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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tdoxStayGold(c4)/pBS Coupler

Fluorescent protein tdoxStayGold for fusion to the N-terminus of target molecule

Catalog number RDB19612
Resource name tdoxStayGold(c4)/pBS Coupler
Clone info. For fusion of tdoxStayGold to the N-terminus of a target molecule (X). tdoxStayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, oxStayGold, c4, EV linker, n1, and oxStayGold. The gene for the former oxStayGold has been resynthesized with mammalian-preferred codons, and the gene for the latter oxStayGold has the jellyfish original codons. n1 and c4 are the appendages that facilitate fusions to the N- and C-termini of oxStayGold, respectively. tdoxStayGold(c4) encoded by this plasmid DNA is followed by a Coupler linker: (GGGGS)×3. oxStayGold is a variant of StayGold with two mutations: C174I and C208I; oxStayGold is well expressed also in the cytosolic fraction as StayGold. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pBS Coupler (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank

External Database
Cytaeis uchidae GFP

            Reference sequence
              
             
            Sequence (full) RDB19612zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19612 tdoxStayGold(c4)/pBS Coupler DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content 明るく、退色しにくい新規の緑色蛍光タンパク質 「StayGold」 (Japanese text)
            Featured content StayGold, highly photostable GFP (English text)
            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19612_B2Eap1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pBAC_lac_F
            Region: T7 pro,insert 5'
            Sequence file: RDB19612_B2Eaa.seq check
            >D08836A8_B2Ea_1_pBAC_lac_F_D08_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCNC GCGNANACGA CTCNCTATAG GGCGAATTGG AGCTCCNCCG
               61 CGGTGGCGGC CGCAGCCACC ATGGTGTCTA CAGGCGAGGA GCTGTTTACC GGCGTGGTGC
              121 CCTTCAAGTT CCAGCTGAAG GGCACCATCA ACGGCAAGAG CTTCACCGTG GAAGGCGAGG
              181 GCGAGGGCAA TAGCCACGAG GGCAGCCACA AAGGCAAGTA CGTGTGCACC AGCGGCAAAC
              241 TGCCAATGTC TTGGGCCGCC CTGGGAACTA GCTTCGGCTA TGGCATGAAG TACTACACCA
              301 AGTACCCCAG CGGCCTGAAG AACTGGTTCC ACGAGGTGAT GCCCGAGGGC TTCACCTACG
              361 ACAGACACAT CCAGTACAAG GGCGACGGCA GCATCCACGC CAAGCACCAG CACTTCATGA
              421 AGAACGGCAC CTACCACAAC ATCGTGGAGT TCACCGGCCA GGACTTCAAG GAGAACAGCC
              481 CCGTGCTGAC CGGCGACATG AACGTGAGCC TGCCCAACGA GGTGCAGCAC ATCCCCAGAG
              541 ATGACGGCGT GGAGTGCCCA GTGACCCTGC TGTACCCTCT GCTGAGCGAC AAGAGCAAGT
              601 GCGTGGAGGC CTACCAGAAC ACCATCATCA AGCCCCTGCA CAATCAGCCA GCCCCCGATG
              661 TGCCATACCA CTGGATCAGA AAGCAGTACA CCCAGAGCAA GGACGACACC GAGGAGAGAG
              721 ANCACATCAT CCAGAGCGAG ACCCTGGANG CCCACCTGCC ATGGCACGAG CCTTCTGCTT
              781 CTGCCGTGGA ATTCAGTGCT GGTGGTTCTG CTGGANGAAG CGCCGGANGT TCCGCTGGNG
              841 GAAGCGCANG CGGCTCCNGC AGCGCTGGCG GGANTGCCGG GGGCAGTANC TCCGCANGAN
              901 GAAGTGCNNN GGAANCGCTG GTGGCAGTGC GGGGGGGTTC CGCNNNGGNA NCGGTTCCNC
              961 CNGNNNNNNT GNCNGNNGNA NCACNTCTGN NGGGGGGNNN NGNNNNNNGN NNNNNNNGNN
            1021 NGNNNNNNNN NN
            //
            Primer: reverse-2
            Region: T3 pro,(GGGGS)x3,insert 3'
            Sequence file: RDB19612_B2Eab.seq check
            >D08836A8_B2Ea_1_reverse-2_E08_14_ABI24.ab1
                1 NNNNNNNNNN NNGCNAGCGC GCNNTTAACC CTCACTAAAG GGAACAAAAG CTGGGTACCG
               61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATCG ATCCGCCACC GCCAGAGCCA
              121 CCTCCGCCTG AACCGCCTCC ACCATCGAAT TCCTGCAGCC CGGGGGATCC TACTGCCGAT
              181 GCTGAAGGCT CATGCCAAGG TAAGTGTGCT TCGAGAGTCT CTGATTGAAT AATATGATCA
              241 CGTTCCTCGG TATCATCTTT GCTTTGTGTG TATTGTTTAC GAATCCAGTG ATATGGGACA
              301 TCAGGTGCTG GTTGATTATG AAGAGGCTTG ATGATTGTAT TTTGGTAAGC CTCAACGCAT
              361 TTTGATTTAT CCGATAATAA AGGATAAAGC AAGGTCACTG GGCATTCTAC TCCATCATCT
              421 CTGGGTATAT GTTGAACTTC ATTCGGTAAT GAGACATTCA TATCTCCAGT TAAGACTGGA
              481 CTATTTTCTT TAAAATCCTG ACCAGTAAAT TCAACAATGT TGTGATAAGT CCCATTTTTC
              541 ATAAAGTGTT GGTGTTTTGC ATGGATACTC CCATCGCCTT TATATTGAAT ATGACGATCG
              601 TAGGTAAAGC CTTCAGGCAT TACTTCATGA AACCAGTTCT TCAGTCCACT AGGATATTTG
              661 GTATAATATT TCATTCCATA ACCAAAGGAT GTTCCAAGTG CTGCCCATGA CATCGGTAGT
              721 TTTCCACTTG TACAAACATA TTTTCCTTTA TGAGAACCTT CATGTGAATT TCCTTCACCT
              781 TCGCCTTCAA CGGTAAACGA TTTGCCATTG ATGGTTCCTT TAAGTTGNAN TTTAAATGGC
              841 ACCACCCCGG TGAACAGCTC CNNNNNGNAC TCACCATAAG CTTCCCACCG GCGCTANCGC
              901 CGGCNCTCCN TCCGGCGGAA CCGCCAGCAN ANCCNCCTGC ANANGTGGAN CCTNCAGCGC
              961 TACCNCCTGC NCTTCCNNAN CCCCCNNGNA NANCCNNNNG NNCTCCNNCC ANCNGNANCN
            1021 NNNNGNNNNA CCCCNNNNAN ANNNGNNTNN NNNNNGNNNN NNNNNN
            //
            Primer: hStayGold_F1
            Region: insert mid
            Sequence file: RDB19612_B2Eac.seq check
            >D08836A8_B2Ea_1_hStayGold_F1_F08_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNGNN NNCCTGCCNN NCGAGGTGCA GCACATCCCC NGAGATGACG
               61 GCGTGGAGTG CCCAGTGACC CTGCTGTACC CTCTGCTGAG CGACAAGAGC AAGTGCGTGG
              121 AGGCCTACCA GAACACCATC ATCAAGCCCC TGCACAATCA GCCAGCCCCC GATGTGCCAT
              181 ACCACTGGAT CAGAAAGCAG TACACCCAGA GCAAGGACGA CACCGAGGAG AGAGACCACA
              241 TCATCCAGAG CGAGACCCTG GAGGCCCACC TGCCATGGCA CGAGCCTTCT GCTTCTGCCG
              301 TGGAATTCAG TGCTGGTGGT TCTGCTGGAG GAAGCGCCGG AGGTTCCGCT GGGGGAAGCG
              361 CAGGCGGCTC CGGCAGCGCT GGCGGGAGTG CCGGGGGCAG TACCTCCGCA GGAGGAAGTG
              421 CAGGGGGAAG CGCTGGTGGC AGTGCGGGGG GTTCCGCAGG CGGTAGCGGT TCCGCCGGTG
              481 GATCTGCCGG TGGAAGCACA TCTGCTGGGG GTAGTGCAGG AGGGTCCGCT GGTGGGAGCG
              541 CAGGTGGATC TGCCGGGGGG TCTGGAAGTG CAGGTGGTAG CGCTGGAGGG TCCACATCTG
              601 CAGGTGGGTC TGCTGGCGGT TCCGCCGGAG GGAGTGCCGG CGGTAGCGCC GGTGGGAAGC
              661 TTATGGTGAG TACAGGGGAG GAGCTGTTCA CCGGGGTGGT GCCATTTAAA TTTCAACTTA
              721 AAGGAACCAT CAATGGCAAA TCGTTTACCG TTGAANGCGA ANGTGAAGGA AATTCACATG
              781 AANGTTCTCA TAAAGGAAAA TATGTTTGTA CAAGTGGAAA ACTACCGATG TCATGGGCAG
              841 CACTTGGAAC ATCCTTTGGT TATGGAATGA AATATTATAC CNAATATCCT ANTGNANTGA
              901 AGAANTGGTT TCATGAANTA ATGNCTGAAN GNTTTANNTA CNATCGTCNT ATTCNATATA
              961 AANGCNANGG NNGTATNNNT GCNAAACACN NACNNTTNNT GAAAAATGGN NNNTNTCNNN
            1021 NNNTNNNTNN NNNTNNNNNG NNNNNNNNTT NNANNNNNNN NNN
            //
            Primer: EV_linker_F1
            Region: insert mid
            Sequence file: RDB19612_B2Ead.seq check
            >D08836A8_B2Ea_1_EV_linker_F1_G08_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGN NGGNNCGCNN GTGGNNCTGC CGGGGGGTCT GGAAGTGCAG
               61 GTGGTAGCGC TGGAGGGTCC ACATCTGCAG GTGGGTCTGC TGGCGGTTCC GCCGGAGGGA
              121 GTGCCGGCGG TAGCGCCGGT GGGAAGCTTA TGGTGAGTAC AGGGGAGGAG CTGTTCACCG
              181 GGGTGGTGCC ATTTAAATTT CAACTTAAAG GAACCATCAA TGGCAAATCG TTTACCGTTG
              241 AAGGCGAAGG TGAAGGAAAT TCACATGAAG GTTCTCATAA AGGAAAATAT GTTTGTACAA
              301 GTGGAAAACT ACCGATGTCA TGGGCAGCAC TTGGAACATC CTTTGGTTAT GGAATGAAAT
              361 ATTATACCAA ATATCCTAGT GGACTGAAGA ACTGGTTTCA TGAAGTAATG CCTGAAGGCT
              421 TTACCTACGA TCGTCATATT CAATATAAAG GCGATGGGAG TATCCATGCA AAACACCAAC
              481 ACTTTATGAA AAATGGGACT TATCACAACA TTGTTGAATT TACTGGTCAG GATTTTAAAG
              541 AAAATAGTCC AGTCTTAACT GGAGATATGA ATGTCTCATT ACCGAATGAA GTTCAACATA
              601 TACCCAGAGA TGATGGAGTA GAATGCCCAG TGACCTTGCT TTATCCTTTA TTATCGGATA
              661 AATCAAAATG CGTTGAGGCT TACCAAAATA CAATCATCAA GCCTCTTCAT AATCAACCAG
              721 CACCTGATGT CCCATATCAC TGGATTCGTA AACAATACAC ACAAAGCAAA GATGANACCG
              781 AGGAACGTGA TCATATTATT CAATCAGAGA CTCTCGAAGC ACACTTACCT TGGCATGANC
              841 CTTCAGCATC GGCAGTANGA TCCNNNGGGC TGCANGAATT CNATGGTGGA NGCGGNTCNN
              901 NCGGANGNGG CTCTGGCGGN GGCGGNNCNN TCAAGCTTAT CGATANCGTC NANCNCNANG
              961 GGGGGCCCGG NACCCANCTT TTGNTCCNNT TNNTGANGGN TNNNNTGNNN NCTTNNNNNN
            1021 NNCNNNGNNC NNANCNNNTN NNNNNNNNN
            //
            Primer: pBR322-ori-R
            Region: pBR322 ori 3',AmpR
            Sequence file: RDB19612_B2Eae.seq check
            >D08836A8_B2Ea_1-pBR322-ori-R_C04_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGGNA GCTCTTGNNC CGGCAAACNA ACCACCGCTG GTAGCGGTGG
               61 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
              121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
              181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
              241 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
              301 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
              361 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
              421 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
              481 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
              541 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
              601 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
              661 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
              721 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
              781 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGANTGGTG AGTACTCAAC
              841 CAAGTCATTC TGANAATAGT GTATGCGGCG ANCGANTTGC TCTTGNCNNG CGTCAATACN
              901 GGATAATACC GCGCCNCATA NCANAANTTT NAAANNGCTC ATCATTGGAA AANGNTCTTC
              961 GGGGCGAAAA NNCTCNNNNN NNNNANNNCT NNNGNNNTNC NNTTCGATGT AACCCNNNNN
            1021 NNNNN
            //
            Primer: f1ori_F
            Region: f1 ori,AmpR pro,AmpR
            Sequence file: RDB19612_B2Eaf.seq check
            >D08836A8_B2Ea_1-f1ori_F_C05_08_ABI24.ab1
                1 NNNNNNNNNN NGNGNNNGNA GNGNAGCGGT CNNGCTGCGC GTAACCNCCA CACCCGCCGC
               61 GCTTAATGCG CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
              121 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
              181 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
              241 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
              301 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
              361 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
              421 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
              481 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
              541 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
              601 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
              661 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
              721 TGAAGCCATA CCAAACGACG AGCGTGNCAC CACGATGCCT GTAGCAATGG CAACAACGTT
              781 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACNAT TAATAGACTG
              841 GATGGAGGCG GATAAAGTTG CANGACCACT TCTGCGCTCG GNCCTTNCNG CTGGNTGGNT
              901 TANTGNTGAT AAATCTGGAN CCNGNNANCG TGGNNCTCGC GNNATCATTG CAGCACTNGG
              961 GNNNGANNGT AAGCCNNNCC GTATCGNANT NNTCNACNCN NNNGGNNNNN NNNNANNTAN
            1021 GGNNGNNNNA NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl