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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)2.2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Catalog number RDB15454
Resource name tFucci(CA)2.2/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC212778 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
UCSD's MTA is also required. Please visit Additional Forms section.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15454 tFucci(CA)2.2/pCSII-CMV DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16942_A9Ctp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R
Sequence file: RDB16942_A9Cta.seq check
>15454_16942_A9Ct_mCherry-C_R_A01_01_ABI24.ab1
    1 CGTGGGGGTA GTCACTTGAT GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG
   61 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC
  121 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC
  181 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC
  241 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG
  301 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC
  361 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA
  421 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG
  481 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG
  541 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA
  601 TCCTCCTCGC CCTTGCTCAC CATGGTGGAT CCTATGCTAG CGGATCTGAC GGTTCACTAA
  661 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
  721 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
  781 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
  841 ATTGATGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
  901 CTGCCAAGTA GGAAAGTCCC ATAAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT
  961 TACCCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGGCATA TGATACACTT GATGTACTGG
 1021 CCCAGTGGGC CAGTTTACCG TAAATACTTC AACCCATGAC GTCATGGGTA AAGTTCCTAT
1081 GCGTACTATG GGAAACAATA CGTTCATTTA TTTGA
//
Primer: mCherry-C_F
Sequence file: RDB16942_A9Ctb.seq check
>15454_16942_A9Ct_mCherry-C_F_B01_04_ABI24.ab1
    1 AAACCGGAGG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
   61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
  121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGATG
  181 GAGCAGCGCC GCGTCACCGA CTTCTTCGCG CGCCGCCGCC CCGGGCCCCC CCGCATCGCG
  241 CCGCCCAAGC TGGCCTGCCG CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGCC
  301 TCCGCTACCA GTGGCAGCCG CAAGCGCGCC CGCCCGCCCG CCGCCCCCGG ACGCGACCAG
  361 GCCAGGCCAC CGGCCCGCGC GGCCGCGCGG CTGTCGGTGG ACGAGGTTTC CAGCCCCAGT
  421 ACCCCCGAGG CCCCAGACAT CCCAGCCTGC CCTTCTCCGG GCCAGAAGAT AAAGAAAACC
  481 GGTCGCGCCA AGCGCGGCAG CGGCGCCACC AACTTCAGCC TGCTGAAGCA GGCCGGCGAC
  541 GTGGAGGAGA ACCCCGGCCC CCGCGAATTC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
  601 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
  661 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
  721 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGAGCTG GGGCGTGCAG
  781 TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
  841 GAAGGCTACG TCCAGGAGCG TACCATCTTC TTCAAAGGAC GACGGCAACT ACAAGACCCG
  901 CGCCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAAACC GCATCGAGCT GAAGGGCATC
  961 GACTTCAAGA GGACGGCAAC ATCCCTGGGG CACAAGCTGG AGTACACTAC ATCAGCGGAC
 1021 AACGTCCTAT ATCACGGCGA CCAGGCAGAA GACCGCATCA AGCAAACCTT CCAGATCCGT
1081 CACACATTCG AGGAACGGCG CCGGTGCAGC CTCGGCTCGG AGCA
//
Primer: PRE_rev
Sequence file: RDB16942_A9Ctc.seq check
>15454_16942_A9Ct_PRE_rev_C01_07_ABI24.ab1
    1 TCGAATCCCC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
  121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
  181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
  241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
  301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
  361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
  421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
  481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
  541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGGTG CTCAGGTAGT
  601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG
  661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT
  721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTCGCTGAT GTAGTTGTAC TCCAGCTTGT
  781 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
  841 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA
  901 AGATGGTACG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT
  961 TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCACGCCCCA GCTCAGGTGG GTCACGATGA
 1021 TGGCCAGGCA CGGGCAGCTT GCCCGGTGTT GCAGATGGAA CTTTCATGGC TCAGCTGCGT
1081 TAAGTTGACA TTCGACCGTC GATCCCTTCG ACCGGGAC
//
Primer: HIVgp_R1
Sequence file: RDB16942_A9Ctd.seq check
>15454_16942_A9Ct_HIVgp_R1_D01_10_ABI24.ab1
    1 TTTCCCCCCA TGCCTCTTTT TTCTCTCTGC ACCACTCTTC TCTTTGCCTT GGTGGGTGCT
   61 ACTCCTAATG GTTCAATTTT TACTACTTTA TATTTATATA ATTCACTTCT CCAATTGTCC
  121 CTCATATCTC CTCCTCCAGG TCTGAAGATC AGCGGCCGGC CGCTTGCTGT GCGGTGGTCT
  181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA
  241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG
  301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT
  361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT
  421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA
  481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG
  541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG
  601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT
  661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
  721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
  781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  841 CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCAC CGTACACGCC
  901 TACCGCCCAT TTGCGTCAAT GGGGGCGGAG TTGTTACGAC ATTTTGGGAA AGTCCCGTTG
  961 ATTTTGGTGC CAAACAACTC CCATTGACGT CAATGGGGGT GAGACTTGGG AAATCCCCGT
 1021 GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCGAA ACGGCATCAC CATCTATAGC
1081 GATGACTATA CGTAGATGTA CTGCCTAGTA AGAAGTCCAA TAAGGGTTCC AATGGTA
//
Primer: BGH_rev2
Sequence file: RDB16942_A9Cte.seq check
>15454_16942_A9Ct_BGH_rev2_E01_13_ABI24.ab1
    1 TTCTTAGGCA ACGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA
  301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT
  361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT
  421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC
  481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA
  541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT
  601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA
  661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT
  721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC
  781 GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAAGCGAA
  841 GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GCCGCGGGAA AGGAAGGTCC GCTGGATTGA
  901 GGGCCGAAAG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA
  961 GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACACG GATGTCAGTG CCCACAGCGA
 1021 GCCCCTGTCA GCAGCGGGCA AGGCAAGGCC GCCGAATGAG TCGTCGTGCA TAGAGGGGAA
1081 GCGAAAGGTC CGGAAAGGGA GCTTG
//
Primer: SV40pro_F
Sequence file: RDB16942_A9Ctf.seq check
>15454_16942_A9Ct_SV40pro_F_F01_16_ABI24.ab1
    1 CATTGGTCGT TGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
  121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
  181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
  241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
  301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
  361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
  421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
  481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
  541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
  661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
  841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
  901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
  961 TAAATTGCGT TTGCGCTCAC TGCCCGCTTT CCCAGTCGGG AAAACCTGTC GTGCCCAGCT
 1021 GCATTTAAAT GATCGGGCCA AACGCGCGGG CAGAGCGATT GCGTAATGAG CGCTCATCGG
1081 CTCCTCGCTC ACTGGACTCG CTGGCGCCTC GGATCGAATC
//
Primer: env_F
Sequence file: RDB16942_A9Ctg.seq check
>15454_16942_A9Ct_env_F_G01_19_ABI24.ab1
    1 CCAGTATCGC ATTAGTGAAC GGATCTACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA
   61 AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC
  121 ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC
  181 AGGGACAGCA GAAATTCACT TTGAATTAAT TCGATTAATA GTAATCAATT ACGGGGTCAT
  241 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
  301 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
  361 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  421 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA
  481 AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT
  541 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG
  601 GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG
  661 GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  721 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT
  781 TAGTGAACCG TCAGATCCGC TAGCATAGGA TCCACCATGG TGAGCAAGGG CGAGGAGGAT
  841 AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC
  901 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC
  961 GCCCAAGCTG AATGTGACCA AGGGTGGCCC CCTGCCCTTT CGCTGGACAT CCCTGTCCCT
 1021 CTCAGTTCAT GTACGCTCCA GCCTACGTGG AGCACCCGCG ACTCCCCGAC TACTTGAGCT
1081 GTCCATCCCC GAGGGCTTCC AAGTTGGAGA AGCCGCCGTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl