Sequencing and PCR primers for primer information.
Plasmid clone of cjBlue., a blue color protein of sea anemone Cnidopus japonicus.
| Catalog number | RDB15242 |
|---|---|
| Resource name | cjBlue/pRSETB |
| Clone info. | Plasmid clone of blue color protein cjBlue. Bacterial expression. |
| Vector backbone | pRSETB (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Cnidopus japonicus blue color protein cDNA |
| Reference of nucleotide sequence | BD408947 (DDBJ accession number) |
| Depositor | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word] |
| Remarks | Please obtain written approval of the depositor. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15242 | cjBlue/pRSETB | DNA solution |
Electronic file
Featured content
| Featured content | Dr. Miyawaki's resources (English text) |
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| Featured content | Fluorescent protein resources (English text) |
| Featured content | Fluorescent protein resources (Japanese text) |
References
| original | Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15242_A7H9p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB15242_A7H9a.seq |
|---|---|
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>D04498E9-1_A7H9_T7_20bp_F07_16_ABI24.ab1 1 GAATTCGCTG GATAATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT 61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG 121 TACGACGATG ACGATAAGGA TCCGACCATG GCTTCCAAAA TCAGCGACAA TGTACGTATC 181 AAGTTATATA TGGAGGGCAC AGTCAACAAT CATCACTTCA TGTGCGAAGC TGAAGGAGAG 241 GGCAAGCCAT ACGAGGGAAC TCAAATGGAG AACATAAAAG TCACCAAAGG AGGCCCTCTG 301 CCGTTCTCTT TTGATATCTT GACGCCTAAC TGCCAATATG GAAGCGTAGC CATAACCAAG 361 TATACATCAG GGATTCCAGA CTACTTTAAG CAATCTTTTC CTGAAGGATT TACCTGGGAA 421 AGAACCACAA TCTACGAAGA TGGGGCTTAC CTTACAACTC AACAAGAAAC CAAACTTGAT 481 GGAAATTGCC TCGTCTACAA TATTAAAATC CTTGGATGTA ATTTTCCCCC CAATGGTCCT 541 GTGATGCAGA AGAAAACCCA AGGCTGGGAA CCCTGTTGCG AGATGCGCTA TACACGTGAT 601 GGTGTGCTAT GTGGCCAAAC ATTAATGGCA CTTAAATGCG CCGATGGGAA CCACCTCACT 661 TGCCATCTGA GAACTACTTA CAGGTCCAAA AAGGCAGCAA AGGCGTTGCA GATGCCACCC 721 TTCCATTTTT CAGACCATCG TCCTGAAATA GTGAAGGTTT CAGAGAACGG CACACTATTT 781 GAACAGCACG AAAGTTCAGT GGCCAGGTAC TGTCAAACAT GCCCATCTAA ACTTGGTCAC 841 AATTAAAGGA CAACACTAGC TTCATTCCAA CCAGGACAAA CTTAGTTTCA TGGATATTTT 901 TAATAGTTTT TCCCTATCAG TGTCCGATTC CTAATTCCAA TTCCTAACTA GATATTTCAT 961 TTGAATTGTC ATTGATTTAT ATGGCTGTAA CATTGTGCAT GCAACAATTT GTTTTCTGAG 1021 ATTGATTGTG TCTTTGAAAT CAATATAACT CATACCTTTT AAATTTAAAA AAGAAAGACG 1081 AGAGAAAAAG AAAACTAATT TCCCTGCAGT CTCGCGAATC TCCGAAGGGC ATCGTA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. |
|---|---|
| reference | Li, Y., Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging. Sci. Rep. 6: 22129 (2016). PMID 26926390. |
| reference | Laufer, J., In vitro characterization of genetically expressed absorbing proteins using photoacoustic spectroscopy. Biomed. Opt. Express 4 (11): 2477-2490 (2013). PMID 24298408. |
| reference | Min, W., Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature 461 (7267): 1105-1109 (2009). PMID 19847261. |
2019.03.08
GNP_filter3_RDBDEP_html_190307.pl