Resource data sheet
Prev.

pcDNA3 Venus-Akaluc

Expression vector of Akaluc luciferase.

Catalog number RDB15781
Resource name pcDNA3 Venus-Akaluc
Clone info. Expression vector of Akaluc luciferase.
The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo:
(1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350.
(2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703.
Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Reference of nucleotide sequence LC320664 (DDBJ accession number)
Depositor Miyawaki, Atsushi |
 
Other clones in our bank firefly (Photinus pyralis) (KEGG orthology K05914) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
(Japanese text [open/close])
Additional terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15781 pcDNA3 Venus-Akaluc DNA solution


References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Dnaconda's recommendation EXR0081e (English text)
Featured content Dnaconda's recommendation EXR0081j (Japanese text)

References

original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15781_A8B8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15781_A8B8a.seq
>D04498K3_A8B8_2_CMV-Forward_A05_02_ABI24.ab1
    1 CGTTACTCGG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
  181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
  241 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA
  301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT
  361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
  481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
  601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA
  661 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
  721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG
  781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
  841 CTCTCGGCAT GGACGAGCTG TACAAGGGAT CCATGGAAAG ATGCCAAAAA CATTAAGAAA
  901 GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC CGGCGAGCAG CTGCACAAAG
  961 CCCATGAAGC GCTACGCCCT GGTGCCCGGG CGCCATCGCC TTTACCGACG CACATATTCA
 1021 GGTGACGTAC CTACGCCGAG TACTTTCGAG ATGAGCGGTC GGCTGGCAGA AGGCTATGAA
 1081 GCGCTAATGG GCTGAATACA ATCGATTCCG GAATTCGTGG ATGTGCAGCG AGCAATAGGC
1141 TTTCGGCA
//
Primer: BGH_rev2 RDB15781_A8B8b.seq
>D04498K3_A8B8_2_BGH_rev2_B05_05_ABI24.ab1
    1 CATTAAGGTT AGAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT
  121 GCATGCTCGA GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTACAC GGCGATCTTG
  181 CCGTCCTTCT TGGCCTTAGT GAGAATCTCG CGGATCTTGC GGGCGTCTAA CTTGCCGGTC
  241 GATCCTCTAG GGACTTCATC CACAAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA
  301 ACCTGGCTGG CCACATAGTC CACGATCTCT TTCTCGGTCA TGGTTTTACC GTGTTCCAAC
  361 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACTCCGGCG
  421 TCGAAGATGT AGGGGTGTTG CAGCAGGATG CCCTCCAGTT CGGCTGGGGC TACCTGGTAG
  481 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC
  541 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTCGTAGCC
  601 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCACCGCGC
  661 TGGTTCACAC CCAGTGTCTT ACCGGTGACC AAGTCTACCA CCTTAGCCTC GAAGAAGGGC
  721 ACCACCTTGC CTACTGAGCC AGGCTTACGG TCCCCCTCGG GGGTGATCAT GACGGCGTTG
  781 GTTGTTTCTG TCAGCCATAG CCCTGGCGGA TGCCTGGTAG GCGGAAGCGT TTGGCCACGG
  841 CCTCACCTAC CTCCTTGCTG AGCGGCGCCC CGCCGCTGGC GATCTCGCGC AAGCTGCTTA
  901 GGTCGTACTT GTCGATGAGA GTGCTCTTAG CGAGGCAGCT AAATAGTGTG GGCACCAGCA
  961 GGCAGATTGA ATCTTATAGT CTTGCAAGCT GCGCAGATAG CTCTCTCGAA GCGGTACATG
 1021 AGCACGACCC GAAGCCGCAG ATCCAGTTAG CCAAGCGTTG TGGACATCCG AGTCGTTGCT
1081 TGAATGGCAC ACGCTGAAGG ATATCGGTTC AGAATGATCC GAGAGTA
//
Primer: Venus-C_F2 RDB15781_A8B8c.seq
>D04498K3_A8B8_2_Venus-C_F2_C05_08_ABI24.ab1
    1 AAGCTGGTAC AGCGGCGGGA CACTCTCGGC ATGGACGAGC TGTACAAGGG ATCCATGGAA
   61 GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC
  121 GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCGC CATCGCCTTT
  181 ACCGACGCAC ATATTCAGGT GGACGTTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG
  241 CTGGCAGAAG CTATGAGGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC
  301 GAGAATAGCT CGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG
  361 GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG
  421 CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCGAAAGG TCCTCAACGT GCAAAAGAAG
  481 CTACCGATCA TACGAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA
  541 AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCAGCT TCAACGAGTA CGACTTCGTG
  601 CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGTAGTACA
  661 GGATTACCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC
  721 CGCGACCCCA TCTTCGGCTA CCAGAACATC CCCGACACCG CTATCCTCAG CGTGGTGCCA
  781 TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC
  841 GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA CTATAAGATT
  901 CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTGCCTCG CTAAGAGCAC TCTCATCGAC
  961 AAGTACGACC TAAGCAGCTT GCGCGAGATC GCCCAGCGGG CGGGGCGCCC GCTCAGCAAG
 1021 GGAGGTAGGT GAGGCCGTGA CCAAACGGCT TCCGCCCTAC CAGCATTCGC AGGCTATGGA
1081 CCTGACAGAA CACGACGTCG GTTCATGATC ACTCCCCCGA AGGGGGGACC GGTTAAGGC
//
Primer: Akaluc 576-593 RDB15781_A8B8d.seq
>D04498K3_A8B8_2_Akaluc 576-593_D05_11_ABI24.ab1
    1 AGAATACACG GCGTAGCCCT ACCGCACCGC ACCGCTTGTG TCCGATTCAG TCATGCCCGC
   61 GACCCCATCT TCGGCTACCA GAACATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT
  121 CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG
  181 CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA
  241 TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TGCCTCGCTA AGAGCACTCT CATCGACAAG
  301 TACGACCTAA GCAGCTTGCG CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA
  361 GGTGAGGCCG TGGCCAAACG CTTCCGCCTA CCAGGCATCC GCCAGGGCTA TGGCCTGACA
  421 GAAACAACCA ACGCCGTCAT GATCACCCCC GAGGGGGACC GTAAGCCTGG CTCAGTAGGC
  481 AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTAGACTTGG TCACCGGTAA GACACTGGGT
  541 GTGAACCAGC GCGGTGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC
  601 AACCCCGAGG CTACGAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC
  661 GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAA
  721 TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGAGGGCA TCCTGCTGCA ACACCCCTAC
  781 ATCTTCGACG CCGGAGTCGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCCGCCGCA
  841 GTCGTCGTGT TGGAACACGG TAAAACCATG ACCGAGAAAG AGATCGTGGA CTATGTGGCC
  901 AGCCAGGTTA CAACCGCCCA AGAAGCTGCG CGGTGGTGTT GTGTTTGTGG ATGAAGTCCC
  961 TAGAGGGATC GACCGGCAAG TTAGACGCCC GCAAGATCCC GCGAGATTCT CACTAAGGCC
 1021 CAAGAAGGAC GGCAGATCGC CGTGTAGATT CTGCCAGAAT ATCAATCACA CTGACGGGCC
1081 GCTCGAGCAT GCCATCCTAG AGGGACCGTA TTTCTAATAA GTGTTCAACC CA
//
Primer: Akaluc 1215-1243 RDB15781_A8B8e.seq
>D04498K3_A8B8_2_Akaluc 1215-1243_E05_14_ABI24.ab1
    1 GACGCGGGGC GAATCGGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT
   61 GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGGGCATCCT
  121 GCTGCAACAC CCCTACATCT TCGACGCCGG AGTCGCCGGC CTGCCCGACG ACGATGCCGG
  181 CGAGCTGCCC GCCGCAGTCG TCGTGTTGGA ACACGGTAAA ACCATGACCG AGAAAGAGAT
  241 CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT
  301 TGTGGATGAA GTCCCTAGAG GATCGACCGG CAAGTTAGAC GCCCGCAAGA TCCGCGAGAT
  361 TCTCACTAAG GCCAAGAAGG ACGGCAAGAT CGCCGTGTAA GAATTCTGCA GATATCCATC
  421 ACACTGGCGG CCGCTCGAGC ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG
  481 CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC
  541 CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA
  601 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG
  661 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG
  721 GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC
  781 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC
  841 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG
  901 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTTCCG ATTTAGTGCT
  961 TTACGGCACC TCGACCCCAA AAAACTTTGA TTTACGTGAT GGGTTTCACG TAGTGGCATC
 1021 GCCCTGATAG ACGGATTTTC GGCCTTTTGG ACGGTGCGTC CACGTTCCTT ATAGTTGAAC
1081 TCTGGATCCG AACTGGACAC ACTCAATCCT ATTCTCGATC TAATTCGTTT TGAATA
//
Primer: SV40pro_F RDB15781_A8B8f.seq
>D04498K3_A8B8_2_SV40pro_F_F05_17_ABI24.ab1
    1 CTTGGGTCCC GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGGCGG CGAATGGGCT
  841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGAAATGAC CGACCAAGCG
  961 ACGCCCAACC CTGCCCATCA CGAGATTTCG ATTTCCACCG CCCGCCCTTC TATGAAAGTT
 1021 GGGCTTTCGG AATCGTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG AGATCTCATG
1081 CTTGCAGTCA CGTCAACCCC AACCTTGGTT TATTGCAGCT TTAATAATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
reference Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
reference Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.
reference Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).

2018.06.26

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