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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tdKeima/pRSETB

Plasmid clone of tdKeima.

Catalog number RDB15232
Resource name tdKeima/pRSETB
Clone info. Plasmid clone of tdKeima.
Comment Refer nucleotide sequences AB209967, AB209968 and AB209969 as references.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Montipora sp. 20 Keima fluorescent protein
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Montipora sp. 20 Keima fluorescent protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15232 tdKeima/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (partial) RDB15232pta01.seq compiled by us

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15232_A7H9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
            Sequence file: RDB15232_A7H9a.seq check
            >15232_B0L4F_2_lacIpro-B_F_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNCGNNN CGNNTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
               61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
              121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
              181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
              241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
              301 CCATGGTGAG TGTGATCGCT AAACAAATGA CCTACAAGGT TTATATGTCA GGCACGGTCA
              361 ATGGACACTA CTTTGAGGTC GAAGGCGATG GAAAAGGAAA GCCTTACGAG GGAGAGCAGA
              421 CAGTAAAGCT CACTGTCACC AAGGGTGGAC CTCTGCCATT TGCTTGGGAT ATATTATCAC
              481 CACTGTTTCA GTACGGAAGC ATACCATTCA CCAAGTACCC TGAAGACATC CCTGATTATG
              541 TAAAGCAGTC ATTCCCTGAG GGATATACAT GGGAGAGGAC CATGAACTTT GAAGATGGTG
              601 CAGTGTGTAC TGTCAGCAAT GATTCCAGCA TCCAAGGCAA CTGTTTCATC TACAATGTCA
              661 AAATCTCTGG TACGAACTTT CCTCCCAATG GACCTGTTAT GCAGAAGAAG ACACAGGGCT
              721 GGGAACCCAG CACTGAGCGT CTCTTTGCAC GAGATGGAAT GCTGATAGGA AACGATTATA
              781 TGGCTCTGAA GTTGGAANGA GGTGGTCACT ATTTGTGTGA ATTTAAATCT ACTTACAAGG
              841 CAAAGAANCC TGTGANGATG CCAGGGTATC ACTATATTGA CCGCAAACTG GATGTAACCA
              901 GTCACAACAG GGNTTACACA TCTGTTGANC AGTGTGAAAT AGCNNTTGCA CGCCACTCTT
              961 TGCTCGGNGG CGGCAGCGGC GGNGGNANCN GCNGNNNCGG CGGNANNNNN NCCGNCATNN
            1021 NCNNNNCNAN NNNNNNNNN
            //
            Primer: T7ter_R1
            Region: insert 3'
            Sequence file: RDB15232_A7H9b.seq check
            >15232_B0L4F_2_T7ter_R1_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN NTNANTGCTN NGCGGTGGCA GCAGCCAACT CAGCTTCCTT
               61 TCGGGCTTTG TTAGCAGCCG GATCAAGCTT CGAATTCTTA GCCCAACAGG CTATGCCTTG
              121 CAATGGCTAT TTCACACTGC TCAACAGATG TGTAATCCCT GTTGTGACTG GTTACATCCA
              181 GTTTGCGGTC AATATAGTGA TACCCTGGCA TCCTCACAGG CTTCTTTGCC TTGTAAGTAG
              241 ATTTAAATTC ACACAAATAG TGACCACCTC CTTCCAACTT CAGAGCCATA TAATCGTTTC
              301 CTATCAGCAT TCCATCTCGT GCAAAGAGAC GCTCAGTGCT GGGTTCCCAG CCCTGTGTCT
              361 TCTTCTGCAT AACAGGTCCA TTGGGAGGAA AGTTCGTACC AGAGATTTTG ACATTGTAGA
              421 TGAAACAGTT GCCTTGGATG CTGGAATCAT TGCTGACAGT ACACACTGCA CCATCTTCAA
              481 AGTTCATGGT CCTCTCCCAT GTATATCCCT CAGGGAATGA CTGCTTTACA TAATCAGGGA
              541 TGTCTTCAGG GTACTTGGTG AATGGTATGC TTCCGTACTG AAACAGTGGT GATAATATAT
              601 CCCAAGCAAA TGGCAGAGGT CCACCCTTGG TGACAGTGAG CTTTACTGTC TGCTCTCCCT
              661 CGTAAGGCTT TCCTTTTCCA TCGCCTTCGA CCTCAAAGTA GTGTCCATTG ACCGTGCCTG
              721 ACATATAAAC CTTGTAGGTC ATTTGCTTGG CAATGACGGA GANCATGCCG CCGCTGCCGC
              781 CGGTACCGCC GCCGCTGCCG CCACCGAGCA AAGAGTGGCG TGCAATGGCT ATTTCACACT
              841 GCTCAACAGA TGTGTAATCC CTGTTGTGAN TGGTTACATC CAGTTTGCGG TCAATATAGT
              901 GATANCCTGG CATCCTCACA NGCTTCTTTG NCNTGNAAGT ANANTTAAAN TNACACAAAT
              961 AGTGANNANC NCCTTCCNNC TTCNNANCCN TATANNCNNT TNNNATNNNC ATTNCATNNC
            1021 NNGNNANNAN NNNNNCNNNN NNNGGNNNNC NNNNCNNNNN NNNNN
            //
            Primer: dK-N_F1
            Region: insert mid
            Sequence file: RDB15232_A7H9c.seq check
            >15232_B0L4F_2_dK-N_F1_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNATGGNNNC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA
               61 AAGCCTTACG AGGGAGAGCA GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA
              121 TTTGCTTGGG ATATATTATC ACCACTGTTT CAGTACGGAA GCATACCATT CACCAAGTAC
              181 CCTGAAGACA TCCCTGATTA TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG
              241 ACCATGAACT TTGAAGATGG TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC
              301 AACTGTTTCA TCTACAATGT CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT
              361 ATGCAGAAGA AGACACAGGG CTGGGAACCC AGCACTGAGC GTCTCTTTGC ACGAGATGGA
              421 ATGCTGATAG GAAACGATTA TATGGCTCTG AAGTTGGAAG GAGGTGGTCA CTATTTGTGT
              481 GAATTTAAAT CTACTTACAA GGCAAAGAAG CCTGTGAGGA TGCCAGGGTA TCACTATATT
              541 GACCGCAAAC TGGATGTAAC CAGTCACAAC AGGGATTACA CATCTGTTGA GCAGTGTGAA
              601 ATAGCCATTG CACGCCACTC TTTGCTCGGT GGCGGCAGCG GCGGCGGTAC CGGCGGCAGC
              661 GGCGGCATGG TCTCCGTCAT TGCNAAGCAA ATGACCTACA AGGTTTATAT GTCAGGCACG
              721 GTCAATGGAC ACTACTTTGA GNTCGAAGGN GATGGAAAAG GAAAGCCTTA CGANGGAGAG
              781 CAGACAGTAA AGCTCACTGT CACCNAGGNT GGANCTCTGC CATTTGCTTG GGATATATTA
              841 TCACCACTGT TTCAGTACGG ANNCATANCA TTCACCANGT ACCCTGAANA NATCCNTGAT
              901 TATGTAAAGC ANTCNTTCCC TGANGGATAT ACNTGGGAGA NGANCATGAA CTTTGAANAN
              961 GGNGNAGTGT GTNNTGNCAG CNATGANNNC NGCATCCNNN NNNACTGTTT CNTCNNNNAN
            1021 GNCNAAANTN NCNNGNNNNA NNNTTNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl