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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mKeima/pcDNA3 (#RDB15233)

Plasmid clone of monomeric Keima-Red (mKeima).

Clone info. Plasmid clone of monomeric Keima-Red (mKeima).
Vector backbone pcDNA3 (Plasmid)
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15233 mKeima/pcDNA3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mKeima/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15233).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18368_B0Gkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'
Sequence file: RDB18368_B0Gka.seq check
>D04498L1_B0Gk_1_CMV_Forward_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCC NTTGNNNCNN ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CCATGGTGAG
  181 TGTGATCGCT AAACAAATGA CCTACAAGGT TTATATGTCA GGCACGGTCA ATGGACACTA
  241 CTTTGAGGTC GAAGGCGATG GAAAAGGAAA GCCTTACGAG GGAGAGCAGA CAGTAAAGCT
  301 CACTGTCACC AAGGGTGGAC CTCTGCCATT TGCTTGGGAT ATTTTATCAC CACAGCTTCA
  361 GTACGGAAGC ATACCATTCA CCAAGTACCC TGAAGACATC CCTGATTATT TCAAGCAGTC
  421 ATTCCCTGAG GGATATACAT GGGAGAGGAG CATGAACTTT GAAGATGGTG CAGTGTGTAC
  481 TGTCAGCAAT GATTCCAGCA TCCAAGGCAA CTGTTTCATC TACAATGTCA AAATCTCTGG
  541 TGAGAACTTT CCTCCCAATG GACCTGTTAT GCAGAAGAAG ACACAGGGCT GGGAACCCAG
  601 CACTGAGCGT CTCTTTGCAC GAGATGGAAT GCTGATAGGA AACGATTATA TGGCTCTGAA
  661 GTTGGAAGGA GGTGGTCACT ATTTGTGTGA ATTTAAATCT ACTTACNAGG CAAAGAAGCC
  721 TGTGAGGATG CNNGGGCGCC ACGAGATTGA CCGCAAACTG GATGTAANCA GTCACAACAG
  781 GGATTACACA TCTGTTGAGC AGTGTGAAAT ANCCNTTGCA CGCCACTCTT TGCTCGGTTG
  841 ANAATTCTGC ANATATCCAT CACACTGGNG GCCGCTCGAN CATGNNTCTA GANGNNCCTA
  901 TTCTATAGTG NCNNCTNAAT GCTNNANCTC GCTGNNCNGC CNCNNCTGNG CCTTCTANNN
961 NNNAGNCNTC TGNNGNTTNN NNNTCCCCNN NGNNNNNNNN GANCNNNNNN NNNN
//
Primer: BGH_rev3 (Pr0885)
Region: BGH p(A), insert 3'
Sequence file: RDB18368_B0Gkb.seq check
>D04498L1_B0Gk_1_bGH_rev3_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC TCTAGATGCA
  121 TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTCAACCGAG CAAAGAGTGG
  181 CGTGCAATGG CTATTTCACA CTGCTCAACA GATGTGTAAT CCCTGTTGTG ACTGGTTACA
  241 TCCAGTTTGC GGTCAATCTC GTGGCGCCCT GGCATCCTCA CAGGCTTCTT TGCCTTGTAA
  301 GTAGATTTAA ATTCACACAA ATAGTGACCA CCTCCTTCCA ACTTCAGAGC CATATAATCG
  361 TTTCCTATCA GCATTCCATC TCGTGCAAAG AGACGCTCAG TGCTGGGTTC CCAGCCCTGT
  421 GTCTTCTTCT GCATAACAGG TCCATTGGGA GGAAAGTTCT CACCAGAGAT TTTGACATTG
  481 TAGATGAAAC AGTTGCCTTG GATGCTGGAA TCATTGCTGA CAGTACACAC TGCACCATCT
  541 TCAAAGTTCA TGCTCCTCTC CCATGTATAT CCCTCAGGGA ATGACTGCTT GAAATAATCA
  601 GGGATGTCTT CAGGGTACTT GGTGAATGGT ATGCTTCCGT ACTGAAGCTG TGGTGATAAA
  661 ATATCCCAAG CAAATGGCAG AGGTCCACCC TTGGTGACAG TGAGCTTTAC TGTCTGCTCT
  721 CCCTCGTAAG GCTTTCCTTT TCCATCGCCT TCGACCTCAA AGTAGTGTCC ATTGACCGTG
  781 CCTGACATAT AAACCTTGTA NGTCATTTGT TTAGCGATCA CACTCACCAT GGTGGATCCG
  841 ANCTCGGTAC CAAGCTTGGG TCTCCNTATA GTGAGTCGTA TTANTTTCNA TAANCCAGTA
  901 AGCAGTGGGT TCNCNAGTTA NCCAGANANC NCTGCTNATA TAGANCNCCN ACCGNANACG
  961 CCNACCGCCN ATTTNCGTCN NNGGGGCNGN ANTTGNNANN ANNTTTTNGA AAGNCCNNNN
1021 GANTTTNGNN GNNNAANNNA NNNNCNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR
Sequence file: RDB18368_B0Gkc.seq check
>D04498L1_B0Gk_1_SV40pro_ori_F_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNC GCCTCTGCCT CTGANCTNTT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
  481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
  721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NTCGACTGTG
  781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG ACNTAGCGTT GGCTANCCGN GATATTGCTG
  841 AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGNGCT TTACNGNATC GCCGCTCCCG
  901 ATTCNNAGCG CATCGCCTTC TATCGCCNTC TTGANNANTN CNTCTGANCG GGNNTCNGGG
  961 NNNCNAANNG ACCGANCNNG CGACGCCCNA NCNNGNNNTC NCNANNTTTC NNNTNNNNCN
1021 NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]