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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)2.2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Catalog number RDB15454
Resource name tFucci(CA)2.2/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC212778 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15454 tFucci(CA)2.2/pCSII-CMV DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16942_A9Ctp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: mCherry-C_R
        Sequence file: RDB16942_A9Cta.seq check
        >15454_16942_A9Ct_mCherry-C_R_A01_01_ABI24.ab1
            1 CGTGGGGGTA GTCACTTGAT GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG
           61 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC
          121 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC
          181 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC
          241 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG
          301 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC
          361 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA
          421 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG
          481 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG
          541 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA
          601 TCCTCCTCGC CCTTGCTCAC CATGGTGGAT CCTATGCTAG CGGATCTGAC GGTTCACTAA
          661 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
          721 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
          781 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
          841 ATTGATGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
          901 CTGCCAAGTA GGAAAGTCCC ATAAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT
          961 TACCCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGGCATA TGATACACTT GATGTACTGG
         1021 CCCAGTGGGC CAGTTTACCG TAAATACTTC AACCCATGAC GTCATGGGTA AAGTTCCTAT
        1081 GCGTACTATG GGAAACAATA CGTTCATTTA TTTGA
        //
        Primer: mCherry-C_F
        Sequence file: RDB16942_A9Ctb.seq check
        >15454_16942_A9Ct_mCherry-C_F_B01_04_ABI24.ab1
            1 AAACCGGAGG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
           61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
          121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGATG
          181 GAGCAGCGCC GCGTCACCGA CTTCTTCGCG CGCCGCCGCC CCGGGCCCCC CCGCATCGCG
          241 CCGCCCAAGC TGGCCTGCCG CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGCC
          301 TCCGCTACCA GTGGCAGCCG CAAGCGCGCC CGCCCGCCCG CCGCCCCCGG ACGCGACCAG
          361 GCCAGGCCAC CGGCCCGCGC GGCCGCGCGG CTGTCGGTGG ACGAGGTTTC CAGCCCCAGT
          421 ACCCCCGAGG CCCCAGACAT CCCAGCCTGC CCTTCTCCGG GCCAGAAGAT AAAGAAAACC
          481 GGTCGCGCCA AGCGCGGCAG CGGCGCCACC AACTTCAGCC TGCTGAAGCA GGCCGGCGAC
          541 GTGGAGGAGA ACCCCGGCCC CCGCGAATTC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
          601 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
          661 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
          721 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGAGCTG GGGCGTGCAG
          781 TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
          841 GAAGGCTACG TCCAGGAGCG TACCATCTTC TTCAAAGGAC GACGGCAACT ACAAGACCCG
          901 CGCCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAAACC GCATCGAGCT GAAGGGCATC
          961 GACTTCAAGA GGACGGCAAC ATCCCTGGGG CACAAGCTGG AGTACACTAC ATCAGCGGAC
         1021 AACGTCCTAT ATCACGGCGA CCAGGCAGAA GACCGCATCA AGCAAACCTT CCAGATCCGT
        1081 CACACATTCG AGGAACGGCG CCGGTGCAGC CTCGGCTCGG AGCA
        //
        Primer: PRE_rev
        Sequence file: RDB16942_A9Ctc.seq check
        >15454_16942_A9Ct_PRE_rev_C01_07_ABI24.ab1
            1 TCGAATCCCC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
           61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT
          121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT
          181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT
          241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC
          301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG
          361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT
          421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC
          481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
          541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGGTG CTCAGGTAGT
          601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG
          661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT
          721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTCGCTGAT GTAGTTGTAC TCCAGCTTGT
          781 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
          841 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA
          901 AGATGGTACG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT
          961 TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCACGCCCCA GCTCAGGTGG GTCACGATGA
         1021 TGGCCAGGCA CGGGCAGCTT GCCCGGTGTT GCAGATGGAA CTTTCATGGC TCAGCTGCGT
        1081 TAAGTTGACA TTCGACCGTC GATCCCTTCG ACCGGGAC
        //
        Primer: HIVgp_R1
        Sequence file: RDB16942_A9Ctd.seq check
        >15454_16942_A9Ct_HIVgp_R1_D01_10_ABI24.ab1
            1 TTTCCCCCCA TGCCTCTTTT TTCTCTCTGC ACCACTCTTC TCTTTGCCTT GGTGGGTGCT
           61 ACTCCTAATG GTTCAATTTT TACTACTTTA TATTTATATA ATTCACTTCT CCAATTGTCC
          121 CTCATATCTC CTCCTCCAGG TCTGAAGATC AGCGGCCGGC CGCTTGCTGT GCGGTGGTCT
          181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA
          241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG
          301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT
          361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT
          421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA
          481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG
          541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG
          601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT
          661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
          721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
          781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
          841 CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCAC CGTACACGCC
          901 TACCGCCCAT TTGCGTCAAT GGGGGCGGAG TTGTTACGAC ATTTTGGGAA AGTCCCGTTG
          961 ATTTTGGTGC CAAACAACTC CCATTGACGT CAATGGGGGT GAGACTTGGG AAATCCCCGT
         1021 GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCGAA ACGGCATCAC CATCTATAGC
        1081 GATGACTATA CGTAGATGTA CTGCCTAGTA AGAAGTCCAA TAAGGGTTCC AATGGTA
        //
        Primer: BGH_rev2
        Sequence file: RDB16942_A9Cte.seq check
        >15454_16942_A9Ct_BGH_rev2_E01_13_ABI24.ab1
            1 TTCTTAGGCA ACGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
           61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
          121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
          181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
          241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA
          301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT
          361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT
          421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC
          481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA
          541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT
          601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA
          661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT
          721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC
          781 GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAAGCGAA
          841 GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GCCGCGGGAA AGGAAGGTCC GCTGGATTGA
          901 GGGCCGAAAG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA
          961 GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACACG GATGTCAGTG CCCACAGCGA
         1021 GCCCCTGTCA GCAGCGGGCA AGGCAAGGCC GCCGAATGAG TCGTCGTGCA TAGAGGGGAA
        1081 GCGAAAGGTC CGGAAAGGGA GCTTG
        //
        Primer: SV40pro_F
        Sequence file: RDB16942_A9Ctf.seq check
        >15454_16942_A9Ct_SV40pro_F_F01_16_ABI24.ab1
            1 CATTGGTCGT TGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
           61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
          121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
          181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
          241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
          301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
          361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
          421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
          481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
          541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
          601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
          661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
          721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
          781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
          841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
          901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
          961 TAAATTGCGT TTGCGCTCAC TGCCCGCTTT CCCAGTCGGG AAAACCTGTC GTGCCCAGCT
         1021 GCATTTAAAT GATCGGGCCA AACGCGCGGG CAGAGCGATT GCGTAATGAG CGCTCATCGG
        1081 CTCCTCGCTC ACTGGACTCG CTGGCGCCTC GGATCGAATC
        //
        Primer: env_F
        Sequence file: RDB16942_A9Ctg.seq check
        >15454_16942_A9Ct_env_F_G01_19_ABI24.ab1
            1 CCAGTATCGC ATTAGTGAAC GGATCTACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA
           61 AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC
          121 ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC
          181 AGGGACAGCA GAAATTCACT TTGAATTAAT TCGATTAATA GTAATCAATT ACGGGGTCAT
          241 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
          301 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
          361 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
          421 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA
          481 AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT
          541 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG
          601 GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG
          661 GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
          721 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT
          781 TAGTGAACCG TCAGATCCGC TAGCATAGGA TCCACCATGG TGAGCAAGGG CGAGGAGGAT
          841 AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC
          901 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC
          961 GCCCAAGCTG AATGTGACCA AGGGTGGCCC CCTGCCCTTT CGCTGGACAT CCCTGTCCCT
         1021 CTCAGTTCAT GTACGCTCCA GCCTACGTGG AGCACCCGCG ACTCCCCGAC TACTTGAGCT
        1081 GTCCATCCCC GAGGGCTTCC AAGTTGGAGA AGCCGCCGTG
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl