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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Achilles/pRSETB

Yellow fluorescent protein clone Achilles.

Catalog number RDB15982
Resource name Achilles/pRSETB
Clone info. Yellow fluorescent protein clone Achilles. Regarding the comparative characterization of Achilles versus Venus, please refer Extended Data Fig.1 of Yoshioka-Kobayashi, K. et al., Nature (2020). https://doi.org/10.1038/s41586-019-1882-z.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi | Niino, Yusuke |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT shall keep confidential information related to the MATERIAL until the Depositor publishes research results relating to the MATERIAL.
2. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
3. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
5. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
6. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Material Transfer Agreement (MTA) [Word]
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Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15982 Achilles/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Yoshioka-Kobayashi, K., Coupling delay controls synchronized oscillation in the segmentation clock. Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print] PMID 31915376.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15982_A8Fip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB15982_A8Fia.seq check
>D05723A1_A8Fi_2_T7 20bp_E01_05_ABI08.ab1
    1 GATAAACGTC TAGAATAATT TTGTTTACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT
   61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT
  121 CTGTACGACG ATGACGATAA GGATCCGATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
  181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGCGC
  241 GGCGAGGGCG AGGGCGATGC CACCATCGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC
  301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGGCCGTC
  361 TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
  421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
  481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CTCCGGCTTC
  541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTTCAACAG CCACAACGTC
  601 TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC
  661 ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
  721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCTTCCT GAGCAAAGAC
  781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
  841 CTCGGCATGG ACGAGCTGTA CAAGTAAGAA TTCGAAGCTT GATCCGGCTG CTAACAAAGC
  901 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCCTTG
  961 GGGGCCCTCT AAACGGTCTT GAGGGGTTTT TTTGCTGAAA GGAGGACTAT ATCCGGGATC
 1021 TTGGCGTAAT TAGCGAAGAG GGCCCGCACG GATCGGCCCT TTCCCTACCA GTTGCGCAGC
1081 TGAATGACGA ATTGGGGAAC GCGCCCTGGT TAGCCGGC
//
Primer: T7_terminator
Sequence file: RDB15982_A8Fib.seq check
>D05723A1_A8Fi_2_T7 Terminator_G01_07_ABI08.ab1
    1 AGACGGCTTT CTTTCGGGCT TTGTTAGCAG CCGGATCAAG CTTCGAATTC TTACTTGTAC
   61 AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA
  121 TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG AAGGACTGGT AGCTCAGGTA GTGGTTGTCG
  181 GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC
  241 ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC
  301 TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGAAGTTGT ACTCCAGCTT GTGCCCCAGG
  361 ATGTTGCCGT CCTCCTTGAA GCCGGAGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG
  421 CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG
  481 CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG
  541 TCGGGGTAGC GGGCGAAGAC GGCCAGGCCG TAGCCCAGGG TGGTCACGAG GGTGGGCCAG
  601 GGCACGGGCA GCTTGCCGGT GGTGCAGATC AGCTTCAGGG TCAGCTTGCC GATGGTGGCA
  661 TCGCCCTCGC CCTCGCCGCG CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG
  721 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT CGGATCCTTA
  781 TCGTCATCGT CGTACAGATC CCGACCCATT TGCTGTCCAC CAGTCATGCT AGCCATACCA
  841 TGATGATGAT GATGATGAGA ACCCCGCATA TGTATATCTC CTTCTTAAAG TTAAACAAAA
  901 TTATTTCTAG AGGGGAAACC GTTGTGGTCT CCCTATAGTG AGTCGGTATT AATTTCGCGG
  961 GATCGAGATC CTGCATTAAT GAATCGGGCC AACGCGCGGG GGAGAGGCGG GTTTGCGTAA
 1021 TTGGGGCGCT CTTTCGGCTT TCCTCGCTCA CTGACTCGGC TTGCGCCTCG GGTCGTTCGG
 1081 GCTGCGGCGA GCCGGTATCC AGGCTTTCAC CTCAAAAGGC CGGTTAAATA TAACCGGTTA
1141 TTAATATTGC A
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yoshioka-Kobayashi, K., Coupling delay controls synchronized oscillation in the segmentation clock. Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print] PMID 31915376.
reference Diaz-Cuadros, M., In vitro characterization of the human segmentation clock. Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1885-9. [Epub ahead of print] PMID 31915384.

2020.01.20

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