Please review the QC test results indicated by icon below as well as clone information before placing your order.

pNPY-Venus-N1

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
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提供条件	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15118_A8B8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward () Sequence file: RDB15118_A8B8a.seq
>15118_15118_A8B8_CMV-Forward_B01_02_ABI08.ab1     1 CTGTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC   121 TCAAGCTTAG CCACCATGCT AGGTAACAAG CGACTGGGGC TGTCCGGACT GACCCTCGCC   181 CTGTCCCTGC TCGTGTGCCT GGGTGCGCTG GCCGAGGCGT ACCCCTCCAA GCCGGACAAC   241 CCGGGCGAGG ACGCACCAGC GGAGGACATG GCCAGATACT ACTCGGCGCT GCGACACTAC   301 ATCAACCTCA TCACCAGGCA GAGATATGGA AAACGATCCA GCCCAGAGAC ACTGATTTCA   361 GACCTCTTGA TGAGAGAAAG CACAGAAAAT GTTCCCAGAA CTCGGCTTGA AGACCCTGCA   421 ATGTGGACGG TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG   481 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC   541 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG   601 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC   661 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG   721 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC   781 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG   841 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGG CACAAGCTGG AGTACAACTA   901 CAACAGCCAC AACGTCTATA TCACCGCCGA CAGCAGAAGA ACGGGCATCA AAGGCCCAAC   961 TTCAAGATCC GCCACAACAT CGAGGGACGG CGGCGTGCAG CTCGCCCGAC CACTACCAGC  1021 AGAACACCCC CCATCGGCGA CGGCCCCCGT TGCCTGGCTG CCCGGAACAA CCACCTAACC 1081 TTGAAGCCTT ACAAGTCCGG CCCTTGAGCA AAAGAAACCC CCAAAAACCG AGAAG //
Primer: ME1290Rv () Sequence file: RDB15118_A8B8b.seq
>15118_15118_A9Da_ME1290Rv_A01_01_ABI08.ab1     1 ATGCAATTTA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG    61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG   121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG   181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG   241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC   301 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC   361 TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC   421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG   481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG   541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG   601 TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC   661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG   721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC   781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG   841 ACCGGTGGAT CCCGGGCCCG CGGTACCGTC CACATTGCAG GGTCTTCAAG CCGAGTTCTG   901 GGAACATTTT CTGTGCTTTC TCTCATCAAG AGGTCTGAAA TCAGTGTCTC TGGGCTGGAT   961 CGTTTTCCAT ATCTCTGCCT GGTGATGAGG TTGATGTAGT GTTCGCAGCG CCGAGTAGTA  1021 TCTGGGCCAA TGGTCTTCGG CTTGGTGCGT CCTCGCCCGG GATGTCGGCC TGAAGGGCTA 1081 CGCTCAGCAG GCACCCTAGT CACACGAAGT AAGGGAACAA GGGCGA //
Primer: SV40pro_F_V2 () Sequence file: RDB15118_A8B8c.seq
>15118_15118_A8B8_SV40pro_F_V2_D01_04_ABI08.ab1     1 GACCACATTT TTTTGGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA ACAGGATGAG    61 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG   121 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT   181 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC   241 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT   301 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG   361 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG   421 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG   481 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG   541 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA   601 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA   661 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC   721 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG   781 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT   841 ATCGCCTTCT TGACGAGTTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG   901 CGACGCCCAA CCTGCCATCA CGAGATTTTC GGATTCCACC GCCGGCCTTT CTATGAAAGG   961 GTTGGGCTTT CGGAAATCGT TTTTCCGGGA ACGCCCGGCC TGGGATGAAT CCTTCCAGCG  1021 CGGGATTCTT CAATGGCTGG AAGTTCTTCG CCCCACCCCT TAGGGGGGGA GGCTAACTGG  1081 AAACACGGAG GAGACATACC GGAAGGGAAC TCGACGGCTA TGAACCGGCA ATTAAACAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl