Resource data sheet
Prev.

GEPRA-G

Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.

Catalog number RDB15250
Resource name GEPRA-G
Clone info. Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.
Vector backbone pCS2+ (plasmid)
Gene/insert name mouse RARgamma cDNA Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |
 
Other clones in our bank mouse Rarg (NCBI Gene 19411) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15250 GEPRA-G DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15250_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F RDB15250_A7H9a.seq
>D04498F8_1_A7H9_pCMV-IE94_F8_D05_11_ABI24.ab1
    1 AAGGGGCGGT GGCGTGCCTA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC
   61 ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC
  121 TTGTTCTTTT TGCAGGATCC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG
  181 CCCATCCTGG TCGAGCTGCA GGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG
  241 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG
  301 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGAGCT GGGGCGTGCA GTGCTTCAGC
  361 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGTCATGCC CGAAGGCTAC
  421 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG
  481 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG
  541 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA TCAGCCACAA CGTCTATATC
  601 ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA CAACATCGTG
  661 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
  721 GTGAACCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
  781 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCCGCAT GCATGACAGC
  841 TATGAACTGA GTCCACAGTT AGAGGAACTC ATCACCAAGG TCAGCAAAGC CCACCAGGAG
  901 ACTTTTCCCT CACTCTGCCA GCTGGGCAAG TACACCACGA ACTCCAGTGC AGATCACCGG
  961 GTGCAGCTGG ACCTGGGGCT GTGGGACAAG TTCAGCGAGC TGGGCCACCA AATGCATCAT
 1021 CAAGATTGTG GGAGTTTGCG AAGCGGGCTG CCCTGATTTT ACAGGGCTCA GCATTGCCGA
 1081 CCAGGATCAC GCTTGCTTCA TGCTGCCTTG TCCTGGGACA TCCTAATGCC GTTGGCGGGA
1141 ATCCT
//
Primer: pAxCALNL_F1 RDB15250_A7H9b.seq
>D04498F8-1_A7H9_pAxCALNL_F1_H09_24_ABI24.ab1
    1 TTACTCATTC TTATCATGTC TGGATCTACG TAATACGACG TCACTAGTAG TTCTAGAGGC
   61 TCGAGAGGCC TTGAATTCTT AGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT
  121 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG
  181 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT
  241 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT
  301 GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG
  361 CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC
  421 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG AGCAGCTTCA GGGTCAGCTT
  481 GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC
  541 GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC
  601 CATGCCACCG GTACCGCCCT TGTACAGCTC GTTCATGCCT TCAGTGATCC CGGCGGCGGT
  661 CAGGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGA ACAGGGCGGA
  721 CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT
  781 CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCCATGAGC TCCTCAAACA TCTCCGGGTT
  841 CTCCAGCATC TCTCGGATCA GGGGTGGCAT CGGGCCTGGA ATCTCCATCT TCAGGGTTAT
  901 AGCCCTTTCT GCTCCCTTAG TGCTGATGCC CCGGAGGTCG GTGATTTTCA TCAGCATCCT
  961 TGGGAAACAT GTAGGGTTTG GCTGGGTCTC CGTCGCCGGG CATAGAGCCT CATGGCTTTC
 1021 AGCAGGCCTC TGCCAGCCTG TTCCACATCT CGGCTCTTCA GGTCCATCGG TCTCCACAGA
1081 TGAGCCAGAT AGCCAACTAG
//
Primer: RARbeta_LBD RDB15250_A7H9c.seq
>D04498F8_1_A7H9_RARgamma_LBD_E05_14_ABI24.ab1
    1 CGATTGGGGG TGGCATCGGG CCTGGAATCT CCATCTTCAG GGTTATAGCC CTTTCTGCTC
   61 CCTTAGTGCT GATGCCCCGG AGGTCGGTGA TTTTCATCAG CATCCTTGGG AACATGTAGG
  121 GTTGGCTGGG TCTCCGTCGC CGGGCATAGA GCCTCAGGGC TTCCAGCAGG GGCTCCTGCA
  181 GCTTGTCCAC CTTCTCGGGC TCTTCCAGGT CCATTCGGTC TCCACAGATG AGGCAGATAG
  241 CACTAAGTAG CCCAGTCTCG GTGTCATCCA TCTCCAGGGG CAGCAGCTGC CCGGCAAAGG
  301 CAAAGACGAG GTCTGTAAGG GGCCCAAAGC CAGCATTGTG CATCTGGGTT CGGTTCAGGG
  361 TCAGCCCATC CGAGAATGTC ATAGTGTCCT GCTCTGGGGT ATACCTTGTA CAGATCCGCA
  421 GCATTAGGAT GTCCAGACAA GCAGCCTTGA GCAGCGTGAT CTGGTCGGCA ATGCTGAGCC
  481 CTGTAAAACC AGGCAGCCGC TTCGCAAACT CCACAATCTT GATGATGCAT TTGGTGGCCA
  541 GCTCGCTGAA CTTGTCCCAC AGCCCCAGGT CCAGCTGCAC CCGGTGATCT GCACTGGAGT
  601 TCGTGGTGTA CTTGCCCAGC TGGCAGAGTG AGGGAAAAGT CTCCTGGTGG GCTTTGCTGA
  661 CCTTGGTGAT GAGTTCCTCT AACTGTGGAC TCAGTTCATA GCTGTCATGC ATGCGGGCGG
  721 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
  781 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGGTTCAC GGGGCCGTCG CCGATGGGGG
  841 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CACGATGTTG TGGCGGATCT
  901 TGAAGTTGGC CTTGATGCCG TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGA
  961 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CCTCCCTTGA AAGTCGATGC
 1021 CCTTCAGCTC GATGCGGTTT CACCAAGGGT GTCGCCTCGA ACCTTTCACT CGGCGCGGGT
 1081 CTTTGTAGTG GCGGTCGGTC CTTGCAGAGA TTGCATGCGC TCTGACGATA GTCTCGGGTC
1141 CATGTGAAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.
reference Ivanovska, I.L., Cross-linked matrix rigidity and soluble retinoids synergize in nuclear lamina regulation of stem cell differentiation. Mol. Biol. Cell. 28 (14): 2010-2022 (2017). PMID 28566555.
reference Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.

2019.07.04

GNP_filter3_RDBDEP_html_190431.pl