Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mKeima/pcDNA3

Plasmid clone of monomeric Keima-Red (mKeima).

Catalog number RDB15233
Resource name mKeima/pcDNA3
Clone info. Plasmid clone of monomeric Keima-Red (mKeima).
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15233 mKeima/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18368_B0Gkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB18368_B0Gka.seq check
>D04498L1_B0Gk_1_CMV_Forward_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCC NTTGNNNCNN ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CCATGGTGAG
  181 TGTGATCGCT AAACAAATGA CCTACAAGGT TTATATGTCA GGCACGGTCA ATGGACACTA
  241 CTTTGAGGTC GAAGGCGATG GAAAAGGAAA GCCTTACGAG GGAGAGCAGA CAGTAAAGCT
  301 CACTGTCACC AAGGGTGGAC CTCTGCCATT TGCTTGGGAT ATTTTATCAC CACAGCTTCA
  361 GTACGGAAGC ATACCATTCA CCAAGTACCC TGAAGACATC CCTGATTATT TCAAGCAGTC
  421 ATTCCCTGAG GGATATACAT GGGAGAGGAG CATGAACTTT GAAGATGGTG CAGTGTGTAC
  481 TGTCAGCAAT GATTCCAGCA TCCAAGGCAA CTGTTTCATC TACAATGTCA AAATCTCTGG
  541 TGAGAACTTT CCTCCCAATG GACCTGTTAT GCAGAAGAAG ACACAGGGCT GGGAACCCAG
  601 CACTGAGCGT CTCTTTGCAC GAGATGGAAT GCTGATAGGA AACGATTATA TGGCTCTGAA
  661 GTTGGAAGGA GGTGGTCACT ATTTGTGTGA ATTTAAATCT ACTTACNAGG CAAAGAAGCC
  721 TGTGAGGATG CNNGGGCGCC ACGAGATTGA CCGCAAACTG GATGTAANCA GTCACAACAG
  781 GGATTACACA TCTGTTGAGC AGTGTGAAAT ANCCNTTGCA CGCCACTCTT TGCTCGGTTG
  841 ANAATTCTGC ANATATCCAT CACACTGGNG GCCGCTCGAN CATGNNTCTA GANGNNCCTA
  901 TTCTATAGTG NCNNCTNAAT GCTNNANCTC GCTGNNCNGC CNCNNCTGNG CCTTCTANNN
961 NNNAGNCNTC TGNNGNTTNN NNNTCCCCNN NGNNNNNNNN GANCNNNNNN NNNN
//
Primer: BGH_rev3
Region: BGH p(A), insert 3'
Sequence file: RDB18368_B0Gkb.seq check
>D04498L1_B0Gk_1_bGH_rev3_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC TCTAGATGCA
  121 TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTCAACCGAG CAAAGAGTGG
  181 CGTGCAATGG CTATTTCACA CTGCTCAACA GATGTGTAAT CCCTGTTGTG ACTGGTTACA
  241 TCCAGTTTGC GGTCAATCTC GTGGCGCCCT GGCATCCTCA CAGGCTTCTT TGCCTTGTAA
  301 GTAGATTTAA ATTCACACAA ATAGTGACCA CCTCCTTCCA ACTTCAGAGC CATATAATCG
  361 TTTCCTATCA GCATTCCATC TCGTGCAAAG AGACGCTCAG TGCTGGGTTC CCAGCCCTGT
  421 GTCTTCTTCT GCATAACAGG TCCATTGGGA GGAAAGTTCT CACCAGAGAT TTTGACATTG
  481 TAGATGAAAC AGTTGCCTTG GATGCTGGAA TCATTGCTGA CAGTACACAC TGCACCATCT
  541 TCAAAGTTCA TGCTCCTCTC CCATGTATAT CCCTCAGGGA ATGACTGCTT GAAATAATCA
  601 GGGATGTCTT CAGGGTACTT GGTGAATGGT ATGCTTCCGT ACTGAAGCTG TGGTGATAAA
  661 ATATCCCAAG CAAATGGCAG AGGTCCACCC TTGGTGACAG TGAGCTTTAC TGTCTGCTCT
  721 CCCTCGTAAG GCTTTCCTTT TCCATCGCCT TCGACCTCAA AGTAGTGTCC ATTGACCGTG
  781 CCTGACATAT AAACCTTGTA NGTCATTTGT TTAGCGATCA CACTCACCAT GGTGGATCCG
  841 ANCTCGGTAC CAAGCTTGGG TCTCCNTATA GTGAGTCGTA TTANTTTCNA TAANCCAGTA
  901 AGCAGTGGGT TCNCNAGTTA NCCAGANANC NCTGCTNATA TAGANCNCCN ACCGNANACG
  961 CCNACCGCCN ATTTNCGTCN NNGGGGCNGN ANTTGNNANN ANNTTTTNGA AAGNCCNNNN
1021 GANTTTNGNN GNNNAANNNA NNNNCNNNNN NNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR
Sequence file: RDB18368_B0Gkc.seq check
>D04498L1_B0Gk_1_SV40pro_ori_F_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNC GCCTCTGCCT CTGANCTNTT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
  481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
  721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NTCGACTGTG
  781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG ACNTAGCGTT GGCTANCCGN GATATTGCTG
  841 AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGNGCT TTACNGNATC GCCGCTCCCG
  901 ATTCNNAGCG CATCGCCTTC TATCGCCNTC TTGANNANTN CNTCTGANCG GGNNTCNGGG
  961 NNNCNAANNG ACCGANCNNG CGACGCCCNA NCNNGNNNTC NCNANNTTTC NNNTNNNNCN
1021 NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.08.13

GNP_filter3_RDBDEP_html_210618.pl