RDB15313 - hmKO2/pRSETB

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

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QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15313	hmKO2/pRSETB	DNA solution

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: T7_20bp (Pr0261) Region: 6xHis,T7 Tag,Xpress tag,insert 5' Sequence file: RDB15313_A7H9b.seq
>D04498J4_A7H9_1_T7_20bp_E08_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTTT TGTTNNNCTN NNNNAAGGAG NNATACATAT GCGGGGTTCT 61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT 121 CTGTACGACG ATGACGATAA GGATCCGACC ATGGTGAGCG TGATCAAGCC CGAGATGAAG 181 ATGAGGTACT ACATGGACGG CTCCGTCAAT GGGCATGAGT TCACAATCGA GGGTGAGGGC 241 ACAGGCAGAC CTTACGAGGG ACATCAGGAG ATGACACTGC GCGTCACAAT GGCCGAGGGC 301 GGGCCAATGC CTTTCGCCTT CGACCTGGTG TCCCACGTGT TCTGTTACGG CCACAGAGTT 361 TTTACCAAGT ACCCAGAAGA GATCCCAGAC TATTTCAAGC AGGCCTTTCC TGAGGGCCTG 421 TCCTGGGAGA GGTCCCTGGA GTTCGAGGAC GGCGGCTCCG CCTCCGTGAG CGCCCACATC 481 AGCCTGAGGG GCAACACCTT CTACCACAAG TCCAAGTTCA CCGGCGTGAA CTTCCCCGCC 541 GACGGCCCCA TCATGCAGAA CCAGAGCGTG GACTGGGAGC CCTCCACCGA GAAGATCACC 601 GCCAGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGA GGGCGGCGGC 661 AACCACAAGT GCCAGATGAA GACCACCTAC AAGGCCGCCA AGGAGATCCT GGAGATGCCC 721 GGCGANCACT ACATCGGCCA CAGGCTGGTG ANGAAGANCG AGGGCAACAT CACCGANCAN 781 GTGGANGACG CCGTGGCCCA CTCCTAAGAN TTCGAAGCTT GNTCCGGCTG CTNACAAAGC 841 CCGAAAGGAA GCTGANTTGG CTGCTGCCAC CGCNNANCAA TAACTAGCAT AACCCCTTGG 901 GGCCTCTNAA CGGGNCTNGA GGGGTTTTTN GCTGAAAGGA NGNNNTATAT CCGGNNNNGG 961 NGTAANANCN NNNNNGNCNN CANNNNTNNN CNNNNCNNNN NNNNNN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter,insert 3' Sequence file: RDB15313_A7H9c.seq
>D04498J4_A7H9_1_f1ori_R3_F08_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNANNNTTGG GNNNGGCGNN CGGTGCGGGC CTCTTCGCTA 61 TTACGCCNGA TCCGGATATA GTTCCTCCTT TCAGCAAAAA ACCCCTCAAG ACCCGTTTAG 121 AGGCCCCAAG GGGTTATGCT AGTTATTGCT CAGCGGTGGC AGCAGCCAAC TCAGCTTCCT 181 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT AGGAGTGGGC CACGGCGTCC 241 TCCACCTGCT CGGTGATGTT GCCCTCGGTC TTCCTCACCA GCCTGTGGCC GATGTAGTGG 301 TCGCCGGGCA TCTCCAGGAT CTCCTTGGCG GCCTTGTAGG TGGTCTTCAT CTGGCACTTG 361 TGGTTGCCGC CGCCCTCCAG CTTCAGGTAC ATGGTCACGT CGCCCTTCAG CACGCCGTCG 421 CTGGCGGTGA TCTTCTCGGT GGAGGGCTCC CAGTCCACGC TCTGGTTCTG CATGATGGGG 481 CCGTCGGCGG GGAAGTTCAC GCCGGTGAAC TTGGACTTGT GGTAGAAGGT GTTGCCCCTC 541 AGGCTGATGT GGGCGCTCAC GGAGGCGGAG CCGCCGTCCT CGAACTCCAG GGACCTCTCC 601 CAGGACAGGC CCTCAGGAAA GGCCTGCTTG AAATAGTCTG GGATCTCTTC TGGGTACTTG 661 GTAAAAACTC TGTGGCCGTA ACAGAACACG TGGGACACCA GGTCGAANGC GAAAGGCATT 721 GGCCCGCCCT CGGCCATTGT GACGCGCAGT GTCATCTCCT GATGTCCCTC GTAAGGTCTG 781 CCTGTGCCCT CACCCTCGAT TGTGAACTCA TGCCCATTGA CGGANCCGTC CATGTAGTAC 841 CTCATCTTCA TCTCGGGCTT GATCACGCTC NCNANGGNCG GATNNTTATC GNCNTCGTCG 901 TACAGATNCN GANCNNTTTN CTGNNCACNN GTCATGCNAN CNNTANNNTG ANGATGATGA 961 TGATGANAAN CNNNNNATAT GNATATCTNN NNNNTNAANN NNNANNNAAA NNTNNNTTNN 1021 NNNNNNNANN NNNNNNNN //

hmKO2/pRSETB

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QC test results

References