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cjBlue/pRSETB

Plasmid clone of cjBlue., a blue color protein of sea anemone Cnidopus japonicus.

Catalog number RDB15242
Resource name cjBlue/pRSETB
Clone info. Plasmid clone of blue color protein cjBlue. Bacterial expression.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Cnidopus japonicus blue color protein cDNA
Reference of insert sequence BD408947 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Cnidopus japonicus blue color protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
            5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15242 cjBlue/pRSETB DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15242_A7H9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp
            Sequence file: RDB15242_A7H9a.seq check
            >D04498E9-1_A7H9_T7_20bp_F07_16_ABI24.ab1
                1 GAATTCGCTG GATAATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
               61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
              121 TACGACGATG ACGATAAGGA TCCGACCATG GCTTCCAAAA TCAGCGACAA TGTACGTATC
              181 AAGTTATATA TGGAGGGCAC AGTCAACAAT CATCACTTCA TGTGCGAAGC TGAAGGAGAG
              241 GGCAAGCCAT ACGAGGGAAC TCAAATGGAG AACATAAAAG TCACCAAAGG AGGCCCTCTG
              301 CCGTTCTCTT TTGATATCTT GACGCCTAAC TGCCAATATG GAAGCGTAGC CATAACCAAG
              361 TATACATCAG GGATTCCAGA CTACTTTAAG CAATCTTTTC CTGAAGGATT TACCTGGGAA
              421 AGAACCACAA TCTACGAAGA TGGGGCTTAC CTTACAACTC AACAAGAAAC CAAACTTGAT
              481 GGAAATTGCC TCGTCTACAA TATTAAAATC CTTGGATGTA ATTTTCCCCC CAATGGTCCT
              541 GTGATGCAGA AGAAAACCCA AGGCTGGGAA CCCTGTTGCG AGATGCGCTA TACACGTGAT
              601 GGTGTGCTAT GTGGCCAAAC ATTAATGGCA CTTAAATGCG CCGATGGGAA CCACCTCACT
              661 TGCCATCTGA GAACTACTTA CAGGTCCAAA AAGGCAGCAA AGGCGTTGCA GATGCCACCC
              721 TTCCATTTTT CAGACCATCG TCCTGAAATA GTGAAGGTTT CAGAGAACGG CACACTATTT
              781 GAACAGCACG AAAGTTCAGT GGCCAGGTAC TGTCAAACAT GCCCATCTAA ACTTGGTCAC
              841 AATTAAAGGA CAACACTAGC TTCATTCCAA CCAGGACAAA CTTAGTTTCA TGGATATTTT
              901 TAATAGTTTT TCCCTATCAG TGTCCGATTC CTAATTCCAA TTCCTAACTA GATATTTCAT
              961 TTGAATTGTC ATTGATTTAT ATGGCTGTAA CATTGTGCAT GCAACAATTT GTTTTCTGAG
             1021 ATTGATTGTG TCTTTGAAAT CAATATAACT CATACCTTTT AAATTTAAAA AAGAAAGACG
            1081 AGAGAAAAAG AAAACTAATT TCCCTGCAGT CTCGCGAATC TCCGAAGGGC ATCGTA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.
            reference Li, Y., Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging. Sci. Rep. 6: 22129 (2016). PMID 26926390.
            reference Laufer, J., In vitro characterization of genetically expressed absorbing proteins using photoacoustic spectroscopy. Biomed. Opt. Express 4 (11): 2477-2490 (2013). PMID 24298408.
            reference Min, W., Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature 461 (7267): 1105-1109 (2009). PMID 19847261.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl