RDB15119 - NPY-Venus/pEF-BOS

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NPY-Venus/pEF-BOS (#RDB15119)

Venus fluorescent protein targeting localization in neurites. Mammalian expression.

Clone info.	Venus fluorescent protein targeting localization in secretory dense-core granules of neurites (tagged with neuropeptide Y (NPY)). Mammalian expression.
Vector backbone	pEF-BOS (plasmid)
Selectable markers	Amp^r
Growth conditions	LB + Amp, 37oC
Gene/insert name	Human NPY cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002). Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002). 付加的使用条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15119	NPY-Venus/pEF-BOS	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The NPY-Venus/pEF-BOS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15119).

Reference section:

Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K., Miyawaki, A., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15119_A8A9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F () Sequence file: RDB15119_A8A9a.seq
>D04498A4_A7H9_1_EF1a-C_F_H01_22_ABI24.ab1 1 GGGGTAATCG TTGGGAATTT GCCCTTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGGCC 61 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG 121 CGGCCGCAGC CACCATGCTA GGTAACAAGC GACTGGGGCT GTCCGGACTG ACCCTCGCCC 181 TGTCCCTGCT CGTGTGCCTG GGTGCGCTGG CCGAGGCGTA CCCCTCCAAG CCGGACAACC 241 CGGGCGAGGA CGCACCAGCG GAGGACATGG CCAGATACTA CTCGGCGCTG CGACACTACA 301 TCAACCTCAT CACCAGGCAG AGATATGGAA AACGATCCAG CCCAGAGACA CTGATTTCAG 361 ACCTCTTGAT GAGAGAAAGC ACAGAAAATG TTCCCAGAAC TCGGCTTGAA GACCCTGCAA 421 TGTGGCAATT CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG 481 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG 541 ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC 601 CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG 661 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCCAGGGA 721 GCGCACCATC TTCTTCAAGG ACGACGGGCA AACTACAAGA CCCGCGCCGA GGTGAAGTTC 781 GAGGGCGACA ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTTC AAGGAGGGAC 841 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCACC 901 GCCGACAAGC AGAAGAAACG GCATCAAGGG CCAACTTCAA GATCCGCCAC AAACATCGAG 961 GACGGCGGGC GTGCAGGCTC GCCGACCACT AACCAGCAGA ACCACCCCCC CAATCGGCGA 1021 CGGCCCCCGT TGCTGCTGCC CGACAACCCA CTACCCTGGA GCCTTACCAG TTCCGCCCTG 1081 AAGCAAAGAA CCCCCAACGA AGAAAGCGCG GATCCACATG GGTCCTGGCT GGGAGTTTCG 1141 TGTGACCCGG CCGGCCGGAA ATCCACTCTC GGCATGGAAC GGAGCTGGTA TCAAAGATTA 1201 AAACTTATGA TGGTGAAAAA TTC //
Primer: hGCSF_R () Sequence file: RDB15119_A8A9b.seq
>D04498A4_A7H9_1_hGCSF_R_A02_02_ABI24.ab1 1 CTGGGAACAA TGGATCTCTC AAGGGTCCAG GTGGGGACCC TCACTCTAAG AGCGGCCGCG 61 AATTCACTAG TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG 121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG 181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC 241 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG 301 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG 361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG 421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG 481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG 541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG 601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT CAGCTTCAGG 661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG 721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC 781 TTGCTCACCA TGAATTGCCA CATTGCAGGG TCTTCAAGCC GAGTTCTGGG AACATTTTCT 841 GTGCTTTCTC TCATCAAGAG GTCTGAAATC AGTGTCTCTG GGCTGGATCG TTTTTCCATA 901 TCTCTGCCTG GTGATGAGGT TGATGTAGTG TCGCAGCGCC GAGTAGTATC TGGCCATGTC 961 CTCCGCTGGG TGCGTCCTCG CCCGGGGTTG TCCGGGCTTG GAGGGGTACG CCTTCGGCCA 1021 GCGCATCCAG GCACACGGAG CAGGACAGGG CGAGGGGTTC AGTCGACGAG CCCCAGTCGC 1081 TTGTTACCTT AGCATGTGAC TGGCGGCCGC CTTCTAAAGA ATCCTCACGA CACCTGAATG 1141 AAGAAAAAAA CCTTGGAACT CACTGTCCTG TGAAGGGGCC T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]
reference	Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345.
reference	Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140.
reference	Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]