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cjBlue/pRSETB

Plasmid clone of cjBlue., a blue color protein of sea anemone Cnidopus japonicus.

Catalog number RDB15242
Resource name cjBlue/pRSETB
Clone info. Plasmid clone of blue color protein cjBlue. Bacterial expression.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Cnidopus japonicus blue color protein cDNA
Reference of nucleotide sequence BD408947 (DDBJ accession number)
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Prive, G.G., Miyawaki, A., Ikura, M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
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Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15242 cjBlue/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15242_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15242_A7H9a.seq
>D04498E9-1_A7H9_T7_20bp_F07_16_ABI24.ab1
    1 GAATTCGCTG GATAATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
   61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
  121 TACGACGATG ACGATAAGGA TCCGACCATG GCTTCCAAAA TCAGCGACAA TGTACGTATC
  181 AAGTTATATA TGGAGGGCAC AGTCAACAAT CATCACTTCA TGTGCGAAGC TGAAGGAGAG
  241 GGCAAGCCAT ACGAGGGAAC TCAAATGGAG AACATAAAAG TCACCAAAGG AGGCCCTCTG
  301 CCGTTCTCTT TTGATATCTT GACGCCTAAC TGCCAATATG GAAGCGTAGC CATAACCAAG
  361 TATACATCAG GGATTCCAGA CTACTTTAAG CAATCTTTTC CTGAAGGATT TACCTGGGAA
  421 AGAACCACAA TCTACGAAGA TGGGGCTTAC CTTACAACTC AACAAGAAAC CAAACTTGAT
  481 GGAAATTGCC TCGTCTACAA TATTAAAATC CTTGGATGTA ATTTTCCCCC CAATGGTCCT
  541 GTGATGCAGA AGAAAACCCA AGGCTGGGAA CCCTGTTGCG AGATGCGCTA TACACGTGAT
  601 GGTGTGCTAT GTGGCCAAAC ATTAATGGCA CTTAAATGCG CCGATGGGAA CCACCTCACT
  661 TGCCATCTGA GAACTACTTA CAGGTCCAAA AAGGCAGCAA AGGCGTTGCA GATGCCACCC
  721 TTCCATTTTT CAGACCATCG TCCTGAAATA GTGAAGGTTT CAGAGAACGG CACACTATTT
  781 GAACAGCACG AAAGTTCAGT GGCCAGGTAC TGTCAAACAT GCCCATCTAA ACTTGGTCAC
  841 AATTAAAGGA CAACACTAGC TTCATTCCAA CCAGGACAAA CTTAGTTTCA TGGATATTTT
  901 TAATAGTTTT TCCCTATCAG TGTCCGATTC CTAATTCCAA TTCCTAACTA GATATTTCAT
  961 TTGAATTGTC ATTGATTTAT ATGGCTGTAA CATTGTGCAT GCAACAATTT GTTTTCTGAG
 1021 ATTGATTGTG TCTTTGAAAT CAATATAACT CATACCTTTT AAATTTAAAA AAGAAAGACG
1081 AGAGAAAAAG AAAACTAATT TCCCTGCAGT CTCGCGAATC TCCGAAGGGC ATCGTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187.
reference Li, Y., Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging. Sci. Rep. 6: 22129 (2016). PMID 26926390.
reference Laufer, J., In vitro characterization of genetically expressed absorbing proteins using photoacoustic spectroscopy. Biomed. Opt. Express 4 (11): 2477-2490 (2013). PMID 24298408.
reference Min, W., Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature 461 (7267): 1105-1109 (2009). PMID 19847261.

2019.03.08

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