Resource data sheet
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pcDNA3/mCherry-Parkin (#RDB20336)

Expression vector with mCehrry added to the N-terminus of mouse Parkin.

Clone info. Expression vector with mCehrry added to the N-terminus of mouse Parkin.
Vector backbone pcDNA3 (plasmid)
Selectable markers Ampicillin (E. coli)
Gene/insert name mouse Prkn cDNA; Discosoma sp. RFP (mCherry) cDNA.
Depositor|Developer Miyawaki, Atsushi | Katayama, Hiroyuki |
 
Sequence (full) RDB20336hts01.seq checkAssembled from experimentally sequenced data.
Publication Katayama, H., Cell 181(5): 1176-1187 (2020). [link to RRC of NBRP]
Test sheet RDB20336_B4Fkp1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H. et al., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell, 181(5):1176-1187, 2020.　 
4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H. et al., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell, 181(5):1176-1187, 2020.　 
4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20336 pcDNA3/mCherry-Parkin DNA solution

How to cite this biological resource

Materials & Methods section:

The pcDNA3/mCherry-Parkin was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20336).

Reference section:

Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]