Resource data sheet
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GEPRA-G (#RDB15250)

Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.

Clone info. Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.
Vector backbone pCS2+ (plasmid)
Gene/insert name mouse RARgamma cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15250hts01.seq checkAssembled from experimentally sequenced data.
Publication Shimozono, S., Nature 496 (7445): 363-366 (2013). [link to RRC of NBRP]
Test sheet RDB15250_A8Ftp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S. et al., Nature 496 (7445): 363-366 (2013).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15250 GEPRA-G DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The GEPRA-G was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15250).

Reference section:

Shimozono, S., Iimura, T., Kitaguchi, T., Higashijima, S., Miyawaki, A., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268. [link to RRC of NBRP]
reference Ivanovska, I.L., Cross-linked matrix rigidity and soluble retinoids synergize in nuclear lamina regulation of stem cell differentiation. Mol. Biol. Cell. 28 (14): 2010-2022 (2017). PMID 28566555.
review Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.