Resource data sheet

pcDNA3 Venus-Akaluc

Expression vector of Akaluc luciferase.

Catalog number RDB15781
Resource name pcDNA3 Venus-Akaluc
Clone info. Expression vector of Akaluc luciferase.
The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo:
(1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350.
(2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703.
Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Reference of nucleotide sequence LC320664 (DDBJ accession number)
Depositor Miyawaki, Atsushi |
Other clones in our bank Photinus pyralis (KEGG orthology K05914) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
(Japanese text [open/close])
Additional terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15781 pcDNA3 Venus-Akaluc DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Featured content

Featured content Resources for Gene Analysis (English text)


original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
reference Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
reference Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.
reference Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).