Please review the QC test results indicated by icon below as well as clone information before placing your order.

GEPRA-AA

Genetically encoded indicator for RA, GEPRA. Low-affinity verson of the GEPRA-B.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15251_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F (Pr0506) Region: CMV IE94 pro, SP6 pro, insert 5' Sequence file: RDB15251_A7H9a.seq
>D04498F9_1_A7H9_pCMV-IE94_F8_F05_17_ABI24.ab1     1 AGGGGGCGGG TGGGCGGTGC CTAATGGGAG GTCTATATAA GCAATGCTCG TTTAGGGAAC    61 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG   121 CTACTTGTTC TTTTTGCAGG ATCCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT   181 GGTGCCCATC CTGGTCGAGC TGCAGGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG   241 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG   301 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT   361 CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGTCA TGCCCGAAGG   421 CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA   481 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA   541 GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACATCAGCC ACAACGTCTA   601 TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC GCCACAACAT   661 CGTGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG   721 CCCCGTGAAC CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC   781 CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCC GCATGCATGA   841 GAGCTATGAG ATGACAGCGG AGCTAGACGA CCTCACTGAG AAAGATCCGG AAAGCCCACC   901 AGGAAACCTT TCCCTCACTC TGCCAGCTGG GTAAATACAC CACGAATTCC AGCGCTGACC   961 ACCGGGTCCG ATTGGACTTG GGGCCCTCTG GGGACAAATT CAGTGAGCTG GCCCACCAAG  1021 TGCATTATTA AGATCGTGGA GTTTCGCCAA GCGTCTGCCC GGGCTTCACA GGTCTGACAT  1081 CGCAGACCCA GAATCACCCT TGCTTCAAGG CGACCTGCCT TGGAAATATC TTTGGAATTT 1141 CCTCTCAGGA //
Primer: pAxCALNLF1 (Pr0166) Region: SV40 p(A), T7 pro, insert 3' Sequence file: RDB15251_A7H9b.seq
>D04498F9-1_A7H9_pAxCALNL_F1_B10_04_ABI24.ab1     1 CCAATATATC TTAATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT    61 CGAGTTACTT GTACAGCTCG TTCATGCCTT CAGTGATCCC GGCGGCGGTC AGGAACTCCA   121 GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGAA CAGGGCGGAC TGGTAGCTCA   181 GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT   241 GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTGGCCTTGA   301 TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA   361 GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT   421 TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT   481 TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT   541 GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC AGGGTGGTCA   601 CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GAGCAGCTTC AGGGTCAGCT   661 TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT   721 CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA   781 CCATGAGCTC TTCATGTCCT TCAGAATTCT CCAGCATTTC CTGAATGAGA GGTGGCATTG   841 ATCCAGGAAT TTCCATTTTC AAGGTAATTA CACGTTCGGC ACCTTTCGCG CTGATGCTGC   901 GGAGATCTGT GATTTTCATT TAGATCTTTG GAAACATGTG AGGCTTGCTG GGTCGTCGTT   961 TTCTAATGTA ATCTTTAGTG CTTCAGCAGT GATTCTTGAA GCTTGTCTAC TTTGTGGTCT  1021 CAGTCTTGCC GGTCTCCACA GATAAACAGA TGCACTTGGA CAGGCTGTTC TTGGTTCATC 1081 ATCGAACCAG ACTGCTGCAA AGGTGACACT CGA //
Primer: RARbeta_LBD (Pr0507) Region: insert mid Sequence file: RDB15251_A7H9c.seq
>D04498F9_1_A7H9_RARbeta_LBD_G05_20_ABI24.ab1     1 CGGTAGGGAG AGGGTGGCAT TGATCCAGGA ATTTCCATTT TCAAGGTAAT TACACGTTCG    61 GCACCTTTCG CGCTGATGCT GCGGAGATCT GTGATTTTCA TTAAGATCTT TGGAAACATG   121 TGAGGCTTGC TGGGTCGTCG TTTTCTAATG TAAATCTTTA GTGCTTCCAG CAGTGGTTCT   181 TGGAGCTTGT CTACTTTTGT TGGTTCCTCA AGGTCCTGGC GGTCTCCACA GATTAAACAG   241 ATGGCACTGA GAAGGCCTGT TTCTGTGTCA TCCATTTCCA AAGGCAGGAG CTGGTTGGCA   301 AAGGTGAACA CAAGGTCAGT CAGAGGACCG AAGCCAGCAT TGTGCATCTG AGTTCGATTT   361 AGTGTAAGGC CATCAGCGAA AGTCATGGTG TCTTGCTCTG GGGTATAGGC GGTACAAATT   421 CTGAGAATCA AGATATCCAA GCAGGCGGCT TTGAGCAGGG TGATCTGGTC TGCGATGGTC   481 AGACCTGTGA AGCCCGGCAG ACGCTTGGCG AACTCCACGA TCTTAATAAT GCACTTGGTG   541 GCCAGCTCAC TGAATTTGTC CCAGAGGCCC AAGTCCAATC GGACCCGGTG GTCAGCGCTG   601 GAATTCGTGG TGTATTTACC CAGCTGGCAG AGTGAGGGAA AGGTTTCCTG GTGGGCTTTC   661 CGGATCTTCT CAGTGAGGTC GTCTAGCTCC GCTGTCATCT CATAGCTCTC ATGCATGCGG   721 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC   781 AGGGCGGACT GGGTGCTCAG GTAGTGGTTG TCGGGCAGGT TCACGGGGCC GTCGCCGATG   841 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCACGAT GTTGTGGCGG   901 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG   961 CTGATGTAGT TGTACTCCCA GCTTGTGCCC CAGGATGTTG CCCGTCCTCC TTGAAGTCGA  1021 TGCCCTTTCA GCTCGATGCG GTTCACAAGG TGTCGCCCTC GAACTCACTC GCGCGGGTCT  1081 TGGTAAGTGG CCGTTCGTCT TTGGAGAGAT GTGCGCTCCT GACGTAGCCT TTCAGGACCA 1141 TTGAAACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl