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pNPY-Venus-N1

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Catalog number RDB15118
Resource name pNPY-Venus-N1
Clone info. Venus fluorescent protein targeting localization in secretory dense-core granules of neurites. Mammalian expression. Refer Fig.2 of Nagai, T., Nat. Biotechnol. 20, 87-90, 2002.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kan^r
Gene/insert name Human NPY cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank human NPY (NCBI Gene 4852) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
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RDB15118 pNPY-Venus-N1 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15118_A8B8p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Sequence file: RDB15118_A8B8a.seq check
>15118_15118_A8B8_CMV-Forward_B01_02_ABI08.ab1
    1 CTGTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC
  121 TCAAGCTTAG CCACCATGCT AGGTAACAAG CGACTGGGGC TGTCCGGACT GACCCTCGCC
  181 CTGTCCCTGC TCGTGTGCCT GGGTGCGCTG GCCGAGGCGT ACCCCTCCAA GCCGGACAAC
  241 CCGGGCGAGG ACGCACCAGC GGAGGACATG GCCAGATACT ACTCGGCGCT GCGACACTAC
  301 ATCAACCTCA TCACCAGGCA GAGATATGGA AAACGATCCA GCCCAGAGAC ACTGATTTCA
  361 GACCTCTTGA TGAGAGAAAG CACAGAAAAT GTTCCCAGAA CTCGGCTTGA AGACCCTGCA
  421 ATGTGGACGG TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG
  481 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  541 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  601 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC
  661 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  721 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  781 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  841 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGG CACAAGCTGG AGTACAACTA
  901 CAACAGCCAC AACGTCTATA TCACCGCCGA CAGCAGAAGA ACGGGCATCA AAGGCCCAAC
  961 TTCAAGATCC GCCACAACAT CGAGGGACGG CGGCGTGCAG CTCGCCCGAC CACTACCAGC
 1021 AGAACACCCC CCATCGGCGA CGGCCCCCGT TGCCTGGCTG CCCGGAACAA CCACCTAACC
1081 TTGAAGCCTT ACAAGTCCGG CCCTTGAGCA AAAGAAACCC CCAAAAACCG AGAAG
//
Primer: ME1290Rv
Sequence file: RDB15118_A8B8b.seq check
>15118_15118_A9Da_ME1290Rv_A01_01_ABI08.ab1
    1 ATGCAATTTA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTTACTTG
  121 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
  181 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG
  241 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
  301 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC
  361 TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC
  421 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
  481 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
  541 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
  601 TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC
  661 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG
  721 GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC
  781 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG
  841 ACCGGTGGAT CCCGGGCCCG CGGTACCGTC CACATTGCAG GGTCTTCAAG CCGAGTTCTG
  901 GGAACATTTT CTGTGCTTTC TCTCATCAAG AGGTCTGAAA TCAGTGTCTC TGGGCTGGAT
  961 CGTTTTCCAT ATCTCTGCCT GGTGATGAGG TTGATGTAGT GTTCGCAGCG CCGAGTAGTA
 1021 TCTGGGCCAA TGGTCTTCGG CTTGGTGCGT CCTCGCCCGG GATGTCGGCC TGAAGGGCTA
1081 CGCTCAGCAG GCACCCTAGT CACACGAAGT AAGGGAACAA GGGCGA
//
Primer: SV40pro_F_V2
Sequence file: RDB15118_A8B8c.seq check
>15118_15118_A8B8_SV40pro_F_V2_D01_04_ABI08.ab1
    1 GACCACATTT TTTTGGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA ACAGGATGAG
   61 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  121 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  181 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  241 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  301 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  361 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  421 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  481 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  541 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA
  601 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  661 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  721 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
  781 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
  841 ATCGCCTTCT TGACGAGTTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG
  901 CGACGCCCAA CCTGCCATCA CGAGATTTTC GGATTCCACC GCCGGCCTTT CTATGAAAGG
  961 GTTGGGCTTT CGGAAATCGT TTTTCCGGGA ACGCCCGGCC TGGGATGAAT CCTTCCAGCG
 1021 CGGGATTCTT CAATGGCTGG AAGTTCTTCG CCCCACCCCT TAGGGGGGGA GGCTAACTGG
 1081 AAACACGGAG GAGACATACC GGAAGGGAAC TCGACGGCTA TGAACCGGCA ATTAAACAAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.
reference Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345.
reference Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140.
reference Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

2020.12.17

GNP_filter3_RDBDEP_html_201216.pl