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hmKO2/pRSETB

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.

Catalog number RDB15313
Resource name hmKO2/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15313 hmKO2/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15313_A7Htp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15313_A7Hta.seq
>D04498J4_A7H9_1_T7_20bp_E08_14_ABI24.ab1
    1 GAAAATCAGT AGAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT
   61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT
  121 CTGTACGACG ATGACGATAA GGATCCGACC ATGGTGAGCG TGATCAAGCC CGAGATGAAG
  181 ATGAGGTACT ACATGGACGG CTCCGTCAAT GGGCATGAGT TCACAATCGA GGGTGAGGGC
  241 ACAGGCAGAC CTTACGAGGG ACATCAGGAG ATGACACTGC GCGTCACAAT GGCCGAGGGC
  301 GGGCCAATGC CTTTCGCCTT CGACCTGGTG TCCCACGTGT TCTGTTACGG CCACAGAGTT
  361 TTTACCAAGT ACCCAGAAGA GATCCCAGAC TATTTCAAGC AGGCCTTTCC TGAGGGCCTG
  421 TCCTGGGAGA GGTCCCTGGA GTTCGAGGAC GGCGGCTCCG CCTCCGTGAG CGCCCACATC
  481 AGCCTGAGGG GCAACACCTT CTACCACAAG TCCAAGTTCA CCGGCGTGAA CTTCCCCGCC
  541 GACGGCCCCA TCATGCAGAA CCAGAGCGTG GACTGGGAGC CCTCCACCGA GAAGATCACC
  601 GCCAGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGA GGGCGGCGGC
  661 AACCACAAGT GCCAGATGAA GACCACCTAC AAGGCCGCCA AGGAGATCCT GGAGATGCCC
  721 GGCGACCACT ACATCGGCCA CAGGCTGGTG AGGAAGACCG AGGGCAACAT CACCGAGCAG
  781 GTGGAGGACG CCGTGGCCCA CTCCTAAGAA TTCGAAGCTT GATCCGGCTG CTAACAAAGC
  841 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
  901 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATCTGG
  961 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTTGCGCA GCCCTGAAAT
 1021 GGGCGAAATG GGACGCGCCC CTGTAGCGGC GCATTAGGCC GCCGGCCGGT TGTGGTGGGT
1081 TACGCCGCAG CGTTGAACGC TTACACTTGC CAGCCGTCCC TTTAGACGAT CTTCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

2019.08.08

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