Resource data sheet
DNA Bank Top

pBS Coupler1/td8ox2StayGold (#RDB20227)

Plasmid vector of td8ox2StayGold for fusion to the C-terminus of a target molecule (X) through a Coupler linker: (GGGGS)x3.


Drawn by SnapGene® software
Sequence (full) RDB20227hts01.seq
Publication Ando, R., Nat. Methods 21 (4): 648-656 (2024). [link to RRC of NBRP]
Test sheet RDB20227_B3Lsp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. For fusion of td8ox2StayGold to the C-terminus of a target molecule (X) through a Coupler linker: (GGGGS)x3. td8ox2StayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, oxStayGold(Y169F), PT, a 21-residue linker, n1, and oxStayGold(Y169F). n1 is the appendage that facilitates fusions to the N-terminus of oxStayGold(Y169F). PT is the appendage that facilitates fusions to the C-terminus of oxStayGold(Y169F). td8ox2StayGold(c4) encoded by this plasmid DNA follows a Coupler linker: (GGGGS)×3. See, Supp. Fig.14 of Ando, R. et al. Nat. Methods, 2023. https://doi.org/10.1038/s41592-023-02085-6 for more information.
Vector backbone pBS Coupler1 (RDB15126) (plasmid)
Gene/insert name Cytaeis uchidae GFP (StayGold) cDNA.
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando, R. et al. Nat. Methods 21 (4): 648-656 (2024).
4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2.A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando, R. et al. Nat. Methods 21 (4): 648-656 (2024).
4.The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
5.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN Innovation Co., Ltd. and must obtain a prior written consent from the DEPOSITOR.
備考
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20227 pBS Coupler1/td8ox2StayGold DNA solution

How to cite this biological resource

Materials & Methods section:

The pBS Coupler1/td8ox2StayGold was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20227).

Reference section:

Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [link to RRC of NBRP]