Please review the QC test results indicated by icon below as well as clone information before placing your order.

MiCy-DEVD-mKO1/pCS2

Expression vector of FRET based caspase3 activity monitoring probe.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15240_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F (Pr0506) Region: SP6 pro, insert 5' Sequence file: RDB15240_A7H9a.seq
>D04498E7_2_A7H9_pcmv_IE94_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGGGNNG NCTATATAAG CAATGCTCGT TTAGGGAACC    61 GCCATTCTGC CTGGGGACGT CGGAGCAAGC TTGATTTAGG TGACACTATA GAATACAAGC   121 TACTTGTTCT TTTTGCAGGA TCCGACCATG GTGTCTTATT CAAAGCAAGG CATCGCACAA   181 GAAATGCGGA CGAAATACCG TATGGAAGGC AGTGTCAATG GCCATGAATT CACGATCGAA   241 GGTGTAGGAA CTGGAAACCC TTACGAAGGG AAACAGATGT CCGAATTAGT GATCATCAAG   301 CCTAAGGGAA AACCCCTTCC ATTCTCCTTT GACATACTGT CAACAGCCTT TCAATATGGA   361 AACAGATGCT TCACAAAGTA CCCTGCAGAC ATGCCTGACT ATTTCAAGCA AGCATTCCCA   421 GATGGAATGT CATATGAAAG GTCATTTCTA TTTGAGGATG GAGGAGTTGC TACAGCCAGC   481 TGGAGCATTC GTCTCGAAGG AAATTGCTTC ATCCACAATT CCATCTATCA TGGCGTAAAC   541 TTTCCCGCTG ATGGACCCGT AATGAAGAAG CAGACAATTG GCTGGGATAA GTCCTTCGAA   601 AAAATGAGTG TGGCTAAAGA GGTGCTAAGA GGTGATGTGA CTCAGTTTCT TCTGCTCGAA   661 GGAGGTGGTT ACCAGAGATG CCGGTTTCAC TCCACTTACA AAACGGAGAA GCCAGTCGCA   721 ATGCCCCCGA GTCATGTCGT ANAACATCAA ATTGTGAGGA NCGACCTTGG CCAAACTGCA   781 AAAGGCTTCA NGGTCAAGCT GGAAGANCAT GCTGANGCTC ATGTTAACCC TTTGAAGGTT   841 AAAGGTGGCA GCGGNGGCGA NGANGTGGAN GGTACCGGTG GCAGCATGGT GAGTGTGNNT   901 AAACCAGANN NGAANANGAN GTACTACATG GNCGGNTCCG TCNATGGGCA TGAGTNCACN   961 ATTGNANGNN GNNNNNNCAG GCAGANCTTN CNANGGANNT NNNGANNNGN NNNTANNCGT 1021 CACNNNGNNC NNNNGNNNGG NCNNNNNNNT TTNNNGNTNN NNNNNNNN //
Primer: pAxCALNLF1 (Pr0166) Region: T7 pro, insert 3' Sequence file: RDB15240_A7H9b.seq
>D04498E7-2_A7H9_pAxCALNL_F1_C07_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGT CTGGNNCTAC GTAATACGAC TCNCTATAGT TCTAGAGGCT    61 CGAGAGGCCT TGAATTCTTA CTCGAGGGAA TGAGCTACTG CATCTTCTAC CAGCTCAGTA   121 ATGTTGCCTT CGGTTTTCCT GACGAGGCGA TGGCTGATGT AATGGCTTCC TGGCATTTTA   181 AGAATCTTTT TTGCCGCCTT GTAAGTAGTC TTGAATTGGC ATTTGTGATT GCCGCCTCCT   241 TCAAGTTTTA GGTACATCGT AACATCACCC TTCAGAACTC CGTCGCTGGC AGTAATTTTC   301 TCGGTTGATG GCTCCCAATC AACACTTTGG TTTTGCATGA TAGGACCATC GGCAGGAAAG   361 TTAACCCCAG TAAATTTGGA TTTGTGGTAG AAGGTGTTTC CTCTAAGGCT TATATGCGCA   421 CTGACTGAAG CGGACCCACC ATCTTCGAAC TCCAACGACC TTTCCCATGA CAGGCCTTCA   481 GGAAATGCTT GTTTGAAATA GTCTGGTATC TCTTCTGGAT ATTTAGTAAA AGGTCTGTGG   541 CCGTAACAGA ACACGTGTGA CACTAAGTCA AACGCGAAAG GCATTGGCCC GCCCTTGGCC   601 ATTGTGACGC GTAGTGTCAT CTCTTGATGT CCCTCGTAAG GTCTGCCTGT GCCTTCACCT   661 TCAATTGTGA ACTCATGCCC ATTGACGGAG CCGTCCATGT AGTACCTCAT CTTCATCTCT   721 GGTTTAATCA CACTCACCAT GCTGCCACCG GTACCGTCCA CCTCGTCGCC ACCGCTGCCA   781 CCTTTAACCT TCAAAGGGTT AACATGAGCC TCAGCATGTT CTTCCAGCTT GACCTTNAAG   841 CCTTTTGCAG TTTGNNCAAG GNCGGNCCNC ACNATTTGAT GNNCTACGAC ATGNCTCNGG   901 GGNATTGCNA CNGNNTTCNN CNTTTNGNAA GTGGANNNNA NNNNGNNATC NNNNGNNACN 961 NNNNNCNTNN ANNANNNNAA NNNNANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl