Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO2). A fast-folding variant of mKO.
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External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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Ordering | Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Approval form [Word] ![]() Please visit Information of Request for Distribution.[link] |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
提供条件 | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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提供依頼 | 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 宮脇敦史博士による提供承諾書 [Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
備考 | 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15313 | hmKO2/pRSETB | DNA solution |
Materials & Methods section:
The hmKO2/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15313). |
Reference section:
Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Fluorescent protein resources (English text) |
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Featured content | Fluorescent protein resources (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15313_A7Htp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: T7_20bp () Region: insert Sequence file: RDB15313_A7Hta.seq ![]() |
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>D04498J4_A7H9_1_T7_20bp_E08_14_ABI24.ab1 1 GAAAATCAGT AGAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT 61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT 121 CTGTACGACG ATGACGATAA GGATCCGACC ATGGTGAGCG TGATCAAGCC CGAGATGAAG 181 ATGAGGTACT ACATGGACGG CTCCGTCAAT GGGCATGAGT TCACAATCGA GGGTGAGGGC 241 ACAGGCAGAC CTTACGAGGG ACATCAGGAG ATGACACTGC GCGTCACAAT GGCCGAGGGC 301 GGGCCAATGC CTTTCGCCTT CGACCTGGTG TCCCACGTGT TCTGTTACGG CCACAGAGTT 361 TTTACCAAGT ACCCAGAAGA GATCCCAGAC TATTTCAAGC AGGCCTTTCC TGAGGGCCTG 421 TCCTGGGAGA GGTCCCTGGA GTTCGAGGAC GGCGGCTCCG CCTCCGTGAG CGCCCACATC 481 AGCCTGAGGG GCAACACCTT CTACCACAAG TCCAAGTTCA CCGGCGTGAA CTTCCCCGCC 541 GACGGCCCCA TCATGCAGAA CCAGAGCGTG GACTGGGAGC CCTCCACCGA GAAGATCACC 601 GCCAGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGA GGGCGGCGGC 661 AACCACAAGT GCCAGATGAA GACCACCTAC AAGGCCGCCA AGGAGATCCT GGAGATGCCC 721 GGCGACCACT ACATCGGCCA CAGGCTGGTG AGGAAGACCG AGGGCAACAT CACCGAGCAG 781 GTGGAGGACG CCGTGGCCCA CTCCTAAGAA TTCGAAGCTT GATCCGGCTG CTAACAAAGC 841 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 901 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATCTGG 961 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTTGCGCA GCCCTGAAAT 1021 GGGCGAAATG GGACGCGCCC CTGTAGCGGC GCATTAGGCC GCCGGCCGGT TGTGGTGGGT 1081 TACGCCGCAG CGTTGAACGC TTACACTTGC CAGCCGTCCC TTTAGACGAT CTTCA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP] |
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2023.08.17
GNP_filter3_RDBDEP_html_230702.pl