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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tdKeima/pRSETB (#RDB15232)

Plasmid clone of tdKeima.

Clone info. Plasmid clone of tdKeima.
Comment Refer nucleotide sequences AB209967, AB209968 and AB209969 as references.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name Montipora sp. 20 Keima fluorescent protein
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (partial) RDB15232pta01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15232 tdKeima/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The tdKeima/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15232).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15232_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15232_A7H9a.seq check
>15232_B0L4F_2_lacIpro-B_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGNNN CGNNTCNGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GGATCTCGAT CCCGCGAAAT
  121 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
  181 TTTAAGAAGG AGATATACAT ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA
  241 GCATGACTGG TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCCGA
  301 CCATGGTGAG TGTGATCGCT AAACAAATGA CCTACAAGGT TTATATGTCA GGCACGGTCA
  361 ATGGACACTA CTTTGAGGTC GAAGGCGATG GAAAAGGAAA GCCTTACGAG GGAGAGCAGA
  421 CAGTAAAGCT CACTGTCACC AAGGGTGGAC CTCTGCCATT TGCTTGGGAT ATATTATCAC
  481 CACTGTTTCA GTACGGAAGC ATACCATTCA CCAAGTACCC TGAAGACATC CCTGATTATG
  541 TAAAGCAGTC ATTCCCTGAG GGATATACAT GGGAGAGGAC CATGAACTTT GAAGATGGTG
  601 CAGTGTGTAC TGTCAGCAAT GATTCCAGCA TCCAAGGCAA CTGTTTCATC TACAATGTCA
  661 AAATCTCTGG TACGAACTTT CCTCCCAATG GACCTGTTAT GCAGAAGAAG ACACAGGGCT
  721 GGGAACCCAG CACTGAGCGT CTCTTTGCAC GAGATGGAAT GCTGATAGGA AACGATTATA
  781 TGGCTCTGAA GTTGGAANGA GGTGGTCACT ATTTGTGTGA ATTTAAATCT ACTTACAAGG
  841 CAAAGAANCC TGTGANGATG CCAGGGTATC ACTATATTGA CCGCAAACTG GATGTAACCA
  901 GTCACAACAG GGNTTACACA TCTGTTGANC AGTGTGAAAT AGCNNTTGCA CGCCACTCTT
  961 TGCTCGGNGG CGGCAGCGGC GGNGGNANCN GCNGNNNCGG CGGNANNNNN NCCGNCATNN
1021 NCNNNNCNAN NNNNNNNNN
//
Primer: T7ter_R1 (Pr0798)
Region: insert 3'
Sequence file: RDB15232_A7H9b.seq check
>15232_B0L4F_2_T7ter_R1_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NTNANTGCTN NGCGGTGGCA GCAGCCAACT CAGCTTCCTT
   61 TCGGGCTTTG TTAGCAGCCG GATCAAGCTT CGAATTCTTA GCCCAACAGG CTATGCCTTG
  121 CAATGGCTAT TTCACACTGC TCAACAGATG TGTAATCCCT GTTGTGACTG GTTACATCCA
  181 GTTTGCGGTC AATATAGTGA TACCCTGGCA TCCTCACAGG CTTCTTTGCC TTGTAAGTAG
  241 ATTTAAATTC ACACAAATAG TGACCACCTC CTTCCAACTT CAGAGCCATA TAATCGTTTC
  301 CTATCAGCAT TCCATCTCGT GCAAAGAGAC GCTCAGTGCT GGGTTCCCAG CCCTGTGTCT
  361 TCTTCTGCAT AACAGGTCCA TTGGGAGGAA AGTTCGTACC AGAGATTTTG ACATTGTAGA
  421 TGAAACAGTT GCCTTGGATG CTGGAATCAT TGCTGACAGT ACACACTGCA CCATCTTCAA
  481 AGTTCATGGT CCTCTCCCAT GTATATCCCT CAGGGAATGA CTGCTTTACA TAATCAGGGA
  541 TGTCTTCAGG GTACTTGGTG AATGGTATGC TTCCGTACTG AAACAGTGGT GATAATATAT
  601 CCCAAGCAAA TGGCAGAGGT CCACCCTTGG TGACAGTGAG CTTTACTGTC TGCTCTCCCT
  661 CGTAAGGCTT TCCTTTTCCA TCGCCTTCGA CCTCAAAGTA GTGTCCATTG ACCGTGCCTG
  721 ACATATAAAC CTTGTAGGTC ATTTGCTTGG CAATGACGGA GANCATGCCG CCGCTGCCGC
  781 CGGTACCGCC GCCGCTGCCG CCACCGAGCA AAGAGTGGCG TGCAATGGCT ATTTCACACT
  841 GCTCAACAGA TGTGTAATCC CTGTTGTGAN TGGTTACATC CAGTTTGCGG TCAATATAGT
  901 GATANCCTGG CATCCTCACA NGCTTCTTTG NCNTGNAAGT ANANTTAAAN TNACACAAAT
  961 AGTGANNANC NCCTTCCNNC TTCNNANCCN TATANNCNNT TNNNATNNNC ATTNCATNNC
1021 NNGNNANNAN NNNNNCNNNN NNNGGNNNNC NNNNCNNNNN NNNNN
//
Primer: dK-N_F1 (Pr0926)
Region: insert mid
Sequence file: RDB15232_A7H9c.seq check
>15232_B0L4F_2_dK-N_F1_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNATGGNNNC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA
   61 AAGCCTTACG AGGGAGAGCA GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA
  121 TTTGCTTGGG ATATATTATC ACCACTGTTT CAGTACGGAA GCATACCATT CACCAAGTAC
  181 CCTGAAGACA TCCCTGATTA TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG
  241 ACCATGAACT TTGAAGATGG TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC
  301 AACTGTTTCA TCTACAATGT CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT
  361 ATGCAGAAGA AGACACAGGG CTGGGAACCC AGCACTGAGC GTCTCTTTGC ACGAGATGGA
  421 ATGCTGATAG GAAACGATTA TATGGCTCTG AAGTTGGAAG GAGGTGGTCA CTATTTGTGT
  481 GAATTTAAAT CTACTTACAA GGCAAAGAAG CCTGTGAGGA TGCCAGGGTA TCACTATATT
  541 GACCGCAAAC TGGATGTAAC CAGTCACAAC AGGGATTACA CATCTGTTGA GCAGTGTGAA
  601 ATAGCCATTG CACGCCACTC TTTGCTCGGT GGCGGCAGCG GCGGCGGTAC CGGCGGCAGC
  661 GGCGGCATGG TCTCCGTCAT TGCNAAGCAA ATGACCTACA AGGTTTATAT GTCAGGCACG
  721 GTCAATGGAC ACTACTTTGA GNTCGAAGGN GATGGAAAAG GAAAGCCTTA CGANGGAGAG
  781 CAGACAGTAA AGCTCACTGT CACCNAGGNT GGANCTCTGC CATTTGCTTG GGATATATTA
  841 TCACCACTGT TTCAGTACGG ANNCATANCA TTCACCANGT ACCCTGAANA NATCCNTGAT
  901 TATGTAAAGC ANTCNTTCCC TGANGGATAT ACNTGGGAGA NGANCATGAA CTTTGAANAN
  961 GGNGNAGTGT GTNNTGNCAG CNATGANNNC NGCATCCNNN NNNACTGTTT CNTCNNNNAN
1021 GNCNAAANTN NCNNGNNNNA NNNTTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]