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NPY-Venus/pEF-BOS

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Catalog number RDB15119
Resource name NPY-Venus/pEF-BOS
Clone info. Venus fluorescent protein targeting localization in secretory dense-core granules of neurites (tagged with neuropeptide Y (NPY)). Mammalian expression.
Vector backbone pEF-BOS (plasmid)
Selectable markers Amp^r
Gene/insert name Human NPY cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank human NPY (NCBI Gene 4852) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15119 NPY-Venus/pEF-BOS DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15119_A8A9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB15119_A8A9a.seq check
>D04498A4_A7H9_1_EF1a-C_F_H01_22_ABI24.ab1
    1 GGGGTAATCG TTGGGAATTT GCCCTTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGGCC
   61 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG
  121 CGGCCGCAGC CACCATGCTA GGTAACAAGC GACTGGGGCT GTCCGGACTG ACCCTCGCCC
  181 TGTCCCTGCT CGTGTGCCTG GGTGCGCTGG CCGAGGCGTA CCCCTCCAAG CCGGACAACC
  241 CGGGCGAGGA CGCACCAGCG GAGGACATGG CCAGATACTA CTCGGCGCTG CGACACTACA
  301 TCAACCTCAT CACCAGGCAG AGATATGGAA AACGATCCAG CCCAGAGACA CTGATTTCAG
  361 ACCTCTTGAT GAGAGAAAGC ACAGAAAATG TTCCCAGAAC TCGGCTTGAA GACCCTGCAA
  421 TGTGGCAATT CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG
  481 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG
  541 ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC
  601 CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG
  661 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCCAGGGA
  721 GCGCACCATC TTCTTCAAGG ACGACGGGCA AACTACAAGA CCCGCGCCGA GGTGAAGTTC
  781 GAGGGCGACA ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTTC AAGGAGGGAC
  841 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCACC
  901 GCCGACAAGC AGAAGAAACG GCATCAAGGG CCAACTTCAA GATCCGCCAC AAACATCGAG
  961 GACGGCGGGC GTGCAGGCTC GCCGACCACT AACCAGCAGA ACCACCCCCC CAATCGGCGA
 1021 CGGCCCCCGT TGCTGCTGCC CGACAACCCA CTACCCTGGA GCCTTACCAG TTCCGCCCTG
 1081 AAGCAAAGAA CCCCCAACGA AGAAAGCGCG GATCCACATG GGTCCTGGCT GGGAGTTTCG
 1141 TGTGACCCGG CCGGCCGGAA ATCCACTCTC GGCATGGAAC GGAGCTGGTA TCAAAGATTA
1201 AAACTTATGA TGGTGAAAAA TTC
//
Primer: hGCSF_R
Sequence file: RDB15119_A8A9b.seq check
>D04498A4_A7H9_1_hGCSF_R_A02_02_ABI24.ab1
    1 CTGGGAACAA TGGATCTCTC AAGGGTCCAG GTGGGGACCC TCACTCTAAG AGCGGCCGCG
   61 AATTCACTAG TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
  181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  241 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG
  301 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG
  361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
  421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
  541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG
  601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT CAGCTTCAGG
  661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
  721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
  781 TTGCTCACCA TGAATTGCCA CATTGCAGGG TCTTCAAGCC GAGTTCTGGG AACATTTTCT
  841 GTGCTTTCTC TCATCAAGAG GTCTGAAATC AGTGTCTCTG GGCTGGATCG TTTTTCCATA
  901 TCTCTGCCTG GTGATGAGGT TGATGTAGTG TCGCAGCGCC GAGTAGTATC TGGCCATGTC
  961 CTCCGCTGGG TGCGTCCTCG CCCGGGGTTG TCCGGGCTTG GAGGGGTACG CCTTCGGCCA
 1021 GCGCATCCAG GCACACGGAG CAGGACAGGG CGAGGGGTTC AGTCGACGAG CCCCAGTCGC
 1081 TTGTTACCTT AGCATGTGAC TGGCGGCCGC CTTCTAAAGA ATCCTCACGA CACCTGAATG
1141 AAGAAAAAAA CCTTGGAACT CACTGTCCTG TGAAGGGGCC T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.
reference Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345.
reference Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140.
reference Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

2020.12.17

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