Please review the QC test results indicated by icon below as well as clone information before placing your order.

tdoxStayGold(c4)/pBS Coupler

Fluorescent protein tdoxStayGold for fusion to the N-terminus of target molecule

Sequence (full)	RDB19612zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19612_B2Eap1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBAC_lac_F (Pr0544) Region: T7 pro,insert 5' Sequence file: RDB19612_B2Eaa.seq
>D08836A8_B2Ea_1_pBAC_lac_F_D08_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNC GCGNANACGA CTCNCTATAG GGCGAATTGG AGCTCCNCCG    61 CGGTGGCGGC CGCAGCCACC ATGGTGTCTA CAGGCGAGGA GCTGTTTACC GGCGTGGTGC   121 CCTTCAAGTT CCAGCTGAAG GGCACCATCA ACGGCAAGAG CTTCACCGTG GAAGGCGAGG   181 GCGAGGGCAA TAGCCACGAG GGCAGCCACA AAGGCAAGTA CGTGTGCACC AGCGGCAAAC   241 TGCCAATGTC TTGGGCCGCC CTGGGAACTA GCTTCGGCTA TGGCATGAAG TACTACACCA   301 AGTACCCCAG CGGCCTGAAG AACTGGTTCC ACGAGGTGAT GCCCGAGGGC TTCACCTACG   361 ACAGACACAT CCAGTACAAG GGCGACGGCA GCATCCACGC CAAGCACCAG CACTTCATGA   421 AGAACGGCAC CTACCACAAC ATCGTGGAGT TCACCGGCCA GGACTTCAAG GAGAACAGCC   481 CCGTGCTGAC CGGCGACATG AACGTGAGCC TGCCCAACGA GGTGCAGCAC ATCCCCAGAG   541 ATGACGGCGT GGAGTGCCCA GTGACCCTGC TGTACCCTCT GCTGAGCGAC AAGAGCAAGT   601 GCGTGGAGGC CTACCAGAAC ACCATCATCA AGCCCCTGCA CAATCAGCCA GCCCCCGATG   661 TGCCATACCA CTGGATCAGA AAGCAGTACA CCCAGAGCAA GGACGACACC GAGGAGAGAG   721 ANCACATCAT CCAGAGCGAG ACCCTGGANG CCCACCTGCC ATGGCACGAG CCTTCTGCTT   781 CTGCCGTGGA ATTCAGTGCT GGTGGTTCTG CTGGANGAAG CGCCGGANGT TCCGCTGGNG   841 GAAGCGCANG CGGCTCCNGC AGCGCTGGCG GGANTGCCGG GGGCAGTANC TCCGCANGAN   901 GAAGTGCNNN GGAANCGCTG GTGGCAGTGC GGGGGGGTTC CGCNNNGGNA NCGGTTCCNC   961 CNGNNNNNNT GNCNGNNGNA NCACNTCTGN NGGGGGGNNN NGNNNNNNGN NNNNNNNGNN 1021 NGNNNNNNNN NN //
Primer: reverse-2 (Pr0100) Region: T3 pro,(GGGGS)x3,insert 3' Sequence file: RDB19612_B2Eab.seq
>D08836A8_B2Ea_1_reverse-2_E08_14_ABI24.ab1     1 NNNNNNNNNN NNGCNAGCGC GCNNTTAACC CTCACTAAAG GGAACAAAAG CTGGGTACCG    61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATCG ATCCGCCACC GCCAGAGCCA   121 CCTCCGCCTG AACCGCCTCC ACCATCGAAT TCCTGCAGCC CGGGGGATCC TACTGCCGAT   181 GCTGAAGGCT CATGCCAAGG TAAGTGTGCT TCGAGAGTCT CTGATTGAAT AATATGATCA   241 CGTTCCTCGG TATCATCTTT GCTTTGTGTG TATTGTTTAC GAATCCAGTG ATATGGGACA   301 TCAGGTGCTG GTTGATTATG AAGAGGCTTG ATGATTGTAT TTTGGTAAGC CTCAACGCAT   361 TTTGATTTAT CCGATAATAA AGGATAAAGC AAGGTCACTG GGCATTCTAC TCCATCATCT   421 CTGGGTATAT GTTGAACTTC ATTCGGTAAT GAGACATTCA TATCTCCAGT TAAGACTGGA   481 CTATTTTCTT TAAAATCCTG ACCAGTAAAT TCAACAATGT TGTGATAAGT CCCATTTTTC   541 ATAAAGTGTT GGTGTTTTGC ATGGATACTC CCATCGCCTT TATATTGAAT ATGACGATCG   601 TAGGTAAAGC CTTCAGGCAT TACTTCATGA AACCAGTTCT TCAGTCCACT AGGATATTTG   661 GTATAATATT TCATTCCATA ACCAAAGGAT GTTCCAAGTG CTGCCCATGA CATCGGTAGT   721 TTTCCACTTG TACAAACATA TTTTCCTTTA TGAGAACCTT CATGTGAATT TCCTTCACCT   781 TCGCCTTCAA CGGTAAACGA TTTGCCATTG ATGGTTCCTT TAAGTTGNAN TTTAAATGGC   841 ACCACCCCGG TGAACAGCTC CNNNNNGNAC TCACCATAAG CTTCCCACCG GCGCTANCGC   901 CGGCNCTCCN TCCGGCGGAA CCGCCAGCAN ANCCNCCTGC ANANGTGGAN CCTNCAGCGC   961 TACCNCCTGC NCTTCCNNAN CCCCCNNGNA NANCCNNNNG NNCTCCNNCC ANCNGNANCN 1021 NNNNGNNNNA CCCCNNNNAN ANNNGNNTNN NNNNNGNNNN NNNNNN //
Primer: hStayGold_F1 (Pr1213) Region: insert mid Sequence file: RDB19612_B2Eac.seq
>D08836A8_B2Ea_1_hStayGold_F1_F08_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN NNCCTGCCNN NCGAGGTGCA GCACATCCCC NGAGATGACG    61 GCGTGGAGTG CCCAGTGACC CTGCTGTACC CTCTGCTGAG CGACAAGAGC AAGTGCGTGG   121 AGGCCTACCA GAACACCATC ATCAAGCCCC TGCACAATCA GCCAGCCCCC GATGTGCCAT   181 ACCACTGGAT CAGAAAGCAG TACACCCAGA GCAAGGACGA CACCGAGGAG AGAGACCACA   241 TCATCCAGAG CGAGACCCTG GAGGCCCACC TGCCATGGCA CGAGCCTTCT GCTTCTGCCG   301 TGGAATTCAG TGCTGGTGGT TCTGCTGGAG GAAGCGCCGG AGGTTCCGCT GGGGGAAGCG   361 CAGGCGGCTC CGGCAGCGCT GGCGGGAGTG CCGGGGGCAG TACCTCCGCA GGAGGAAGTG   421 CAGGGGGAAG CGCTGGTGGC AGTGCGGGGG GTTCCGCAGG CGGTAGCGGT TCCGCCGGTG   481 GATCTGCCGG TGGAAGCACA TCTGCTGGGG GTAGTGCAGG AGGGTCCGCT GGTGGGAGCG   541 CAGGTGGATC TGCCGGGGGG TCTGGAAGTG CAGGTGGTAG CGCTGGAGGG TCCACATCTG   601 CAGGTGGGTC TGCTGGCGGT TCCGCCGGAG GGAGTGCCGG CGGTAGCGCC GGTGGGAAGC   661 TTATGGTGAG TACAGGGGAG GAGCTGTTCA CCGGGGTGGT GCCATTTAAA TTTCAACTTA   721 AAGGAACCAT CAATGGCAAA TCGTTTACCG TTGAANGCGA ANGTGAAGGA AATTCACATG   781 AANGTTCTCA TAAAGGAAAA TATGTTTGTA CAAGTGGAAA ACTACCGATG TCATGGGCAG   841 CACTTGGAAC ATCCTTTGGT TATGGAATGA AATATTATAC CNAATATCCT ANTGNANTGA   901 AGAANTGGTT TCATGAANTA ATGNCTGAAN GNTTTANNTA CNATCGTCNT ATTCNATATA   961 AANGCNANGG NNGTATNNNT GCNAAACACN NACNNTTNNT GAAAAATGGN NNNTNTCNNN 1021 NNNTNNNTNN NNNTNNNNNG NNNNNNNNTT NNANNNNNNN NNN //
Primer: EV_linker_F1 (Pr1214) Region: insert mid Sequence file: RDB19612_B2Ead.seq
>D08836A8_B2Ea_1_EV_linker_F1_G08_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NGGNNCGCNN GTGGNNCTGC CGGGGGGTCT GGAAGTGCAG    61 GTGGTAGCGC TGGAGGGTCC ACATCTGCAG GTGGGTCTGC TGGCGGTTCC GCCGGAGGGA   121 GTGCCGGCGG TAGCGCCGGT GGGAAGCTTA TGGTGAGTAC AGGGGAGGAG CTGTTCACCG   181 GGGTGGTGCC ATTTAAATTT CAACTTAAAG GAACCATCAA TGGCAAATCG TTTACCGTTG   241 AAGGCGAAGG TGAAGGAAAT TCACATGAAG GTTCTCATAA AGGAAAATAT GTTTGTACAA   301 GTGGAAAACT ACCGATGTCA TGGGCAGCAC TTGGAACATC CTTTGGTTAT GGAATGAAAT   361 ATTATACCAA ATATCCTAGT GGACTGAAGA ACTGGTTTCA TGAAGTAATG CCTGAAGGCT   421 TTACCTACGA TCGTCATATT CAATATAAAG GCGATGGGAG TATCCATGCA AAACACCAAC   481 ACTTTATGAA AAATGGGACT TATCACAACA TTGTTGAATT TACTGGTCAG GATTTTAAAG   541 AAAATAGTCC AGTCTTAACT GGAGATATGA ATGTCTCATT ACCGAATGAA GTTCAACATA   601 TACCCAGAGA TGATGGAGTA GAATGCCCAG TGACCTTGCT TTATCCTTTA TTATCGGATA   661 AATCAAAATG CGTTGAGGCT TACCAAAATA CAATCATCAA GCCTCTTCAT AATCAACCAG   721 CACCTGATGT CCCATATCAC TGGATTCGTA AACAATACAC ACAAAGCAAA GATGANACCG   781 AGGAACGTGA TCATATTATT CAATCAGAGA CTCTCGAAGC ACACTTACCT TGGCATGANC   841 CTTCAGCATC GGCAGTANGA TCCNNNGGGC TGCANGAATT CNATGGTGGA NGCGGNTCNN   901 NCGGANGNGG CTCTGGCGGN GGCGGNNCNN TCAAGCTTAT CGATANCGTC NANCNCNANG   961 GGGGGCCCGG NACCCANCTT TTGNTCCNNT TNNTGANGGN TNNNNTGNNN NCTTNNNNNN 1021 NNCNNNGNNC NNANCNNNTN NNNNNNNNN //
Primer: pBR322-ori-R (Pr1218) Region: pBR322 ori 3',AmpR Sequence file: RDB19612_B2Eae.seq
>D08836A8_B2Ea_1-pBR322-ori-R_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNGGNA GCTCTTGNNC CGGCAAACNA ACCACCGCTG GTAGCGGTGG    61 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT   121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT   181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA   241 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA   301 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT   361 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG   421 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA   481 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA   541 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG   601 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC   661 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC   721 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA   781 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGANTGGTG AGTACTCAAC   841 CAAGTCATTC TGANAATAGT GTATGCGGCG ANCGANTTGC TCTTGNCNNG CGTCAATACN   901 GGATAATACC GCGCCNCATA NCANAANTTT NAAANNGCTC ATCATTGGAA AANGNTCTTC   961 GGGGCGAAAA NNCTCNNNNN NNNNANNNCT NNNGNNNTNC NNTTCGATGT AACCCNNNNN 1021 NNNNN //
Primer: f1ori_F (Pr0280) Region: f1 ori,AmpR pro,AmpR Sequence file: RDB19612_B2Eaf.seq
>D08836A8_B2Ea_1-f1ori_F_C05_08_ABI24.ab1     1 NNNNNNNNNN NGNGNNNGNA GNGNAGCGGT CNNGCTGCGC GTAACCNCCA CACCCGCCGC    61 GCTTAATGCG CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC   121 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC   181 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT   241 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT   301 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA   361 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG   421 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA   481 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA   541 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG   601 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC   661 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA   721 TGAAGCCATA CCAAACGACG AGCGTGNCAC CACGATGCCT GTAGCAATGG CAACAACGTT   781 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACNAT TAATAGACTG   841 GATGGAGGCG GATAAAGTTG CANGACCACT TCTGCGCTCG GNCCTTNCNG CTGGNTGGNT   901 TANTGNTGAT AAATCTGGAN CCNGNNANCG TGGNNCTCGC GNNATCATTG CAGCACTNGG   961 GNNNGANNGT AAGCCNNNCC GTATCGNANT NNTCNACNCN NNNGGNNNNN NNNNANNTAN 1021 GGNNGNNNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl