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GEPRA-AA

Genetically encoded indicator for RA, GEPRA. Low-affinity verson of the GEPRA-B.

Catalog number RDB15251
Resource name GEPRA-AA
Clone info. Genetically encoded indicator for RA, GEPRA. Low-affinity verson of the GEPRA-B, having ligand binding domain from the RAR-beta with two amino acid substitutions (R269A and S280A).
Vector backbone pCS2+ (plasmid)
Gene/insert name mouse RARbeta cDNA Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |
 
Other clones in our bank mouse Rarb (NCBI Gene 218772) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15251 GEPRA-AA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Sensor and Visualization (English text)

References

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15251_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F RDB15251_A7H9a.seq
>D04498F9_1_A7H9_pCMV-IE94_F8_F05_17_ABI24.ab1
    1 AGGGGGCGGG TGGGCGGTGC CTAATGGGAG GTCTATATAA GCAATGCTCG TTTAGGGAAC
   61 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
  121 CTACTTGTTC TTTTTGCAGG ATCCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT
  181 GGTGCCCATC CTGGTCGAGC TGCAGGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG
  241 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG
  301 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT
  361 CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGTCA TGCCCGAAGG
  421 CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA
  481 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA
  541 GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACATCAGCC ACAACGTCTA
  601 TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC GCCACAACAT
  661 CGTGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG
  721 CCCCGTGAAC CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC
  781 CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCC GCATGCATGA
  841 GAGCTATGAG ATGACAGCGG AGCTAGACGA CCTCACTGAG AAAGATCCGG AAAGCCCACC
  901 AGGAAACCTT TCCCTCACTC TGCCAGCTGG GTAAATACAC CACGAATTCC AGCGCTGACC
  961 ACCGGGTCCG ATTGGACTTG GGGCCCTCTG GGGACAAATT CAGTGAGCTG GCCCACCAAG
 1021 TGCATTATTA AGATCGTGGA GTTTCGCCAA GCGTCTGCCC GGGCTTCACA GGTCTGACAT
 1081 CGCAGACCCA GAATCACCCT TGCTTCAAGG CGACCTGCCT TGGAAATATC TTTGGAATTT
1141 CCTCTCAGGA
//
Primer: pAxCALNL_F1 RDB15251_A7H9b.seq
>D04498F9-1_A7H9_pAxCALNL_F1_B10_04_ABI24.ab1
    1 CCAATATATC TTAATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT
   61 CGAGTTACTT GTACAGCTCG TTCATGCCTT CAGTGATCCC GGCGGCGGTC AGGAACTCCA
  121 GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGAA CAGGGCGGAC TGGTAGCTCA
  181 GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT
  241 GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTGGCCTTGA
  301 TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA
  361 GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT
  421 TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT
  481 TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT
  541 GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC AGGGTGGTCA
  601 CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GAGCAGCTTC AGGGTCAGCT
  661 TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT
  721 CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA
  781 CCATGAGCTC TTCATGTCCT TCAGAATTCT CCAGCATTTC CTGAATGAGA GGTGGCATTG
  841 ATCCAGGAAT TTCCATTTTC AAGGTAATTA CACGTTCGGC ACCTTTCGCG CTGATGCTGC
  901 GGAGATCTGT GATTTTCATT TAGATCTTTG GAAACATGTG AGGCTTGCTG GGTCGTCGTT
  961 TTCTAATGTA ATCTTTAGTG CTTCAGCAGT GATTCTTGAA GCTTGTCTAC TTTGTGGTCT
 1021 CAGTCTTGCC GGTCTCCACA GATAAACAGA TGCACTTGGA CAGGCTGTTC TTGGTTCATC
1081 ATCGAACCAG ACTGCTGCAA AGGTGACACT CGA
//
Primer: RARbeta_LBD RDB15251_A7H9c.seq
>D04498F9_1_A7H9_RARbeta_LBD_G05_20_ABI24.ab1
    1 CGGTAGGGAG AGGGTGGCAT TGATCCAGGA ATTTCCATTT TCAAGGTAAT TACACGTTCG
   61 GCACCTTTCG CGCTGATGCT GCGGAGATCT GTGATTTTCA TTAAGATCTT TGGAAACATG
  121 TGAGGCTTGC TGGGTCGTCG TTTTCTAATG TAAATCTTTA GTGCTTCCAG CAGTGGTTCT
  181 TGGAGCTTGT CTACTTTTGT TGGTTCCTCA AGGTCCTGGC GGTCTCCACA GATTAAACAG
  241 ATGGCACTGA GAAGGCCTGT TTCTGTGTCA TCCATTTCCA AAGGCAGGAG CTGGTTGGCA
  301 AAGGTGAACA CAAGGTCAGT CAGAGGACCG AAGCCAGCAT TGTGCATCTG AGTTCGATTT
  361 AGTGTAAGGC CATCAGCGAA AGTCATGGTG TCTTGCTCTG GGGTATAGGC GGTACAAATT
  421 CTGAGAATCA AGATATCCAA GCAGGCGGCT TTGAGCAGGG TGATCTGGTC TGCGATGGTC
  481 AGACCTGTGA AGCCCGGCAG ACGCTTGGCG AACTCCACGA TCTTAATAAT GCACTTGGTG
  541 GCCAGCTCAC TGAATTTGTC CCAGAGGCCC AAGTCCAATC GGACCCGGTG GTCAGCGCTG
  601 GAATTCGTGG TGTATTTACC CAGCTGGCAG AGTGAGGGAA AGGTTTCCTG GTGGGCTTTC
  661 CGGATCTTCT CAGTGAGGTC GTCTAGCTCC GCTGTCATCT CATAGCTCTC ATGCATGCGG
  721 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC
  781 AGGGCGGACT GGGTGCTCAG GTAGTGGTTG TCGGGCAGGT TCACGGGGCC GTCGCCGATG
  841 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCACGAT GTTGTGGCGG
  901 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG
  961 CTGATGTAGT TGTACTCCCA GCTTGTGCCC CAGGATGTTG CCCGTCCTCC TTGAAGTCGA
 1021 TGCCCTTTCA GCTCGATGCG GTTCACAAGG TGTCGCCCTC GAACTCACTC GCGCGGGTCT
 1081 TGGTAAGTGG CCGTTCGTCT TTGGAGAGAT GTGCGCTCCT GACGTAGCCT TTCAGGACCA
1141 TTGAAACG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.
reference Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl