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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)5/pCS2

New color variant of Fucci: Fucci(CA)5.

Catalog number RDB18221
Resource name tFucci(CA)5/pCS2
Clone info. New color variant of Fucci: Fucci(CA)5.
Comment The accession numbers in the DDBJ/EMBL/GenBank database is LC334438.
Vector backbone pCS2 (plasmid)
Size of vector backbone 6.2 kb
Selectable markers Ampicillin
Gene/insert name Montipora monasteriata RFP cDNA Ricordea sp. GFP cDNA human CDT1 cDNA human Geminin cDNA
Reference of insert sequence LC334438 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |
Other clones in our bank

External Database
Montipora monasteriata RFP
    Ricordea sp. GFP
      human CDT1 human Geminin

        Reference sequence
          
         
        Sequence (full) RDB18221zzt01.seq compiled by us

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
        5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
        5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18221 tFucci(CA)5/pCS2 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18221_B0Fbp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pCMV-IE94_F
        Region: CMV IE94 pro, SP6 pro, insert 5'
        Sequence file: RDB18221_B0Fba.seq check
        >D07168A4_B0Fb_3_pCMV-IE94_F_D01_04_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN GNCTATATAA GCAATGCTCG TTTAGGGAAC
           61 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
          121 CTACTTGTTC TTTTTGCAGG ATCCGCCACC ATGGAGAACG TCAGGAGAAA GACAGGCATT
          181 CAGACGGAAA TGAAAACGAA GCTCCACATG GATGGTATGG TGAATGGACA CAGCTTTGAG
          241 ATCAAAGGGG AGGGGAAGGG AAGCCCGTAC GAGGGGGTGC AGACAATGAA GCTGAAGGTT
          301 ACTAAGGGCG CCCCACTTCC CTTCTCTATA GACATTCTTT TGCCTCAGTG CATGTATGGC
          361 AGCAAACCTT TCATTAAATA TCCTGAGAAC ATCCCCGATT ATTTGAAACT CTCATTTCCA
          421 GAGGGTATCA CTTGGGAGAG GACAATGACA TTCGAGGACG GCGCAGTATG CGATGTATCT
          481 AATGATTCCA GACTGGTCGG CAATTGCTTT ATCTACACTG TGAAGTTTCA GGGAGTCAAT
          541 TTTCCACTTG ATGGCCCCGT TATGCAAAAG AAGACCCGAG GCTGGGAACC CTCCACTGAA
          601 GTGCTGTACG AATGTGATGG GTGGATGCGC GGGTTGGTGG ACATAGCTCT GAAACTGGAA
          661 AACGGTGGAC ATTATATGTG TAATTTTAAG ACCACCTACA AATCCAAGAA AGGTCTTGAG
          721 GTGCCACCTT ACCATTTCGT GGATCATAAA CTCGACCTGC TCAGTCATAA CACCGACGGA
          781 NCCACCTTTG AGGAGTTCGA ACAANGTGAA ATCGCTCACG CGCACTTGAN TAAACTGGCC
          841 GGANATNCAT CACACNGGCG GCCGCTCNNN ATGGANCANC GCCGCGNCAC CGANTTCTTC
          901 GCGCGCCGCC GCCCCGGGCC CCCCCCGNAT CGCGCCGCCN AANCNGGNNN NNCGCACCCC
        961 CNANCCCCNC CAGGNCNGCN NNNNNNGNCC CGGNCNNNCN NNNNNNNNN
        //
        Primer: pGCAP_R
        Region: T7 pro, insert 3'
        Sequence file: RDB18221_B0Fbb.seq check
        >D07168A4_B0Fb_3_pGCAP_R_B03_06_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNCG NNAATACGAC TCACTATAGG GCTGCAGAAT
           61 CTAGATTACA GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA
          121 TTTTCTTTAA TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA
          181 CTAGATTCTG GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA
          241 TGTTTCCTTT TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT
          301 GCAGAAGGCT GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT
          361 TTGATTTCTT CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT
          421 GGATATCCCT TATTCAGGTT CTTTTCAGGC AGCAGGCTGT ACCTGGCCAC AGCCCCTGCG
          481 TAGAGCTTGA CAGGGCGTCC TTCGGGCTTC TCCAGGATCT CGATCCGGTG GTCCACGAAG
          541 TGGTAATCTG GCATCTTCTC CACCTTATTT TTGGGAATAT AAGAAGTGTT GAAATCGACT
          601 CTGAGGTGAC CACCATCCTT CAACAGCAGG GCCATATTCA CGTCGCCCTT CAGCACTCCA
          661 TCGTTTTTAT CGTCCTTGTA CATCACCTCG GTGGAAGGTT CCCATTTGAC TGTTTCCTTT
          721 TGCATGACTG GGCCGTGGGG GGGGAANTTT CCGTTGAATT GTATTTTATA ATGGAAGCAG
          781 TCCCCTTGAA CGCTGATATC GGANTCAACG GTTGANACGC CGCCNNCTTC ATAGGTCATT
          841 GTTCTTTTCC ATGTGTATCC CCCGCCNCNG NAANGNNTGT TTGAAGAANT CTGGTATGTT
          901 NNCGGGGNAC TNNGNNAACG CCCTATTGNC GNACTGAAAN GNTGGGNNCA GNNNATCNAA
          961 NCTGNANNTC ANNGNNNTTN CNNNCNNNNN NNNNCNNGNN AANNGNNNGA NNNNNNNNNN
        //
        Primer: Azalea5B_F
        Region: insert mid
        Sequence file: RDB18221_B0Fbc.seq check
        >D07168A4_B0Fb_3_Azalea5B_F_C03_09_ABI24.ab1
            1 NNNNNNNNNN NNNNNTNCNA ATCNNAGAAA GGNCTTGAGG TGCCNCCTTA CCNTTTCGTG
           61 GATCATAAAC TCGACCTGCT CAGTCATAAC ACCGACGGAG CCACCTTTGA GGAGTTCGAA
          121 CAAGGTGAAA TCGCTCACGC GCACTTGAGT AAACTGGCCG GATATCCATC ACACTGGCGG
          181 CCGCTCGAGA TGGAGCAGCG CCGCGTCACC GACTTCTTCG CGCGCCGCCG CCCCGGGCCC
          241 CCCCGCATCG CGCCGCCCAA GCTGGCCTGC CGCACCCCCA GCCCCGCCAG GCCCGCACTC
          301 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC
          361 GGACGCGACC AGGCCAGGCC ACCGGCCCGC GCGGCCGCGC GGCTGTCGGT GGACGAGGTT
          421 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG
          481 ATAAAGAAAA CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG
          541 CAGGCCGGCG ACGTGGAGGA GAACCCCGGC CCCCGCGAAT TCATGAGTAA ACTCCAGAAA
          601 GGTGTGGAGA AAGAGATGAA GATTAAGCTC ACTATGAACT GCACCGTGAA CGAACGGAAC
          661 TTTGTGATCA CAGGGCAGGG GGCTGGGGAA CCTTATGATG GAACTCAGAC ACTTTACCTG
          721 ACAGTGGANG GCGGAAAGAC ACTGAACTTC AGCTTCGATG TACTGACCCC AGCCTTTCAG
          781 TACGGCAATA GGGCGTTTAC CAAGTACCCC GGTAACATAC CAGACTTCTT CAAACAGACC
          841 TTCAGTGNCG GGGGGANACA CATGGAAAAG AACNATGACC TATGAAGACG GCGGCGTCTC
          901 AACCGTNGAG NNCGATATCA GCGTTCAAGG GGGNNNGCTN NCNTNNTAAA ATNCNANTCN
          961 NNGNAAANNT NCCCCCCCNN NNNGNCCNAG NCATGNNAAN NNAAACNNNN NANNNGGNNN
        1021 NCNNNNNNNN NN
        //
        Primer: tFucci_f1
        Region: insert mid
        Sequence file: RDB18221_B0Fbd.seq check
        >D07168A4_B0Fb_3_tFucci_f1_D03_12_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNTCTCNNN NNCNNAAGNN AAAGAAAAAC CGGTCGCGCC
           61 NNAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG CAGGCCGGCG ACGTGGAGGA
          121 GAACCCCGGC CCCCGCGAAT TCATGAGTAA ACTCCAGAAA GGTGTGGAGA AAGAGATGAA
          181 GATTAAGCTC ACTATGAACT GCACCGTGAA CGAACGGAAC TTTGTGATCA CAGGGCAGGG
          241 GGCTGGGGAA CCTTATGATG GAACTCAGAC ACTTTACCTG ACAGTGGAGG GCGGAAAGAC
          301 ACTGAACTTC AGCTTCGATG TACTGACCCC AGCCTTTCAG TACGGCAATA GGGCGTTTAC
          361 CAAGTACCCC GGTAACATAC CAGACTTCTT CAAACAGACC TTCAGTGGCG GGGGATACAC
          421 ATGGAAAAGA ACAATGACCT ATGAAGACGG CGGCGTCTCA ACCGTTGAGT CCGATATCAG
          481 CGTTCAAGGG GACTGCTTCC ATTATAAAAT ACAATTCAAC GGAAAGTTCC CCCCCCACGG
          541 CCCAGTCATG CAAAAGGAAA CAGTCAAATG GGAACCTTCC ACCGAGGTGA TGTACAAGGA
          601 CGATAAAAAC GATGGAGTGC TGAAGGGCGA CGTGAATATG GCCCTGCTGT TGAAGGATGG
          661 TGGTCACCTC AGAGTCGATT TCAACACTTC TTATATTCCC AAAAATAAGG TGGAGAAGAT
          721 GCCAGATTAC NACTTCGTGG ACCACCGGAT CGAGATCCTG GAGAAGCCCG AAGGACGCCC
          781 TGTCAAGCTC TACGCAGGGG CTGTGGCCAG GTACAGCCNG CTGCCTGAAA AGAANCTGAA
          841 TANNGGATAT CCATCACACT GGCGGCCGCT CGAGATGANT CCCAGTATGA AGCANAANCA
        901 AGAANNAAAN TCAAAGANAN TATAAANNAA TAGTTNCTGN CCNNNNNNNN NN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl