Please review the QC test results indicated by icon below as well as clone information before placing your order.

GEPRA-G

Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15250_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F () Sequence file: RDB15250_A7H9a.seq
>D04498F8_1_A7H9_pCMV-IE94_F8_D05_11_ABI24.ab1     1 AAGGGGCGGT GGCGTGCCTA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC    61 ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC   121 TTGTTCTTTT TGCAGGATCC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG   181 CCCATCCTGG TCGAGCTGCA GGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG   241 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG   301 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGAGCT GGGGCGTGCA GTGCTTCAGC   361 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGTCATGCC CGAAGGCTAC   421 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG   481 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG   541 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA TCAGCCACAA CGTCTATATC   601 ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA CAACATCGTG   661 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC   721 GTGAACCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC   781 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCCGCAT GCATGACAGC   841 TATGAACTGA GTCCACAGTT AGAGGAACTC ATCACCAAGG TCAGCAAAGC CCACCAGGAG   901 ACTTTTCCCT CACTCTGCCA GCTGGGCAAG TACACCACGA ACTCCAGTGC AGATCACCGG   961 GTGCAGCTGG ACCTGGGGCT GTGGGACAAG TTCAGCGAGC TGGGCCACCA AATGCATCAT  1021 CAAGATTGTG GGAGTTTGCG AAGCGGGCTG CCCTGATTTT ACAGGGCTCA GCATTGCCGA  1081 CCAGGATCAC GCTTGCTTCA TGCTGCCTTG TCCTGGGACA TCCTAATGCC GTTGGCGGGA 1141 ATCCT //
Primer: pAxCALNL_F1 () Sequence file: RDB15250_A7H9b.seq
>D04498F8-1_A7H9_pAxCALNL_F1_H09_24_ABI24.ab1     1 TTACTCATTC TTATCATGTC TGGATCTACG TAATACGACG TCACTAGTAG TTCTAGAGGC    61 TCGAGAGGCC TTGAATTCTT AGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT   121 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG   181 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT   241 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT   301 GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG   361 CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC   421 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG AGCAGCTTCA GGGTCAGCTT   481 GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC   541 GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC   601 CATGCCACCG GTACCGCCCT TGTACAGCTC GTTCATGCCT TCAGTGATCC CGGCGGCGGT   661 CAGGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGA ACAGGGCGGA   721 CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT   781 CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCCATGAGC TCCTCAAACA TCTCCGGGTT   841 CTCCAGCATC TCTCGGATCA GGGGTGGCAT CGGGCCTGGA ATCTCCATCT TCAGGGTTAT   901 AGCCCTTTCT GCTCCCTTAG TGCTGATGCC CCGGAGGTCG GTGATTTTCA TCAGCATCCT   961 TGGGAAACAT GTAGGGTTTG GCTGGGTCTC CGTCGCCGGG CATAGAGCCT CATGGCTTTC  1021 AGCAGGCCTC TGCCAGCCTG TTCCACATCT CGGCTCTTCA GGTCCATCGG TCTCCACAGA 1081 TGAGCCAGAT AGCCAACTAG //
Primer: RARgamma_LBD () Sequence file: RDB15250_A7H9c.seq
>D04498F8_1_A7H9_RARgamma_LBD_E05_14_ABI24.ab1     1 CGATTGGGGG TGGCATCGGG CCTGGAATCT CCATCTTCAG GGTTATAGCC CTTTCTGCTC    61 CCTTAGTGCT GATGCCCCGG AGGTCGGTGA TTTTCATCAG CATCCTTGGG AACATGTAGG   121 GTTGGCTGGG TCTCCGTCGC CGGGCATAGA GCCTCAGGGC TTCCAGCAGG GGCTCCTGCA   181 GCTTGTCCAC CTTCTCGGGC TCTTCCAGGT CCATTCGGTC TCCACAGATG AGGCAGATAG   241 CACTAAGTAG CCCAGTCTCG GTGTCATCCA TCTCCAGGGG CAGCAGCTGC CCGGCAAAGG   301 CAAAGACGAG GTCTGTAAGG GGCCCAAAGC CAGCATTGTG CATCTGGGTT CGGTTCAGGG   361 TCAGCCCATC CGAGAATGTC ATAGTGTCCT GCTCTGGGGT ATACCTTGTA CAGATCCGCA   421 GCATTAGGAT GTCCAGACAA GCAGCCTTGA GCAGCGTGAT CTGGTCGGCA ATGCTGAGCC   481 CTGTAAAACC AGGCAGCCGC TTCGCAAACT CCACAATCTT GATGATGCAT TTGGTGGCCA   541 GCTCGCTGAA CTTGTCCCAC AGCCCCAGGT CCAGCTGCAC CCGGTGATCT GCACTGGAGT   601 TCGTGGTGTA CTTGCCCAGC TGGCAGAGTG AGGGAAAAGT CTCCTGGTGG GCTTTGCTGA   661 CCTTGGTGAT GAGTTCCTCT AACTGTGGAC TCAGTTCATA GCTGTCATGC ATGCGGGCGG   721 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG   781 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGGTTCAC GGGGCCGTCG CCGATGGGGG   841 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CACGATGTTG TGGCGGATCT   901 TGAAGTTGGC CTTGATGCCG TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGA   961 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CCTCCCTTGA AAGTCGATGC  1021 CCTTCAGCTC GATGCGGTTT CACCAAGGGT GTCGCCTCGA ACCTTTCACT CGGCGCGGGT  1081 CTTTGTAGTG GCGGTCGGTC CTTGCAGAGA TTGCATGCGC TCTGACGATA GTCTCGGGTC 1141 CATGTGAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl