RDB15138 - YC4.60/pRSETB

Catalog number	RDB15138
Resource name	YC4.60/pRSETB
Clone info.	Calcium ion sensor clone YC4.60 Yellow cameleon having E31Q substitution in the 1st Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Vector backbone	pRSETB (plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence	AB178713 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Other clones in our bank	X. laevis (KEGG orthology K02183) \| synthetic (KEGG orthology K00907) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15138	YC4.60/pRSETB	DNA solution

Electronic file	Nucleotide sequence (partial) provided by the depositor RDB15138pka02.seq

original	Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

Featured content	Calcium Ion Sensor Clones (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: lacIpro-B_F Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15138_A7H9a.seq
>15138_15138_B0KrF_lacIpro-B_F_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCGNNTCN GTGNGCGAGG AAGCGGAAGA NCGCCCNATA 61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA 121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT 181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG 241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC 301 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG 361 ACGGCGACGT AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT 421 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA 481 CCCTCGTGAC CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA 541 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT 601 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC 661 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA NGACGGCAAC ATCCTGGGGC 721 ACAAGCTGGA GTACAACTAC ATCAGCCACN ACGTCTATAT CACCGCCGAN NAGCAGAAGA 781 ACGGCATCAA GGCCCACTTC AAGATCCGCC ACAACATCGA GGANNGCAGC GNGCAGCTCG 841 CCNANCACTA CNNGCAGAAC ACCCCCATCG GCGACNGNCC CNNGCTGCTG NCCGANAACC 901 NCTACCNGAN CANCCAGNNC GCCCNNANCA AAGANCCCNA NNANNANCNN GNTCACATGG 961 NNCNGCNNGN NNTNNNNGNN CNGNNNNCNN CNNNNNNNNN CNNNNNNNNN N //
Primer: T7ter_R1 Region: insert 3' Sequence file: RDB15138_A7H9b.seq
>15138_15138_B0KrF_T7ter_R1_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGTNNNT GCTNNGCGGT GGCAGCAGNN NNNTCAGCTT 61 CCTTTCGGGC TTTGTTAGCA GCCGGATCAA GCTTCGAATT CTTACTCGAT GTTGTGGCGG 121 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG 181 CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG 241 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC 301 TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG 361 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG 421 CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG 481 ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG 541 AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC 601 AGCTCCTCGC CCTTGCTCAC CATGCCACCG CTGCCACCCT TGTACAGCTC GTCCATGCCG 661 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG 721 GGGTCTTTGC TCANGGCGGA CTGGTAGCTC ANGTAGTGGT TGTCGGGCAG CAGCACGGGG 781 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCATG 841 ANCTCCAGNG CCCCGGANCT GGANATCTTC TTGNNCCGGT NGGCAGCGCT GANGGCAATG 901 AAGTTTTTCT TCCANCGCCT CTTCCCCCCC NNTTGCNGNN NTCATTTGNA CAAACTNTTC 961 ATANNTTNNN NTNGGNNCAT CACCNNTCNN NNATNNNNNN NCNNTNNNNN NTTNNNNNAN 1021 NNNNNNNNN //
Primer: FP-N_F1 Region: insert mid Sequence file: RDB15138_A7H9c.seq
>15138_15138_B0KrF_FP-N_F1_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGNAGCA GCNCGACTTC TTNNNNNNNC 61 GCCNNTGCCC GAAAGGCTAC GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT 121 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA 181 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA 241 TCAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA 301 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA 361 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG 421 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG 481 CCGCCCGCAT GCATGACCAA CTGACAGAAG AGCAGATTGC AGAGTTCAAA GAAGCCTTCT 541 CATTATTCGA CAAGGATGGG GACGGCACCA TCACCACAAA GCAGCTTGGC ACCGTTATGA 601 GGTCGCTTGG ACAAAACCCA ACGGAAGCAG AATTGCAGGA TATGATCAAT GAAGTCGATG 661 CTGATGGCAA TGGAACGATT TACTTTCCTG AATTTCTTAC TATGATGGCT AGAAAAATGA 721 AGGACACAGA CAGCGAAGAG GAAATCCNAG AAGCATTCCG TGTTTTTGAC AAGGATGGGA 781 ACGGCTANNN TCAGCGCTGC TGAATTACGT CACGNNNNNN NNAACCTCGG GGGANAAGTT 841 AACAGATGAA GAAGTTGATG AAATGATAAG GGAANCAGAT ATCGATGGTG ATGGCCNAGT 901 AAACTATGAN NAGTTTGTAC NAATGATGAN NGCAAANNGG GGGGGGAANN NGNGCTNGNA 961 AGAAAAANNN TTCNTTGNCN GNNCNNNCNC NNNCNNANCN NGNNTNCAAN NANNNANCNN 1021 CCNNNCNNNN NNGNNNNNNN GNANNNNNNN NNGNNNNNNN NNN //
Primer: YC4.6-M_F Region: insert mid Sequence file: RDB15138_A7H9d.seq
>15138_15138_B0KrF_YC4.6-M_F_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT GANGTCGCTT GGNNNAAAAC CCNACGGANN 61 NNGAATTGCA GGATATGATC AATGAAGTCG ATGCTGATGG CAATGGAACG ATTTACTTTC 121 CTGAATTTCT TACTATGATG GCTAGAAAAA TGAAGGACAC AGACAGCGAA GAGGAAATCC 181 GAGAAGCATT CCGTGTTTTT GACAAGGATG GGAACGGCTA CATCAGCGCT GCTGAATTAC 241 GTCACGTCAT GACAAACCTC GGGGAGAAGT TAACAGATGA AGAAGTTGAT GAAATGATAA 301 GGGAAGCAGA TATCGATGGT GATGGCCAAG TAAACTATGA AGAGTTTGTA CAAATGATGA 361 CAGCAAAGGG GGGGAAGAGG CGCTGGAAGA AAAACTTCAT TGCCGTCAGC GCTGCCAACC 421 GGTTCAAGAA GATCTCCAGC TCCGGGGCAC TGGAGCTCAT GGACGGCGGC GTGCAGCTCG 481 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC 541 ACTACCTGAG CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG 601 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG 661 GTGGCAGCGG TGGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC 721 TGGTCGANCT GGACGGCGAC GTAAACGGNC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG 781 GCGANGCCNC CTACNGCAAN NCTGACCCTG AAGCTGATCT GCNNCACCGG CAAGCTGCCC 841 GTGCCCTGNC CCNNCCCTCG NGACCACCCT GGGCTACNNN CNGCAGNGCT NCNCCCGCNA 901 CCCCGACCAC ATGNANNCAG CNNGANTTCT TCNNNNTCCG CCNTGNNCGA AGGNTACNNN 961 NNNNGNANCG NANNNNCTTC TTCNNNGNNN NNNNGNNANC TANNNNNNNN NN //