Please review the QC test results indicated by icon below as well as clone information before placing your order.

Achilles/pRSETB

Yellow fluorescent protein clone Achilles.

Sequence (full)	RDB15982zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Yoshioka-Kobayashi, K. et al. (2020) Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Yoshioka-Kobayashi, K. et al. (2020) Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15982_A8Fip1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261) Region: 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15982_A8Fic.seq
>D05723A1_A8Fi_2_T7 20bp_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TTGNTNACTT TAAGAAGGAG NNATACATAT GCGGGGTTCT    61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT   121 CTGTACGACG ATGACGATAA GGATCCGATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG   181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGCGC   241 GGCGAGGGCG AGGGCGATGC CACCATCGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC   301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGGCCGTC   361 TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA   421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC   481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CTCCGGCTTC   541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTTCAACAG CCACAACGTC   601 TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC   661 ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC   721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGANCTACC AGTCCTTCCT GAGCAAAGAN   781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CNGGATCACT   841 CTCGGCATGG NNGANCTGTA CNAGTAAGAN NTCNAAGCTN GATNCGGCNG CTAACAAANC   901 CCGAAAGGAA GCTNANTTNG CTGCNGCCAC NGCNNNNCNN TNNNNANNAT NNNCCCNTNN 961 GGGGNCNTCT NANNGNNNNN NNNGGNNTTT TTNNNNNNAN NNNNNNNNN //
Primer: T7 terminator (Pr0155) Region: insert 3' Sequence file: RDB15982_A8Fid.seq
>D05723A1_A8Fi_2_T7 Terminator_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CCGGNNNNNN CTTCGAATTC TTACTTGTAC    61 AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA   121 TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG AAGGACTGGT AGCTCAGGTA GTGGTTGTCG   181 GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC   241 ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC   301 TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGAAGTTGT ACTCCAGCTT GTGCCCCAGG   361 ATGTTGCCGT CCTCCTTGAA GCCGGAGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG   421 CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG   481 CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG   541 TCGGGGTAGC GGGCGAAGAC GGCCAGGCCG TAGCCCAGGG TGGTCACGAG GGTGGGCCAG   601 GGCACGGGCA GCTTGCCGGT GGTGCAGATC AGCTTCAGGG TCAGCTTGCC GATGGTGGCA   661 TCGCCCTCGC CCTCGCCGCG CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG   721 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCANCAT CGGATCCTTA   781 TCGTCATCGT CGTACAGATC CCGACCCATT TGCTGTCCAC CAGTCATGCT ANCCATACCA   841 NGATGANGAT GATGATGANA ACCCCGCATA TGTATATCTN CTTCTTAAAG TTAAACAAAA 901 TTATTTCTAG NNGGGAAACC GTTGTGNTCT NCCTATAGTG AGTNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl