Resource data sheet
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Dronpa3/pRSETB

Catalog number RDB15258
Resource name Dronpa3/pRSETB
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A. (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15258 Dronpa3/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ando, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15258_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15258_A7H9a.seq
>D04498G7-1_A7H9_T7_20bp_E12_15_ABI24.ab1
    1 GGAAATTCGT CTGGGATATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC
   61 TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA
  121 TCTGTACGAC GATGACGATA AGGATCCAAT GAGTGTGATT AAACCAGACA TGAAGATCAA
  181 GCTGCGTATG GAAGGCGCTG TAAATGGACA CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG
  241 GAAGCCTTTC GAGGGAAAAC AGAGTATGGA CCTTAAAGTC AAAGAAGGCG GACCTCTGCC
  301 TTTCGCCTAT GACATCTTGA CAACTGTGTT CTGTTACGGC AACAGGGTAT TCGCCAAATA
  361 CCCAGAAAAT ATAGTAGACT ATTTCAAGCA GTCGTTTCCT GAGGGCTACT CTTGGGAACG
  421 AAGCATGAAT TACGAAGACG GGGGCATTTG TAACGCGACA AACGACATAA CCCTGGATGG
  481 TGACTGTTAT ATCTATGAAA TTCGATTTGA TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT
  541 TATGCAGAAG AGGACTGTGA AATGGGAGCC ATCCACTGAG AAATTGTATG TGCGTGATGG
  601 AGTGCTGAAG GGTGATATTA ACGCCGCTCT GTCGCTTGAA GGAGGTGGCC ATTACCGATG
  661 TGACTTCAAA ACTACTTATA AAGCTAAGAA GGTTGTCCAG TTGCCAGACT ATCACTTTGT
  721 GGACCACCAC ATTGAGATTA AAAGCCACGA CAAAGATTAC AGTAATGTTA ATCTGCATGA
  781 GCACGCCGAA GCGCATTCTG AGCTGCCGAG GCAGGCCAAG TAAGAATTCG AAGCTTGATC
  841 CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC
  901 TAGCATAACC CCTTGGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA
  961 ACTATATCCG GGATCTGGCG TAATAGCGAA GAGGCCCGGC ACCGATCGCC CTTTCCCAAC
 1021 AGTTTGCGCA GCCCTGAAAT GGCGAATGGG GACGCGCCCT GTAGCGGCCG CATTAAAGCG
 1081 CGGCGGGGTG TGATGTTAAC GCCGCAGCCG TTGACCCGGC TACCACCTTG GCCCAAGGCG
1141 GAAGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059.

2019.08.08

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