Expression vector of Akaluc luciferase.
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Other clones in our bank
External Database firefly (Photinus pyralis) luciferase Reference sequence |
Sequence | RDB15781z.seq |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. |
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Ordering | Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Approval form [Word] ![]() Please visit Information of Request for Distribution.[link] |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
提供条件 | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。 |
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提供依頼 | 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 宮脇敦史博士による提供承諾書 [Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
備考 | 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15781 | pcDNA3 Venus-Akaluc | DNA solution |
Materials & Methods section:
The pcDNA3 Venus-Akaluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15781). |
Reference section:
Iwano, S., Sugiyama, M., Hama, H., Watakabe, A., Hasegawa, N., Kuchimaru, T., Tanaka, K.Z., Takahashi, M., Ishida, Y., Hata, J., Shimozono, S., Namiki, K., Fukano, T., Kiyama, M., Okano, H., Kizaka-Kondoh, S., McHugh, T.J., Yamamori, T., Hioki, H., Maki, S., Miyawaki, A., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [link to RRC of NBRP] |
Miyawaki, A., Iwano, S., Modified luciferase. Patent publication WO 2018/038248 A1. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Dnaconda's recommendation EXR0081e (English text) |
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Featured content | Dnaconda's recommendation EXR0081j (Japanese text) |
Featured content | Higher intensity luciferase (English text) |
Featured content | 高発光ルシフェラーゼ (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15781_A8B8p1-4.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert 5' (mVenus 5') Sequence file: RDB15781_A8Cdg.seq ![]() |
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>D04498K3_A8B8_2_CMV-Forward_A05_02_ABI24.ab1 1 NNNNNNNNNN NNCNTTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG 181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT 241 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA 301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT 361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG 421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG 481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG 601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA 661 ACATCGAGGA NGGCGGCGTG CAGCTCGCCG ANCACTACCA GCAGAACACC CCCATCGGCG 721 ANGGCCCCGN GCTGCTGNCC GACNNCCACT ACCTGANCTA CCAGTCCGCC CTGANCAAAG 781 NNCCNNACGA NAAGCGCGAT CACNTGGNNC TGCTNGANTN CGNGANCGCC GCNNGGNTCA 841 NTNTCGGCNT NGNNNANCTG TANNNNNNAT NCNNNNNANN NNGNCNAAAA NNTTAANNAN 901 GGNCCNNNNN NNNNN // |
Primer: BGH_rev2 (Pr0606) Region: bGH pA,insert 3' (Akaluc 3') Sequence file: RDB15781_A8Cdh.seq ![]() |
>D04498K3_A8B8_2_BGH_rev2_B05_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGGG GGNNGGGNAN CNACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT 121 GCATGCTCGA GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTACAC GGCGATCTTG 181 CCGTCCTTCT TGGCCTTAGT GAGAATCTCG CGGATCTTGC GGGCGTCTAA CTTGCCGGTC 241 GATCCTCTAG GGACTTCATC CACAAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA 301 ACCTGGCTGG CCACATAGTC CACGATCTCT TTCTCGGTCA TGGTTTTACC GTGTTCCAAC 361 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACTCCGGCG 421 TCGAAGATGT AGGGGTGTTG CAGCAGGATG CCCTCCAGTT CGGCTGGGGC TACCTGGTAG 481 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC 541 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTCGTAGCC 601 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCACCGCGC 661 TGGTTCACAC NNNGTGTCTT ANCGGTGANC AAGTCTACCA CCTTAGCNTC GAANAANGGC 721 ACCACNTTGC CTACTGANCN NGGCTTANGG TCCNNNTNNG GGGTGATNNT GNNGGNGTTN 781 GTTGTTTNTG TCNNNNNTAN NNNNNNNNN // |
Primer: mVenus-M_F (Pr0574) Region: insert mid (mVenus 3', Akaluc 5') Sequence file: RDB15781_A8Cdi.seq ![]() |
>15781_15781_B2EiF_mVenus-M_F_F06_18_ABI24.ab1 1 NNNNNNNNNN NNNNANGGCN TCNAGGCNNA CTTNNAGANC CGCCACAACA TCGAGGACGG 61 CGGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT 121 GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA 181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA 241 CGAGCTGTAC AAGGGATCCA TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCGTT 301 CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC 361 CCTGGTGCCC GGCGCCATCG CCTTTACCGA CGCACATATT CAGGTGGACG TTACCTACGC 421 CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG AGGCGCTATG GGCTGAATAC 481 AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTCGCAG TTCTTCATGC CCGTGTTGGG 541 TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT 601 GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC GTGAGCAAGA AAGGGCTGCG 661 AAAGGTCCTC AACGTGCAAA AGAAGCTACC GATCATACGA AAGATCATCA TCATGGATAG 721 CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC GTGACTTCCC ATTTGCCACC 781 CAGCTTCAAC GAGTACNANT TCGTGCCCGA NAGCTTCNAC CGGNNNAAAA CCATCGCCCT 841 GATCATGAAC AGTAGTGGTA GTACAGGATT ACCCAAGGGC GTANCCCTAC CGCACCGCAC 901 CGCNNGTGTC CNANTCNGNC NTGNCCGCGA NCCCNTCTTC NNNTNNCAGA ANNTCCCCNA 961 NNCCGNNATC CNCAGCGNGN NNNCNTTTNN CCACGGNTTC NNNNTGNNCN NCNNGCTGGG 1021 NNANNNGNTN NGNNGNNTNN NGNNNNNNNN NN // |
Primer: Venus-C_F2 (Pr0454) Region: insert mid (Akaluc mid) Sequence file: RDB15781_A8Cdj.seq ![]() |
>D04498K3_A8B8_2_Venus-C_F2_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTCTCGNN NTGGNNGAGC TGTACNAGGG NNCCATGGAA 61 GANGCCAAAA ACATTAAGAA GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC 121 GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCGC CATCGCCTTT 181 ACCGACGCAC ATATTCAGGT GGACGTTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG 241 CTGGCAGAAG CTATGAGGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC 301 GAGAATAGCT CGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG 361 GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG 421 CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCGAAAGG TCCTCAACGT GCAAAAGAAG 481 CTACCGATCA TACGAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA 541 AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCAGCT TCAACGAGTA CGACTTCGTG 601 CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGTAGTACA 661 GGATTACCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC 721 CGCGACNNCA TCTTCGGCTA CCAGAACATC CCCGACACCG CTATCCTCAG CGTGGTGCCA 781 TTTCACCACG GCTTCNGCAT GTTCACCACG CTGGGCTANT TGNTCTGCGG CTTTCGGGTC 841 GTGCTCATGT ACNGCTTCGA NGANGANCTA TTCTTGCGCA GCTNGCAAGA NTATAAGANT 901 CAATCTGNCN NGCTGGNGNC CACACNATTT AGCTGCCNNG CTNAGANCNN TNNNNTCGNN 961 NNNNNCNANC NNNNNAGCNN GNNNNANATC GNCNNNNNGN NNGNNNNNNN NNN // |
Primer: Akaluc 576-593 (Pr0539) Region: insert mid (Akaluc mid) Sequence file: RDB15781_A8Cdk.seq ![]() |
>D04498K3_A8B8_2_Akaluc 576-593_D05_11_ABI24.ab1 1 NNNNNNNNNN GNGNNNCCNT NNCGCNCCGC ACCGCTTGTG TCCGATTCNG TCATGCCCGC 61 GACCCCATCT TCGGCTACCA GAACATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT 121 CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG 181 CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA 241 TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TGCCTCGCTA AGAGCACTCT CATCGACAAG 301 TACGACCTAA GCAGCTTGCG CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA 361 GGTGAGGCCG TGGCCAAACG CTTCCGCCTA CCAGGCATCC GCCAGGGCTA TGGCCTGACA 421 GAAACAACCA ACGCCGTCAT GATCACCCCC GAGGGGGACC GTAAGCCTGG CTCAGTAGGC 481 AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTAGACTTGG TCACCGGTAA GACACTGGGT 541 GTGAACCAGC GCGGTGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC 601 AACCCCGAGG CTACGAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC 661 GCCTACTGGG ACGANGACGA NCACTTCTTC ATCGTGGANC GGCTGAAGAG CCTGATCAAA 721 TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGANGGCA TCCTGCTGCA ACACCNCTAC 781 NTCTTCGACG CCNGANNCGC CGGCCTGNCC GANGACGATG NCGGCGANCT GCCCGCCGCA 841 NNCGNCGTGT TNGAACACGG TAAAACCATG ANCGANAAAG ANATCGNGGN NNATGNGGCC 901 AGCCAGGNTN NNACCGNCNN NNNNNCTGNN NNNNNGNNNN NNNNTNNNNG NNGNNNNNCN 961 NANNNGNNNC GNNNNNNNNN N // |
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori, NeoR Sequence file: RDB15781_A8Cdl.seq ![]() |
>D04498K3_A8B8_2_SV40pro_F_F05_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NANTNGTGNN GNNNNTTTTT TGGANGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 661 TGCCCGACGG CGAGGATCTC GTCGTGANCC ATGGCGATGC CTGCTTGCCG AATATCATGG 721 TGGAAAATGN NCGCTTTTCT GGATTCATCG ACTGTGGNCG GCTGGGTGTG GCGGANCGCT 781 ATCANGANAT ANCGTTGGCT ACCCGTGATA TTGCTGAANA NCTTGGNNGG CNAATGGGCT 841 GANNGCTTCC TCGNGCTTTA CNGNATCGCN GCNNCCNATN CGCAGCGCAT CGCNNNCNAT 901 NNCNNTCNNG ANNANTTNTT CTGANCNGNN NTCTNGGGNN NCNAAATGAN NNANCNNNNG 961 ACGCCNNNNC NNNNCNNNNN NNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Iwano, S., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486. [link to RRC of NBRP] |
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original | Miyawaki, A., Modified luciferase. Patent publication WO 2018/038248 A1. [link to RRC of NBRP] |
reference | Nasu, Y., Unnaturally aglow with a bright inner light. Science 359 (6378): 868-869 (2018). PMID 29472468. |
reference | Kuchimaru, T., A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat. Commun. 7: 11856 (2016). PMID 27297211. |
reference | Iwano, S., Development of simple firefly luciferin analogs emitting blue, green, red, and near-infrared biological window light. Tetrahedron 69 (19): 3847-3856 (2013). |
user_report | Oka, T., Ablation of p57+ Quiescent Cancer Stem Cells Suppresses Recurrence after Chemotherapy of Intestinal Tumors. Cancer Res. 83 (9): 1393-1409 (2023). PMID 36880956. [link to RRC of NBRP] |
user_report | Nakashiba, T., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: 10.1038/s41684-023-01238-6. Epub ahead of print. PMID 37679611. [link to RRC of NBRP] |
user_report | Amadeo, F., Firefly luciferase offers superior performance to AkaLuc for tracking the fate of administered cell therapies. Eur. J. Nucl. Med. Mol. Imaging 49 (3): 796-808 (2022). PMID 34313817. [link to RRC of NBRP] |
user_report | Sugimoto, N., Production and nonclinical evaluation of an autologous iPSC-derived platelet product for the iPLAT1 clinical trial. Blood Adv. 2022 Sep 23:bloodadvances.2022008512. PMID 36149941. [link to RRC of NBRP] |
user_report | Shono, Y., Protection of liver sinusoids by intravenous administration of human Muse cells in a rat extra-small partial liver transplantation model. Am. J. Transplant. 21 (6): 2025-2039 (2021). PMID 33350582. [link to RRC of NBRP] |
user_report | Kayamori, K., DHODH inhibition synergizes with DNA-demethylating agents in the treatment of myelodysplastic syndromes. Blood Adv. 5 (2): 438-450 (2021). PMID 33496740. [link to RRC of NBRP] |
user_report | Gaspar, N., Evaluation of NanoLuc substrates for bioluminescence imaging of transferred cells in mice. J. Photochem. Photobiol. B. 216: 112128 (2021). PMID 33529963. [link to RRC of NBRP] |
user_report | Zambito, G., Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging. Mol. Imaging Biol. 22 (6): 1523-1531 (2020). PMID 32926287. [link to RRC of NBRP] |
user_report | Hikita, T., In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter. Sci. Rep. 10 (1): 16616 (2020). PMID 33024173. [link to RRC of NBRP] |
user_report | Zhong, C., Efficacy of the novel tubulin polymerization inhibitor PTC-028 for myelodysplastic syndrome. Cancer Sci. 111 (12): 4336-4347 (2020). PMID 33037737. [link to RRC of NBRP] |
user_report | Bozec, D., Akaluc bioluminescence offers superior sensitivity to track in vivo glioma expansion. Neurooncol. Adv. 2 (1): vdaa134 (2020). PMID 33241215. [link to RRC of NBRP] |
2023.09.13
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