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pNPY-Venus-N1 (#RDB15118)

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Clone info. Venus fluorescent protein targeting localization in secretory dense-core granules of neurites. Mammalian expression. Refer Fig.2 of Nagai, T., Nat. Biotechnol. 20, 87-90, 2002.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kan^r
Growth conditions LB + Kan, 37oC
Gene/insert name Human NPY cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15118hts01.seq checkAssembled from experimentally sequenced data.
Publication Nagai, T., Nat. Biotechnol. 20 (1): 87-90 (2002). [link to RRC of NBRP]
Test sheet RDB15118_B0Epp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15118 pNPY-Venus-N1 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pNPY-Venus-N1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15118).

Reference section:

Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K., Miyawaki, A., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]
reference Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345.
reference Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140.
reference Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]