RDB15781 - pcDNA3 Venus-Akaluc

Catalog number	RDB15781
Resource name	pcDNA3 Venus-Akaluc
Clone info.	Expression vector of Akaluc luciferase. The following 2 commercial compounds have been demonstrated to be well catalyzed by Akaluc in vivo: (1) TokeOni (AkaLumine-HCl), Sigma-Aldrich. Product No.: 808350. (2) AkaLumine-HCl, Wako Pure Chemical Industry. Product No.: 012-26701, 018-26703. Patent application PCT/JP2017/030491 submitted by RIKEN that covers the creation and use of Akaluc.
Vector backbone	pcDNA3 (Plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	firefly (Photinus pyralis) luciferase cDNA
Reference of nucleotide sequence	LC320664 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Other clones in our bank	firefly (Photinus pyralis) (KEGG orthology K05914) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15781	pcDNA3 Venus-Akaluc	DNA solution

Electronic file	Sequence RDB15781z.seq

original	Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
original	Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.

Featured content	Dnaconda's recommendation EXR0081e (English text)
Featured content	Dnaconda's recommendation EXR0081j (Japanese text)
Featured content	Higher intensity luciferase (English text)
Featured content	Higher intensity luciferase (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

original	Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.
original	Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1.
reference	Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468.
reference	Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211.
reference	Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013).
user report	Zambito, G., Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging. Mol. Imaging Biol. 22 (6): 1523-1531 (2020). PMID 32926287.
user report	Hikita, T., In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter. Sci. Rep. 10 (1): 16616 (2020). PMID 33024173.

Primer: CMV_Forward Region: CMV pro,insert5'(Venus,Akaluc 5') Sequence file: RDB15781_A8B8a.seq
>D04498K3_A8B8_2_CMV-Forward_A05_02_ABI24.ab1 1 CGTTACTCGG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG 181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT 241 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA 301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT 361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG 421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG 481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG 601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA 661 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG 721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG 781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA 841 CTCTCGGCAT GGACGAGCTG TACAAGGGAT CCATGGAAAG ATGCCAAAAA CATTAAGAAA 901 GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC CGGCGAGCAG CTGCACAAAG 961 CCCATGAAGC GCTACGCCCT GGTGCCCGGG CGCCATCGCC TTTACCGACG CACATATTCA 1021 GGTGACGTAC CTACGCCGAG TACTTTCGAG ATGAGCGGTC GGCTGGCAGA AGGCTATGAA 1081 GCGCTAATGG GCTGAATACA ATCGATTCCG GAATTCGTGG ATGTGCAGCG AGCAATAGGC 1141 TTTCGGCA //
Primer: BGH_rev2 Region: bGHp(A),insert3'(Akaluc3') Sequence file: RDB15781_A8B8b.seq
>D04498K3_A8B8_2_BGH_rev2_B05_05_ABI24.ab1 1 CATTAAGGTT AGAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT 121 GCATGCTCGA GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTACAC GGCGATCTTG 181 CCGTCCTTCT TGGCCTTAGT GAGAATCTCG CGGATCTTGC GGGCGTCTAA CTTGCCGGTC 241 GATCCTCTAG GGACTTCATC CACAAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA 301 ACCTGGCTGG CCACATAGTC CACGATCTCT TTCTCGGTCA TGGTTTTACC GTGTTCCAAC 361 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACTCCGGCG 421 TCGAAGATGT AGGGGTGTTG CAGCAGGATG CCCTCCAGTT CGGCTGGGGC TACCTGGTAG 481 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC 541 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTCGTAGCC 601 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCACCGCGC 661 TGGTTCACAC CCAGTGTCTT ACCGGTGACC AAGTCTACCA CCTTAGCCTC GAAGAAGGGC 721 ACCACCTTGC CTACTGAGCC AGGCTTACGG TCCCCCTCGG GGGTGATCAT GACGGCGTTG 781 GTTGTTTCTG TCAGCCATAG CCCTGGCGGA TGCCTGGTAG GCGGAAGCGT TTGGCCACGG 841 CCTCACCTAC CTCCTTGCTG AGCGGCGCCC CGCCGCTGGC GATCTCGCGC AAGCTGCTTA 901 GGTCGTACTT GTCGATGAGA GTGCTCTTAG CGAGGCAGCT AAATAGTGTG GGCACCAGCA 961 GGCAGATTGA ATCTTATAGT CTTGCAAGCT GCGCAGATAG CTCTCTCGAA GCGGTACATG 1021 AGCACGACCC GAAGCCGCAG ATCCAGTTAG CCAAGCGTTG TGGACATCCG AGTCGTTGCT 1081 TGAATGGCAC ACGCTGAAGG ATATCGGTTC AGAATGATCC GAGAGTA //
Primer: Venus-C_F2 Region: insert mid(Venus 3',Akaluc5') Sequence file: RDB15781_A8B8c.seq
>D04498K3_A8B8_2_Venus-C_F2_C05_08_ABI24.ab1 1 AAGCTGGTAC AGCGGCGGGA CACTCTCGGC ATGGACGAGC TGTACAAGGG ATCCATGGAA 61 GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC 121 GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCGC CATCGCCTTT 181 ACCGACGCAC ATATTCAGGT GGACGTTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG 241 CTGGCAGAAG CTATGAGGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC 301 GAGAATAGCT CGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG 361 GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG 421 CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCGAAAGG TCCTCAACGT GCAAAAGAAG 481 CTACCGATCA TACGAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA 541 AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCAGCT TCAACGAGTA CGACTTCGTG 601 CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGTAGTACA 661 GGATTACCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC 721 CGCGACCCCA TCTTCGGCTA CCAGAACATC CCCGACACCG CTATCCTCAG CGTGGTGCCA 781 TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC 841 GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA CTATAAGATT 901 CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTGCCTCG CTAAGAGCAC TCTCATCGAC 961 AAGTACGACC TAAGCAGCTT GCGCGAGATC GCCCAGCGGG CGGGGCGCCC GCTCAGCAAG 1021 GGAGGTAGGT GAGGCCGTGA CCAAACGGCT TCCGCCCTAC CAGCATTCGC AGGCTATGGA 1081 CCTGACAGAA CACGACGTCG GTTCATGATC ACTCCCCCGA AGGGGGGACC GGTTAAGGC //
Primer: Akaluc 576-593 Region: insert mid(Akaluc ) Sequence file: RDB15781_A8B8d.seq
>D04498K3_A8B8_2_Akaluc 576-593_D05_11_ABI24.ab1 1 AGAATACACG GCGTAGCCCT ACCGCACCGC ACCGCTTGTG TCCGATTCAG TCATGCCCGC 61 GACCCCATCT TCGGCTACCA GAACATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT 121 CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG 181 CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA 241 TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TGCCTCGCTA AGAGCACTCT CATCGACAAG 301 TACGACCTAA GCAGCTTGCG CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA 361 GGTGAGGCCG TGGCCAAACG CTTCCGCCTA CCAGGCATCC GCCAGGGCTA TGGCCTGACA 421 GAAACAACCA ACGCCGTCAT GATCACCCCC GAGGGGGACC GTAAGCCTGG CTCAGTAGGC 481 AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTAGACTTGG TCACCGGTAA GACACTGGGT 541 GTGAACCAGC GCGGTGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC 601 AACCCCGAGG CTACGAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC 661 GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAA 721 TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGAGGGCA TCCTGCTGCA ACACCCCTAC 781 ATCTTCGACG CCGGAGTCGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCCGCCGCA 841 GTCGTCGTGT TGGAACACGG TAAAACCATG ACCGAGAAAG AGATCGTGGA CTATGTGGCC 901 AGCCAGGTTA CAACCGCCCA AGAAGCTGCG CGGTGGTGTT GTGTTTGTGG ATGAAGTCCC 961 TAGAGGGATC GACCGGCAAG TTAGACGCCC GCAAGATCCC GCGAGATTCT CACTAAGGCC 1021 CAAGAAGGAC GGCAGATCGC CGTGTAGATT CTGCCAGAAT ATCAATCACA CTGACGGGCC 1081 GCTCGAGCAT GCCATCCTAG AGGGACCGTA TTTCTAATAA GTGTTCAACC CA //
Primer: Akaluc 1215-1243 Region: insert 3' (Akaluc 3'), bGHp(A),f1 ori Sequence file: RDB15781_A8B8e.seq
>D04498K3_A8B8_2_Akaluc 1215-1243_E05_14_ABI24.ab1 1 GACGCGGGGC GAATCGGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT 61 GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGGGCATCCT 121 GCTGCAACAC CCCTACATCT TCGACGCCGG AGTCGCCGGC CTGCCCGACG ACGATGCCGG 181 CGAGCTGCCC GCCGCAGTCG TCGTGTTGGA ACACGGTAAA ACCATGACCG AGAAAGAGAT 241 CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT 301 TGTGGATGAA GTCCCTAGAG GATCGACCGG CAAGTTAGAC GCCCGCAAGA TCCGCGAGAT 361 TCTCACTAAG GCCAAGAAGG ACGGCAAGAT CGCCGTGTAA GAATTCTGCA GATATCCATC 421 ACACTGGCGG CCGCTCGAGC ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG 481 CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC 541 CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA 601 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG 661 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG 721 GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC 781 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 841 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 901 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTTCCG ATTTAGTGCT 961 TTACGGCACC TCGACCCCAA AAAACTTTGA TTTACGTGAT GGGTTTCACG TAGTGGCATC 1021 GCCCTGATAG ACGGATTTTC GGCCTTTTGG ACGGTGCGTC CACGTTCCTT ATAGTTGAAC 1081 TCTGGATCCG AACTGGACAC ACTCAATCCT ATTCTCGATC TAATTCGTTT TGAATA //
Primer: SV40pro_F Region: SV40 pro_ori,NeoR Sequence file: RDB15781_A8B8f.seq
>D04498K3_A8B8_2_SV40pro_F_F05_17_ABI24.ab1 1 CTTGGGTCCC GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGGCGG CGAATGGGCT 841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGAAATGAC CGACCAAGCG 961 ACGCCCAACC CTGCCCATCA CGAGATTTCG ATTTCCACCG CCCGCCCTTC TATGAAAGTT 1021 GGGCTTTCGG AATCGTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG AGATCTCATG 1081 CTTGCAGTCA CGTCAACCCC AACCTTGGTT TATTGCAGCT TTAATAATG //