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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)2.2/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15448
Resource name tFucci(CA)2.2/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of insert sequence LC212778 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Human Geminin Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
         
        Sequence (full) RDB15448zzt01.seq compiled by us
        Lentiviral plasmid list. [link]

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Ordering instruction.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Remarks Remember that you will be working with samples containing infectious virus.
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
        3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
        付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
        提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
        提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15448 tFucci(CA)2.2/pCSII-EF DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15448_A7Llp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EF1a-C_F
        Sequence file: RDB15448_A7Lla.seq check
        >D05018D1-1_A7Ll_EF1a-C_F_A02_02_ABI24.ab1
            1 CATCATGCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG
           61 TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT TCTGCAGCGG
          121 CCGCGGATCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT
          181 CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG
          241 CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG
          301 TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC
          361 CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT
          421 CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC
          481 CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC
          541 CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA
          601 CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG
          661 CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG
          721 CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT
          781 GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA
          841 GGGATATCCA TCACACTGGC GGCCGCTCGA GATGGAGCAG CGCCGCGTCA CCGACTTCTT
          901 CGCGCGCCGC CGCCCCGGGC CCCCCCGCAT CGCGCCGCCC AAGCTGGCCT GCCGCACCCC
          961 CAGCCCCGCC AGGCCGCACT CGCGCCCCGG CCTCCGCTAC AGTGCCAGCG CCAAGCGCGC
         1021 CGCCGGCGGC GGCCCCCGAC GCGACAGCCA AGCAACGACC GGGCGATCGC CGCTGTCGAT
        1081 GACGAAGGAT TCCAGGCTCC AGTTATCG
        //
        Primer: mCherry-C_F
        Sequence file: RDB15448_A7Llb.seq check
        >D05018D1-1_A7Ll_mCherry-C_F_B02_05_ABI24.ab1
            1 TACCAGTAGC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
           61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
          121 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGATGG
          181 AGCAGCGCCG CGTCACCGAC TTCTTCGCGC GCCGCCGCCC CGGGCCCCCC CGCATCGCGC
          241 CGCCCAAGCT GGCCTGCCGC ACCCCCAGCC CCGCCAGGCC CGCACTCCGC GCCCCGGCCT
          301 CCGCTACCAG TGGCAGCCGC AAGCGCGCCC GCCCGCCCGC CGCCCCCGGA CGCGACCAGG
          361 CCAGGCCACC GGCCCGCGCG GCCGCGCGGC TGTCGGTGGA CGAGGTTTCC AGCCCCAGTA
          421 CCCCCGAGGC CCCAGACATC CCAGCCTGCC CTTCTCCGGG CCAGAAGATA AAGAAAACCG
          481 GTCGCGCCAA GCGCGGCAGC GGCGCCACCA ACTTCAGCCT GCTGAAGCAG GCCGGCGACG
          541 TGGAGGAGAA CCCCGGCCCC CGCGAATTCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
          601 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
          661 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
          721 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGAGCTGG GGCGTGCAGT
          781 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
          841 AAGGCTACGT CCAGGAGCGT ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
          901 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
          961 TCAAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT CAGCGACACG
         1021 TCTATATCAC CGCCCGACAG CAGAAGAACG GGCATCAAGG CCAACTTTCA AGATCCGCCA
         1081 CACATCGAGG GACGGCGGCG GTGCAGCTCG GCGGACACTA ACAAGCCAGA CCACCCCCAT
        1141 TCGGGCGAAC GGGCCCCCGG AGTGCCTTGA CG
        //
        Primer: PRE_rev
        Sequence file: RDB15448_A7Llc.seq check
        >D05018D1-1_A7Ll_PRE_rev_C02_08_ABI24.ab1
            1 ACGATCAATA GCGTAAGGAG CAACATAGTT AAGAATACCA GTCAATCTTT CACAAATTTT
           61 GTAATCCAGA GGTTGATTAT CGATAAGCTT GATCCCTCGA TGTTAACTCT AGATTACAGC
          121 GCCTTTCTCC GTTTTTCTGC CACTTCCTTC CAATACTGAG AGGATGGATT TTCTTTAATC
          181 ATAAGATCAA ATGACTCCTG GGTGACTCCT CCAAGATTTT TATTTTCACT AGATTCTGGG
          241 ACAATAACCC CAGGGCTGGA AGTTGTAGAT GTTAAGTGGT CATTCCGATG TTTCCTTTTG
          301 GACAAGCCTG CGGACAGCTC ATTTTCTCTT CCAACAAGAG ATCCAGATGC AGAAGGCTGA
          361 ATCATCTTCA GAGTTCTTCT TGGGACAGAA CTATTCTTTA TATTCTCTTT GATTTCTTCT
          421 TGTTTCTGCT TCATACTGGG ATTCATCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG
          481 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
          541 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCTTGGACT GGGTGCTCAG GTAGTGGTTG
          601 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
          661 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC
          721 TGCTTGTCGG CGGTGATATA GACGTTGTCG CTGATGTAGT TGTACTCCAG CTTGTGCCCC
          781 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
          841 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
          901 GTACGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
          961 TGGTCGGGGT AGCGGGCGAA GCACTGCACG CCCCAGCTCA GGTGGTCACG AGGGTGGGCC
         1021 AGGGCACGGG CAGCTTTGCC GGTGGTGCAG ATGACTTCAG GTCAGCTTGC CGTAGGTGGC
         1081 ATCGTCACGC CCTCGCGGAA CACGCTGAAC TGTGTCGATT AACGTTCGGC GTCAGCTCGG
        1141 AACCGAGGAA ATGGGGCATC TA
        //
        Primer: CMV-Forward
        Sequence file: RDB15448_A7Lld.seq check
        >D05018D1-1_A7Ll_CMV-Forward_D02_11_ABI24.ab1
            1 TTGTACACTC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
           61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
          121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
          181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
          241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
          301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
          361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
          421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
          481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
          541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
          601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
          661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
          721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
          781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA
          841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC
          901 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG
          961 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTTATTGTCT
         1021 GGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGCTATTG AGCGCACCAG CCATCTGGTG
         1081 GCAACTCACA GTCTGGGGCA ATCCAGGCCA GCTCAGCCAA GAATCCCTGA CCTGGTGTGG
        1141 AAAAGGAAAT C
        //
        Primer: BGH_rev2
        Sequence file: RDB15448_A7Lle.seq check
        >D05018D1-1_A7Ll_BGH_rev2_E02_14_ABI24.ab1
            1 CATTCACGTC AGACGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
           61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
          121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
          181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
          241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
          301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
          361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
          421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
          481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
          541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
          601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
          661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
          721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
          781 ACGGTATCGA TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG
          841 AAGACGCGGA AGAGGCCGCA GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT
          901 GAGGGCCGAA AGGGACGTAG CAGAAGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC
          961 GAGCAGCCAT GGAAAAGGAC GTCAGCTTCC CCGACACACC ACGGAATTGT CAGTGCCCAA
         1021 CAGCCGAGCC CCTGTCCAGC AGCGGGCAGC AGCGCGATGA GTCCGCCGTG CATAGGAGGG
         1081 GGAAGCGAAG GTCCCGAAGA GCTGACTGTG TGACATGCCC ACCCAGCTGG GGATTGCCGT
        1141 TCCAGC
        //
        Primer: SV40pro_F
        Sequence file: RDB15448_A7Llf.seq check
        >D05018D1-1_A7Ll_SV40pro_F_F02_17_ABI24.ab1
            1 CCTTGTCCTT ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
           61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
          121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
          181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
          241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
          301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
          361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
          421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
          481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
          541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
          601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
          661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
          721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
          781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
          841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
          901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
          961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTT
         1021 AATGAATCGG CCAACGCGCG GGGAGAGCGT TTTGCGTATT GGCGCTCTTC GGCCTCTCGC
         1081 TCACTGACTC GCTGCGCTCG GTCGATCCGG CCTGCCGCGG AGCGGTAATC AGCCTCCACT
        1141 TCCAAAAGGG CCGGG
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl