Please review the QC test results indicated by icon below as well as clone information before placing your order.

tFucci(CA)2.2/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). EF-1 alpha promoter.

Sequence (full)	RDB15448zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15448_A7Llp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(tFucci(CA)2.2 5') Sequence file: RDB15448_A7Llg.seq
>D05018D1-1_A7Ll_EF1a-C_F_A02_02_ABI24.ab1     1 NNNNNNNNNT TTTNANTTTG NNNCTTGGTT NNTTCTCNAG CCTCNGACAG TGGTTCAAAG    61 TTTTTTTCTT CNNTTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT TCTGCAGCGG   121 CCGCGGATCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT   181 CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG   241 CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG   301 TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC   361 CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT   421 CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC   481 CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC   541 CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA   601 CCCCGAGGAC GGCGCCCTGA ANGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG   661 CCACTACGAC GCTGANGTCN AGANCACCTA CAAGGCCNAG AAGCCCGTGC AGCTGCCCGG   721 CGCCTACAAC GTCAACATCA AGTTGGANAT CACCTCNCAC AACGAGGANT ACACCATCGN   781 GGAACAGTAC GAANGCGCCG ANGGCCGCCA CTCCACCGGC GGNNTGGACN ANCTGTANNN   841 GGNNNATNCA TCNCACNNGN GGNCGCTCNA NATGGANCAN CGNNNCGNNN NNGANTTNNN 901 NNNGNNNNNN NNNCCNNGGN CCCCCNNNAT CNNNNNNNNN N //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(tFucci(CA)2.2 3') Sequence file: RDB15448_A7Llh.seq
>D05018D1-1_A7Ll_PRE_rev_C02_08_ABI24.ab1     1 NNNNNNNNNN NCGNNNNGAG CNNCNTAGTT AAGAATACCA GTCAATCTTT CACAAATTTT    61 GTAATCCAGA GGTTGATTAT CGATAAGCTT GATCCCTCGA TGTTAACTCT AGATTACAGC   121 GCCTTTCTCC GTTTTTCTGC CACTTCCTTC CAATACTGAG AGGATGGATT TTCTTTAATC   181 ATAAGATCAA ATGACTCCTG GGTGACTCCT CCAAGATTTT TATTTTCACT AGATTCTGGG   241 ACAATAACCC CAGGGCTGGA AGTTGTAGAT GTTAAGTGGT CATTCCGATG TTTCCTTTTG   301 GACAAGCCTG CGGACAGCTC ATTTTCTCTT CCAACAAGAG ATCCAGATGC AGAAGGCTGA   361 ATCATCTTCA GAGTTCTTCT TGGGACAGAA CTATTCTTTA TATTCTCTTT GATTTCTTCT   421 TGTTTCTGCT TCATACTGGG ATTCATCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG   481 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG   541 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCTTGGACT GGGTGCTCAG GTAGTGGTTG   601 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC   661 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC   721 TGCTTGTCGG CGGTGATATA GACGTTGTCG CTGATGTAGT TGTACTCCAG CTTGTGCNNC   781 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG   841 TCGCCCTCNA ACTTCACCTC GGCGCGGGTC TTGTAGTTGN CGNCNTCCTT GAAGAANANG   901 GNACGCTNCT NGNNGTANCC NTCNGGNATG GNNGANTTGA ANAANNCGNG CTGCTNNNNG 961 NGGNCGGGGN ANCGGGCGAA NCACTGNACG CCNNNNCNNN NNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid(tFucci(CA)2.2 mid) Sequence file: RDB15448_A7Lli.seq
>D05018D1-1_A7Ll_mCherry-C_F_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNG CCCGGCGCCN ACNACGTCNA CATCAAGTTG GACATCACCT    61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA   121 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGATGG   181 AGCAGCGCCG CGTCACCGAC TTCTTCGCGC GCCGCCGCCC CGGGCCCCCC CGCATCGCGC   241 CGCCCAAGCT GGCCTGCCGC ACCCCCAGCC CCGCCAGGCC CGCACTCCGC GCCCCGGCCT   301 CCGCTACCAG TGGCAGCCGC AAGCGCGCCC GCCCGCCCGC CGCCCCCGGA CGCGACCAGG   361 CCAGGCCACC GGCCCGCGCG GCCGCGCGGC TGTCGGTGGA CGAGGTTTCC AGCCCCAGTA   421 CCCCCGAGGC CCCAGACATC CCAGCCTGCC CTTCTCCGGG CCAGAAGATA AAGAAAACCG   481 GTCGCGCCAA GCGCGGCAGC GGCGCCACCA ACTTCAGCCT GCTGAAGCAG GCCGGCGACG   541 TGGAGGAGAA CCCCGGCCCC CGCGAATTCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG   601 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT   661 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA   721 CCGGCAAGCT GCCCGTGCCC TGNCCCACCC TCGTGACCAC CCTGAGCTGG GGCGTGCAGT   781 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCNAGTCC GCCATGCCCG   841 AANGCTACGT CCAGGANCGT ANCATCTTCT TCNAGGANGA NGGCAACTAC NAGACCNGCG   901 CCNANGNGAA GTTCGAGGGC GANNCCCNGG NGAACCGCAT CNNGCTGNNN NNNATCGNNT   961 TNNANGNANN NNGGNNNCNT NCTGGGGCAN ANNCTNNNNT ANNACTACNT CANCGANNNN 1021 NNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB15448_A7Llj.seq
>D05018D1-1_A7Ll_CMV-Forward_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNTGNN NCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC   121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA   181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG   241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC   301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG   361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG   421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT   481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC   541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC   601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA   661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT   721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA   781 TCTTCAGACC TGGANGANGA GATATGANGG ANNATTGGAG AAGTGAATTA TATAAATATA   841 AAGTAGTAAA AATTGNNCCN TTANGAGTAG CACCNNCCNA GGCNAAGAGA AGANNNGNGC   901 AGANAGAAAA AAGANCAGNG GGAATAGGAN CTTTGTNCCT NGGNNTCNTG GGNNCNNCNN 961 NAAGCNCTAN NGGNNNNNCN NNNNNNNNGN NNNNNGNACN NNNNNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cTTP_CTS,RRE Sequence file: RDB15448_A7Llk.seq
>15448_15448_B2JdF_cPPT+CTS-E_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNTTTTNN NNNNTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT    61 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT   121 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC   181 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA   241 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT   301 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT   361 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC   421 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT   481 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT   541 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAACC   601 CCAAATCCCC AGGAGCTGTT GATCCTTTAG GTATCTTTCC ACAGCCAGGA TTCTTGCCTG   661 GAGCTGCTTG ATGCCCCAGA CTGTGAGTTG CAACAGATGC TGTTGCGCCT CAATAGCCCT   721 CAGCAAATTG TTCTGCTGCT GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG   781 CGTCATTGAC GCTGCGCCCA TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGANCNAAGC   841 TCCTATTCCC ACTGCTCTTT TTTCTCTCTG CACCACTCTT CTCTTTGCCT TNGNGGGTGC   901 TACTCCNNAT GGTNCNATTT TTACNACTTT ATATTNATAN NATTCACNTN CTNNNNTTGT   961 CCCNCATATC TCCNNCNNCN ANGTCTGAAN ATCANNNNNC CTNNCTGNGN NGNGNNNNNN 1021 NNNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid(tFucci(CA)2.2 mid) Sequence file: RDB15448_A7Lll.seq
>15448_15448_B2JdF_mRFP1-inner1_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TCCTCCCTGC NGGNNGGCGA GTTCATCTAC    61 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC   121 ATGGGCNNNN AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG   181 ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC   241 TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC   301 ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC   361 CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGGGATATC CATCACACTG GCGGCCGCTC   421 GAGATGGAGC AGCGCCGCGT CACCGACTTC TTCGCGCGCC GCCGCCCCGG GCCCCCCCGC   481 ATCGCGCCGC CCAAGCTGGC CTGCCGCACC CCCAGCCCCG CCAGGCCCGC ACTCCGCGCC   541 CCGGCCTCCG CTACCAGTGG CAGCCGCAAG CGCGCCCGCC CGCCCGCCGC CCCCGGACGC   601 GACCAGGCCA GGCCACCGGC CCGCGCGGCC GCGCGGCTGT CGGTGGACGA GGTTTCCAGC   661 CCCAGTACCC CCGAGGCCCC AGACATCCCA GCCTGCCCTT CTCCGGGCCA GAAGANAAAG   721 AAAACCGGTC GCGCCAAGCG CGGCAGCGGC GCCACCAACT TCAGCCTGCT GAAGCAGGCC   781 GGCGACGTGG AGGAGAANCC CGGCCCCCGC GAATTCATGG TGAGCAANGN CGAGGANCTG   841 TTCACCNGGG GNNGTGCNCA TCCNGGTCGA GCTGGNCGGC GANGTNAACG GCCACAAGTT   901 CAGCGTGNNN GGCGANGGCG NNGGCGANGC CACCTACGGC ANGCTGANCC NNAANTTCAT 961 CNNCACCNCN GGNANNCNGC CCGNGCNNNN NNNNNN //
Primer: EGFP-N_rev (Pr0156) Region: insert mid(tFucci(CA)2.2 mid) Sequence file: RDB15448_A7Llm.seq
>15448_15448_B2JdF_EGFP-N_rev_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNGNNNNN NCGANGGCGN NGCCNCCTAC GGCAAGCTGA    61 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA   121 CCCTGAGCTG GGGCGTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT   181 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG TACCATCTTC TTCAAGGACG   241 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA   301 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT   361 ACAACTACAT CAGCGACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG   421 CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC   481 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA   541 CCCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT   601 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC   661 ACTGGCGGCC GCTCGAGATG AATCCCAGTA TGAAGCAGAA ACAAGAAGAA ATCAAAGAGA   721 ATATAAAGAA TAGTTCTGTC CCAAGAAGAA CTCTGAAGAT GANTCAGCCT TCTGCATCTG   781 GATCTCTTGT TGGAAGAGAA AATGAGCTGT CCGCANGCTT GTCCAAAANG AAACATCNNA   841 ATGACCACTT ANCATCTACA ACTTCCNGCC CTGGGGTTAT TGTCCCAGAA NTCTAGTGAA   901 AATNAAAAAT CTNNNANNAN TCANCNNNGA NTCATTTGAT NTTATGANNN AANAAAATNN 961 NNNCNTCNCN GTANNNGNAN GNNNNGNCNN ANANNNNNNN NNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,3'LTR Sequence file: RDB15448_A7Lln.seq
>D05018D1-1_A7Ll_BGH_rev2_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG GGGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC   601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG   661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA   721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCNTGTTTTT CTAGGTCTCG   781 ACGGTATCGA TGCGGGGNNG CGGCCCNAAG GGAGATCCGA NTCGTCTGAN GGCGAANGCG   841 AAGANGCGGA ANANGNCGCA NANCCNGCAG CANGNCGCGG GAANGNANGT CCGCTNGATT   901 NAGGGCCNNA NNGNACNTAN CANANNNNGT CCCGCGCANA NNNNNNNTGG NNNCACNNGN 961 GANCAGNCNN NNNAANGNNN NNCNGCTTNC CCNNNNNNNN NN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro,Zeo,SV40 pA Sequence file: RDB15448_A7Llo.seq
>D05018D1-1_A7Ll_SV40pro_F_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAN NNGTGAGGAN GNTTTTTTGG AGGCCTAGGC TTTTGCNAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCNA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGANC TCTAGCTAGA GCTTGGCGTA   841 NTCATGGTCA TAGCTGTTTC CTGTGTGAAN NTGNTATCCG CTCACAATTC CNCACNACAT   901 ACGAGCNNGA ANCATNAAGT GTAAAGNNNG GGGNGNCTAN NGAGTGANCT AACNCNCATT 961 NATTGCGTNN CGCNNNCNGN CCGNNTNNCN NNNNGNNAAN CNNNCNNGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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