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YC4.60/pRSETB

Yellow cameleon calcium ion sensor clone YC4.60.

Catalog number RDB15138
Resource name YC4.60/pRSETB
Clone info. Calcium ion sensor clone YC4.60 Yellow cameleon having E31Q substitution in the 1st Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AB178713 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15138 YC4.60/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB15138pka02.seq

Original reference

original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15138_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15138_A7H9a.seq check
>15138_15138_B0KrF_lacIpro-B_F_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCGNNTCN GTGNGCGAGG AAGCGGAAGA NCGCCCNATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA
  121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT
  181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG
  241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC
  301 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG
  361 ACGGCGACGT AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT
  421 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA
  481 CCCTCGTGAC CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA
  541 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT
  601 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
  661 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA NGACGGCAAC ATCCTGGGGC
  721 ACAAGCTGGA GTACAACTAC ATCAGCCACN ACGTCTATAT CACCGCCGAN NAGCAGAAGA
  781 ACGGCATCAA GGCCCACTTC AAGATCCGCC ACAACATCGA GGANNGCAGC GNGCAGCTCG
  841 CCNANCACTA CNNGCAGAAC ACCCCCATCG GCGACNGNCC CNNGCTGCTG NCCGANAACC
  901 NCTACCNGAN CANCCAGNNC GCCCNNANCA AAGANCCCNA NNANNANCNN GNTCACATGG
961 NNCNGCNNGN NNTNNNNGNN CNGNNNNCNN CNNNNNNNNN CNNNNNNNNN N
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB15138_A7H9b.seq check
>15138_15138_B0KrF_T7ter_R1_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGTNNNT GCTNNGCGGT GGCAGCAGNN NNNTCAGCTT
   61 CCTTTCGGGC TTTGTTAGCA GCCGGATCAA GCTTCGAATT CTTACTCGAT GTTGTGGCGG
  121 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG
  181 CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG
  241 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
  301 TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG
  361 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG
  421 CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG
  481 ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG
  541 AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC
  601 AGCTCCTCGC CCTTGCTCAC CATGCCACCG CTGCCACCCT TGTACAGCTC GTCCATGCCG
  661 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
  721 GGGTCTTTGC TCANGGCGGA CTGGTAGCTC ANGTAGTGGT TGTCGGGCAG CAGCACGGGG
  781 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCATG
  841 ANCTCCAGNG CCCCGGANCT GGANATCTTC TTGNNCCGGT NGGCAGCGCT GANGGCAATG
  901 AAGTTTTTCT TCCANCGCCT CTTCCCCCCC NNTTGCNGNN NTCATTTGNA CAAACTNTTC
  961 ATANNTTNNN NTNGGNNCAT CACCNNTCNN NNATNNNNNN NCNNTNNNNN NTTNNNNNAN
1021 NNNNNNNNN
//
Primer: FP-N_F1
Region: insert mid
Sequence file: RDB15138_A7H9c.seq check
>15138_15138_B0KrF_FP-N_F1_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGNAGCA GCNCGACTTC TTNNNNNNNC
   61 GCCNNTGCCC GAAAGGCTAC GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT
  121 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
  181 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
  241 TCAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA
  301 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
  361 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG
  421 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
  481 CCGCCCGCAT GCATGACCAA CTGACAGAAG AGCAGATTGC AGAGTTCAAA GAAGCCTTCT
  541 CATTATTCGA CAAGGATGGG GACGGCACCA TCACCACAAA GCAGCTTGGC ACCGTTATGA
  601 GGTCGCTTGG ACAAAACCCA ACGGAAGCAG AATTGCAGGA TATGATCAAT GAAGTCGATG
  661 CTGATGGCAA TGGAACGATT TACTTTCCTG AATTTCTTAC TATGATGGCT AGAAAAATGA
  721 AGGACACAGA CAGCGAAGAG GAAATCCNAG AAGCATTCCG TGTTTTTGAC AAGGATGGGA
  781 ACGGCTANNN TCAGCGCTGC TGAATTACGT CACGNNNNNN NNAACCTCGG GGGANAAGTT
  841 AACAGATGAA GAAGTTGATG AAATGATAAG GGAANCAGAT ATCGATGGTG ATGGCCNAGT
  901 AAACTATGAN NAGTTTGTAC NAATGATGAN NGCAAANNGG GGGGGGAANN NGNGCTNGNA
  961 AGAAAAANNN TTCNTTGNCN GNNCNNNCNC NNNCNNANCN NGNNTNCAAN NANNNANCNN
1021 CCNNNCNNNN NNGNNNNNNN GNANNNNNNN NNGNNNNNNN NNN
//
Primer: YC4.6-M_F
Region: insert mid
Sequence file: RDB15138_A7H9d.seq check
>15138_15138_B0KrF_YC4.6-M_F_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT GANGTCGCTT GGNNNAAAAC CCNACGGANN
   61 NNGAATTGCA GGATATGATC AATGAAGTCG ATGCTGATGG CAATGGAACG ATTTACTTTC
  121 CTGAATTTCT TACTATGATG GCTAGAAAAA TGAAGGACAC AGACAGCGAA GAGGAAATCC
  181 GAGAAGCATT CCGTGTTTTT GACAAGGATG GGAACGGCTA CATCAGCGCT GCTGAATTAC
  241 GTCACGTCAT GACAAACCTC GGGGAGAAGT TAACAGATGA AGAAGTTGAT GAAATGATAA
  301 GGGAAGCAGA TATCGATGGT GATGGCCAAG TAAACTATGA AGAGTTTGTA CAAATGATGA
  361 CAGCAAAGGG GGGGAAGAGG CGCTGGAAGA AAAACTTCAT TGCCGTCAGC GCTGCCAACC
  421 GGTTCAAGAA GATCTCCAGC TCCGGGGCAC TGGAGCTCAT GGACGGCGGC GTGCAGCTCG
  481 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC
  541 ACTACCTGAG CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG
  601 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG
  661 GTGGCAGCGG TGGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC
  721 TGGTCGANCT GGACGGCGAC GTAAACGGNC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG
  781 GCGANGCCNC CTACNGCAAN NCTGACCCTG AAGCTGATCT GCNNCACCGG CAAGCTGCCC
  841 GTGCCCTGNC CCNNCCCTCG NGACCACCCT GGGCTACNNN CNGCAGNGCT NCNCCCGCNA
  901 CCCCGACCAC ATGNANNCAG CNNGANTTCT TCNNNNTCCG CCNTGNNCGA AGGNTACNNN
961 NNNNGNANCG NANNNNCTTC TTCNNNGNNN NNNNGNNANC TANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

2021.01.12

GNP_filter3_RDBDEP_html_201222.pl