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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tdoxStayGold(c4)/pBS Coupler

Fluorescent protein tdoxStayGold for fusion to the N-terminus of target molecule

Catalog number RDB19612
Resource name tdoxStayGold(c4)/pBS Coupler
Clone info. For fusion of tdoxStayGold to the N-terminus of a target molecule (X). tdoxStayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, oxStayGold, c4, EV linker, n1, and oxStayGold. The gene for the former oxStayGold has been resynthesized with mammalian-preferred codons, and the gene for the latter oxStayGold has the jellyfish original codons. n1 and c4 are the appendages that facilitate fusions to the N- and C-termini of oxStayGold, respectively. tdoxStayGold(c4) encoded by this plasmid DNA is followed by a Coupler linker: (GGGGS)×3. oxStayGold is a variant of StayGold with two mutations: C174I and C208I; oxStayGold is well expressed also in the cytosolic fraction as StayGold. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pBS Coupler (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
 
Sequence (full) RDB19612zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Hirano, M. et al. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
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RDB19612 tdoxStayGold(c4)/pBS Coupler DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The tdoxStayGold(c4)/pBS Coupler was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19612).

Reference section:

Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., Miyawaki, A., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19612_B2Eap1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBAC_lac_F (Pr0544)
Region: T7 pro,insert 5'
Sequence file: RDB19612_B2Eaa.seq check
>D08836A8_B2Ea_1_pBAC_lac_F_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNC GCGNANACGA CTCNCTATAG GGCGAATTGG AGCTCCNCCG
   61 CGGTGGCGGC CGCAGCCACC ATGGTGTCTA CAGGCGAGGA GCTGTTTACC GGCGTGGTGC
  121 CCTTCAAGTT CCAGCTGAAG GGCACCATCA ACGGCAAGAG CTTCACCGTG GAAGGCGAGG
  181 GCGAGGGCAA TAGCCACGAG GGCAGCCACA AAGGCAAGTA CGTGTGCACC AGCGGCAAAC
  241 TGCCAATGTC TTGGGCCGCC CTGGGAACTA GCTTCGGCTA TGGCATGAAG TACTACACCA
  301 AGTACCCCAG CGGCCTGAAG AACTGGTTCC ACGAGGTGAT GCCCGAGGGC TTCACCTACG
  361 ACAGACACAT CCAGTACAAG GGCGACGGCA GCATCCACGC CAAGCACCAG CACTTCATGA
  421 AGAACGGCAC CTACCACAAC ATCGTGGAGT TCACCGGCCA GGACTTCAAG GAGAACAGCC
  481 CCGTGCTGAC CGGCGACATG AACGTGAGCC TGCCCAACGA GGTGCAGCAC ATCCCCAGAG
  541 ATGACGGCGT GGAGTGCCCA GTGACCCTGC TGTACCCTCT GCTGAGCGAC AAGAGCAAGT
  601 GCGTGGAGGC CTACCAGAAC ACCATCATCA AGCCCCTGCA CAATCAGCCA GCCCCCGATG
  661 TGCCATACCA CTGGATCAGA AAGCAGTACA CCCAGAGCAA GGACGACACC GAGGAGAGAG
  721 ANCACATCAT CCAGAGCGAG ACCCTGGANG CCCACCTGCC ATGGCACGAG CCTTCTGCTT
  781 CTGCCGTGGA ATTCAGTGCT GGTGGTTCTG CTGGANGAAG CGCCGGANGT TCCGCTGGNG
  841 GAAGCGCANG CGGCTCCNGC AGCGCTGGCG GGANTGCCGG GGGCAGTANC TCCGCANGAN
  901 GAAGTGCNNN GGAANCGCTG GTGGCAGTGC GGGGGGGTTC CGCNNNGGNA NCGGTTCCNC
  961 CNGNNNNNNT GNCNGNNGNA NCACNTCTGN NGGGGGGNNN NGNNNNNNGN NNNNNNNGNN
1021 NGNNNNNNNN NN
//
Primer: reverse-2 (Pr0100)
Region: T3 pro,(GGGGS)x3,insert 3'
Sequence file: RDB19612_B2Eab.seq check
>D08836A8_B2Ea_1_reverse-2_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNGCNAGCGC GCNNTTAACC CTCACTAAAG GGAACAAAAG CTGGGTACCG
   61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATCG ATCCGCCACC GCCAGAGCCA
  121 CCTCCGCCTG AACCGCCTCC ACCATCGAAT TCCTGCAGCC CGGGGGATCC TACTGCCGAT
  181 GCTGAAGGCT CATGCCAAGG TAAGTGTGCT TCGAGAGTCT CTGATTGAAT AATATGATCA
  241 CGTTCCTCGG TATCATCTTT GCTTTGTGTG TATTGTTTAC GAATCCAGTG ATATGGGACA
  301 TCAGGTGCTG GTTGATTATG AAGAGGCTTG ATGATTGTAT TTTGGTAAGC CTCAACGCAT
  361 TTTGATTTAT CCGATAATAA AGGATAAAGC AAGGTCACTG GGCATTCTAC TCCATCATCT
  421 CTGGGTATAT GTTGAACTTC ATTCGGTAAT GAGACATTCA TATCTCCAGT TAAGACTGGA
  481 CTATTTTCTT TAAAATCCTG ACCAGTAAAT TCAACAATGT TGTGATAAGT CCCATTTTTC
  541 ATAAAGTGTT GGTGTTTTGC ATGGATACTC CCATCGCCTT TATATTGAAT ATGACGATCG
  601 TAGGTAAAGC CTTCAGGCAT TACTTCATGA AACCAGTTCT TCAGTCCACT AGGATATTTG
  661 GTATAATATT TCATTCCATA ACCAAAGGAT GTTCCAAGTG CTGCCCATGA CATCGGTAGT
  721 TTTCCACTTG TACAAACATA TTTTCCTTTA TGAGAACCTT CATGTGAATT TCCTTCACCT
  781 TCGCCTTCAA CGGTAAACGA TTTGCCATTG ATGGTTCCTT TAAGTTGNAN TTTAAATGGC
  841 ACCACCCCGG TGAACAGCTC CNNNNNGNAC TCACCATAAG CTTCCCACCG GCGCTANCGC
  901 CGGCNCTCCN TCCGGCGGAA CCGCCAGCAN ANCCNCCTGC ANANGTGGAN CCTNCAGCGC
  961 TACCNCCTGC NCTTCCNNAN CCCCCNNGNA NANCCNNNNG NNCTCCNNCC ANCNGNANCN
1021 NNNNGNNNNA CCCCNNNNAN ANNNGNNTNN NNNNNGNNNN NNNNNN
//
Primer: hStayGold_F1 (Pr1213)
Region: insert mid
Sequence file: RDB19612_B2Eac.seq check
>D08836A8_B2Ea_1_hStayGold_F1_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNCCTGCCNN NCGAGGTGCA GCACATCCCC NGAGATGACG
   61 GCGTGGAGTG CCCAGTGACC CTGCTGTACC CTCTGCTGAG CGACAAGAGC AAGTGCGTGG
  121 AGGCCTACCA GAACACCATC ATCAAGCCCC TGCACAATCA GCCAGCCCCC GATGTGCCAT
  181 ACCACTGGAT CAGAAAGCAG TACACCCAGA GCAAGGACGA CACCGAGGAG AGAGACCACA
  241 TCATCCAGAG CGAGACCCTG GAGGCCCACC TGCCATGGCA CGAGCCTTCT GCTTCTGCCG
  301 TGGAATTCAG TGCTGGTGGT TCTGCTGGAG GAAGCGCCGG AGGTTCCGCT GGGGGAAGCG
  361 CAGGCGGCTC CGGCAGCGCT GGCGGGAGTG CCGGGGGCAG TACCTCCGCA GGAGGAAGTG
  421 CAGGGGGAAG CGCTGGTGGC AGTGCGGGGG GTTCCGCAGG CGGTAGCGGT TCCGCCGGTG
  481 GATCTGCCGG TGGAAGCACA TCTGCTGGGG GTAGTGCAGG AGGGTCCGCT GGTGGGAGCG
  541 CAGGTGGATC TGCCGGGGGG TCTGGAAGTG CAGGTGGTAG CGCTGGAGGG TCCACATCTG
  601 CAGGTGGGTC TGCTGGCGGT TCCGCCGGAG GGAGTGCCGG CGGTAGCGCC GGTGGGAAGC
  661 TTATGGTGAG TACAGGGGAG GAGCTGTTCA CCGGGGTGGT GCCATTTAAA TTTCAACTTA
  721 AAGGAACCAT CAATGGCAAA TCGTTTACCG TTGAANGCGA ANGTGAAGGA AATTCACATG
  781 AANGTTCTCA TAAAGGAAAA TATGTTTGTA CAAGTGGAAA ACTACCGATG TCATGGGCAG
  841 CACTTGGAAC ATCCTTTGGT TATGGAATGA AATATTATAC CNAATATCCT ANTGNANTGA
  901 AGAANTGGTT TCATGAANTA ATGNCTGAAN GNTTTANNTA CNATCGTCNT ATTCNATATA
  961 AANGCNANGG NNGTATNNNT GCNAAACACN NACNNTTNNT GAAAAATGGN NNNTNTCNNN
1021 NNNTNNNTNN NNNTNNNNNG NNNNNNNNTT NNANNNNNNN NNN
//
Primer: EV_linker_F1 (Pr1214)
Region: insert mid
Sequence file: RDB19612_B2Ead.seq check
>D08836A8_B2Ea_1_EV_linker_F1_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NGGNNCGCNN GTGGNNCTGC CGGGGGGTCT GGAAGTGCAG
   61 GTGGTAGCGC TGGAGGGTCC ACATCTGCAG GTGGGTCTGC TGGCGGTTCC GCCGGAGGGA
  121 GTGCCGGCGG TAGCGCCGGT GGGAAGCTTA TGGTGAGTAC AGGGGAGGAG CTGTTCACCG
  181 GGGTGGTGCC ATTTAAATTT CAACTTAAAG GAACCATCAA TGGCAAATCG TTTACCGTTG
  241 AAGGCGAAGG TGAAGGAAAT TCACATGAAG GTTCTCATAA AGGAAAATAT GTTTGTACAA
  301 GTGGAAAACT ACCGATGTCA TGGGCAGCAC TTGGAACATC CTTTGGTTAT GGAATGAAAT
  361 ATTATACCAA ATATCCTAGT GGACTGAAGA ACTGGTTTCA TGAAGTAATG CCTGAAGGCT
  421 TTACCTACGA TCGTCATATT CAATATAAAG GCGATGGGAG TATCCATGCA AAACACCAAC
  481 ACTTTATGAA AAATGGGACT TATCACAACA TTGTTGAATT TACTGGTCAG GATTTTAAAG
  541 AAAATAGTCC AGTCTTAACT GGAGATATGA ATGTCTCATT ACCGAATGAA GTTCAACATA
  601 TACCCAGAGA TGATGGAGTA GAATGCCCAG TGACCTTGCT TTATCCTTTA TTATCGGATA
  661 AATCAAAATG CGTTGAGGCT TACCAAAATA CAATCATCAA GCCTCTTCAT AATCAACCAG
  721 CACCTGATGT CCCATATCAC TGGATTCGTA AACAATACAC ACAAAGCAAA GATGANACCG
  781 AGGAACGTGA TCATATTATT CAATCAGAGA CTCTCGAAGC ACACTTACCT TGGCATGANC
  841 CTTCAGCATC GGCAGTANGA TCCNNNGGGC TGCANGAATT CNATGGTGGA NGCGGNTCNN
  901 NCGGANGNGG CTCTGGCGGN GGCGGNNCNN TCAAGCTTAT CGATANCGTC NANCNCNANG
  961 GGGGGCCCGG NACCCANCTT TTGNTCCNNT TNNTGANGGN TNNNNTGNNN NCTTNNNNNN
1021 NNCNNNGNNC NNANCNNNTN NNNNNNNNN
//
Primer: pBR322-ori-R (Pr1218)
Region: pBR322 ori 3',AmpR
Sequence file: RDB19612_B2Eae.seq check
>D08836A8_B2Ea_1-pBR322-ori-R_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGNA GCTCTTGNNC CGGCAAACNA ACCACCGCTG GTAGCGGTGG
   61 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
  121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
  181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
  241 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
  301 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
  361 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
  421 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
  481 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
  541 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
  601 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
  661 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
  721 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
  781 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGANTGGTG AGTACTCAAC
  841 CAAGTCATTC TGANAATAGT GTATGCGGCG ANCGANTTGC TCTTGNCNNG CGTCAATACN
  901 GGATAATACC GCGCCNCATA NCANAANTTT NAAANNGCTC ATCATTGGAA AANGNTCTTC
  961 GGGGCGAAAA NNCTCNNNNN NNNNANNNCT NNNGNNNTNC NNTTCGATGT AACCCNNNNN
1021 NNNNN
//
Primer: f1ori_F (Pr0280)
Region: f1 ori,AmpR pro,AmpR
Sequence file: RDB19612_B2Eaf.seq check
>D08836A8_B2Ea_1-f1ori_F_C05_08_ABI24.ab1
    1 NNNNNNNNNN NGNGNNNGNA GNGNAGCGGT CNNGCTGCGC GTAACCNCCA CACCCGCCGC
   61 GCTTAATGCG CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
  121 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
  181 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
  241 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
  301 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
  361 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
  421 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
  481 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
  541 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
  601 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
  661 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
  721 TGAAGCCATA CCAAACGACG AGCGTGNCAC CACGATGCCT GTAGCAATGG CAACAACGTT
  781 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACNAT TAATAGACTG
  841 GATGGAGGCG GATAAAGTTG CANGACCACT TCTGCGCTCG GNCCTTNCNG CTGGNTGGNT
  901 TANTGNTGAT AAATCTGGAN CCNGNNANCG TGGNNCTCGC GNNATCATTG CAGCACTNGG
  961 GNNNGANNGT AAGCCNNNCC GTATCGNANT NNTCNACNCN NNNGGNNNNN NNNNANNTAN
1021 GGNNGNNNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]