RDB15118 - pNPY-Venus-N1

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Distribution information

Catalog number	RDB15118
Resource name	pNPY-Venus-N1
Clone info.	Venus fluorescent protein targeting localization in secretory dense-core granules of neurites. Mammalian expression. Refer Fig.2 of Nagai, T., Nat. Biotechnol. 20, 87-90, 2002.
Vector backbone	pEGFP-N1 (plasmid)
Selectable markers	Kan^r
Gene/insert name	Human NPY cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Other clones in our bank	human NPY (NCBI Gene 4852) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki

Terms and conditions set forth by the DEPOSITOR

1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Ordering

Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment

] [Bank Transfer or Check Payment

]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word

]
Approval form [Word]

by Dr. Atsushi Miyawaki

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15118	pNPY-Venus-N1	DNA solution

original	Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

Featured content	Visualization of Organelles (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

original	Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.
reference	Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345.
reference	Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140.
reference	Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

Primer: CMV_Forward Sequence file: RDB15118_A8B8a.seq
>15118_15118_A8B8_CMV-Forward_B01_02_ABI08.ab1 1 CTGTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC 121 TCAAGCTTAG CCACCATGCT AGGTAACAAG CGACTGGGGC TGTCCGGACT GACCCTCGCC 181 CTGTCCCTGC TCGTGTGCCT GGGTGCGCTG GCCGAGGCGT ACCCCTCCAA GCCGGACAAC 241 CCGGGCGAGG ACGCACCAGC GGAGGACATG GCCAGATACT ACTCGGCGCT GCGACACTAC 301 ATCAACCTCA TCACCAGGCA GAGATATGGA AAACGATCCA GCCCAGAGAC ACTGATTTCA 361 GACCTCTTGA TGAGAGAAAG CACAGAAAAT GTTCCCAGAA CTCGGCTTGA AGACCCTGCA 421 ATGTGGACGG TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG 481 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC 541 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG 601 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC 661 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG 721 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC 781 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG 841 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGG CACAAGCTGG AGTACAACTA 901 CAACAGCCAC AACGTCTATA TCACCGCCGA CAGCAGAAGA ACGGGCATCA AAGGCCCAAC 961 TTCAAGATCC GCCACAACAT CGAGGGACGG CGGCGTGCAG CTCGCCCGAC CACTACCAGC 1021 AGAACACCCC CCATCGGCGA CGGCCCCCGT TGCCTGGCTG CCCGGAACAA CCACCTAACC 1081 TTGAAGCCTT ACAAGTCCGG CCCTTGAGCA AAAGAAACCC CCAAAAACCG AGAAG //
Primer: ME1290Rv Sequence file: RDB15118_A8B8b.seq
>15118_15118_A9Da_ME1290Rv_A01_01_ABI08.ab1 1 ATGCAATTTA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG 61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG 121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG 181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG 241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC 301 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC 361 TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC 421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG 481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG 541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG 601 TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC 661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG 721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC 781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG 841 ACCGGTGGAT CCCGGGCCCG CGGTACCGTC CACATTGCAG GGTCTTCAAG CCGAGTTCTG 901 GGAACATTTT CTGTGCTTTC TCTCATCAAG AGGTCTGAAA TCAGTGTCTC TGGGCTGGAT 961 CGTTTTCCAT ATCTCTGCCT GGTGATGAGG TTGATGTAGT GTTCGCAGCG CCGAGTAGTA 1021 TCTGGGCCAA TGGTCTTCGG CTTGGTGCGT CCTCGCCCGG GATGTCGGCC TGAAGGGCTA 1081 CGCTCAGCAG GCACCCTAGT CACACGAAGT AAGGGAACAA GGGCGA //
Primer: SV40pro_F_V2 Sequence file: RDB15118_A8B8c.seq
>15118_15118_A8B8_SV40pro_F_V2_D01_04_ABI08.ab1 1 GACCACATTT TTTTGGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA ACAGGATGAG 61 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG 121 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT 181 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC 241 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT 301 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG 361 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG 421 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG 481 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG 541 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA 601 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA 661 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC 721 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG 781 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT 841 ATCGCCTTCT TGACGAGTTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 901 CGACGCCCAA CCTGCCATCA CGAGATTTTC GGATTCCACC GCCGGCCTTT CTATGAAAGG 961 GTTGGGCTTT CGGAAATCGT TTTTCCGGGA ACGCCCGGCC TGGGATGAAT CCTTCCAGCG 1021 CGGGATTCTT CAATGGCTGG AAGTTCTTCG CCCCACCCCT TAGGGGGGGA GGCTAACTGG 1081 AAACACGGAG GAGACATACC GGAAGGGAAC TCGACGGCTA TGAACCGGCA ATTAAACAAG //