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Kaede/pRSETB

Plasmid clone of fluorescent protein Kaede which shows UV-induced green-to-red photoconversion.

Catalog number RDB15231
Resource name Kaede/pRSETB
Clone info. Plasmid clone of fluorescent protein Kaede which shows UV-induced green-to-red photoconversion. Bacterial expressin vector.
Vector backbone pRSETB (Plasmid)
Selectable markers Amp^r
Gene/insert name Trachyphyllia geoffroyi GFP
Reference of nucleotide sequence AB085641 (DDBJ accession number)
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. (2002) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15231 Kaede/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Ando, R., An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PMID 12271129.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15231_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15231_A7H9a.seq
>D04498D7-1_A7H9_T7_20bp_E01_13_ABI24.ab1
    1 GAAAATTCGT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TATGCGGGGT
   61 TCTCATCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG
  121 GATCTGTACG ACGATGACGA TAAGGATCCG ATGAGTCTGA TTAAACCAGA AATGAAGATC
  181 AAGCTGCTTA TGGAAGGCAA TGTAAACGGG CACCAGTTTG TTATTGAGGG AGATGGAAAA
  241 GGCCATCCTT TTGAGGGAAA ACAGAGTATG GACCTTGTAG TCAAAGAAGG CGCACCTCTC
  301 CCTTTTGCCT ACGATATCTT GACAACAGCA TTCCATTATG GTAACAGGGT TTTTGCTAAA
  361 TACCCAGACC ATATACCAGA CTACTTCAAG CAGTCGTTTC CCAAAGGGTT TTCTTGGGAG
  421 CGAAGCCTGA TGTTCGAGGA CGGGGGCGTT TGCATCGCTA CAAATGACAT AACACTGAAA
  481 GGAGACACTT TTTTTAACAA AGTTCGATTT GATGGCGTAA ACTTTCCCCC AAATGGTCCT
  541 GTTATGCAGA AGAAGACTCT GAAATGGGAG GCATCCACTG AGAAAATGTA TTTGCGTGAT
  601 GGAGTGTTGA CGGGCGATAT TACCATGGCT CTGCTGCTTA AAGGAGATGT CCATTACCGA
  661 TGTGACTTCA GAACTACTTA CAAATCTAGG CAGGAGGGTG TCAAGTTGCC AGGATATCAC
  721 TTTGTCGATC ACTGCATCAG CATATTGAGG CATGACAAAG ACTACAACGA GGTTAAGCTG
  781 TATGAGCATG CTGTTGCCAA TTCTGGATGC CGGACAACGG TCAAGTTAGG ATTTCGAGCT
  841 TGATCCGCTG CTAAACAAGC CCGAAGGAAG CTGAGTGGCT GCTGCCACCC GCTGGAACCA
  901 TAAACTAGAC TAAACCCTTG GGCCTCTAAC GGTCTTGAGG GTCTTTGGCT GAGGAGGAAC
  961 TAATCGGATC TGGCTATAAC GAAGGCCGAC CGATAGCTCT CCAACATGTG GCACTGTAAT
1021 GCGCAATAGG ACCGCCCGTG AT
//
Primer: f1ori_R3 RDB15231_A7H9b.seq
>D04498D7-1_A7H9_f1ori_R3_F01_16_ABI24.ab1
    1 GCATGCATCA GCTGCGCACT GTTGGGAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
   61 CAGATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC
  121 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  181 CTTTGTTAGC AGCCGGATCA AGCTTCGAAT TCTTACTTGA CGTTGTCCGG CAATCCAGAA
  241 TGGGCAACAG CATGCTCATA CAGCTTAACC TCGTTGTAGT CTTTGTCATG CCTCAATATG
  301 CTGATGCAGT GATCGACAAA GTGATATCCT GGCAACTTGA CACCCTCCTG CCTAGATTTG
  361 TAAGTAGTTC TGAAGTCACA TCGGTAATGG ACATCTCCTT TAAGCAGCAG AGCCATGGTA
  421 ATATCGCCCG TCAACACTCC ATCACGCAAA TACATTTTCT CAGTGGATGC CTCCCATTTC
  481 AGAGTCTTCT TCTGCATAAC AGGACCATTT GGGGGAAAGT TTACGCCATC AAATCGAACT
  541 TTGTTAAAAA AAGTGTCTCC TTTCAGTGTT ATGTCATTTG TAGCGATGCA AACGCCCCCG
  601 TCCTCGAACA TCAGGCTTCG CTCCCAAGAA AACCCTTTGG GAAACGACTG CTTGAAGTAG
  661 TCTGGTATAT GGTCTGGGTA TTTAGCAAAA ACCCTGTTAC CATAATGGAA TGCTGTTGTC
  721 AAGATATCGT AGGCAAAAGG GAGAGGTGCG CCTTCTTTGA CTACAAGGTC CATACTCTGT
  781 TTTCCCTCAA AAGGATGGCC TTTTCCATCT CCCTCAATAA CAAACTGGTG CCCGTTTACA
  841 TTGCCTTCCA TAAGCAGCTT GATCTTCATT TCTGGTTTAA TCAGACTCAT CGGATCCTTA
  901 TCGTCATCGT CGTACAGATC CCGACCCATT TGCTGTCCAC CAGTCATGCT AGCCATACCA
  961 TGATGATGAT GATGATGAGA ACCCCGCATA TGTATATCTC CTTCTTAAAG GTTAAACAAA
 1021 ATTTATTTCT AGAGGGGAAC CCGGTGTGGT CTCCCTATAG TGAGTTCGTA ATAAATTCGC
 1081 GGGATCGAAG ATTCTGCATA ATGATTCCGT CCACGCGCGG GGATAGCCGG TTTGGCGGAA
1141 T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ando, R., An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PMID 12271129.

2019.02.26

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