Resource data sheet
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mCherry-hGeminin(1/110) / pCSII-EF

Lentiviral expression vector of S/G2/M-R(N).

Catalog number RDB15269
Resource name mCherry-hGeminin(1/110) / pCSII-EF
Clone info. Lentiviral expression vector of S/G2/M-R(N). Fluorescence was detected in the nucleus.
For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008).
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells)
Gene/insert name human Geminin cDNA Discosoma sp. RFP cDNA
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15269_A7H3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15269_A7H3a.seq
>D04498H9-1_A7H3_EF1a-C_F_C01_07_ABI24.ab1
    1 TCATTGGAAT TGGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG
   61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC TCGAGAATTC
  121 GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC
  181 TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC
  241 GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC
  301 CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG
  361 AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG
  421 GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG
  481 CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC
  541 CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG
  601 GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC
  661 GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC
  721 AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG
  781 TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGGGATAT
  841 CCATCACACT GGCGGCCGCT CGAGATGAAT CCCAGTATGA AGCAGAAACA AGAAGAAATC
  901 AAAGAGAATA TAAAGAATAG TTCTGTCCCA AGAAGAACTC TGAAGATGAT TCAGCCTTCT
  961 GCATCTGGGA TCTCTTGTTG GAAGAGAAAA TGAGCTGTCC GCAGCTTGTC CAAAAGGAAA
 1021 CATCGGAATG ACCACTTTAA CATCTACAAC TTCCAGCCCT GGGGGTATGT CCCAGAATCT
 1081 AGTGAAAAAT AAAAAATCTG AGAGTCACCC AGGGAGTCAT TGGATCCTAA TGAATCAGAA
1141 ATCAATCTCT CAGTATTGGA AAGGAAGTGG CAGAAAAAAA ACC
//
Primer: PRE_rev RDB15269_A7H3b.seq
>D04498H9-1_A7H3_PRE_rev_C02_08_ABI24.ab1
    1 ACGTTATCAC ATAGCGTAAA GGAGCAACAT AGTTAAGAAT ACCAGTCAAT CTTTCACAAA
   61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGATTA
  121 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT
  181 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC
  241 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT
  301 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG
  361 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC
  421 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC
  481 CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT CGTACTGTTC
  541 CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC TTGATGTTGA CGTTGTAGGC
  601 GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC TTGACCTCAG CGTCGTAGTG
  661 GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG
  721 GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC
  781 GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA
  841 GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT
  901 GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA
  961 GGCCTTGGAG CCGTACATGA ACTGAGGGAC AGGATGTCCC AGGCGAAAGG GCAGGGGCCA
 1021 CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GGTGCCCCTC GTAGGGGCGC CCTCGCCCTC
 1081 GCCCTCGATC TCGAACTCGT ATCGTTCACG GAGCCCTCCA TGGTGCAACT GGAGCGCAAT
1141 GGACTCATGG ATGATGGGCC ATGTTTATCC TTCCCTCGGC CCCTGTGCTT CT
//
Primer: CMV-Forward RDB15269_A7H3c.seq
>D04498H9-1_A7H3_CMV-Forward_C03_09_ABI24.ab1
    1 TTGTCAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC
  121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC
  181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT
  241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA
  301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA
  361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA
  421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG
  481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA
  541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT
  601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA
  661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC
  721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGGGCCGCT
  781 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA
  841 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT
  901 GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC CTTGGGTTCT TGGAGCAGCA
  961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTTATTGTC
 1021 TGGTATAGTG CAGCAGCAGA ACAAATTTGC TGAGGGCTAT TGAGCGCAAC AGCAATCTGG
 1081 TGGCAACTCA ACAGGTCTGG GGCATCAAGC AGCTTCAAGG CAGATTCCTG ACTGATGAAA
1141 GAAATACCTT AAAAGGAATT C
//
Primer: BGH_rev2 RDB15269_A7H3d.seq
>D04498H9-1_A7H3_BGH_rev2_C04_07_ABI24.ab1
    1 CAGGTCGGGT CAGTAGCACG GGGGAGGGGC AACAAGCAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTGCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
  121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
  181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
  241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
  301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
  361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
  421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
  481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
  541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
  601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
  661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
  721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
  781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
  841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
  901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
  961 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT TCCCCGACAA CACCACGGGA ATTTGTCAGT
 1021 GCCCAACAGC CCGAGCCCCT GTCCAGCAGC GGGCAAGGCA GGCGGCGATG AGTTCCGCCG
 1081 TGCATTAGGA GGGGGAAGCG AAGTCCGGAA AGAGCTGACA GTGTGGCATG CCTCACCAGC
1141 TGGGGGGTTG GGCGGTCCAG GCC
//
Primer: SV40pro_F RDB15269_A7H3e.seq
>D04498H9-1_A7H3_SV40pro_F_C05_08_ABI24.ab1
    1 CCTGGCCCCG TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
  961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
 1021 TAATGAATCG GCCCAACGCG CGGGGGAGAG GCGGTTTGCG TATTTGGGGC GGCTTCTTCG
 1081 CTTCTCGCTT CACTTGACTC GCTGCGCTCG ATCGGTTTCG GGCTGCGCCA GCCGGTAATC
1141 AGCTTCACAT GAAGACGGTT AGATACCTGC TGAATCCCTC ACCAG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.

2017.11.22

GNP_filter3_RDBDEP_html_171106.pl