Resource data sheet
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tFucci(CA)2.2/pCSII-CMV

Lentivirus vector construct of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Catalog number RDB15454
Resource name tFucci(CA)2.2/pCSII-CMV
Clone info. Lentivirus expression vector of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC212778 (DDBJ accession number)
Originator Miyawaki, Atsushi |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15454 tFucci(CA)2.2/pCSII-CMV DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15454_A7Lip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R RDB15454_A7Lia.seq
>D05018B5_4_A7Li_mCherry-C_R_D03_12_ABI24.ab1
    1 TTGGGAGGTA GTCACTTGAT GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG
   61 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC
  121 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC
  181 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC
  241 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG
  301 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC
  361 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA
  421 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG
  481 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG
  541 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA
  601 TCCTCCTCGC CCTTGCTCAC CATGGTGGAT CCTATGCTAG CGGATCTGAC GGTTCACTAA
  661 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
  721 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
  781 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
  841 ATTGATGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
  901 CTGCCCAAGT AGGAAAGTCC CATAAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT
  961 TTACCCGTCA TTGACGTCAA TAGGGCGTAC TTGGCATATG ATACACTTGG ATGTACCTAC
 1021 TAGGTGGGGC AGATTTACGG TTAAATACTC CACCCATTGA CGTTCAATGG AAGTCCTAAT
1081 GACGATTACT TATTG
//
Primer: mCherry-C_F RDB15454_A7Lib.seq
>D05018B5_4_A7Li_mCherry-C_F_B01_02_ABI08.ab1
    1 GCACGAAAGC CCGGTTGCAG CTGCCCGGCG CCTACAACGT CAAACATCAA GTTGGACATC
   61 ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC
  121 TCCACCGGCG GCATGGACGA GCTGTACAAG GGATATCCAT CACACTGGCG GCCGCTCGAG
  181 ATGGAGCAGC GCCGCGTCAC CGACTTCTTC GCGCGCCGCC GCCCCGGGCC CCCCCGCATC
  241 GCGCCGCCCA AGCTGGCCTG CCGCACCCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG
  301 GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC
  361 CAGGCCAGGC CACCGGCCCG CGCGGCCGCG CGGCTGTCGG TGGACGAGGT TTCCAGCCCC
  421 AGTACCCCCG AGGCCCCAGA CATCCCAGCC TGCCCTTCTC CGGGCCAGAA GATAAAGAAA
  481 ACCGGTCGCG CCAAGCGCGG CAGCGGCGCC ACCAACTTCA GCCTGCTGAA GCAGGCCGGC
  541 GACGTGGAGG AGAACCCCGG CCCCCGCGAA TTCATGGTGA GCAAGGGCGA GGAGCTGTTC
  601 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC
  661 GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC
  721 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAG CTGGGGCGTG
  781 CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG
  841 CCCGAAGGCT ACGTCCAGGG AGCGTACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC
  901 CCGCGCCGAG GTGAAGTTTC GAGGGCGACA CCCTGGTGAA ACCGCATCGA GCTGAAGGGC
  961 AATCGACTTT CAGGAGGACG GCAACATCCT TGGGGGCACA AGCTTGGGAG TACAACTTAC
 1021 ATTCAGCGGA CAACGTCTAT ATCACCCGCC CGAACAAGGC AGAAGGCGGG CAATCAACGT
1081 CACCTTCCAG GATTCCGCTC AACTAACCAA TCGAAAGGGA ACGGGCCG
//
Primer: PRE_rev RDB15454_A7Lic.seq
>D05018B5_4_A7Li_PRE_rev_C01_03_ABI08.ab1
    1 AGGAAATCCC CATTAGCCGG TAAAAGGGAA GCAACATAGG TTAAGAATAC CAGTCAAATC
   61 TTTCAACAAA TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA
  121 CTCTAGATTA CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG
  181 GATTTTCTTT AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT
  241 CACTAGATTC TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC
  301 GATGTTTCCT TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG
  361 ATGCAGAAGG CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT
  421 CTTTGATTTC TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG
  481 ATGGATATCC CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT
  541 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGGTGC
  601 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT
  661 AGTGGTCGGC GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTGGCCT
  721 TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTCGCTGATG TAGTTGTACT
  781 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC
  841 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTTCG
  901 TCCTTGAAGA AGATGGTACG CTCCCTGGAC GTAGCCTTTC GGGCATGGCG GGACTTGAAA
  961 GAAGTTCGTG CCTGCTTCAT GTGGGTCGGG GGTAGCGGGC GGAGCAACTG CAACGCCTCT
 1021 AGCTCAGGGT GGGTCACGAG GGGGTGGGTC CAGGGCCACG GGCAAGCTTG CCGGTGGGTG
 1081 CAGAATGGAA CTTCAGGATC AGTCTAGGCT CGTTAGGGAG TGCCAATTTC GGTCTCTATC
1141 CGCGCTCT
//
Primer: HIVgp_R1 RDB15454_A7Lid.seq
>D05018B5_4_A7Li_HIVgp_R1_D01_04_ABI08.ab1
    1 TGGGCATCCT ATTCCCACTG CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT
   61 GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA
  121 ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGGCCGC TTGCTGTGCG
  181 GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT
  241 ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT
  301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT
  361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT
  421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC
  481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT
  541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC
  601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG
  661 TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC
  721 TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC
  781 TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT
  841 GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC CTCCCACCGT
  901 ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGGAAAGTC
  961 CCGGTTGATT TTTGGTGCCA AAACAAAACT CCCATTGAAC GTCAATGGGG GTGGGAGAAC
 1021 TTGGGAAATT CCCCGGTGAG TCAACCGCTA TTCCACGCCC AATTTGAATG GTACTGCTAA
 1081 AACCCGGCAA TTCAACATTG TTAATTAGGC GAATGAACTA ATAACGGTTA GAGATGTGAT
1141 AACTGTGGCT AG
//
Primer: BGH_rev2 RDB15454_A7Lie.seq
>D05018B5_4_A7Li_BGH_rev2_E01_05_ABI08.ab1
    1 TGTTACAAGG GTCCAGGTAG CACGGGGGAG GGGCAAGCAA CAGATGGCTG GCAGACTAGA
   61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG
  121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA
  181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG
  241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT
  301 CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT
  361 CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT
  421 TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA
  481 GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG
  541 TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  601 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  661 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  721 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  781 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC
  841 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAAGGTCC
  901 GCTGGATTGA GGGCCGAAAG GGACGTAGCA GAAAGGACGT CCCGCGCAGA ATCCCAGGGT
  961 GGCAACACAG GCGAGCAGCC ATGGGAAAGG ACGTTCAGCT TCCCCCGACA CACCACGGGA
 1021 AATTGTCAGT GCCCAACAGC CGGAGCCCCC TGTCCAGCAG CGGGCAGGCA AGGCGGCCGA
1081 TGATCCGTCG TGCATAGGAA GGGGGAAAGC CGAAAAAGG
//
Primer: SV40pro_F RDB15454_A7Lif.seq
>D05018B5_4_A7Li_SV40pro_F_F01_06_ABI08.ab1
    1 CTTCGGGGCG TGTAACTAAT CCGGAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
  121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
  181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA
  241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT
  301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA
  361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT
  421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC
  481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA
  541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
  601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
  661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
  721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
  781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
  841 GGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAATTGT TATCCGCTCA CAATTCCACA
  901 CAACATACGA GCCGGGAAGC ATAAAGTGTA AAGCCTGGGG TGCTCTAATG AAGTGAGCTA
  961 ACTCACATTT AATTGCGTTG CGCTCACTGC CCGCCTTTTC CAGTCGGGAA ACCTGGTCGT
 1021 GCCAGCTGCA TTTAATGAAT CGGCCAACGC CGCCGGGAGA GGCCGGTTTG GCGTAATTGG
1081 GCGCTCATCC GGCCATCCTT CCGCTTCAAC TGGAACTTCC GCCTTGTCGG TCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2018.10.09

GNP_filter3_RDBDEP_html_180823.pl