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NPY-Venus/pEF-BOS

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Catalog number RDB15119
Resource name NPY-Venus/pEF-BOS
Clone info. Venus fluorescent protein targeting localization in secretory dense-core granules of neurites (tagged with neuropeptide Y (NPY)). Mammalian expression.
Vector backbone pEF-BOS (plasmid)
Selectable markers Amp^r
Gene/insert name Human NPY cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human NPY Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15119 NPY-Venus/pEF-BOS DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15119_A8A9p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15119_A8A9a.seq check
          >D04498A4_A7H9_1_EF1a-C_F_H01_22_ABI24.ab1
              1 GGGGTAATCG TTGGGAATTT GCCCTTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGGCC
             61 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG
            121 CGGCCGCAGC CACCATGCTA GGTAACAAGC GACTGGGGCT GTCCGGACTG ACCCTCGCCC
            181 TGTCCCTGCT CGTGTGCCTG GGTGCGCTGG CCGAGGCGTA CCCCTCCAAG CCGGACAACC
            241 CGGGCGAGGA CGCACCAGCG GAGGACATGG CCAGATACTA CTCGGCGCTG CGACACTACA
            301 TCAACCTCAT CACCAGGCAG AGATATGGAA AACGATCCAG CCCAGAGACA CTGATTTCAG
            361 ACCTCTTGAT GAGAGAAAGC ACAGAAAATG TTCCCAGAAC TCGGCTTGAA GACCCTGCAA
            421 TGTGGCAATT CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG
            481 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG
            541 ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC
            601 CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG
            661 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCCAGGGA
            721 GCGCACCATC TTCTTCAAGG ACGACGGGCA AACTACAAGA CCCGCGCCGA GGTGAAGTTC
            781 GAGGGCGACA ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTTC AAGGAGGGAC
            841 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCACC
            901 GCCGACAAGC AGAAGAAACG GCATCAAGGG CCAACTTCAA GATCCGCCAC AAACATCGAG
            961 GACGGCGGGC GTGCAGGCTC GCCGACCACT AACCAGCAGA ACCACCCCCC CAATCGGCGA
           1021 CGGCCCCCGT TGCTGCTGCC CGACAACCCA CTACCCTGGA GCCTTACCAG TTCCGCCCTG
           1081 AAGCAAAGAA CCCCCAACGA AGAAAGCGCG GATCCACATG GGTCCTGGCT GGGAGTTTCG
           1141 TGTGACCCGG CCGGCCGGAA ATCCACTCTC GGCATGGAAC GGAGCTGGTA TCAAAGATTA
          1201 AAACTTATGA TGGTGAAAAA TTC
          //
          Primer: hGCSF_R
          Sequence file: RDB15119_A8A9b.seq check
          >D04498A4_A7H9_1_hGCSF_R_A02_02_ABI24.ab1
              1 CTGGGAACAA TGGATCTCTC AAGGGTCCAG GTGGGGACCC TCACTCTAAG AGCGGCCGCG
             61 AATTCACTAG TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
            121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
            181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
            241 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG
            301 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG
            361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
            421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
            481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
            541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG
            601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT CAGCTTCAGG
            661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
            721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
            781 TTGCTCACCA TGAATTGCCA CATTGCAGGG TCTTCAAGCC GAGTTCTGGG AACATTTTCT
            841 GTGCTTTCTC TCATCAAGAG GTCTGAAATC AGTGTCTCTG GGCTGGATCG TTTTTCCATA
            901 TCTCTGCCTG GTGATGAGGT TGATGTAGTG TCGCAGCGCC GAGTAGTATC TGGCCATGTC
            961 CTCCGCTGGG TGCGTCCTCG CCCGGGGTTG TCCGGGCTTG GAGGGGTACG CCTTCGGCCA
           1021 GCGCATCCAG GCACACGGAG CAGGACAGGG CGAGGGGTTC AGTCGACGAG CCCCAGTCGC
           1081 TTGTTACCTT AGCATGTGAC TGGCGGCCGC CTTCTAAAGA ATCCTCACGA CACCTGAATG
          1141 AAGAAAAAAA CCTTGGAACT CACTGTCCTG TGAAGGGGCC T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.
          reference Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345.
          reference Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140.
          reference Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl