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pNPY-Venus-N1

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Catalog number RDB15118
Resource name pNPY-Venus-N1
Clone info. Venus fluorescent protein targeting localization in secretory dense-core granules of neurites. Mammalian expression. Refer Fig.2 of Nagai, T., Nat. Biotechnol. 20, 87-90, 2002.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kan^r
Gene/insert name Human NPY cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human NPY Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15118 pNPY-Venus-N1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15118_A8B8p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB15118_A8B8a.seq check
          >15118_15118_A8B8_CMV-Forward_B01_02_ABI08.ab1
              1 CTGTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC
            121 TCAAGCTTAG CCACCATGCT AGGTAACAAG CGACTGGGGC TGTCCGGACT GACCCTCGCC
            181 CTGTCCCTGC TCGTGTGCCT GGGTGCGCTG GCCGAGGCGT ACCCCTCCAA GCCGGACAAC
            241 CCGGGCGAGG ACGCACCAGC GGAGGACATG GCCAGATACT ACTCGGCGCT GCGACACTAC
            301 ATCAACCTCA TCACCAGGCA GAGATATGGA AAACGATCCA GCCCAGAGAC ACTGATTTCA
            361 GACCTCTTGA TGAGAGAAAG CACAGAAAAT GTTCCCAGAA CTCGGCTTGA AGACCCTGCA
            421 ATGTGGACGG TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG
            481 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
            541 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
            601 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC
            661 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
            721 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
            781 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
            841 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGG CACAAGCTGG AGTACAACTA
            901 CAACAGCCAC AACGTCTATA TCACCGCCGA CAGCAGAAGA ACGGGCATCA AAGGCCCAAC
            961 TTCAAGATCC GCCACAACAT CGAGGGACGG CGGCGTGCAG CTCGCCCGAC CACTACCAGC
           1021 AGAACACCCC CCATCGGCGA CGGCCCCCGT TGCCTGGCTG CCCGGAACAA CCACCTAACC
          1081 TTGAAGCCTT ACAAGTCCGG CCCTTGAGCA AAAGAAACCC CCAAAAACCG AGAAG
          //
          Primer: ME1290Rv
          Sequence file: RDB15118_A8B8b.seq check
          >15118_15118_A9Da_ME1290Rv_A01_01_ABI08.ab1
              1 ATGCAATTTA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
             61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG
            121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
            181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG
            241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
            301 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC
            361 TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC
            421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
            481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
            541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
            601 TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC
            661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG
            721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC
            781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG
            841 ACCGGTGGAT CCCGGGCCCG CGGTACCGTC CACATTGCAG GGTCTTCAAG CCGAGTTCTG
            901 GGAACATTTT CTGTGCTTTC TCTCATCAAG AGGTCTGAAA TCAGTGTCTC TGGGCTGGAT
            961 CGTTTTCCAT ATCTCTGCCT GGTGATGAGG TTGATGTAGT GTTCGCAGCG CCGAGTAGTA
           1021 TCTGGGCCAA TGGTCTTCGG CTTGGTGCGT CCTCGCCCGG GATGTCGGCC TGAAGGGCTA
          1081 CGCTCAGCAG GCACCCTAGT CACACGAAGT AAGGGAACAA GGGCGA
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15118_A8B8c.seq check
          >15118_15118_A8B8_SV40pro_F_V2_D01_04_ABI08.ab1
              1 GACCACATTT TTTTGGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA ACAGGATGAG
             61 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
            121 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
            181 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
            241 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
            301 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
            361 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
            421 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
            481 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
            541 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA
            601 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
            661 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
            721 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
            781 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
            841 ATCGCCTTCT TGACGAGTTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG
            901 CGACGCCCAA CCTGCCATCA CGAGATTTTC GGATTCCACC GCCGGCCTTT CTATGAAAGG
            961 GTTGGGCTTT CGGAAATCGT TTTTCCGGGA ACGCCCGGCC TGGGATGAAT CCTTCCAGCG
           1021 CGGGATTCTT CAATGGCTGG AAGTTCTTCG CCCCACCCCT TAGGGGGGGA GGCTAACTGG
           1081 AAACACGGAG GAGACATACC GGAAGGGAAC TCGACGGCTA TGAACCGGCA ATTAAACAAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.
          reference Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345.
          reference Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140.
          reference Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl