Resource data sheet
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tFucci(CA)2/pCSII-EF

Lentivirus vector construct of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15446
Resource name tFucci(CA)2/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC212776 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15446_A7Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15446_A7Lla.seq
>D05018C9-3_A7Ll_EF1a-C_F_A01_01_ABI24.ab1
    1 TAAGCATGCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA
   61 GTTTTTTTCT TCCATTTCAG GTGTCGTGAA CACGCTACCG GTCTCGAGAA TTCTGCAGCG
  121 GCCGCGGATC CACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT
  181 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG
  241 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG
  301 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG
  361 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT
  421 TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT
  481 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT
  541 CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT
  601 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG
  661 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG
  721 GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG
  781 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA
  841 AGGGATATCC ATCACACTGG CGGCCGCTCG AGATGGAGCA GCGCCGCGTC ACCGACTTCT
  901 TCGCGCGCCG CCGCCCCGGG CCCCCCCGCA TCGCGCCGCC CAAGCTGGGC CTGCCGCACC
  961 CCCAGCCCCG CCAGGGCCCG CACTCCGCGC CCCGGCCTCC GCTACCAGTG GCAGCCCGCA
 1021 AGCGCGCCCG CCCGCCCGCG CCCCCGGACG CGACCAGCAG GCCACCGGCC GGCGCGGCCG
 1081 CGCGCTGTCG ATGACGAAGT TTCCAGGCTC AGTACCTCGA AGTCCCCAGA ACATCCTAGC
1141 CTGGCCCTTT CTCTCGGGGC CCAGA
//
Primer: mCherry-C_F RDB15446_A7Llb.seq
>D05018C9-3_A7Ll_mCherry-C_F_B01_04_ABI24.ab1
    1 TACAGGAGCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC
   61 CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC
  121 CGGCGGCATG GACGAGCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGATGGA
  181 GCAGCGCCGC GTCACCGACT TCTTCGCGCG CCGCCGCCCC GGGCCCCCCC GCATCGCGCC
  241 GCCCAAGCTG GCCTGCCGCA CCCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC
  301 CGCTACCAGT GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC
  361 CAGGCCACCG GCCCGCGCGG CCGCGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC
  421 CCCCGAGGCC CCAGACATCC CAGCCTGCCC TTCTCCGGGC CAGAAGATAA AGAAAACCGG
  481 TCGCGCCAAG CGCGGCAGCG GCGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT
  541 GGAGGAGAAC CCCGGCCCCC GCGAATTCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
  601 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC
  661 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA TCTGCACCAC
  721 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG GCCTGCAGTG
  781 CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
  841 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC
  901 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT
  961 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACACAG CCACACGTCT
 1021 ATATCACCGC CGACAGCAGA AGACGGCATC AAGGCCAACT TTCAGATCCG CACACATCGA
 1081 GGACGCGGCG TGCAGCTCGA CGACACTAAC AAGCCAGAAC ACCCCCATCG GCGGAACGGC
1141 CCCCGGTTGC CTGC
//
Primer: PRE_rev RDB15446_A7Llc.seq
>D05018C9-3_A7Ll_PRE_rev_C01_07_ABI24.ab1
    1 ACGCATCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA
  121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA
  181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG
  241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT
  301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT
  361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT
  421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCCT
  481 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
  541 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT
  601 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
  661 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT
  721 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
  781 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
  841 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA
  901 TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA
  961 TGTGGTCGGG GTAGCGGGCG AAGCACTGCA GCCGTAGCCC ACGGTGGGTC ACGAGGGTGG
 1021 GCCAGGGCAC GGGCAGCTTT GCCGGTGGGT GCAGATCAGC TTCAGGTCAG CTTTGCCGTA
 1081 GTTGCATCGG CCTCGACCTC GCTGACCGCT GAACTGTATC GATTACGTCG GCCGTCAGCT
1141 CGAACCAAGG AATTGGGCA
//
Primer: CMV-Forward RDB15446_A7Lld.seq
>D05018C9-3_A7Ll_CMV-Forward_D01_10_ABI24.ab1
    1 TTTACACCTT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
  121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
  181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
  241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
  301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
  361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
  421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
  481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
  541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA
  601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA
  661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG
  721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGGGCCGC
  781 TGATCTTCAG ACCTGGAGGA GGAGATATGA GGGACAATTG GAGAAGTGAA TTATATAAAT
  841 ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC CAAGGCAAAG AGAAGAGTGG
  901 TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT CCTTGGGTTT CTTGGGAGCA
  961 GCAGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT ACAGGCCAGA CAATTATTGT
 1021 CTGGTATAGT GCAGCAGCAG GACAATTTGC TGAGGCTATT TGGAGGCCGC CAACAGCATC
 1081 TGTTGCAAAC TCACAGGTCT GGGGCATCAA GCAAGCTTCA GTCAAGGAAA CTCTGACTGT
1141 GGAAAGATAC CCCATAAAAA AAG
//
Primer: BGH_rev2 RDB15446_A7Lle.seq
>D05018C9-3_A7Ll_BGH_rev2_E01_13_ABI24.ab1
    1 TTTTGGGGGG TCAGAAGCAC GGGGGAGGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
  301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
  361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
  421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
  481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
  541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTTCAGTCCC
  601 CCCTTTTCTT TTTAAAAAGT GGCTAAAATC TACAGCTGCC TTTGTAAGTC ATTGGTCTTA
  661 AGGTACCTGA AGTGGTGACT GGGAAAACCC ACCTCCTCCT CCTCTTGGGC TTCTACCAGC
  721 CCAATCACAT TGGTAGCGCT GTATGGTACT TGGAATGGCC CAGGTTTTCA GGCCCACCGG
  781 ATCCATGCGG GGAGCGGCCC AAGGGAAATC AATCTTAAAG GCAAGGCAAA AAGCGAAAAG
  841 GCCCAAACCG GACAGAGCGC GGAAAGAAGT CCCTCGATTA GAGCCAAAAG GAATATACCA
  901 AAGAATTCCG CGAAATCGGG GGACCACGGC GACACGTGAA GAGAGTGCTT TCCAACCACA
  961 GAAGTGAGGC AAACACACCT GTACAAGGGA AGAAGGGTGA TATTCCCGGC AATAGAAAGG
 1021 GAACCGACTC TCGGAGAGAT GCATCGTGTG TTGTTACCTA CCCACACGGT GGTATGTCCA
1081 C
//
Primer: SV40pro_F RDB15446_A7Llf.seq
>D05018C9-3_A7Ll_SV40pro_F_F01_16_ABI24.ab1
    1 CCTTGTCCTG GCATCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
  121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
  181 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG
  241 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG
  301 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG
  361 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC
  421 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG
  481 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG
  541 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
  601 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
  661 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
  721 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
  781 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
  841 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
  901 CGAGCCGGAA GCATAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA
  961 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
 1021 GAATCGGCCC AACGCGCGGG GAGAGGCGGT TGCGTATGGG CGCTCTTCCG CTTCCTCGCT
 1081 CACTGACCTC CGCTGCGCCT CGGTTCGTCC GCCTGCCGCC GGAGCGGTAT CAGCCTCCCA
1141 CCTCAAAGGC CGGTTAAATA TACACCGGT
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2018.03.15

GNP_filter3_RDBDEP_html_171214.pl