGCGCATCG
          901 CCTTCTATCG CCTTCTTGAC GAGTTTCTTC TGAGCGGGAC TCTGGGGGTT TCGAAATGAC
          961 CGACCAAGCG ACGCCCAACC CTGCCCATCA CGAAGATTTC GATTCCACCC GCCCGCCCTT
         1021 TCTATGAAAA GGTTGGGGCT TTCGGAAATC GTTTTTTCCC GGGGACGCCC GGCCTGGGAT
         1081 GGATTCCTTC CAGCCGCGGG GAATCTCCAT GCCTTGGGAA GTTCTTTCGC CCAACCCCCC
        1141 AATCTTGTTT AAATTGCCAG CTTTAATGAT GGGTTTAACA AAATTAAAAA GGCC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Miyawaki, A., Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harb. Protoc. 2013 (11). pii: pdb.prot078642 (2013). PMID 24184769.
        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl