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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3 Venus-Akaluc

Expression vector of Akaluc luciferase.

Catalog number RDB15781
Resource name pcDNA3 Venus-Akaluc
Clone info. Expression vector of Akaluc luciferase.
Comment The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo:
(1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350.
(2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703.
Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Reference of insert sequence LC320664 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
firefly (Photinus pyralis) luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15781 pcDNA3 Venus-Akaluc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB15781z.seq

          Original reference

          original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
          original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0081e (English text)
          Featured content Dnaconda's recommendation EXR0081j (Japanese text)
          Featured content Higher intensity luciferase (English text)
          Featured content Higher intensity luciferase (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15781_A8B8p1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,T7 pro,insert 5' (mVenus 5')
          Sequence file: RDB15781_A8Cdg.seq check
          >D04498K3_A8B8_2_CMV-Forward_A05_02_ABI24.ab1
              1 NNNNNNNNNN NNCNTTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
            181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
            241 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA
            301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT
            361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
            421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
            481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
            541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
            601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA
            661 ACATCGAGGA NGGCGGCGTG CAGCTCGCCG ANCACTACCA GCAGAACACC CCCATCGGCG
            721 ANGGCCCCGN GCTGCTGNCC GACNNCCACT ACCTGANCTA CCAGTCCGCC CTGANCAAAG
            781 NNCCNNACGA NAAGCGCGAT CACNTGGNNC TGCTNGANTN CGNGANCGCC GCNNGGNTCA
            841 NTNTCGGCNT NGNNNANCTG TANNNNNNAT NCNNNNNANN NNGNCNAAAA NNTTAANNAN
          901 GGNCCNNNNN NNNNN
          //
          Primer: BGH_rev2
          Region: bGH pA,insert 3' (Akaluc 3')
          Sequence file: RDB15781_A8Cdh.seq check
          >D04498K3_A8B8_2_BGH_rev2_B05_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGGG GGNNGGGNAN CNACAGATGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT
            121 GCATGCTCGA GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTACAC GGCGATCTTG
            181 CCGTCCTTCT TGGCCTTAGT GAGAATCTCG CGGATCTTGC GGGCGTCTAA CTTGCCGGTC
            241 GATCCTCTAG GGACTTCATC CACAAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA
            301 ACCTGGCTGG CCACATAGTC CACGATCTCT TTCTCGGTCA TGGTTTTACC GTGTTCCAAC
            361 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACTCCGGCG
            421 TCGAAGATGT AGGGGTGTTG CAGCAGGATG CCCTCCAGTT CGGCTGGGGC TACCTGGTAG
            481 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC
            541 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTCGTAGCC
            601 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCACCGCGC
            661 TGGTTCACAC NNNGTGTCTT ANCGGTGANC AAGTCTACCA CCTTAGCNTC GAANAANGGC
            721 ACCACNTTGC CTACTGANCN NGGCTTANGG TCCNNNTNNG GGGTGATNNT GNNGGNGTTN
          781 GTTGTTTNTG TCNNNNNTAN NNNNNNNNN
          //
          Primer: mVenus-M_F
          Region: insert mid (mVenus 3', Akaluc 5')
          Sequence file: RDB15781_A8Cdi.seq check
          >15781_15781_B2EiF_mVenus-M_F_F06_18_ABI24.ab1
              1 NNNNNNNNNN NNNNANGGCN TCNAGGCNNA CTTNNAGANC CGCCACAACA TCGAGGACGG
             61 CGGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT
            121 GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
            181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
            241 CGAGCTGTAC AAGGGATCCA TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCGTT
            301 CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC
            361 CCTGGTGCCC GGCGCCATCG CCTTTACCGA CGCACATATT CAGGTGGACG TTACCTACGC
            421 CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG AGGCGCTATG GGCTGAATAC
            481 AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTCGCAG TTCTTCATGC CCGTGTTGGG
            541 TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT
            601 GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC GTGAGCAAGA AAGGGCTGCG
            661 AAAGGTCCTC AACGTGCAAA AGAAGCTACC GATCATACGA AAGATCATCA TCATGGATAG
            721 CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC GTGACTTCCC ATTTGCCACC
            781 CAGCTTCAAC GAGTACNANT TCGTGCCCGA NAGCTTCNAC CGGNNNAAAA CCATCGCCCT
            841 GATCATGAAC AGTAGTGGTA GTACAGGATT ACCCAAGGGC GTANCCCTAC CGCACCGCAC
            901 CGCNNGTGTC CNANTCNGNC NTGNCCGCGA NCCCNTCTTC NNNTNNCAGA ANNTCCCCNA
            961 NNCCGNNATC CNCAGCGNGN NNNCNTTTNN CCACGGNTTC NNNNTGNNCN NCNNGCTGGG
          1021 NNANNNGNTN NGNNGNNTNN NGNNNNNNNN NN
          //
          Primer: Venus-C_F2
          Region: insert mid (Akaluc mid)
          Sequence file: RDB15781_A8Cdj.seq check
          >D04498K3_A8B8_2_Venus-C_F2_C05_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTCTCGNN NTGGNNGAGC TGTACNAGGG NNCCATGGAA
             61 GANGCCAAAA ACATTAAGAA GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC
            121 GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCGC CATCGCCTTT
            181 ACCGACGCAC ATATTCAGGT GGACGTTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG
            241 CTGGCAGAAG CTATGAGGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC
            301 GAGAATAGCT CGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG
            361 GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG
            421 CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCGAAAGG TCCTCAACGT GCAAAAGAAG
            481 CTACCGATCA TACGAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA
            541 AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCAGCT TCAACGAGTA CGACTTCGTG
            601 CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGTAGTACA
            661 GGATTACCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC
            721 CGCGACNNCA TCTTCGGCTA CCAGAACATC CCCGACACCG CTATCCTCAG CGTGGTGCCA
            781 TTTCACCACG GCTTCNGCAT GTTCACCACG CTGGGCTANT TGNTCTGCGG CTTTCGGGTC
            841 GTGCTCATGT ACNGCTTCGA NGANGANCTA TTCTTGCGCA GCTNGCAAGA NTATAAGANT
            901 CAATCTGNCN NGCTGGNGNC CACACNATTT AGCTGCCNNG CTNAGANCNN TNNNNTCGNN
          961 NNNNNCNANC NNNNNAGCNN GNNNNANATC GNCNNNNNGN NNGNNNNNNN NNN
          //
          Primer: Akaluc 576-593
          Region: insert mid (Akaluc mid)
          Sequence file: RDB15781_A8Cdk.seq check
          >D04498K3_A8B8_2_Akaluc 576-593_D05_11_ABI24.ab1
              1 NNNNNNNNNN GNGNNNCCNT NNCGCNCCGC ACCGCTTGTG TCCGATTCNG TCATGCCCGC
             61 GACCCCATCT TCGGCTACCA GAACATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT
            121 CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG
            181 CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA
            241 TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TGCCTCGCTA AGAGCACTCT CATCGACAAG
            301 TACGACCTAA GCAGCTTGCG CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA
            361 GGTGAGGCCG TGGCCAAACG CTTCCGCCTA CCAGGCATCC GCCAGGGCTA TGGCCTGACA
            421 GAAACAACCA ACGCCGTCAT GATCACCCCC GAGGGGGACC GTAAGCCTGG CTCAGTAGGC
            481 AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTAGACTTGG TCACCGGTAA GACACTGGGT
            541 GTGAACCAGC GCGGTGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC
            601 AACCCCGAGG CTACGAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC
            661 GCCTACTGGG ACGANGACGA NCACTTCTTC ATCGTGGANC GGCTGAAGAG CCTGATCAAA
            721 TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGANGGCA TCCTGCTGCA ACACCNCTAC
            781 NTCTTCGACG CCNGANNCGC CGGCCTGNCC GANGACGATG NCGGCGANCT GCCCGCCGCA
            841 NNCGNCGTGT TNGAACACGG TAAAACCATG ANCGANAAAG ANATCGNGGN NNATGNGGCC
            901 AGCCAGGNTN NNACCGNCNN NNNNNCTGNN NNNNNGNNNN NNNNTNNNNG NNGNNNNNCN
          961 NANNNGNNNC GNNNNNNNNN N
          //
          Primer: SV40pro_F
          Region: SV40pro_ori, NeoR
          Sequence file: RDB15781_A8Cdl.seq check
          >D04498K3_A8B8_2_SV40pro_F_F05_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NANTNGTGNN GNNNNTTTTT TGGANGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGANCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGN NCGCTTTTCT GGATTCATCG ACTGTGGNCG GCTGGGTGTG GCGGANCGCT
            781 ATCANGANAT ANCGTTGGCT ACCCGTGATA TTGCTGAANA NCTTGGNNGG CNAATGGGCT
            841 GANNGCTTCC TCGNGCTTTA CNGNATCGCN GCNNCCNATN CGCAGCGCAT CGCNNNCNAT
            901 NNCNNTCNNG ANNANTTNTT CTGANCNGNN NTCTNGGGNN NCNAAATGAN NNANCNNNNG
          961 ACGCCNNNNC NNNNCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
          original Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.
          reference Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
          reference Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.
          reference Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).
          user_report Amadeo, F., Firefly luciferase offers superior performance to AkaLuc for tracking the fate of administered cell therapies. Eur. J. Nuc.l Med. Mol. Imaging 49 (3): 796-808 (2022). PMID 34313817.
          user_report Shono, Y., Protection of liver sinusoids by intravenous administration of human Muse cells in a rat extra-small partial liver transplantation model. Am. J. Transplant. 21 (6): 2025-2039 (2021). PMID 33350582.
          user_report Kayamori, K., DHODH inhibition synergizes with DNA-demethylating agents in the treatment of myelodysplastic syndromes. Blood Adv. 5 (2): 438-450 (2021). PMID 33496740.
          user_report Gaspar, N., Evaluation of NanoLuc substrates for bioluminescence imaging of transferred cells in mice. J. Photochem. Photobiol. B. 216: 112128 (2021). PMID 33529963.
          user_report Zambito, G., Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging. Mol. Imaging Biol. 22 (6): 1523-1531 (2020). PMID 32926287.
          user_report Hikita, T., In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter. Sci. Rep. 10 (1): 16616 (2020). PMID 33024173.
          user_report Zhong, C., Efficacy of the novel tubulin polymerization inhibitor PTC-028 for myelodysplastic syndrome. Cancer Sci. 111 (12): 4336-4347 (2020). PMID 33037737.
          user_report Bozec, D., Akaluc bioluminescence offers superior sensitivity to track in vivo glioma expansion. Neurooncol. Adv. 2 (1): vdaa134 (2020). PMID 33241215.

          2022.06.29

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