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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

YC4.60/pRSETB

Yellow cameleon calcium ion sensor clone YC4.60.

Catalog number RDB15138
Resource name YC4.60/pRSETB
Clone info. Calcium ion sensor clone YC4.60 Yellow cameleon having E31Q substitution in the 1st Ca2+ binding site of CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB178713 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          
         
        Sequence (partial) RDB15138pka02.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15138 YC4.60/pRSETB DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15138_A7H9p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: lacIpro-B_F
        Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
        Sequence file: RDB15138_A7H9a.seq check
        >15138_15138_B0KrF_lacIpro-B_F_A08_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNCGNNTCN GTGNGCGAGG AAGCGGAAGA NCGCCCNATA
           61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA
          121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT
          181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG
          241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC
          301 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG
          361 ACGGCGACGT AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT
          421 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA
          481 CCCTCGTGAC CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA
          541 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT
          601 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
          661 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA NGACGGCAAC ATCCTGGGGC
          721 ACAAGCTGGA GTACAACTAC ATCAGCCACN ACGTCTATAT CACCGCCGAN NAGCAGAAGA
          781 ACGGCATCAA GGCCCACTTC AAGATCCGCC ACAACATCGA GGANNGCAGC GNGCAGCTCG
          841 CCNANCACTA CNNGCAGAAC ACCCCCATCG GCGACNGNCC CNNGCTGCTG NCCGANAACC
          901 NCTACCNGAN CANCCAGNNC GCCCNNANCA AAGANCCCNA NNANNANCNN GNTCACATGG
        961 NNCNGCNNGN NNTNNNNGNN CNGNNNNCNN CNNNNNNNNN CNNNNNNNNN N
        //
        Primer: T7ter_R1
        Region: insert 3'
        Sequence file: RDB15138_A7H9b.seq check
        >15138_15138_B0KrF_T7ter_R1_B08_05_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNGTNNNT GCTNNGCGGT GGCAGCAGNN NNNTCAGCTT
           61 CCTTTCGGGC TTTGTTAGCA GCCGGATCAA GCTTCGAATT CTTACTCGAT GTTGTGGCGG
          121 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG
          181 CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG
          241 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
          301 TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG
          361 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG
          421 CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG
          481 ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG
          541 AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC
          601 AGCTCCTCGC CCTTGCTCAC CATGCCACCG CTGCCACCCT TGTACAGCTC GTCCATGCCG
          661 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
          721 GGGTCTTTGC TCANGGCGGA CTGGTAGCTC ANGTAGTGGT TGTCGGGCAG CAGCACGGGG
          781 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCATG
          841 ANCTCCAGNG CCCCGGANCT GGANATCTTC TTGNNCCGGT NGGCAGCGCT GANGGCAATG
          901 AAGTTTTTCT TCCANCGCCT CTTCCCCCCC NNTTGCNGNN NTCATTTGNA CAAACTNTTC
          961 ATANNTTNNN NTNGGNNCAT CACCNNTCNN NNATNNNNNN NCNNTNNNNN NTTNNNNNAN
        1021 NNNNNNNNN
        //
        Primer: FP-N_F1
        Region: insert mid
        Sequence file: RDB15138_A7H9c.seq check
        >15138_15138_B0KrF_FP-N_F1_C08_08_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGNAGCA GCNCGACTTC TTNNNNNNNC
           61 GCCNNTGCCC GAAAGGCTAC GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT
          121 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
          181 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
          241 TCAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA
          301 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
          361 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG
          421 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
          481 CCGCCCGCAT GCATGACCAA CTGACAGAAG AGCAGATTGC AGAGTTCAAA GAAGCCTTCT
          541 CATTATTCGA CAAGGATGGG GACGGCACCA TCACCACAAA GCAGCTTGGC ACCGTTATGA
          601 GGTCGCTTGG ACAAAACCCA ACGGAAGCAG AATTGCAGGA TATGATCAAT GAAGTCGATG
          661 CTGATGGCAA TGGAACGATT TACTTTCCTG AATTTCTTAC TATGATGGCT AGAAAAATGA
          721 AGGACACAGA CAGCGAAGAG GAAATCCNAG AAGCATTCCG TGTTTTTGAC AAGGATGGGA
          781 ACGGCTANNN TCAGCGCTGC TGAATTACGT CACGNNNNNN NNAACCTCGG GGGANAAGTT
          841 AACAGATGAA GAAGTTGATG AAATGATAAG GGAANCAGAT ATCGATGGTG ATGGCCNAGT
          901 AAACTATGAN NAGTTTGTAC NAATGATGAN NGCAAANNGG GGGGGGAANN NGNGCTNGNA
          961 AGAAAAANNN TTCNTTGNCN GNNCNNNCNC NNNCNNANCN NGNNTNCAAN NANNNANCNN
        1021 CCNNNCNNNN NNGNNNNNNN GNANNNNNNN NNGNNNNNNN NNN
        //
        Primer: YC4.6-M_F
        Region: insert mid
        Sequence file: RDB15138_A7H9d.seq check
        >15138_15138_B0KrF_YC4.6-M_F_D08_11_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT GANGTCGCTT GGNNNAAAAC CCNACGGANN
           61 NNGAATTGCA GGATATGATC AATGAAGTCG ATGCTGATGG CAATGGAACG ATTTACTTTC
          121 CTGAATTTCT TACTATGATG GCTAGAAAAA TGAAGGACAC AGACAGCGAA GAGGAAATCC
          181 GAGAAGCATT CCGTGTTTTT GACAAGGATG GGAACGGCTA CATCAGCGCT GCTGAATTAC
          241 GTCACGTCAT GACAAACCTC GGGGAGAAGT TAACAGATGA AGAAGTTGAT GAAATGATAA
          301 GGGAAGCAGA TATCGATGGT GATGGCCAAG TAAACTATGA AGAGTTTGTA CAAATGATGA
          361 CAGCAAAGGG GGGGAAGAGG CGCTGGAAGA AAAACTTCAT TGCCGTCAGC GCTGCCAACC
          421 GGTTCAAGAA GATCTCCAGC TCCGGGGCAC TGGAGCTCAT GGACGGCGGC GTGCAGCTCG
          481 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC
          541 ACTACCTGAG CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG
          601 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG
          661 GTGGCAGCGG TGGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC
          721 TGGTCGANCT GGACGGCGAC GTAAACGGNC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG
          781 GCGANGCCNC CTACNGCAAN NCTGACCCTG AAGCTGATCT GCNNCACCGG CAAGCTGCCC
          841 GTGCCCTGNC CCNNCCCTCG NGACCACCCT GGGCTACNNN CNGCAGNGCT NCNCCCGCNA
          901 CCCCGACCAC ATGNANNCAG CNNGANTTCT TCNNNNTCCG CCNTGNNCGA AGGNTACNNN
        961 NNNNGNANCG NANNNNCTTC TTCNNNGNNN NNNNGNNANC TANNNNNNNN NN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428.

        2022.07.27

        GNP_filter3_RDBDEP_html_220727.pl