RDB15450 - tFucci(SA)2.1/pCSII-CMV

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tFucci(SA)2.1/pCSII-CMV (#RDB15450)

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). CMV promoter.

Clone info.	Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). CMV promoter.
Vector backbone	CSII-CMV-MCS (RDB04377) (plasmid)
Size of vector backbone	8.6 kb
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Anemonia majano CFP cDNA
Reference of insert sequence	LC192887 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Please visit Ordering instruction.[link]

Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Ordering instruction.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. MTAに書く付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15450	tFucci(SA)2.1/pCSII-CMV	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The tFucci(SA)2.1/pCSII-CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15450).

Reference section:

Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15450_A7Jvp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R (Pr0517) Region: insert 5'(mCherry 5'),CMV pro.2 Sequence file: RDB15450_A7Jvg.seq
>D05018B1_1_A7Jv_mCherry-C_R_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGTTGNNGT TGNAGGCGCC GGGCAGCTGC ACGGGCTTCT 61 TGGCCTTGTA GGTGGTCTTG ACCTCAGCGT CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC 121 TCTGCTTGAT CTCGCCCTTC AGGGCGCCGT CCTCGGGGTA CATCCGCTCG GAGGAGGCCT 181 CCCAGCCCAT GGTCTTCTTC TGCATTACGG GGCCGTCGGA GGGGAAGTTG GTGCCGCGCA 241 GCTTCACCTT GTAGATGAAC TCGCCGTCCT GCAGGGAGGA GTCCTGGGTC ACGGTCACCA 301 CGCCGCCGTC CTCGAAGTTC ATCACGCGCT CCCACTTGAA GCCCTCGGGG AAGGACAGCT 361 TCAAGTAGTC GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT 421 GAGGGGACAG GATGTCCCAG GCGAAGGGCA GGGGGCCACC CTTGGTCACC TTCAGCTTGG 481 CGGTCTGGGT GCCCTCGTAG GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC 541 CGTTCACGGA GCCCTCCATG TGCACCTTGA AGCGCATGAA CTCCTTGATG ATGGCCATGT 601 TATCCTCCTC GCCCTTGCTC ACCATGGTGG ATCCTATGCT AGCGGATCTG ACGGTTCACT 661 AAACCAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT 721 GGGGCGGANT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC 781 CCATTGACGT CNATGGGGTG GAGACTTGGA AATCCCCGTG ANTCNAACCG CTATCCACGC 841 CNATTGATGT ACTGCCAAAA CCGCATCACC ATGGNANTAG CGATGANTAA TACGTAGATG 901 TACTGCCNAG TANGAAANNC CCNTAAGGTC ATGTACTGNN NATAATGCCN NNCNGGNCAT 961 TTANNGTCAT NNACNNCNAT NNNGGCGNAN NTNGNCATAT GANACACTTN NNGNNNTGNC 1021 NAANTGGNNN NTNNNNGNAA ANNCTNCNNN NNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(hGem(1_110),AmCyan1 3') Sequence file: RDB15450_A7Jvh.seq
>D05018B1_1_A7Jv_PRE_rev_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNGNNNNGG ANNNNNNAGT TAAGAATACC AGTCAATCTT TCACAAATTT 61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG ATGTTAACTC TAGATTACAG 121 CGCCTTTCTC CGTTTTTCTG CCACTTCCTT CCAATACTGA GAGGATGGAT TTTCTTTAAT 181 CATAAGATCA AATGACTCCT GGGTGACTCC TCCAAGATTT TTATTTTCAC TAGATTCTGG 241 GACAATAACC CCAGGGCTGG AAGTTGTAGA TGTTAAGTGG TCATTCCGAT GTTTCCTTTT 301 GGACAAGCCT GCGGACAGCT CATTTTCTCT TCCAACAAGA GATCCAGATG CAGAAGGCTG 361 AATCATCTTC AGAGTTCTTC TTGGGACAGA ACTATTCTTT ATATTCTCTT TGATTTCTTC 421 TTGTTTCTGC TTCATACTGG GATTCATCTC GAGCGGCCGC CAGTGTGATG GATATCCGAA 481 GGGCACCACG GAGGTGATGT GGGCCACGGC GTGCTCGGTC AGCTGCACGC TGTTGCCGCC 541 CTTGTCCAGG TCGGTTCTGG CGATGCGGTG CTCCACCACG TGGTTGGGGG GCATGGTCAC 601 GGGCTTCTTG GTCTTGTAGG AGGTGTGGAA CTGGCATCTG TAGTTGCCGC CGCCCTGCAG 661 CATCAGGAAG GCGGTCACGT CGCCCTTCAA GATGCCGTCG CACACGGTCA TCTTCTCGAA 721 NGANGGGTCC CAGCCGGTGG TCTTCTTGGC CATCACGGGG CCGTCGGCGG GGAAGTTCAC 781 GCCGNGNAAN GTGGANTTGT GCTCGAAGCA GTTGCCCTTC ANGCTGATCT CCCNGCTGGC 841 GGTGGCCACG CCGCCNTCCN CGTNNGTGAA NGNTCTCTCG NANGANATGC CGNCNGGGAA 901 NGNCNGCTTG AAGTANTCNG GNNTGCTNGT GGGGTNNNNG GNGAANCANC GGNNGNNGTA 961 CNTGNNNNNN NNNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid(mCherry 3',hCdt1(30_120) 5') Sequence file: RDB15450_A7Jvi.seq
>15450_15450_B1K5F_1_mRFP1-inner1_E03_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNN NGNNTCCTCC CTGCAGGANG GCGAGTTCAT CTACAAGGTG 61 AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC 121 TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG 181 CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG 241 GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC 301 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC 361 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGCCC 421 AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC 481 GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG CAGGANACTG 541 CGGCTGTCGG TGGACGAGGT TTCCAGCCCC AGTACCCCCG ANGCCCCAGA CATCCCAGCC 601 TGCCCTTCTC CGGGCCAGAA GANAAAGAAA TCCACCCCGG CAGCNNNNCA GCCGCCCCNC 661 CTGANNTCCG CGCNNNNCNG NNNANNATCN CNNGNCGNGN NNNGCGCNNN ANCNGCNCNN 721 NNNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid(mCherry 3',hCdt1(30_120), P2A,AmiCyan1 5') Sequence file: RDB15450_A7Jvj.seq
>D05018B1_1_A7Jv_mCherry-C_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNG CCNGGCGCCT ACNACGTCNA CATCAAGTTG GNCATCACCT 61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA 121 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA 181 GCCCCGCCAG GCCCGCACTC CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG 241 CCCGCCCGCC CGCCGCCCCC GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC 301 GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT 361 GCCCTTCTCC GGGCCAGAAG ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC 421 TGACATCCGC GCAGGACCAG GACACCATCA CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA 481 CCAACTTCAG CCTGCTGAAG CAGGCCGGCG ACGTGGAGGA GAACCCCGGC CCCCGCGAAT 541 TCGCCACCAT GGCCCTGTCC AACAAGTTCA TCGGCGACGA CATGAAGATG ACCTACCACA 601 TGGACGGCTG CGTGAACGGC CACTACTTCA CCGTGAAGGG CGAGGGCAGC GGCAAGCCCT 661 ACGAGGGCAC CCAGACCTCC ACCTTCAAGG TGACCATGGC CAACGGCGGC CCCCTGGCCT 721 TCTCCTTCGA CATCCTGTCC ACCGTGTTCA TGTACGGCAA CCGCTGCTTC ACCGCCTACC 781 CCACCAGCAT GCCCGANTAC TTCNAGCAGG CCTTCNNCGA CGGCATGTCC TACGAGAGAA 841 NCTTCACCTA CNANGANGGC GGCGTGGCCA CCGCCAGCTG NGANNTNAGC CTGAANGGNA 901 ACTGCTTCNA NCACNAGTNC ACCTTCCNNN GCGTNNNNTT CCCCGNCGAN NGNCCCNNGA 961 TGNNNNAGAN NANCACNGNN TNGGANCNNN NCNTNNNANA ANNNGANNGN NNGNNNNNNN 1021 NTNNNNNNNG NNNNNNNNNN //
Primer: AmCyan1-H_F (Pr1071) Region: insert mid(AmCyan1 mid) Sequence file: RDB15450_A7Jvk.seq
>15450_15450_B1K5F_1_HIV-B_R_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTTTGGC GTACTCNCCA GTCGCCGCCC CTCGCCTCTT 61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC 121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC 181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC 241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG 301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC 361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT 421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA 481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC 541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCNAGTAGG AANGTCCCAT 601 AANGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAN CGTCNTTGAC GTCNATANGG 661 GGGCGTACNN NGNATATGAT ACACTNGNNG TACTGNCNNN NGGGNNNNTN ANNNNNNNNN 721 TNNNNCNNNN NNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi,5'LTR, CMV pro.1, CMV en.1 Sequence file: RDB15450_A7Jvl.seq
>15450_15450_B1K5F_1_HIV-B_R_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTTTGGC GTACTCNCCA GTCGCCGCCC CTCGCCTCTT 61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC 121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC 181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC 241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG 301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC 361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT 421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA 481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC 541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCNAGTAGG AANGTCCCAT 601 AANGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAN CGTCNTTGAC GTCNATANGG 661 GGGCGTACNN NGNATATGAT ACACTNGNNG TACTGNCNNN NGGGNNNNTN ANNNNNNNNN 721 TNNNNCNNNN NNNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5'LTR Sequence file: RDB15450_A7Jvm.seq
>D05018B1_1_A7Jv_HIVgp_R1_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNGCNC TTTTTTCTCT CTGCACNNCT CTTCTCTTTG CCTTGGTGGG 61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT 121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG 181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC 241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT 301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT 361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA 421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT 481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG 541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT 601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC 661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG ANGGANCTCT 721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT 781 ATTGANGCTT AAGCAGTNNN TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT 841 CTAACCAGAG AGANCCAGTA CAGGCAAAAN GCGCTGCTTA TATAGANCTC CNANCGTACA 901 CGCCNACCGC CNATTTGCGT CNNTGGGGNN NANTTGNNNC NANNNTTNNN AAANTCCCGT 961 TNANTTTNGN GNCNAAACAA ANTNCNNTTN NNGTCNNNNG GGNNNNNNNT TNNNAANTCC 1021 CNNNGANNNN NNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,3'LTR,PRE Sequence file: RDB15450_A7Jvn.seq
>D05018B1_1_A7Jv_BGH_rev2_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNGGGGNNG GGNNNNNNNC AGATGGCTGG CAACTAGAAG 61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC 121 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT 181 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG 241 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT 301 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC 361 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG 421 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT 481 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG 541 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT 601 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT 661 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG 721 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG 781 TCTCGACGGT ATCGATGCGG GGANGCGGCC NNNAGGGANA TCCGANTCGT CTGNNGGCGA 841 ANGCGAAGAN GCGGAANANG CCGCANANCC GGCAGCANGC CGNNGGAANG AANGNNCGCT 901 GGANTGANGG NCNAANNGNA CNTNNCANAA NNNACNTNCN NCGCANAATC CANGNGGNNN 961 CACAGGNNAN CAGNNNNNGN NAANGNANNN NNGNNNNNNN NNN //
Primer: cPPT+CTS_V2_F (Pr0303) Region: cPPT_CTS,CMV en.2 Sequence file: RDB15450_A7Jvo.seq
>15450_15450_B1K5F_1_cPPT+CTS_V2_F_G03_21_ABI24.ab1 1 NNNNNNNNNN NNNNCNNNNN NNNNNCAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT 61 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG 121 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC 181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC NTATGTTCCC 241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT 301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT 361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT 421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC 481 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGANTTC CAAGTCTCCA CCCCATTGAC 541 GTCAATGGGA GTTTGTTTTG GCACCNAAAT CAACGGGACT TTCCAAAATG TCGTAACNAC 601 TCCGCCCCAT TGACGCAAAT GGGCGGNANG CGTGTACGGT GGGANGTCTA TATAANCNGA 661 NCTGGNTNAN TGNANNNNNN NANCCGCTNG NNTNNNANNC NNNNNNNNGN NNNNGGNNNA 721 NGNNNNNNNN NN //
Primer: SV40pro_F (Pr0110) Region: Zeo,SV40 pA Sequence file: RDB15450_A7Jvp.seq
>D05018B1_1_A7Jv_SV40pro_F_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNANNNGTG AGGANGNTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAAAGCTC CCGGGAGCTT GTATATCCNT TTTCGGATCT GATCAGCACG TGTTGACAAT 121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG 181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG 241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG 301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC 361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC 421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG 481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG 541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG 601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG 661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC 721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCNC TGCATTCTAG TTGTGGTTTG 781 TCCNAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG 841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCNC AATTCCNCAC 901 AACATACNAN CCGGAAGCAT AAAGTGTAAA NNCNGGGGNN GNNNAANGAG TGAGCTAACT 961 CACNNNAATT GCGTTGCGCT CNCNGNCCGC TTNNCAGNNN NNAAACNNGN NNNGNNNNNN 1021 NCNTTNNNGN NNNNGNCNNN NNNNNNGGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

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Other resources in our bank

External Database
Human CDT1

Human Geminin

Discosoma sp. RFP

Anemonia majano CFP

Reference sequence