RDB20222 - pcDNA3/td5StayGold(c4)=GianCreg

pcDNA3/td5StayGold(c4)=GianCreg (#RDB20222)

Expression vector of td5StayGold for labeling the Golgi apparatus.

Drawn by SnapGene® software

Sequence (full)	RDB20222hts01.seq
Publication	Ando, R., Nat. Methods 21 (4): 648-656 (2024). [link to RRC of NBRP]
Test sheet	RDB20222_B3L4p1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	Expression vector of td5StayGold for labeling the Golgi apparatus. c4 is the appendage for C-terminal tagging with td5StayGold. 'GianCreg' is an abbreviation for giantin C-terminal region. '=' in the resource name denotes a linker [(GGGGS)3].
Vector backbone	pcDNA3 (plasmid)
Gene/insert name	Cytaeis uchidae GFP (tdStayGold); human giantin C-terminal region (GOLGB1).
Depositor\|Developer	Miyawaki, Atsushi \| Ando, Ryoko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando, R. et al. Nat. Methods 21 (4): 648-656 (2024).
4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando, R. et al. Nat. Methods 21 (4): 648-656 (2024). 4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando, R. et al. Nat. Methods 21 (4): 648-656 (2024). 4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB20222	pcDNA3/td5StayGold(c4)=GianCreg	DNA solution

How to cite this biological resource

Materials & Methods section:

The pcDNA3/td5StayGold(c4)=GianCreg was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20222).

Reference section:

Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

human GOLGB1

External Database
Cytaeis uchidae GFP

human GOLGB1

Reference sequence