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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tdKeima/pRSETB

Plasmid clone of tdKeima.

Catalog number RDB15232
Resource name tdKeima/pRSETB
Clone info. Plasmid clone of tdKeima.
Refer nucleotide sequences AB209967, AB209968 and AB209969 as references.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Montipora sp. 20 Keima fluorescent protein
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15232 tdKeima/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) compiled by us RDB15232pta01.seq

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15232_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15232_A7H9a.seq check
>15232_B0L4F_2_lacIpro-B_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGNNN CGNNTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
  121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
  181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
  241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
  301 CCATGGTGAG TGTGATCGCT AAACAAATGA CCTACAAGGT TTATATGTCA GGCACGGTCA
  361 ATGGACACTA CTTTGAGGTC GAAGGCGATG GAAAAGGAAA GCCTTACGAG GGAGAGCAGA
  421 CAGTAAAGCT CACTGTCACC AAGGGTGGAC CTCTGCCATT TGCTTGGGAT ATATTATCAC
  481 CACTGTTTCA GTACGGAAGC ATACCATTCA CCAAGTACCC TGAAGACATC CCTGATTATG
  541 TAAAGCAGTC ATTCCCTGAG GGATATACAT GGGAGAGGAC CATGAACTTT GAAGATGGTG
  601 CAGTGTGTAC TGTCAGCAAT GATTCCAGCA TCCAAGGCAA CTGTTTCATC TACAATGTCA
  661 AAATCTCTGG TACGAACTTT CCTCCCAATG GACCTGTTAT GCAGAAGAAG ACACAGGGCT
  721 GGGAACCCAG CACTGAGCGT CTCTTTGCAC GAGATGGAAT GCTGATAGGA AACGATTATA
  781 TGGCTCTGAA GTTGGAANGA GGTGGTCACT ATTTGTGTGA ATTTAAATCT ACTTACAAGG
  841 CAAAGAANCC TGTGANGATG CCAGGGTATC ACTATATTGA CCGCAAACTG GATGTAACCA
  901 GTCACAACAG GGNTTACACA TCTGTTGANC AGTGTGAAAT AGCNNTTGCA CGCCACTCTT
  961 TGCTCGGNGG CGGCAGCGGC GGNGGNANCN GCNGNNNCGG CGGNANNNNN NCCGNCATNN
1021 NCNNNNCNAN NNNNNNNNN
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB15232_A7H9b.seq check
>15232_B0L4F_2_T7ter_R1_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NTNANTGCTN NGCGGTGGCA GCAGCCAACT CAGCTTCCTT
   61 TCGGGCTTTG TTAGCAGCCG GATCAAGCTT CGAATTCTTA GCCCAACAGG CTATGCCTTG
  121 CAATGGCTAT TTCACACTGC TCAACAGATG TGTAATCCCT GTTGTGACTG GTTACATCCA
  181 GTTTGCGGTC AATATAGTGA TACCCTGGCA TCCTCACAGG CTTCTTTGCC TTGTAAGTAG
  241 ATTTAAATTC ACACAAATAG TGACCACCTC CTTCCAACTT CAGAGCCATA TAATCGTTTC
  301 CTATCAGCAT TCCATCTCGT GCAAAGAGAC GCTCAGTGCT GGGTTCCCAG CCCTGTGTCT
  361 TCTTCTGCAT AACAGGTCCA TTGGGAGGAA AGTTCGTACC AGAGATTTTG ACATTGTAGA
  421 TGAAACAGTT GCCTTGGATG CTGGAATCAT TGCTGACAGT ACACACTGCA CCATCTTCAA
  481 AGTTCATGGT CCTCTCCCAT GTATATCCCT CAGGGAATGA CTGCTTTACA TAATCAGGGA
  541 TGTCTTCAGG GTACTTGGTG AATGGTATGC TTCCGTACTG AAACAGTGGT GATAATATAT
  601 CCCAAGCAAA TGGCAGAGGT CCACCCTTGG TGACAGTGAG CTTTACTGTC TGCTCTCCCT
  661 CGTAAGGCTT TCCTTTTCCA TCGCCTTCGA CCTCAAAGTA GTGTCCATTG ACCGTGCCTG
  721 ACATATAAAC CTTGTAGGTC ATTTGCTTGG CAATGACGGA GANCATGCCG CCGCTGCCGC
  781 CGGTACCGCC GCCGCTGCCG CCACCGAGCA AAGAGTGGCG TGCAATGGCT ATTTCACACT
  841 GCTCAACAGA TGTGTAATCC CTGTTGTGAN TGGTTACATC CAGTTTGCGG TCAATATAGT
  901 GATANCCTGG CATCCTCACA NGCTTCTTTG NCNTGNAAGT ANANTTAAAN TNACACAAAT
  961 AGTGANNANC NCCTTCCNNC TTCNNANCCN TATANNCNNT TNNNATNNNC ATTNCATNNC
1021 NNGNNANNAN NNNNNCNNNN NNNGGNNNNC NNNNCNNNNN NNNNN
//
Primer: dK-N_F1
Region: insert mid
Sequence file: RDB15232_A7H9c.seq check
>15232_B0L4F_2_dK-N_F1_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNATGGNNNC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA
   61 AAGCCTTACG AGGGAGAGCA GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA
  121 TTTGCTTGGG ATATATTATC ACCACTGTTT CAGTACGGAA GCATACCATT CACCAAGTAC
  181 CCTGAAGACA TCCCTGATTA TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG
  241 ACCATGAACT TTGAAGATGG TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC
  301 AACTGTTTCA TCTACAATGT CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT
  361 ATGCAGAAGA AGACACAGGG CTGGGAACCC AGCACTGAGC GTCTCTTTGC ACGAGATGGA
  421 ATGCTGATAG GAAACGATTA TATGGCTCTG AAGTTGGAAG GAGGTGGTCA CTATTTGTGT
  481 GAATTTAAAT CTACTTACAA GGCAAAGAAG CCTGTGAGGA TGCCAGGGTA TCACTATATT
  541 GACCGCAAAC TGGATGTAAC CAGTCACAAC AGGGATTACA CATCTGTTGA GCAGTGTGAA
  601 ATAGCCATTG CACGCCACTC TTTGCTCGGT GGCGGCAGCG GCGGCGGTAC CGGCGGCAGC
  661 GGCGGCATGG TCTCCGTCAT TGCNAAGCAA ATGACCTACA AGGTTTATAT GTCAGGCACG
  721 GTCAATGGAC ACTACTTTGA GNTCGAAGGN GATGGAAAAG GAAAGCCTTA CGANGGAGAG
  781 CAGACAGTAA AGCTCACTGT CACCNAGGNT GGANCTCTGC CATTTGCTTG GGATATATTA
  841 TCACCACTGT TTCAGTACGG ANNCATANCA TTCACCANGT ACCCTGAANA NATCCNTGAT
  901 TATGTAAAGC ANTCNTTCCC TGANGGATAT ACNTGGGAGA NGANCATGAA CTTTGAANAN
  961 GGNGNAGTGT GTNNTGNCAG CNATGANNNC NGCATCCNNN NNNACTGTTT CNTCNNNNAN
1021 GNCNAAANTN NCNNGNNNNA NNNTTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.02.18

GNP_filter3_RDBDEP_html_201222.pl