RDB15982 - Achilles/pRSETB

Achilles/pRSETB (#RDB15982)

Yellow fluorescent protein clone Achilles.

Drawn by SnapGene® software

Sequence (full)	RDB15982hts01.seq
Publication	Yoshioka-Kobayashi, K., Nature 580 (7801): 119-123 (2020). [link to RRC of NBRP]
Test sheet	RDB15982_A9Ctp1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	Yellow fluorescent protein clone Achilles. Regarding the comparative characterization of Achilles versus Venus, please refer Extended Data Fig.1 of Yoshioka-Kobayashi, K. et al., Nature (2020). https://doi.org/10.1038/s41586-019-1882-z.
Vector backbone	pRSET B (Plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \| Niino, Yusuke \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Yoshioka-Kobayashi, K. et al. (2020) Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Yoshioka-Kobayashi, K. et al. (2020) Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Yoshioka-Kobayashi, K. et al. (2020) Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15982	Achilles/pRSETB	DNA solution

How to cite this biological resource

Materials & Methods section:

The Achilles/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15982).

Reference section:

Yoshioka-Kobayashi, K., Matsumiya, M., Niino, Y., Isomura, A., Kori, H., Miyawaki, A., Kageyama, R., Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123 (2020). PMID 31915376. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Yoshioka-Kobayashi, K., Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123 (2020). PMID 31915376. [link to RRC of NBRP]
reference	Diaz-Cuadros, M., In vitro characterization of the human segmentation clock. Nature 580 (7801): 113-118 (2020). PMID 31915384.

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Other resources in our bank

External Database
Aequorea victoria GFP

Reference sequence