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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

cjBlue/pRSETB (#RDB15242)

Plasmid clone of cjBlue., a blue color protein of sea anemone Cnidopus japonicus.

Clone info. Plasmid clone of blue color protein cjBlue. Bacterial expression.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC.
Gene/insert name Cnidopus japonicus blue color protein cDNA
Reference of insert sequence BD408947 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15242 cjBlue/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The cjBlue/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15242).

Reference section:

Chan, M.C., Karasawa, S., Mizuno, H., Bosanac, I., Ho, D., Privé, G.G., Miyawaki, A., Ikura, M., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15242_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp ()
Sequence file: RDB15242_A7H9a.seq check
>D04498E9-1_A7H9_T7_20bp_F07_16_ABI24.ab1
    1 GAATTCGCTG GATAATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
   61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
  121 TACGACGATG ACGATAAGGA TCCGACCATG GCTTCCAAAA TCAGCGACAA TGTACGTATC
  181 AAGTTATATA TGGAGGGCAC AGTCAACAAT CATCACTTCA TGTGCGAAGC TGAAGGAGAG
  241 GGCAAGCCAT ACGAGGGAAC TCAAATGGAG AACATAAAAG TCACCAAAGG AGGCCCTCTG
  301 CCGTTCTCTT TTGATATCTT GACGCCTAAC TGCCAATATG GAAGCGTAGC CATAACCAAG
  361 TATACATCAG GGATTCCAGA CTACTTTAAG CAATCTTTTC CTGAAGGATT TACCTGGGAA
  421 AGAACCACAA TCTACGAAGA TGGGGCTTAC CTTACAACTC AACAAGAAAC CAAACTTGAT
  481 GGAAATTGCC TCGTCTACAA TATTAAAATC CTTGGATGTA ATTTTCCCCC CAATGGTCCT
  541 GTGATGCAGA AGAAAACCCA AGGCTGGGAA CCCTGTTGCG AGATGCGCTA TACACGTGAT
  601 GGTGTGCTAT GTGGCCAAAC ATTAATGGCA CTTAAATGCG CCGATGGGAA CCACCTCACT
  661 TGCCATCTGA GAACTACTTA CAGGTCCAAA AAGGCAGCAA AGGCGTTGCA GATGCCACCC
  721 TTCCATTTTT CAGACCATCG TCCTGAAATA GTGAAGGTTT CAGAGAACGG CACACTATTT
  781 GAACAGCACG AAAGTTCAGT GGCCAGGTAC TGTCAAACAT GCCCATCTAA ACTTGGTCAC
  841 AATTAAAGGA CAACACTAGC TTCATTCCAA CCAGGACAAA CTTAGTTTCA TGGATATTTT
  901 TAATAGTTTT TCCCTATCAG TGTCCGATTC CTAATTCCAA TTCCTAACTA GATATTTCAT
  961 TTGAATTGTC ATTGATTTAT ATGGCTGTAA CATTGTGCAT GCAACAATTT GTTTTCTGAG
 1021 ATTGATTGTG TCTTTGAAAT CAATATAACT CATACCTTTT AAATTTAAAA AAGAAAGACG
1081 AGAGAAAAAG AAAACTAATT TCCCTGCAGT CTCGCGAATC TCCGAAGGGC ATCGTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Chan, M.C., Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281 (49): 37813-37819 (2006). PMID 17028187. [link to RRC of NBRP]
reference Li, Y., Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging. Sci. Rep. 6: 22129 (2016). PMID 26926390.
reference Laufer, J., In vitro characterization of genetically expressed absorbing proteins using photoacoustic spectroscopy. Biomed. Opt. Express 4 (11): 2477-2490 (2013). PMID 24298408.
reference Min, W., Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature 461 (7267): 1105-1109 (2009). PMID 19847261.