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tFucci(SA)2.1/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). CMV promoter.

Catalog number RDB15450
Resource name tFucci(SA)2.1/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of insert sequence LC192887 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Anemonia majano CFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
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        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15450 tFucci(SA)2.1/pCSII-CMV DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15450_A7Jvp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: mCherry-C_R
        Region: insert 5'(mCherry 5'),CMV pro.2
        Sequence file: RDB15450_A7Jvg.seq check
        >D05018B1_1_A7Jv_mCherry-C_R_F01_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNGTTGNNGT TGNAGGCGCC GGGCAGCTGC ACGGGCTTCT
           61 TGGCCTTGTA GGTGGTCTTG ACCTCAGCGT CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC
          121 TCTGCTTGAT CTCGCCCTTC AGGGCGCCGT CCTCGGGGTA CATCCGCTCG GAGGAGGCCT
          181 CCCAGCCCAT GGTCTTCTTC TGCATTACGG GGCCGTCGGA GGGGAAGTTG GTGCCGCGCA
          241 GCTTCACCTT GTAGATGAAC TCGCCGTCCT GCAGGGAGGA GTCCTGGGTC ACGGTCACCA
          301 CGCCGCCGTC CTCGAAGTTC ATCACGCGCT CCCACTTGAA GCCCTCGGGG AAGGACAGCT
          361 TCAAGTAGTC GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT
          421 GAGGGGACAG GATGTCCCAG GCGAAGGGCA GGGGGCCACC CTTGGTCACC TTCAGCTTGG
          481 CGGTCTGGGT GCCCTCGTAG GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC
          541 CGTTCACGGA GCCCTCCATG TGCACCTTGA AGCGCATGAA CTCCTTGATG ATGGCCATGT
          601 TATCCTCCTC GCCCTTGCTC ACCATGGTGG ATCCTATGCT AGCGGATCTG ACGGTTCACT
          661 AAACCAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT
          721 GGGGCGGANT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC
          781 CCATTGACGT CNATGGGGTG GAGACTTGGA AATCCCCGTG ANTCNAACCG CTATCCACGC
          841 CNATTGATGT ACTGCCAAAA CCGCATCACC ATGGNANTAG CGATGANTAA TACGTAGATG
          901 TACTGCCNAG TANGAAANNC CCNTAAGGTC ATGTACTGNN NATAATGCCN NNCNGGNCAT
          961 TTANNGTCAT NNACNNCNAT NNNGGCGNAN NTNGNCATAT GANACACTTN NNGNNNTGNC
        1021 NAANTGGNNN NTNNNNGNAA ANNCTNCNNN NNNNNNN
        //
        Primer: PRE_rev
        Region: PRE,insert 3'(hGem(1_110),AmCyan1 3')
        Sequence file: RDB15450_A7Jvh.seq check
        >D05018B1_1_A7Jv_PRE_rev_G01_19_ABI24.ab1
            1 NNNNNNNNNN NNNGNNNNGG ANNNNNNAGT TAAGAATACC AGTCAATCTT TCACAAATTT
           61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG ATGTTAACTC TAGATTACAG
          121 CGCCTTTCTC CGTTTTTCTG CCACTTCCTT CCAATACTGA GAGGATGGAT TTTCTTTAAT
          181 CATAAGATCA AATGACTCCT GGGTGACTCC TCCAAGATTT TTATTTTCAC TAGATTCTGG
          241 GACAATAACC CCAGGGCTGG AAGTTGTAGA TGTTAAGTGG TCATTCCGAT GTTTCCTTTT
          301 GGACAAGCCT GCGGACAGCT CATTTTCTCT TCCAACAAGA GATCCAGATG CAGAAGGCTG
          361 AATCATCTTC AGAGTTCTTC TTGGGACAGA ACTATTCTTT ATATTCTCTT TGATTTCTTC
          421 TTGTTTCTGC TTCATACTGG GATTCATCTC GAGCGGCCGC CAGTGTGATG GATATCCGAA
          481 GGGCACCACG GAGGTGATGT GGGCCACGGC GTGCTCGGTC AGCTGCACGC TGTTGCCGCC
          541 CTTGTCCAGG TCGGTTCTGG CGATGCGGTG CTCCACCACG TGGTTGGGGG GCATGGTCAC
          601 GGGCTTCTTG GTCTTGTAGG AGGTGTGGAA CTGGCATCTG TAGTTGCCGC CGCCCTGCAG
          661 CATCAGGAAG GCGGTCACGT CGCCCTTCAA GATGCCGTCG CACACGGTCA TCTTCTCGAA
          721 NGANGGGTCC CAGCCGGTGG TCTTCTTGGC CATCACGGGG CCGTCGGCGG GGAAGTTCAC
          781 GCCGNGNAAN GTGGANTTGT GCTCGAAGCA GTTGCCCTTC ANGCTGATCT CCCNGCTGGC
          841 GGTGGCCACG CCGCCNTCCN CGTNNGTGAA NGNTCTCTCG NANGANATGC CGNCNGGGAA
          901 NGNCNGCTTG AAGTANTCNG GNNTGCTNGT GGGGTNNNNG GNGAANCANC GGNNGNNGTA
        961 CNTGNNNNNN NNNN
        //
        Primer: mRFP1-inner1
        Region: insert mid(mCherry 3',hCdt1(30_120) 5')
        Sequence file: RDB15450_A7Jvi.seq check
        >15450_15450_B1K5F_1_mRFP1-inner1_E03_15_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCNN NGNNTCCTCC CTGCAGGANG GCGAGTTCAT CTACAAGGTG
           61 AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC
          121 TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG
          181 CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG
          241 GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
          301 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
          361 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGCCC
          421 AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC
          481 GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG CAGGANACTG
          541 CGGCTGTCGG TGGACGAGGT TTCCAGCCCC AGTACCCCCG ANGCCCCAGA CATCCCAGCC
          601 TGCCCTTCTC CGGGCCAGAA GANAAAGAAA TCCACCCCGG CAGCNNNNCA GCCGCCCCNC
          661 CTGANNTCCG CGCNNNNCNG NNNANNATCN CNNGNCGNGN NNNGCGCNNN ANCNGCNCNN
        721 NNNNNNNN
        //
        Primer: mCherry-C_F
        Region: insert mid(mCherry 3',hCdt1(30_120), P2A,AmiCyan1 5')
        Sequence file: RDB15450_A7Jvj.seq check
        >D05018B1_1_A7Jv_mCherry-C_F_A02_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCNG CCNGGCGCCT ACNACGTCNA CATCAAGTTG GNCATCACCT
           61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
          121 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA
          181 GCCCCGCCAG GCCCGCACTC CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG
          241 CCCGCCCGCC CGCCGCCCCC GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC
          301 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT
          361 GCCCTTCTCC GGGCCAGAAG ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC
          421 TGACATCCGC GCAGGACCAG GACACCATCA CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA
          481 CCAACTTCAG CCTGCTGAAG CAGGCCGGCG ACGTGGAGGA GAACCCCGGC CCCCGCGAAT
          541 TCGCCACCAT GGCCCTGTCC AACAAGTTCA TCGGCGACGA CATGAAGATG ACCTACCACA
          601 TGGACGGCTG CGTGAACGGC CACTACTTCA CCGTGAAGGG CGAGGGCAGC GGCAAGCCCT
          661 ACGAGGGCAC CCAGACCTCC ACCTTCAAGG TGACCATGGC CAACGGCGGC CCCCTGGCCT
          721 TCTCCTTCGA CATCCTGTCC ACCGTGTTCA TGTACGGCAA CCGCTGCTTC ACCGCCTACC
          781 CCACCAGCAT GCCCGANTAC TTCNAGCAGG CCTTCNNCGA CGGCATGTCC TACGAGAGAA
          841 NCTTCACCTA CNANGANGGC GGCGTGGCCA CCGCCAGCTG NGANNTNAGC CTGAANGGNA
          901 ACTGCTTCNA NCACNAGTNC ACCTTCCNNN GCGTNNNNTT CCCCGNCGAN NGNCCCNNGA
          961 TGNNNNAGAN NANCACNGNN TNGGANCNNN NCNTNNNANA ANNNGANNGN NNGNNNNNNN
        1021 NTNNNNNNNG NNNNNNNNNN
        //
        Primer: AmCyan1-H_F
        Region: insert mid(AmCyan1 mid)
        Sequence file: RDB15450_A7Jvk.seq check
        >15450_15450_B1K5F_1_HIV-B_R_C03_09_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNTTTGGC GTACTCNCCA GTCGCCGCCC CTCGCCTCTT
           61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC
          121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC
          181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC
          241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG
          301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC
          361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
          421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA
          481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC
          541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCNAGTAGG AANGTCCCAT
          601 AANGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAN CGTCNTTGAC GTCNATANGG
          661 GGGCGTACNN NGNATATGAT ACACTNGNNG TACTGNCNNN NGGGNNNNTN ANNNNNNNNN
        721 TNNNNCNNNN NNNNNN
        //
        Primer: HIV-B_R
        Region: HIV-1 psi,5'LTR, CMV pro.1, CMV en.1
        Sequence file: RDB15450_A7Jvl.seq check
        >15450_15450_B1K5F_1_HIV-B_R_C03_09_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNTTTGGC GTACTCNCCA GTCGCCGCCC CTCGCCTCTT
           61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC
          121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC
          181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC
          241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG
          301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC
          361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
          421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA
          481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC
          541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCNAGTAGG AANGTCCCAT
          601 AANGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAN CGTCNTTGAC GTCNATANGG
          661 GGGCGTACNN NGNATATGAT ACACTNGNNG TACTGNCNNN NGGGNNNNTN ANNNNNNNNN
        721 TNNNNCNNNN NNNNNN
        //
        Primer: HIVgp_R1
        Region: HIV-1 psi,5'LTR
        Sequence file: RDB15450_A7Jvm.seq check
        >D05018B1_1_A7Jv_HIVgp_R1_H01_22_ABI24.ab1
            1 NNNNNNNNNN NNNNNNGCNC TTTTTTCTCT CTGCACNNCT CTTCTCTTTG CCTTGGTGGG
           61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
          121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
          181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
          241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
          301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
          361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
          421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
          481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
          541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
          601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
          661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG ANGGANCTCT
          721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
          781 ATTGANGCTT AAGCAGTNNN TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
          841 CTAACCAGAG AGANCCAGTA CAGGCAAAAN GCGCTGCTTA TATAGANCTC CNANCGTACA
          901 CGCCNACCGC CNATTTGCGT CNNTGGGGNN NANTTGNNNC NANNNTTNNN AAANTCCCGT
          961 TNANTTTNGN GNCNAAACAA ANTNCNNTTN NNGTCNNNNG GGNNNNNNNT TNNNAANTCC
        1021 CNNNGANNNN NNNNNN
        //
        Primer: BGH_rev2
        Region: bGH pA,3'LTR,PRE
        Sequence file: RDB15450_A7Jvn.seq check
        >D05018B1_1_A7Jv_BGH_rev2_C01_07_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNGN NNNGGGGNNG GGNNNNNNNC AGATGGCTGG CAACTAGAAG
           61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC
          121 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT
          181 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG
          241 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT
          301 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC
          361 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG
          421 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT
          481 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG
          541 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
          601 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
          661 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
          721 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG
          781 TCTCGACGGT ATCGATGCGG GGANGCGGCC NNNAGGGANA TCCGANTCGT CTGNNGGCGA
          841 ANGCGAAGAN GCGGAANANG CCGCANANCC GGCAGCANGC CGNNGGAANG AANGNNCGCT
          901 GGANTGANGG NCNAANNGNA CNTNNCANAA NNNACNTNCN NCGCANAATC CANGNGGNNN
        961 CACAGGNNAN CAGNNNNNGN NAANGNANNN NNGNNNNNNN NNN
        //
        Primer: cPPT+CTS_V2_F
        Region: cPPT_CTS,CMV en.2
        Sequence file: RDB15450_A7Jvo.seq check
        >15450_15450_B1K5F_1_cPPT+CTS_V2_F_G03_21_ABI24.ab1
            1 NNNNNNNNNN NNNNCNNNNN NNNNNCAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT
           61 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG
          121 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
          181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC NTATGTTCCC
          241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
          301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
          361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
          421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC
          481 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGANTTC CAAGTCTCCA CCCCATTGAC
          541 GTCAATGGGA GTTTGTTTTG GCACCNAAAT CAACGGGACT TTCCAAAATG TCGTAACNAC
          601 TCCGCCCCAT TGACGCAAAT GGGCGGNANG CGTGTACGGT GGGANGTCTA TATAANCNGA
          661 NCTGGNTNAN TGNANNNNNN NANCCGCTNG NNTNNNANNC NNNNNNNNGN NNNNGGNNNA
        721 NGNNNNNNNN NN
        //
        Primer: SV40pro_F
        Region: Zeo,SV40 pA
        Sequence file: RDB15450_A7Jvp.seq check
        >D05018B1_1_A7Jv_SV40pro_F_C02_08_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNANNNGTG AGGANGNTTT TTTGGAGGCC TAGGCTTTTG
           61 CAAAAAGCTC CCGGGAGCTT GTATATCCNT TTTCGGATCT GATCAGCACG TGTTGACAAT
          121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
          181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
          241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
          301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
          361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
          421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
          481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
          541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
          601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
          661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
          721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCNC TGCATTCTAG TTGTGGTTTG
          781 TCCNAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG
          841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCNC AATTCCNCAC
          901 AACATACNAN CCGGAAGCAT AAAGTGTAAA NNCNGGGGNN GNNNAANGAG TGAGCTAACT
          961 CACNNNAATT GCGTTGCGCT CNCNGNCCGC TTNNCAGNNN NNAAACNNGN NNNGNNNNNN
        1021 NCNTTNNNGN NNNNGNCNNN NNNNNNGGNN NNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl