RDB15240 - MiCy-DEVD-mKO1/pCS2

Catalog number	RDB15240
Resource name	MiCy-DEVD-mKO1/pCS2
Clone info.	Expression vector of FRET based caspase3 activity monitoring probe. Two fluorescent proteins MiCy and mKO1 were connected by a caspase3 substrate peptide DEVD (Asp-Glu-Val-Asp).
Vector backbone	pCS2 (Plasmid)
Gene/insert name	Acropara sp. CFP (MiCy) cDNA Fungia concinna RFP (mKO1) cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15240	MiCy-DEVD-mKO1/pCS2	DNA solution

Electronic file	Nucleotide sequence (partial) compiled by us RDB15240pta01.seq

original	Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

Featured content	Sensor and Visualization (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: pCMV-IE94_F Region: SP6 pro, insert 5' Sequence file: RDB15240_A7H9a.seq
>D04498E7_2_A7H9_pcmv_IE94_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGGGNNG NCTATATAAG CAATGCTCGT TTAGGGAACC 61 GCCATTCTGC CTGGGGACGT CGGAGCAAGC TTGATTTAGG TGACACTATA GAATACAAGC 121 TACTTGTTCT TTTTGCAGGA TCCGACCATG GTGTCTTATT CAAAGCAAGG CATCGCACAA 181 GAAATGCGGA CGAAATACCG TATGGAAGGC AGTGTCAATG GCCATGAATT CACGATCGAA 241 GGTGTAGGAA CTGGAAACCC TTACGAAGGG AAACAGATGT CCGAATTAGT GATCATCAAG 301 CCTAAGGGAA AACCCCTTCC ATTCTCCTTT GACATACTGT CAACAGCCTT TCAATATGGA 361 AACAGATGCT TCACAAAGTA CCCTGCAGAC ATGCCTGACT ATTTCAAGCA AGCATTCCCA 421 GATGGAATGT CATATGAAAG GTCATTTCTA TTTGAGGATG GAGGAGTTGC TACAGCCAGC 481 TGGAGCATTC GTCTCGAAGG AAATTGCTTC ATCCACAATT CCATCTATCA TGGCGTAAAC 541 TTTCCCGCTG ATGGACCCGT AATGAAGAAG CAGACAATTG GCTGGGATAA GTCCTTCGAA 601 AAAATGAGTG TGGCTAAAGA GGTGCTAAGA GGTGATGTGA CTCAGTTTCT TCTGCTCGAA 661 GGAGGTGGTT ACCAGAGATG CCGGTTTCAC TCCACTTACA AAACGGAGAA GCCAGTCGCA 721 ATGCCCCCGA GTCATGTCGT ANAACATCAA ATTGTGAGGA NCGACCTTGG CCAAACTGCA 781 AAAGGCTTCA NGGTCAAGCT GGAAGANCAT GCTGANGCTC ATGTTAACCC TTTGAAGGTT 841 AAAGGTGGCA GCGGNGGCGA NGANGTGGAN GGTACCGGTG GCAGCATGGT GAGTGTGNNT 901 AAACCAGANN NGAANANGAN GTACTACATG GNCGGNTCCG TCNATGGGCA TGAGTNCACN 961 ATTGNANGNN GNNNNNNCAG GCAGANCTTN CNANGGANNT NNNGANNNGN NNNTANNCGT 1021 CACNNNGNNC NNNNGNNNGG NCNNNNNNNT TTNNNGNTNN NNNNNNNN //
Primer: pAxCALNLF1 Region: T7 pro, insert 3' Sequence file: RDB15240_A7H9b.seq
>D04498E7-2_A7H9_pAxCALNL_F1_C07_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGT CTGGNNCTAC GTAATACGAC TCNCTATAGT TCTAGAGGCT 61 CGAGAGGCCT TGAATTCTTA CTCGAGGGAA TGAGCTACTG CATCTTCTAC CAGCTCAGTA 121 ATGTTGCCTT CGGTTTTCCT GACGAGGCGA TGGCTGATGT AATGGCTTCC TGGCATTTTA 181 AGAATCTTTT TTGCCGCCTT GTAAGTAGTC TTGAATTGGC ATTTGTGATT GCCGCCTCCT 241 TCAAGTTTTA GGTACATCGT AACATCACCC TTCAGAACTC CGTCGCTGGC AGTAATTTTC 301 TCGGTTGATG GCTCCCAATC AACACTTTGG TTTTGCATGA TAGGACCATC GGCAGGAAAG 361 TTAACCCCAG TAAATTTGGA TTTGTGGTAG AAGGTGTTTC CTCTAAGGCT TATATGCGCA 421 CTGACTGAAG CGGACCCACC ATCTTCGAAC TCCAACGACC TTTCCCATGA CAGGCCTTCA 481 GGAAATGCTT GTTTGAAATA GTCTGGTATC TCTTCTGGAT ATTTAGTAAA AGGTCTGTGG 541 CCGTAACAGA ACACGTGTGA CACTAAGTCA AACGCGAAAG GCATTGGCCC GCCCTTGGCC 601 ATTGTGACGC GTAGTGTCAT CTCTTGATGT CCCTCGTAAG GTCTGCCTGT GCCTTCACCT 661 TCAATTGTGA ACTCATGCCC ATTGACGGAG CCGTCCATGT AGTACCTCAT CTTCATCTCT 721 GGTTTAATCA CACTCACCAT GCTGCCACCG GTACCGTCCA CCTCGTCGCC ACCGCTGCCA 781 CCTTTAACCT TCAAAGGGTT AACATGAGCC TCAGCATGTT CTTCCAGCTT GACCTTNAAG 841 CCTTTTGCAG TTTGNNCAAG GNCGGNCCNC ACNATTTGAT GNNCTACGAC ATGNCTCNGG 901 GGNATTGCNA CNGNNTTCNN CNTTTNGNAA GTGGANNNNA NNNNGNNATC NNNNGNNACN 961 NNNNNCNTNN ANNANNNNAA NNNNANNNNN NNNNN //