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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

MiCy-DEVD-mKO1/pCS2 (#RDB15240)

Expression vector of FRET based caspase3 activity monitoring probe.

Clone info. Expression vector of FRET based caspase3 activity monitoring probe. Two fluorescent proteins MiCy and mKO1 were connected by a caspase3 substrate peptide DEVD (Asp-Glu-Val-Asp).
Vector backbone pCS2 (Plasmid)
Gene/insert name Acropara sp. CFP (MiCy) cDNA Fungia concinna RFP (mKO1) cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (partial) RDB15240pta01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15240 MiCy-DEVD-mKO1/pCS2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The MiCy-DEVD-mKO1/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15240).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15240_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F (Pr0506)
Region: SP6 pro, insert 5'
Sequence file: RDB15240_A7H9a.seq check
>D04498E7_2_A7H9_pcmv_IE94_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGGGNNG NCTATATAAG CAATGCTCGT TTAGGGAACC
   61 GCCATTCTGC CTGGGGACGT CGGAGCAAGC TTGATTTAGG TGACACTATA GAATACAAGC
  121 TACTTGTTCT TTTTGCAGGA TCCGACCATG GTGTCTTATT CAAAGCAAGG CATCGCACAA
  181 GAAATGCGGA CGAAATACCG TATGGAAGGC AGTGTCAATG GCCATGAATT CACGATCGAA
  241 GGTGTAGGAA CTGGAAACCC TTACGAAGGG AAACAGATGT CCGAATTAGT GATCATCAAG
  301 CCTAAGGGAA AACCCCTTCC ATTCTCCTTT GACATACTGT CAACAGCCTT TCAATATGGA
  361 AACAGATGCT TCACAAAGTA CCCTGCAGAC ATGCCTGACT ATTTCAAGCA AGCATTCCCA
  421 GATGGAATGT CATATGAAAG GTCATTTCTA TTTGAGGATG GAGGAGTTGC TACAGCCAGC
  481 TGGAGCATTC GTCTCGAAGG AAATTGCTTC ATCCACAATT CCATCTATCA TGGCGTAAAC
  541 TTTCCCGCTG ATGGACCCGT AATGAAGAAG CAGACAATTG GCTGGGATAA GTCCTTCGAA
  601 AAAATGAGTG TGGCTAAAGA GGTGCTAAGA GGTGATGTGA CTCAGTTTCT TCTGCTCGAA
  661 GGAGGTGGTT ACCAGAGATG CCGGTTTCAC TCCACTTACA AAACGGAGAA GCCAGTCGCA
  721 ATGCCCCCGA GTCATGTCGT ANAACATCAA ATTGTGAGGA NCGACCTTGG CCAAACTGCA
  781 AAAGGCTTCA NGGTCAAGCT GGAAGANCAT GCTGANGCTC ATGTTAACCC TTTGAAGGTT
  841 AAAGGTGGCA GCGGNGGCGA NGANGTGGAN GGTACCGGTG GCAGCATGGT GAGTGTGNNT
  901 AAACCAGANN NGAANANGAN GTACTACATG GNCGGNTCCG TCNATGGGCA TGAGTNCACN
  961 ATTGNANGNN GNNNNNNCAG GCAGANCTTN CNANGGANNT NNNGANNNGN NNNTANNCGT
1021 CACNNNGNNC NNNNGNNNGG NCNNNNNNNT TTNNNGNTNN NNNNNNNN
//
Primer: pAxCALNLF1 (Pr0166)
Region: T7 pro, insert 3'
Sequence file: RDB15240_A7H9b.seq check
>D04498E7-2_A7H9_pAxCALNL_F1_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGT CTGGNNCTAC GTAATACGAC TCNCTATAGT TCTAGAGGCT
   61 CGAGAGGCCT TGAATTCTTA CTCGAGGGAA TGAGCTACTG CATCTTCTAC CAGCTCAGTA
  121 ATGTTGCCTT CGGTTTTCCT GACGAGGCGA TGGCTGATGT AATGGCTTCC TGGCATTTTA
  181 AGAATCTTTT TTGCCGCCTT GTAAGTAGTC TTGAATTGGC ATTTGTGATT GCCGCCTCCT
  241 TCAAGTTTTA GGTACATCGT AACATCACCC TTCAGAACTC CGTCGCTGGC AGTAATTTTC
  301 TCGGTTGATG GCTCCCAATC AACACTTTGG TTTTGCATGA TAGGACCATC GGCAGGAAAG
  361 TTAACCCCAG TAAATTTGGA TTTGTGGTAG AAGGTGTTTC CTCTAAGGCT TATATGCGCA
  421 CTGACTGAAG CGGACCCACC ATCTTCGAAC TCCAACGACC TTTCCCATGA CAGGCCTTCA
  481 GGAAATGCTT GTTTGAAATA GTCTGGTATC TCTTCTGGAT ATTTAGTAAA AGGTCTGTGG
  541 CCGTAACAGA ACACGTGTGA CACTAAGTCA AACGCGAAAG GCATTGGCCC GCCCTTGGCC
  601 ATTGTGACGC GTAGTGTCAT CTCTTGATGT CCCTCGTAAG GTCTGCCTGT GCCTTCACCT
  661 TCAATTGTGA ACTCATGCCC ATTGACGGAG CCGTCCATGT AGTACCTCAT CTTCATCTCT
  721 GGTTTAATCA CACTCACCAT GCTGCCACCG GTACCGTCCA CCTCGTCGCC ACCGCTGCCA
  781 CCTTTAACCT TCAAAGGGTT AACATGAGCC TCAGCATGTT CTTCCAGCTT GACCTTNAAG
  841 CCTTTTGCAG TTTGNNCAAG GNCGGNCCNC ACNATTTGAT GNNCTACGAC ATGNCTCNGG
  901 GGNATTGCNA CNGNNTTCNN CNTTTNGNAA GTGGANNNNA NNNNGNNATC NNNNGNNACN
961 NNNNNCNTNN ANNANNNNAA NNNNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]