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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(CA)5/pCS2

New color variant of Fucci: Fucci(CA)5.

Catalog number RDB18221
Resource name tFucci(CA)5/pCS2
Clone info. New color variant of Fucci: Fucci(CA)5.
The accession numbers in the DDBJ/EMBL/GenBank database is LC334438.
Vector backbone pCS2 (plasmid)
Size of vector backbone 6.2 kb
Selectable markers Ampicillin
Gene/insert name Montipora monasteriata RFP cDNA Ricordea sp. GFP cDNA human CDT1 cDNA human Geminin cDNA
Reference of nucleotide sequence LC334438 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18221 tFucci(CA)5/pCS2 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18221_B0Fbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F
Region: CMV IE94 pro, SP6 pro, insert 5'
Sequence file: RDB18221_B0Fba.seq check
>D07168A4_B0Fb_3_pCMV-IE94_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN GNCTATATAA GCAATGCTCG TTTAGGGAAC
   61 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
  121 CTACTTGTTC TTTTTGCAGG ATCCGCCACC ATGGAGAACG TCAGGAGAAA GACAGGCATT
  181 CAGACGGAAA TGAAAACGAA GCTCCACATG GATGGTATGG TGAATGGACA CAGCTTTGAG
  241 ATCAAAGGGG AGGGGAAGGG AAGCCCGTAC GAGGGGGTGC AGACAATGAA GCTGAAGGTT
  301 ACTAAGGGCG CCCCACTTCC CTTCTCTATA GACATTCTTT TGCCTCAGTG CATGTATGGC
  361 AGCAAACCTT TCATTAAATA TCCTGAGAAC ATCCCCGATT ATTTGAAACT CTCATTTCCA
  421 GAGGGTATCA CTTGGGAGAG GACAATGACA TTCGAGGACG GCGCAGTATG CGATGTATCT
  481 AATGATTCCA GACTGGTCGG CAATTGCTTT ATCTACACTG TGAAGTTTCA GGGAGTCAAT
  541 TTTCCACTTG ATGGCCCCGT TATGCAAAAG AAGACCCGAG GCTGGGAACC CTCCACTGAA
  601 GTGCTGTACG AATGTGATGG GTGGATGCGC GGGTTGGTGG ACATAGCTCT GAAACTGGAA
  661 AACGGTGGAC ATTATATGTG TAATTTTAAG ACCACCTACA AATCCAAGAA AGGTCTTGAG
  721 GTGCCACCTT ACCATTTCGT GGATCATAAA CTCGACCTGC TCAGTCATAA CACCGACGGA
  781 NCCACCTTTG AGGAGTTCGA ACAANGTGAA ATCGCTCACG CGCACTTGAN TAAACTGGCC
  841 GGANATNCAT CACACNGGCG GCCGCTCNNN ATGGANCANC GCCGCGNCAC CGANTTCTTC
  901 GCGCGCCGCC GCCCCGGGCC CCCCCCGNAT CGCGCCGCCN AANCNGGNNN NNCGCACCCC
961 CNANCCCCNC CAGGNCNGCN NNNNNNGNCC CGGNCNNNCN NNNNNNNNN
//
Primer: pGCAP_R
Region: T7 pro, insert 3'
Sequence file: RDB18221_B0Fbb.seq check
>D07168A4_B0Fb_3_pGCAP_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNCG NNAATACGAC TCACTATAGG GCTGCAGAAT
   61 CTAGATTACA GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA
  121 TTTTCTTTAA TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA
  181 CTAGATTCTG GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA
  241 TGTTTCCTTT TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT
  301 GCAGAAGGCT GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT
  361 TTGATTTCTT CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT
  421 GGATATCCCT TATTCAGGTT CTTTTCAGGC AGCAGGCTGT ACCTGGCCAC AGCCCCTGCG
  481 TAGAGCTTGA CAGGGCGTCC TTCGGGCTTC TCCAGGATCT CGATCCGGTG GTCCACGAAG
  541 TGGTAATCTG GCATCTTCTC CACCTTATTT TTGGGAATAT AAGAAGTGTT GAAATCGACT
  601 CTGAGGTGAC CACCATCCTT CAACAGCAGG GCCATATTCA CGTCGCCCTT CAGCACTCCA
  661 TCGTTTTTAT CGTCCTTGTA CATCACCTCG GTGGAAGGTT CCCATTTGAC TGTTTCCTTT
  721 TGCATGACTG GGCCGTGGGG GGGGAANTTT CCGTTGAATT GTATTTTATA ATGGAAGCAG
  781 TCCCCTTGAA CGCTGATATC GGANTCAACG GTTGANACGC CGCCNNCTTC ATAGGTCATT
  841 GTTCTTTTCC ATGTGTATCC CCCGCCNCNG NAANGNNTGT TTGAAGAANT CTGGTATGTT
  901 NNCGGGGNAC TNNGNNAACG CCCTATTGNC GNACTGAAAN GNTGGGNNCA GNNNATCNAA
  961 NCTGNANNTC ANNGNNNTTN CNNNCNNNNN NNNNCNNGNN AANNGNNNGA NNNNNNNNNN
//
Primer: Azalea5B_F
Region: insert mid
Sequence file: RDB18221_B0Fbc.seq check
>D07168A4_B0Fb_3_Azalea5B_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNCNA ATCNNAGAAA GGNCTTGAGG TGCCNCCTTA CCNTTTCGTG
   61 GATCATAAAC TCGACCTGCT CAGTCATAAC ACCGACGGAG CCACCTTTGA GGAGTTCGAA
  121 CAAGGTGAAA TCGCTCACGC GCACTTGAGT AAACTGGCCG GATATCCATC ACACTGGCGG
  181 CCGCTCGAGA TGGAGCAGCG CCGCGTCACC GACTTCTTCG CGCGCCGCCG CCCCGGGCCC
  241 CCCCGCATCG CGCCGCCCAA GCTGGCCTGC CGCACCCCCA GCCCCGCCAG GCCCGCACTC
  301 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC
  361 GGACGCGACC AGGCCAGGCC ACCGGCCCGC GCGGCCGCGC GGCTGTCGGT GGACGAGGTT
  421 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG
  481 ATAAAGAAAA CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG
  541 CAGGCCGGCG ACGTGGAGGA GAACCCCGGC CCCCGCGAAT TCATGAGTAA ACTCCAGAAA
  601 GGTGTGGAGA AAGAGATGAA GATTAAGCTC ACTATGAACT GCACCGTGAA CGAACGGAAC
  661 TTTGTGATCA CAGGGCAGGG GGCTGGGGAA CCTTATGATG GAACTCAGAC ACTTTACCTG
  721 ACAGTGGANG GCGGAAAGAC ACTGAACTTC AGCTTCGATG TACTGACCCC AGCCTTTCAG
  781 TACGGCAATA GGGCGTTTAC CAAGTACCCC GGTAACATAC CAGACTTCTT CAAACAGACC
  841 TTCAGTGNCG GGGGGANACA CATGGAAAAG AACNATGACC TATGAAGACG GCGGCGTCTC
  901 AACCGTNGAG NNCGATATCA GCGTTCAAGG GGGNNNGCTN NCNTNNTAAA ATNCNANTCN
  961 NNGNAAANNT NCCCCCCCNN NNNGNCCNAG NCATGNNAAN NNAAACNNNN NANNNGGNNN
1021 NCNNNNNNNN NN
//
Primer: tFucci_f1
Region: insert mid
Sequence file: RDB18221_B0Fbd.seq check
>D07168A4_B0Fb_3_tFucci_f1_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCTCNNN NNCNNAAGNN AAAGAAAAAC CGGTCGCGCC
   61 NNAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG CAGGCCGGCG ACGTGGAGGA
  121 GAACCCCGGC CCCCGCGAAT TCATGAGTAA ACTCCAGAAA GGTGTGGAGA AAGAGATGAA
  181 GATTAAGCTC ACTATGAACT GCACCGTGAA CGAACGGAAC TTTGTGATCA CAGGGCAGGG
  241 GGCTGGGGAA CCTTATGATG GAACTCAGAC ACTTTACCTG ACAGTGGAGG GCGGAAAGAC
  301 ACTGAACTTC AGCTTCGATG TACTGACCCC AGCCTTTCAG TACGGCAATA GGGCGTTTAC
  361 CAAGTACCCC GGTAACATAC CAGACTTCTT CAAACAGACC TTCAGTGGCG GGGGATACAC
  421 ATGGAAAAGA ACAATGACCT ATGAAGACGG CGGCGTCTCA ACCGTTGAGT CCGATATCAG
  481 CGTTCAAGGG GACTGCTTCC ATTATAAAAT ACAATTCAAC GGAAAGTTCC CCCCCCACGG
  541 CCCAGTCATG CAAAAGGAAA CAGTCAAATG GGAACCTTCC ACCGAGGTGA TGTACAAGGA
  601 CGATAAAAAC GATGGAGTGC TGAAGGGCGA CGTGAATATG GCCCTGCTGT TGAAGGATGG
  661 TGGTCACCTC AGAGTCGATT TCAACACTTC TTATATTCCC AAAAATAAGG TGGAGAAGAT
  721 GCCAGATTAC NACTTCGTGG ACCACCGGAT CGAGATCCTG GAGAAGCCCG AAGGACGCCC
  781 TGTCAAGCTC TACGCAGGGG CTGTGGCCAG GTACAGCCNG CTGCCTGAAA AGAANCTGAA
  841 TANNGGATAT CCATCACACT GGCGGCCGCT CGAGATGANT CCCAGTATGA AGCANAANCA
901 AGAANNAAAN TCAAAGANAN TATAAANNAA TAGTTNCTGN CCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

2020.12.17

GNP_filter3_RDBDEP_html_201216.pl