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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(SA)2.1/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). CMV promoter.

Catalog number RDB15450
Resource name tFucci(SA)2.1/pCSII-CMV
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of nucleotide sequence LC192887 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA (for use for not-for-profit academic purpose)[Link]
Remarks Remember that you will be working with samples containing infectious virus.
UCSD's MTA is also required. Please visit Additional Forms section.
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs).
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15450 tFucci(SA)2.1/pCSII-CMV DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15450_A7Jvp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R
Sequence file: RDB15450_A7Jva.seq check
>D0518B1_1_A7Jv_mCherry-C_R_F01_06_ABI08.ab1
    1 GTGGGGAAGT GAGTCACTTG ATGTTGACGT TGTAGGCGCC GGGCAGCTGC ACGGGCTTCT
   61 TGGCCTTGTA GGTGGTCTTG ACCTCAGCGT CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC
  121 TCTGCTTGAT CTCGCCCTTC AGGGCGCCGT CCTCGGGGTA CATCCGCTCG GAGGAGGCCT
  181 CCCAGCCCAT GGTCTTCTTC TGCATTACGG GGCCGTCGGA GGGGAAGTTG GTGCCGCGCA
  241 GCTTCACCTT GTAGATGAAC TCGCCGTCCT GCAGGGAGGA GTCCTGGGTC ACGGTCACCA
  301 CGCCGCCGTC CTCGAAGTTC ATCACGCGCT CCCACTTGAA GCCCTCGGGG AAGGACAGCT
  361 TCAAGTAGTC GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT
  421 GAGGGGACAG GATGTCCCAG GCGAAGGGCA GGGGGCCACC CTTGGTCACC TTCAGCTTGG
  481 CGGTCTGGGT GCCCTCGTAG GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC
  541 CGTTCACGGA GCCCTCCATG TGCACCTTGA AGCGCATGAA CTCCTTGATG ATGGCCATGT
  601 TATCCTCCTC GCCCTTGCTC ACCATGGTGG ATCCTATGCT AGCGGATCTG ACGGTTCACT
  661 AAACCAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT
  721 GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC
  781 CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC
  841 CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG
  901 TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT
  961 TTACCGTCAT TGACGTCAAT AGGGGCGTAC TTTGGCATAT GATACACTTG ATGTACTGCC
 1021 CAAGTGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAGTC CCTATGGCCG
 1081 TTACTATGGG AACATACGTC ATTATTGGAC GTCCATTGGC CGGGGTCGTG GGCGTTCAGG
1141 CAGGGCGGGG CCAATTTTAC CGGTTAAGGT TAATGTGTA
//
Primer: mCherry-C_F
Sequence file: RDB15450_A7Jvb.seq check
>D05018B1_1_A7Jv_mCherry-C_F_A02_02_ABI24.ab1
    1 TAAAAGCAAG CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
   61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA
  181 GCCCCGCCAG GCCCGCACTC CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG
  241 CCCGCCCGCC CGCCGCCCCC GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC
  301 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT
  361 GCCCTTCTCC GGGCCAGAAG ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC
  421 TGACATCCGC GCAGGACCAG GACACCATCA CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA
  481 CCAACTTCAG CCTGCTGAAG CAGGCCGGCG ACGTGGAGGA GAACCCCGGC CCCCGCGAAT
  541 TCGCCACCAT GGCCCTGTCC AACAAGTTCA TCGGCGACGA CATGAAGATG ACCTACCACA
  601 TGGACGGCTG CGTGAACGGC CACTACTTCA CCGTGAAGGG CGAGGGCAGC GGCAAGCCCT
  661 ACGAGGGCAC CCAGACCTCC ACCTTCAAGG TGACCATGGC CAACGGCGGC CCCCTGGCCT
  721 TCTCCTTCGA CATCCTGTCC ACCGTGTTCA TGTACGGCAA CCGCTGCTTC ACCGCCTACC
  781 CCACCAGCAT GCCCGACTAC TTCAAGCAGG CCTTCCCCGA CGGCATGTCC TACGAGAGAA
  841 CCTTCACCTA CGAGGACGGC GGCGTGGCCA CCGCCAGCTG GGAGATCAGC CTGAAGGGCA
  901 ACTGCTTCGA GCACAAGTCC ACCTTCCACG GCGTGAACTT CCCCGCCGAC GGCCCCGTGA
  961 TGGCCAAGAA GACCACCGGC TGGGACCCCT CCCTTCGAGA AGATGACCGT GTGCGACGGC
 1021 ATCTTGAAGG GCGACGTGAC CGCCTTTCCT GATGCTGCAG GCGCGCACTA CAGATGCCAG
 1081 TTCCACACTC TACCAGAACA GAGCTCGTGA CCATGCCCCC ACACGTTGTT GAAGCACGCA
1141 TCGCCAGAAA CCGAACCTGG AACCAAGGGT G
//
Primer: PRE_rev
Sequence file: RDB15450_A7Jvc.seq check
>D05018B1_1_A7Jv_PRE_rev_G01_19_ABI24.ab1
    1 ACGATCACAT AGCGTAAAGG AGCACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT
   61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG ATGTTAACTC TAGATTACAG
  121 CGCCTTTCTC CGTTTTTCTG CCACTTCCTT CCAATACTGA GAGGATGGAT TTTCTTTAAT
  181 CATAAGATCA AATGACTCCT GGGTGACTCC TCCAAGATTT TTATTTTCAC TAGATTCTGG
  241 GACAATAACC CCAGGGCTGG AAGTTGTAGA TGTTAAGTGG TCATTCCGAT GTTTCCTTTT
  301 GGACAAGCCT GCGGACAGCT CATTTTCTCT TCCAACAAGA GATCCAGATG CAGAAGGCTG
  361 AATCATCTTC AGAGTTCTTC TTGGGACAGA ACTATTCTTT ATATTCTCTT TGATTTCTTC
  421 TTGTTTCTGC TTCATACTGG GATTCATCTC GAGCGGCCGC CAGTGTGATG GATATCCGAA
  481 GGGCACCACG GAGGTGATGT GGGCCACGGC GTGCTCGGTC AGCTGCACGC TGTTGCCGCC
  541 CTTGTCCAGG TCGGTTCTGG CGATGCGGTG CTCCACCACG TGGTTGGGGG GCATGGTCAC
  601 GGGCTTCTTG GTCTTGTAGG AGGTGTGGAA CTGGCATCTG TAGTTGCCGC CGCCCTGCAG
  661 CATCAGGAAG GCGGTCACGT CGCCCTTCAA GATGCCGTCG CACACGGTCA TCTTCTCGAA
  721 GGAGGGGTCC CAGCCGGTGG TCTTCTTGGC CATCACGGGG CCGTCGGCGG GGAAGTTCAC
  781 GCCGTGGAAG GTGGACTTGT GCTCGAAGCA GTTGCCCTTC AGGCTGATCT CCCAGCTGGC
  841 GGTGGCCACG CCGCCGTCCT CGTAGGTGAA GGTTCTCTCG TAGGACATGC CGTCGGGGAA
  901 GGCCTGCTTG AAGTAGTCGG GCATGCTGGT GGGGTAGGCG GTGAAGCAGC GGTTGCCGTA
  961 CATGAACACG GTGGACAGGA TGTCGAAGGA GAAGGCCAGG GGGCCGCCGT TGGCCATGGT
 1021 CACCTTTGAA GTGGAGGTCT GGGTGCCTCG TAGGGCTTGC CGCTGCCCTC GCCCTTCACG
 1081 GTGAAGTAGT GACGTCACGC AGCGTCCATG GTGGTAAGTC ATCCTCATGT CGTCGCCGAA
1141 TGGACTGATG GACAGGTCAT GGGGTGGGCG GAAA
//
Primer: HIVgp_R1
Sequence file: RDB15450_A7Jvd.seq check
>D05018B1_1_A7Jv_HIVgp_R1_H01_22_ABI24.ab1
    1 GAGGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
  901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT
  961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGTGGAGACT TGGAAAATCC
 1021 CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CAAACGCATC ACCATGGTAT
 1081 AGCGATGACT AATACGTAGA TGTACTGCCA GTAGGAAGGT CCATAGTTCA TGTACTGGGC
1141 ATAATGCCCA GGCGGGCCAA TTACCGGTCC AATTGTGAAA GCGG
//
Primer: BGH_rev2
Sequence file: RDB15450_A7Jve.seq check
>D05018B1_1_A7Jv_BGH_rev2_C01_07_ABI24.ab1
    1 CAAGCAACGA TAAGGAAGGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC
  121 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT
  181 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG
  241 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT
  301 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC
  361 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG
  421 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT
  481 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG
  541 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
  601 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
  661 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
  721 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG
  781 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGAGGGCGA
  841 AGGCGAAGAC GCGGAAGAGG CCGCAGAGCC GGCAGCAGGC CGCGGGAAGG AAGGTCCGCT
  901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA AGGACGTCCC GCGCAGAATC CAGGTGGCAA
  961 CACAGGCGAG CAGCCATGGG AAAGGGACGT CAGCTTCCCC GACAACACCA CGGGAATTGT
 1021 CAGTGCCCAA CAGCCCGAGC CCCTGTCAGC AGCGGGCAAG CAGCCGCGAT GAGTCGTCGT
1081 GGCATAGAGG GGGAAGCCGA AGGTCCCTGG AAAGGGAGCC TGGAACAAGG TTGGT
//
Primer: SV40pro_F
Sequence file: RDB15450_A7Jvf.seq check
>D05018B1_1_A7Jv_SV40pro_F_C02_08_ABI24.ab1
    1 CCTGGGTCCC CTGGAACATT CAGAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT
  121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
  181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
  241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
  301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
  361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
  421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
  481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
  541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
  601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
  661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
  721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
  781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG
  841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
  901 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGGT GCCTAATGAG TGAGCTAACT
  961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
 1021 GCATTAATGA ATCGGCCCAA CGCGCGGGGA GAAGGCGGTT TGCGTATTTG GCGCTCTTTC
 1081 CGCTTCCCTC GCTCCACTGA CTCGCTGCGC TCGGTCGCTC CGGCTGCCGC CGAGCCGCTA
1141 ATCAGGCTCC ACCTCAAAGG GCGGGTAATA TAACCGGTTA ATTCTATC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl