Yellow cameleon calcium ion sensor clone YC4.60.
Catalog number | RDB15138 |
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Resource name | YC4.60/pRSETB |
Clone info. | Calcium ion sensor clone YC4.60 Yellow cameleon having E31Q substitution in the 1st Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid. |
Vector backbone | pRSETB (plasmid) |
Size of vector backbone | 2.9 kb |
Selectable markers | Amp^r |
Gene/insert name | X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA |
Reference of nucleotide sequence | AB178713 (DDBJ accession number) |
Depositor|Developer | Miyawaki, Atsushi | |
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Other clones in our bank | X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Approval form [Word] ![]() |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15138 | YC4.60/pRSETB | DNA solution |
Electronic file
Electronic file | Nucleotide sequence (partial) provided by the depositor RDB15138pka02.seq |
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Calcium Ion Sensor Clones (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15138_A7H9p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: lacIpro-B_F Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15138_A7H9a.seq ![]() |
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>15138_15138_B0KrF_lacIpro-B_F_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCGNNTCN GTGNGCGAGG AAGCGGAAGA NCGCCCNATA 61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA 121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT 181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG 241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC 301 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG 361 ACGGCGACGT AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT 421 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA 481 CCCTCGTGAC CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA 541 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT 601 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC 661 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA NGACGGCAAC ATCCTGGGGC 721 ACAAGCTGGA GTACAACTAC ATCAGCCACN ACGTCTATAT CACCGCCGAN NAGCAGAAGA 781 ACGGCATCAA GGCCCACTTC AAGATCCGCC ACAACATCGA GGANNGCAGC GNGCAGCTCG 841 CCNANCACTA CNNGCAGAAC ACCCCCATCG GCGACNGNCC CNNGCTGCTG NCCGANAACC 901 NCTACCNGAN CANCCAGNNC GCCCNNANCA AAGANCCCNA NNANNANCNN GNTCACATGG 961 NNCNGCNNGN NNTNNNNGNN CNGNNNNCNN CNNNNNNNNN CNNNNNNNNN N // |
Primer: T7ter_R1 Region: insert 3' Sequence file: RDB15138_A7H9b.seq ![]() |
>15138_15138_B0KrF_T7ter_R1_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGTNNNT GCTNNGCGGT GGCAGCAGNN NNNTCAGCTT 61 CCTTTCGGGC TTTGTTAGCA GCCGGATCAA GCTTCGAATT CTTACTCGAT GTTGTGGCGG 121 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG 181 CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG 241 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC 301 TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG 361 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG 421 CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG 481 ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG 541 AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC 601 AGCTCCTCGC CCTTGCTCAC CATGCCACCG CTGCCACCCT TGTACAGCTC GTCCATGCCG 661 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG 721 GGGTCTTTGC TCANGGCGGA CTGGTAGCTC ANGTAGTGGT TGTCGGGCAG CAGCACGGGG 781 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCATG 841 ANCTCCAGNG CCCCGGANCT GGANATCTTC TTGNNCCGGT NGGCAGCGCT GANGGCAATG 901 AAGTTTTTCT TCCANCGCCT CTTCCCCCCC NNTTGCNGNN NTCATTTGNA CAAACTNTTC 961 ATANNTTNNN NTNGGNNCAT CACCNNTCNN NNATNNNNNN NCNNTNNNNN NTTNNNNNAN 1021 NNNNNNNNN // |
Primer: FP-N_F1 Region: insert mid Sequence file: RDB15138_A7H9c.seq ![]() |
>15138_15138_B0KrF_FP-N_F1_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGNAGCA GCNCGACTTC TTNNNNNNNC 61 GCCNNTGCCC GAAAGGCTAC GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT 121 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA 181 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA 241 TCAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA 301 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA 361 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG 421 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG 481 CCGCCCGCAT GCATGACCAA CTGACAGAAG AGCAGATTGC AGAGTTCAAA GAAGCCTTCT 541 CATTATTCGA CAAGGATGGG GACGGCACCA TCACCACAAA GCAGCTTGGC ACCGTTATGA 601 GGTCGCTTGG ACAAAACCCA ACGGAAGCAG AATTGCAGGA TATGATCAAT GAAGTCGATG 661 CTGATGGCAA TGGAACGATT TACTTTCCTG AATTTCTTAC TATGATGGCT AGAAAAATGA 721 AGGACACAGA CAGCGAAGAG GAAATCCNAG AAGCATTCCG TGTTTTTGAC AAGGATGGGA 781 ACGGCTANNN TCAGCGCTGC TGAATTACGT CACGNNNNNN NNAACCTCGG GGGANAAGTT 841 AACAGATGAA GAAGTTGATG AAATGATAAG GGAANCAGAT ATCGATGGTG ATGGCCNAGT 901 AAACTATGAN NAGTTTGTAC NAATGATGAN NGCAAANNGG GGGGGGAANN NGNGCTNGNA 961 AGAAAAANNN TTCNTTGNCN GNNCNNNCNC NNNCNNANCN NGNNTNCAAN NANNNANCNN 1021 CCNNNCNNNN NNGNNNNNNN GNANNNNNNN NNGNNNNNNN NNN // |
Primer: YC4.6-M_F Region: insert mid Sequence file: RDB15138_A7H9d.seq ![]() |
>15138_15138_B0KrF_YC4.6-M_F_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT GANGTCGCTT GGNNNAAAAC CCNACGGANN 61 NNGAATTGCA GGATATGATC AATGAAGTCG ATGCTGATGG CAATGGAACG ATTTACTTTC 121 CTGAATTTCT TACTATGATG GCTAGAAAAA TGAAGGACAC AGACAGCGAA GAGGAAATCC 181 GAGAAGCATT CCGTGTTTTT GACAAGGATG GGAACGGCTA CATCAGCGCT GCTGAATTAC 241 GTCACGTCAT GACAAACCTC GGGGAGAAGT TAACAGATGA AGAAGTTGAT GAAATGATAA 301 GGGAAGCAGA TATCGATGGT GATGGCCAAG TAAACTATGA AGAGTTTGTA CAAATGATGA 361 CAGCAAAGGG GGGGAAGAGG CGCTGGAAGA AAAACTTCAT TGCCGTCAGC GCTGCCAACC 421 GGTTCAAGAA GATCTCCAGC TCCGGGGCAC TGGAGCTCAT GGACGGCGGC GTGCAGCTCG 481 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC 541 ACTACCTGAG CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG 601 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG 661 GTGGCAGCGG TGGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC 721 TGGTCGANCT GGACGGCGAC GTAAACGGNC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG 781 GCGANGCCNC CTACNGCAAN NCTGACCCTG AAGCTGATCT GCNNCACCGG CAAGCTGCCC 841 GTGCCCTGNC CCNNCCCTCG NGACCACCCT GGGCTACNNN CNGCAGNGCT NCNCCCGCNA 901 CCCCGACCAC ATGNANNCAG CNNGANTTCT TCNNNNTCCG CCNTGNNCGA AGGNTACNNN 961 NNNNGNANCG NANNNNCTTC TTCNNNGNNN NNNNGNNANC TANNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2021.01.12
GNP_filter3_RDBDEP_html_201222.pl