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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmKO2/pRSETB

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.

Catalog number RDB15313
Resource name hmKO2/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
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External Database
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15313 hmKO2/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The hmKO2/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15313).

            Reference section:

            Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15313_A7Htp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp ()
            Region: insert
            Sequence file: RDB15313_A7Hta.seq check
            >D04498J4_A7H9_1_T7_20bp_E08_14_ABI24.ab1
                1 GAAAATCAGT AGAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT
               61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT
              121 CTGTACGACG ATGACGATAA GGATCCGACC ATGGTGAGCG TGATCAAGCC CGAGATGAAG
              181 ATGAGGTACT ACATGGACGG CTCCGTCAAT GGGCATGAGT TCACAATCGA GGGTGAGGGC
              241 ACAGGCAGAC CTTACGAGGG ACATCAGGAG ATGACACTGC GCGTCACAAT GGCCGAGGGC
              301 GGGCCAATGC CTTTCGCCTT CGACCTGGTG TCCCACGTGT TCTGTTACGG CCACAGAGTT
              361 TTTACCAAGT ACCCAGAAGA GATCCCAGAC TATTTCAAGC AGGCCTTTCC TGAGGGCCTG
              421 TCCTGGGAGA GGTCCCTGGA GTTCGAGGAC GGCGGCTCCG CCTCCGTGAG CGCCCACATC
              481 AGCCTGAGGG GCAACACCTT CTACCACAAG TCCAAGTTCA CCGGCGTGAA CTTCCCCGCC
              541 GACGGCCCCA TCATGCAGAA CCAGAGCGTG GACTGGGAGC CCTCCACCGA GAAGATCACC
              601 GCCAGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGA GGGCGGCGGC
              661 AACCACAAGT GCCAGATGAA GACCACCTAC AAGGCCGCCA AGGAGATCCT GGAGATGCCC
              721 GGCGACCACT ACATCGGCCA CAGGCTGGTG AGGAAGACCG AGGGCAACAT CACCGAGCAG
              781 GTGGAGGACG CCGTGGCCCA CTCCTAAGAA TTCGAAGCTT GATCCGGCTG CTAACAAAGC
              841 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
              901 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATCTGG
              961 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTTGCGCA GCCCTGAAAT
             1021 GGGCGAAATG GGACGCGCCC CTGTAGCGGC GCATTAGGCC GCCGGCCGGT TGTGGTGGGT
            1081 TACGCCGCAG CGTTGAACGC TTACACTTGC CAGCCGTCCC TTTAGACGAT CTTCA
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl