Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Kaede/pRSETB

Plasmid clone of fluorescent protein Kaede which shows UV-induced green-to-red photoconversion.

Catalog number RDB15231
Resource name Kaede/pRSETB
Clone info. Plasmid clone of fluorescent protein Kaede which shows UV-induced green-to-red photoconversion. Bacterial expressin vector.
Vector backbone pRSETB (Plasmid)
Selectable markers Amp^r
Gene/insert name Trachyphyllia geoffroyi GFP
Reference of insert sequence AB085641 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank
External Database
Trachyphyllia geoffroyi GFP
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. (2002) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. (2002) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15231 Kaede/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The Kaede/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15231).

            Reference section:

            Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H., Miyawaki, A., An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PMID 12271129. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15231_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp (Pr0261)
            Region: RBS,6XHis,T7 tag,Xpress tag,insert5'
            Sequence file: RDB15231_A7H9a.seq check
            >D04498D7-1_A7H9_T7_20bp_E01_13_ABI24.ab1
                1 GAAAATTCGT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TATGCGGGGT
               61 TCTCATCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG
              121 GATCTGTACG ACGATGACGA TAAGGATCCG ATGAGTCTGA TTAAACCAGA AATGAAGATC
              181 AAGCTGCTTA TGGAAGGCAA TGTAAACGGG CACCAGTTTG TTATTGAGGG AGATGGAAAA
              241 GGCCATCCTT TTGAGGGAAA ACAGAGTATG GACCTTGTAG TCAAAGAAGG CGCACCTCTC
              301 CCTTTTGCCT ACGATATCTT GACAACAGCA TTCCATTATG GTAACAGGGT TTTTGCTAAA
              361 TACCCAGACC ATATACCAGA CTACTTCAAG CAGTCGTTTC CCAAAGGGTT TTCTTGGGAG
              421 CGAAGCCTGA TGTTCGAGGA CGGGGGCGTT TGCATCGCTA CAAATGACAT AACACTGAAA
              481 GGAGACACTT TTTTTAACAA AGTTCGATTT GATGGCGTAA ACTTTCCCCC AAATGGTCCT
              541 GTTATGCAGA AGAAGACTCT GAAATGGGAG GCATCCACTG AGAAAATGTA TTTGCGTGAT
              601 GGAGTGTTGA CGGGCGATAT TACCATGGCT CTGCTGCTTA AAGGAGATGT CCATTACCGA
              661 TGTGACTTCA GAACTACTTA CAAATCTAGG CAGGAGGGTG TCAAGTTGCC AGGATATCAC
              721 TTTGTCGATC ACTGCATCAG CATATTGAGG CATGACAAAG ACTACAACGA GGTTAAGCTG
              781 TATGAGCATG CTGTTGCCAA TTCTGGATGC CGGACAACGG TCAAGTTAGG ATTTCGAGCT
              841 TGATCCGCTG CTAAACAAGC CCGAAGGAAG CTGAGTGGCT GCTGCCACCC GCTGGAACCA
              901 TAAACTAGAC TAAACCCTTG GGCCTCTAAC GGTCTTGAGG GTCTTTGGCT GAGGAGGAAC
              961 TAATCGGATC TGGCTATAAC GAAGGCCGAC CGATAGCTCT CCAACATGTG GCACTGTAAT
            1021 GCGCAATAGG ACCGCCCGTG AT
            //
            Primer: f1ori_R3 (Pr0142)
            Region: T7 ter,insert3'
            Sequence file: RDB15231_A7H9b.seq check
            >D04498D7-1_A7H9_f1ori_R3_F01_16_ABI24.ab1
                1 GCATGCATCA GCTGCGCACT GTTGGGAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
               61 CAGATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC
              121 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
              181 CTTTGTTAGC AGCCGGATCA AGCTTCGAAT TCTTACTTGA CGTTGTCCGG CAATCCAGAA
              241 TGGGCAACAG CATGCTCATA CAGCTTAACC TCGTTGTAGT CTTTGTCATG CCTCAATATG
              301 CTGATGCAGT GATCGACAAA GTGATATCCT GGCAACTTGA CACCCTCCTG CCTAGATTTG
              361 TAAGTAGTTC TGAAGTCACA TCGGTAATGG ACATCTCCTT TAAGCAGCAG AGCCATGGTA
              421 ATATCGCCCG TCAACACTCC ATCACGCAAA TACATTTTCT CAGTGGATGC CTCCCATTTC
              481 AGAGTCTTCT TCTGCATAAC AGGACCATTT GGGGGAAAGT TTACGCCATC AAATCGAACT
              541 TTGTTAAAAA AAGTGTCTCC TTTCAGTGTT ATGTCATTTG TAGCGATGCA AACGCCCCCG
              601 TCCTCGAACA TCAGGCTTCG CTCCCAAGAA AACCCTTTGG GAAACGACTG CTTGAAGTAG
              661 TCTGGTATAT GGTCTGGGTA TTTAGCAAAA ACCCTGTTAC CATAATGGAA TGCTGTTGTC
              721 AAGATATCGT AGGCAAAAGG GAGAGGTGCG CCTTCTTTGA CTACAAGGTC CATACTCTGT
              781 TTTCCCTCAA AAGGATGGCC TTTTCCATCT CCCTCAATAA CAAACTGGTG CCCGTTTACA
              841 TTGCCTTCCA TAAGCAGCTT GATCTTCATT TCTGGTTTAA TCAGACTCAT CGGATCCTTA
              901 TCGTCATCGT CGTACAGATC CCGACCCATT TGCTGTCCAC CAGTCATGCT AGCCATACCA
              961 TGATGATGAT GATGATGAGA ACCCCGCATA TGTATATCTC CTTCTTAAAG GTTAAACAAA
             1021 ATTTATTTCT AGAGGGGAAC CCGGTGTGGT CTCCCTATAG TGAGTTCGTA ATAAATTCGC
             1081 GGGATCGAAG ATTCTGCATA ATGATTCCGT CCACGCGCGG GGATAGCCGG TTTGGCGGAA
            1141 T
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ando, R., An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PMID 12271129. [link to RRC of NBRP]

            2023.08.18

            GNP_filter3_RDBDEP_html_230702.pl