Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmKO2/pRSETB (#RDB15313)

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.

Clone info. Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15313 hmKO2/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hmKO2/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15313).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15313_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261)
Region: 6xHis,T7 Tag,Xpress tag,insert 5'
Sequence file: RDB15313_A7H9b.seq check
>D04498J4_A7H9_1_T7_20bp_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTT TGTTNNNCTN NNNNAAGGAG NNATACATAT GCGGGGTTCT
   61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT
  121 CTGTACGACG ATGACGATAA GGATCCGACC ATGGTGAGCG TGATCAAGCC CGAGATGAAG
  181 ATGAGGTACT ACATGGACGG CTCCGTCAAT GGGCATGAGT TCACAATCGA GGGTGAGGGC
  241 ACAGGCAGAC CTTACGAGGG ACATCAGGAG ATGACACTGC GCGTCACAAT GGCCGAGGGC
  301 GGGCCAATGC CTTTCGCCTT CGACCTGGTG TCCCACGTGT TCTGTTACGG CCACAGAGTT
  361 TTTACCAAGT ACCCAGAAGA GATCCCAGAC TATTTCAAGC AGGCCTTTCC TGAGGGCCTG
  421 TCCTGGGAGA GGTCCCTGGA GTTCGAGGAC GGCGGCTCCG CCTCCGTGAG CGCCCACATC
  481 AGCCTGAGGG GCAACACCTT CTACCACAAG TCCAAGTTCA CCGGCGTGAA CTTCCCCGCC
  541 GACGGCCCCA TCATGCAGAA CCAGAGCGTG GACTGGGAGC CCTCCACCGA GAAGATCACC
  601 GCCAGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGA GGGCGGCGGC
  661 AACCACAAGT GCCAGATGAA GACCACCTAC AAGGCCGCCA AGGAGATCCT GGAGATGCCC
  721 GGCGANCACT ACATCGGCCA CAGGCTGGTG ANGAAGANCG AGGGCAACAT CACCGANCAN
  781 GTGGANGACG CCGTGGCCCA CTCCTAAGAN TTCGAAGCTT GNTCCGGCTG CTNACAAAGC
  841 CCGAAAGGAA GCTGANTTGG CTGCTGCCAC CGCNNANCAA TAACTAGCAT AACCCCTTGG
  901 GGCCTCTNAA CGGGNCTNGA GGGGTTTTTN GCTGAAAGGA NGNNNTATAT CCGGNNNNGG
961 NGTAANANCN NNNNNGNCNN CANNNNTNNN CNNNNCNNNN NNNNNN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,insert 3'
Sequence file: RDB15313_A7H9c.seq check
>D04498J4_A7H9_1_f1ori_R3_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNANNNTTGG GNNNGGCGNN CGGTGCGGGC CTCTTCGCTA
   61 TTACGCCNGA TCCGGATATA GTTCCTCCTT TCAGCAAAAA ACCCCTCAAG ACCCGTTTAG
  121 AGGCCCCAAG GGGTTATGCT AGTTATTGCT CAGCGGTGGC AGCAGCCAAC TCAGCTTCCT
  181 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT AGGAGTGGGC CACGGCGTCC
  241 TCCACCTGCT CGGTGATGTT GCCCTCGGTC TTCCTCACCA GCCTGTGGCC GATGTAGTGG
  301 TCGCCGGGCA TCTCCAGGAT CTCCTTGGCG GCCTTGTAGG TGGTCTTCAT CTGGCACTTG
  361 TGGTTGCCGC CGCCCTCCAG CTTCAGGTAC ATGGTCACGT CGCCCTTCAG CACGCCGTCG
  421 CTGGCGGTGA TCTTCTCGGT GGAGGGCTCC CAGTCCACGC TCTGGTTCTG CATGATGGGG
  481 CCGTCGGCGG GGAAGTTCAC GCCGGTGAAC TTGGACTTGT GGTAGAAGGT GTTGCCCCTC
  541 AGGCTGATGT GGGCGCTCAC GGAGGCGGAG CCGCCGTCCT CGAACTCCAG GGACCTCTCC
  601 CAGGACAGGC CCTCAGGAAA GGCCTGCTTG AAATAGTCTG GGATCTCTTC TGGGTACTTG
  661 GTAAAAACTC TGTGGCCGTA ACAGAACACG TGGGACACCA GGTCGAANGC GAAAGGCATT
  721 GGCCCGCCCT CGGCCATTGT GACGCGCAGT GTCATCTCCT GATGTCCCTC GTAAGGTCTG
  781 CCTGTGCCCT CACCCTCGAT TGTGAACTCA TGCCCATTGA CGGANCCGTC CATGTAGTAC
  841 CTCATCTTCA TCTCGGGCTT GATCACGCTC NCNANGGNCG GATNNTTATC GNCNTCGTCG
  901 TACAGATNCN GANCNNTTTN CTGNNCACNN GTCATGCNAN CNNTANNNTG ANGATGATGA
  961 TGATGANAAN CNNNNNATAT GNATATCTNN NNNNTNAANN NNNANNNAAA NNTNNNTTNN
1021 NNNNNNNANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]