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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)2/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15446
Resource name tFucci(CA)2/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC212776 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15446 tFucci(CA)2/pCSII-EF DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Sequence RDB15446z.seq

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15446_A7Llp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EF1a-C_F
        Region: EF-1 alpha pro,insert 5'(mCherry 5')
        Sequence file: RDB15446_A7Llo.seq check
        >D05018C9-3_A7Ll_EF1a-C_F_A01_01_ABI24.ab1
            1 NNNNNNTNNN TTTTGANTTT GGNNCTTGGT TNNTTCTCNA GCCTCAGACA GTGGTTCAAA
           61 GTTTTTTTCT TCCNTTTCAG GTGTCGTGAA CACGCTACCG GTCTCGAGAA TTCTGCAGCG
          121 GCCGCGGATC CACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT
          181 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG
          241 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG
          301 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG
          361 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT
          421 TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT
          481 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT
          541 CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT
          601 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG
          661 GCCACTACGA CGCTGANGTC AAGANCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG
          721 GCGCCTACAA CGTCNACATC AAGTTGGANA TCACCTCCCA CAACGAGGAN TACACCATCG
          781 TGNAACAGTA CGAANGCGCC GAGGGCCGCC ACTNCACCGG CGGCNTGGAN GANCTGTACA
          841 AGGGNTATCC NTCACACNNG CGGCCGCTCN NNATGGANCA GCGCCGCGNC ACCGANTNNT
          901 TCGNGNGNCG CCGCCCCGGG CCCCCCNGNA TCGNGCCNCC CNNNCNNGNC NNGNNNNACC
        961 CCNANNCCNG NNANGNCCNN NACTNNNNNN CNNNNNNNNN N
        //
        Primer: PRE_rev
        Region: PRE,insert 3'(hGem(1/110),mVenus 3')
        Sequence file: RDB15446_A7Llp.seq check
        >D05018C9-3_A7Ll_PRE_rev_C01_07_ABI24.ab1
            1 NNNNNNNNNN NNCGNNNNNN ANCNNCNTAG TTAAGAATAC CAGTCAATCT TTCACAAATT
           61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA
          121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA
          181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG
          241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT
          301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT
          361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT
          421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCCT
          481 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
          541 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT
          601 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
          661 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT
          721 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
          781 CCAGGNTGTT GCCGTCCTCC TTGAAGTCGA TGCNNTTCAG CTCGATGCGG TTCACCAGGG
          841 TGTCGCCCTC NAACTTCACC TCGGCGCGNN TCTTGTANTT GNCGNCGNCC TTGAANAANA
          901 NGGTGCGCTC CNNGANGNAN CCNTCNGGCA TGGCGGANTN NNANAANTCN NGCTGNNNCN
          961 TGNGGNCNGG GNANCGGGCG AANCACTGNN NNNNNANCCN NNNNNNGNNN NNNNNGNNNN
        1021 NNNNNGNNNN NNNNNN
        //
        Primer: mCherry-C_F
        Region: insert mid(mCherry 3',hCdt1(1/100)Cy(-), P2A,mVenus 5')
        Sequence file: RDB15446_A7Llq.seq check
        >15446_15446_B2FgF_mCherry-M_F1_D01_04_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAGNN CATGGNNCTG GGNNGCCTCC
           61 TCCGAGCGGA NGTACCNNNN NNNNNNNNNC CTGAAGGGCG AGATCAAGCA GAGGCTGAAN
          121 CTNNNNGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC
          181 GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG
          241 GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG
          301 GACGAGCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGATGGA GCAGCGCCGC
          361 GTCACCGACT TCTTCGCGCG CCGCCGCCCC GGGCCCCCCC GCATCGCGCC GCCCAAGCTG
          421 GCCTGCCGCA CCCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC CGCTACCAGT
          481 GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC CAGGCCACCG
          541 GCCCGCGCGG CCGCGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC CCCCGAGGCC
          601 CCAGACATCC CANNCTGCCC TTCTCCGGGC CAGAAGATAA AGAAAACCGG NCGCGCCAAG
          661 CGCGGCAGCG GCGCCACCAA CTTCNNCCTG CTGAAGCAGG CCGGCGACGT GGAGGAGAAC
          721 CCCGGCCCCC GCGAATTCAT GGTGAGCNAG GGCGAGGAGC TGTTNNNCGG GGNGGTGCCC
          781 NTCNTGGTCG ANCTGGACGG NNACGTAAAC GGNNCNCAAG NTTCAGCGTG TCCGGNGANG
          841 GCGANGGCGA TGNCNACCTA CGGNAAGCTN ANCCCTGAAG CTNATCTGCN CCANCCNNNA
          901 ANNTGNCCNN NGNCNTGNNC NANCCCNNNN GACCNCCCCT GGGNTACGGC CNNNAGNGCT
        961 TNNNCCNNTN CCCCCGACCN NCNNGAAGCA GNANGANTTN NNNNNNNNN
        //
        Primer: EGFP-N_F
        Region: insert mid(mVenus 3',hGem(1/110) 5')
        Sequence file: RDB15446_A7Llr.seq check
        >15446_15446_B2FgF_EGFP-N_F_E01_05_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCTNNCG GNNNNNTGNN
           61 CCTGAAGCTG ATCTGCACCN NCNNNNNNNN TGCCCGTGCC CTGGCCCACC CTCGTGACCA
          121 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
          181 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
          241 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
          301 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
          361 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG
          421 CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC
          481 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT
          541 ACCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
          601 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC
          661 ACTGGCGGCC GCTCGAGATG AATCCCAGTA TGAAGCAGAA ACAAGAAGAA ATCAAAGAGA
          721 ATATAAAGAA TAGTTCTGTC NCANGAAGAN NTCTGAAGAT GATTCNNCCT TCTGCATCTG
          781 GATCTCTTGT TGNAAGANAA AATGANCTGT CCGCAGGCTT GTNCAAAAGG AAACATCGGA
          841 NTGANCACTT ANCATCTACN ACTTCCNNCC NTGGGGTTAT TGTCCCAGAN NCTANTGAAA
          901 ATAAAAAATC NTNGNAGGAN NCNCCNANGA NNNNTTTTGA TCNTNNGANN AANGAAAANN
          961 NNNTCCTCNC NNNATTGNNA NGNNNNNGGC ANAAAANNNG NNNAAANGNG CTNNANNNTN
        1021 NNNNTAACNN CNNNGGNNTN NNNNTNNNCN NTNANNNNNN NNNN
        //
        Primer: CMV_Forward
        Region: CMV pro,5' LTR,HIV-1 psi
        Sequence file: RDB15446_A7Lls.seq check
        >D05018C9-3_A7Ll_CMV-Forward_D01_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNTT GNNGCNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
           61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
          121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
          181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
          241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
          301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
          361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
          421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
          481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
          541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA
          601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA
          661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG
          721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGGNCCGC
          781 TGATCTTCAN ACCTGGAGGA NGAGATATGA NGGACAATTG GAGAAGTGAA TTATATAAAT
          841 ATAAAGTAGT AAAAATTGAA CCNTTANGAG TAGCACCCAC CNAGGNAAAG AGNANANTGN
          901 NGCAGANAGA AAAAAGANCA GNGGGNNTAN GANCTTTGTT CCTNGGGNTN CTTGGGNNCA
        961 GCNNNANCNN NNNGGGNNCA NCGNNNNTGN NNCNNNNNNN NNN
        //
        Primer: EF1a-N_R
        Region: EF-1 alpha pro,cPPT_CTS,RRE
        Sequence file: RDB15446_A7Llt.seq check
        >15446_15446_A7Ll_EF1a_N_R_E03_15_ABI24.ab1
            1 NNNNNNNNNN NNNNNNGCGN NGTGCGCTCT GCCNNCTGNC GGGCACCGGA NCCTCACGAA
           61 GCTTGAATTA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT
          121 TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT
          181 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC
          241 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT
          301 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG
          361 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC
          421 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG
          481 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT
          541 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT
          601 TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT
          661 TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC
          721 AAATCCCCAG GANCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA
          781 GCTGCTTGNT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCNTCN ATANCCNTCA
          841 NCAAATTGNT CTGCTGCTGC ACTATACNNG ACAATAATTG TCTNGNNTGT ACCGTCAGCG
          901 TNNTTNACNC NNCNCCNATA GTGCTNNCNG CNNCNNCNNN NNNNCCNANN GNNNAAANCT
        961 NNNNNNNNNN
        //
        Primer: mCherry-M_F1
        Region: insert mid(mCherry 3',hcdt(1/100)Cy(-) 5')
        Sequence file: RDB15446_A7Llu.seq check
        >15446_15446_B2FgF_mCherry-M_F1_D01_04_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAGNN CATGGNNCTG GGNNGCCTCC
           61 TCCGAGCGGA NGTACCNNNN NNNNNNNNNC CTGAAGGGCG AGATCAAGCA GAGGCTGAAN
          121 CTNNNNGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC
          181 GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG
          241 GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG
          301 GACGAGCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGATGGA GCAGCGCCGC
          361 GTCACCGACT TCTTCGCGCG CCGCCGCCCC GGGCCCCCCC GCATCGCGCC GCCCAAGCTG
          421 GCCTGCCGCA CCCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC CGCTACCAGT
          481 GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC CAGGCCACCG
          541 GCCCGCGCGG CCGCGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC CCCCGAGGCC
          601 CCAGACATCC CANNCTGCCC TTCTCCGGGC CAGAAGATAA AGAAAACCGG NCGCGCCAAG
          661 CGCGGCAGCG GCGCCACCAA CTTCNNCCTG CTGAAGCAGG CCGGCGACGT GGAGGAGAAC
          721 CCCGGCCCCC GCGAATTCAT GGTGAGCNAG GGCGAGGAGC TGTTNNNCGG GGNGGTGCCC
          781 NTCNTGGTCG ANCTGGACGG NNACGTAAAC GGNNCNCAAG NTTCAGCGTG TCCGGNGANG
          841 GCGANGGCGA TGNCNACCTA CGGNAAGCTN ANCCCTGAAG CTNATCTGCN CCANCCNNNA
          901 ANNTGNCCNN NGNCNTGNNC NANCCCNNNN GACCNCCCCT GGGNTACGGC CNNNAGNGCT
        961 TNNNCCNNTN CCCCCGACCN NCNNGAAGCA GNANGANTTN NNNNNNNNN
        //
        Primer: BGH_rev2
        Region: bGH pA,3' LTR
        Sequence file: RDB15446_A7Llv.seq check
        >D05018C9-3_A7Ll_BGH_rev2_E01_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GGGGGANGGN NNNNNACAGA NGGCTGGCAA CTAGAAGGCA
           61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
          121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
          181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
          241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
          301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
          361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
          421 TCTTCTACCT TCTCTTGCTC NACTGGTACT AGCTTGTAGC ACCATCCNAA GGTCAGTGGA
          481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCNNATCAN GGAAGTANNN TTGTGTGNGG
          541 NANATCCNCN NATNAANNAN ATNNNNNCNN NNNTGGNANT GNNNTNNNNN NTNCNNNCCC
        601 CCCNTTTNNN NTNNNAANNN GGNNNNNNNN NN
        //
        Primer: SV40pro_F
        Region: SV40pro_ori,EM7 pro,Zeo,SV40p A
        Sequence file: RDB15446_A7Llw.seq check
        >D05018C9-3_A7Ll_SV40pro_F_F01_16_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNANN NGTGNNGANG NTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
           61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
          121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
          181 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG
          241 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG
          301 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG
          361 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC
          421 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG
          481 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG
          541 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
          601 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
          661 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
          721 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
          781 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCNACCT CTANCTAGAG CTTGNCGTAA
          841 TCATGGTCAT ANCTGTTTCC TGNGTGAAAT TGNTATCCGC TCNCAATTCC NCACAACATA
          901 CGANCNNGAN NCATANNTGN NAAGNCNGGG GNGNNNNNTG AGTGAGCTAA CTCANNTTNA
          961 TTGCGTTGCG CTCACNGNCC NNNTTNNAGN CNGNNANNNN GNCNNGNCNN NTGCNTNNNN
        1021 NNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.07.01

        GNP_filter3_RDBDEP_html_220520.pl