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Achilles/pRSETB (#RDB15982)

Yellow fluorescent protein clone Achilles.


Drawn by SnapGene® software
Sequence (full) RDB15982hts01.seq
Publication Yoshioka-Kobayashi, K., Nature 580 (7801): 119-123 (2020). [link to RRC of NBRP]
Test sheet RDB15982_A9Ctp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Yellow fluorescent protein clone Achilles. Regarding the comparative characterization of Achilles versus Venus, please refer Extended Data Fig.1 of Yoshioka-Kobayashi, K. et al., Nature (2020). https://doi.org/10.1038/s41586-019-1882-z.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Niino, Yusuke |

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Yoshioka-Kobayashi, K. et al., Nature 580 (7801): 119-123 (2020).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Yoshioka-Kobayashi, K. et al., Nature 580 (7801): 119-123 (2020).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15982 Achilles/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The Achilles/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15982).

Reference section:

Yoshioka-Kobayashi, K., Matsumiya, M., Niino, Y., Isomura, A., Kori, H., Miyawaki, A., Kageyama, R., Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123 (2020). PMID 31915376. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yoshioka-Kobayashi, K., Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123 (2020). PMID 31915376. [link to RRC of NBRP]
reference Diaz-Cuadros, M., In vitro characterization of the human segmentation clock. Nature 580 (7801): 113-118 (2020). PMID 31915384.