Resource data sheet
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tFucci(CA)2.2/pCSII-EF

Lentivirus vector construct of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.

Catalog number RDB15448
Resource name tFucci(CA)2.2/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human CDT1 cDNA Human Geminin cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC212778 (DDBJ accession number)
Originator Miyawaki, Atsushi |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15448 tFucci(CA)2.2/pCSII-EF DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15448_A7Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15448_A7Lla.seq
>D05018D1-1_A7Ll_EF1a-C_F_A02_02_ABI24.ab1
    1 CATCATGCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG
   61 TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT TCTGCAGCGG
  121 CCGCGGATCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT
  181 CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG
  241 CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG
  301 TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC
  361 CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT
  421 CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC
  481 CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC
  541 CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA
  601 CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG
  661 CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG
  721 CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT
  781 GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA
  841 GGGATATCCA TCACACTGGC GGCCGCTCGA GATGGAGCAG CGCCGCGTCA CCGACTTCTT
  901 CGCGCGCCGC CGCCCCGGGC CCCCCCGCAT CGCGCCGCCC AAGCTGGCCT GCCGCACCCC
  961 CAGCCCCGCC AGGCCGCACT CGCGCCCCGG CCTCCGCTAC AGTGCCAGCG CCAAGCGCGC
 1021 CGCCGGCGGC GGCCCCCGAC GCGACAGCCA AGCAACGACC GGGCGATCGC CGCTGTCGAT
1081 GACGAAGGAT TCCAGGCTCC AGTTATCG
//
Primer: mCherry-C_F RDB15448_A7Llb.seq
>D05018D1-1_A7Ll_mCherry-C_F_B02_05_ABI24.ab1
    1 TACCAGTAGC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
   61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGATGG
  181 AGCAGCGCCG CGTCACCGAC TTCTTCGCGC GCCGCCGCCC CGGGCCCCCC CGCATCGCGC
  241 CGCCCAAGCT GGCCTGCCGC ACCCCCAGCC CCGCCAGGCC CGCACTCCGC GCCCCGGCCT
  301 CCGCTACCAG TGGCAGCCGC AAGCGCGCCC GCCCGCCCGC CGCCCCCGGA CGCGACCAGG
  361 CCAGGCCACC GGCCCGCGCG GCCGCGCGGC TGTCGGTGGA CGAGGTTTCC AGCCCCAGTA
  421 CCCCCGAGGC CCCAGACATC CCAGCCTGCC CTTCTCCGGG CCAGAAGATA AAGAAAACCG
  481 GTCGCGCCAA GCGCGGCAGC GGCGCCACCA ACTTCAGCCT GCTGAAGCAG GCCGGCGACG
  541 TGGAGGAGAA CCCCGGCCCC CGCGAATTCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
  601 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
  661 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
  721 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGAGCTGG GGCGTGCAGT
  781 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  841 AAGGCTACGT CCAGGAGCGT ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
  901 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  961 TCAAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT CAGCGACACG
 1021 TCTATATCAC CGCCCGACAG CAGAAGAACG GGCATCAAGG CCAACTTTCA AGATCCGCCA
 1081 CACATCGAGG GACGGCGGCG GTGCAGCTCG GCGGACACTA ACAAGCCAGA CCACCCCCAT
1141 TCGGGCGAAC GGGCCCCCGG AGTGCCTTGA CG
//
Primer: PRE_rev RDB15448_A7Llc.seq
>D05018D1-1_A7Ll_PRE_rev_C02_08_ABI24.ab1
    1 ACGATCAATA GCGTAAGGAG CAACATAGTT AAGAATACCA GTCAATCTTT CACAAATTTT
   61 GTAATCCAGA GGTTGATTAT CGATAAGCTT GATCCCTCGA TGTTAACTCT AGATTACAGC
  121 GCCTTTCTCC GTTTTTCTGC CACTTCCTTC CAATACTGAG AGGATGGATT TTCTTTAATC
  181 ATAAGATCAA ATGACTCCTG GGTGACTCCT CCAAGATTTT TATTTTCACT AGATTCTGGG
  241 ACAATAACCC CAGGGCTGGA AGTTGTAGAT GTTAAGTGGT CATTCCGATG TTTCCTTTTG
  301 GACAAGCCTG CGGACAGCTC ATTTTCTCTT CCAACAAGAG ATCCAGATGC AGAAGGCTGA
  361 ATCATCTTCA GAGTTCTTCT TGGGACAGAA CTATTCTTTA TATTCTCTTT GATTTCTTCT
  421 TGTTTCTGCT TCATACTGGG ATTCATCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG
  481 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
  541 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCTTGGACT GGGTGCTCAG GTAGTGGTTG
  601 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
  661 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC
  721 TGCTTGTCGG CGGTGATATA GACGTTGTCG CTGATGTAGT TGTACTCCAG CTTGTGCCCC
  781 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG
  841 TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG
  901 GTACGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
  961 TGGTCGGGGT AGCGGGCGAA GCACTGCACG CCCCAGCTCA GGTGGTCACG AGGGTGGGCC
 1021 AGGGCACGGG CAGCTTTGCC GGTGGTGCAG ATGACTTCAG GTCAGCTTGC CGTAGGTGGC
 1081 ATCGTCACGC CCTCGCGGAA CACGCTGAAC TGTGTCGATT AACGTTCGGC GTCAGCTCGG
1141 AACCGAGGAA ATGGGGCATC TA
//
Primer: CMV-Forward RDB15448_A7Lld.seq
>D05018D1-1_A7Ll_CMV-Forward_D02_11_ABI24.ab1
    1 TTGTACACTC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
  781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA
  841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC
  901 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG
  961 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTTATTGTCT
 1021 GGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGCTATTG AGCGCACCAG CCATCTGGTG
 1081 GCAACTCACA GTCTGGGGCA ATCCAGGCCA GCTCAGCCAA GAATCCCTGA CCTGGTGTGG
1141 AAAAGGAAAT C
//
Primer: BGH_rev2 RDB15448_A7Lle.seq
>D05018D1-1_A7Ll_BGH_rev2_E02_14_ABI24.ab1
    1 CATTCACGTC AGACGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
  121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
  181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
  241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
  301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
  361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
  421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
  481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
  541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
  601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
  661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
  721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
  781 ACGGTATCGA TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG
  841 AAGACGCGGA AGAGGCCGCA GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT
  901 GAGGGCCGAA AGGGACGTAG CAGAAGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC
  961 GAGCAGCCAT GGAAAAGGAC GTCAGCTTCC CCGACACACC ACGGAATTGT CAGTGCCCAA
 1021 CAGCCGAGCC CCTGTCCAGC AGCGGGCAGC AGCGCGATGA GTCCGCCGTG CATAGGAGGG
 1081 GGAAGCGAAG GTCCCGAAGA GCTGACTGTG TGACATGCCC ACCCAGCTGG GGATTGCCGT
1141 TCCAGC
//
Primer: SV40pro_F RDB15448_A7Llf.seq
>D05018D1-1_A7Ll_SV40pro_F_F02_17_ABI24.ab1
    1 CCTTGTCCTT ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
  961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTT
 1021 AATGAATCGG CCAACGCGCG GGGAGAGCGT TTTGCGTATT GGCGCTCTTC GGCCTCTCGC
 1081 TCACTGACTC GCTGCGCTCG GTCGATCCGG CCTGCCGCGG AGCGGTAATC AGCCTCCACT
1141 TCCAAAAGGG CCGGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2018.10.09

GNP_filter3_RDBDEP_html_180823.pl