Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tdoxStayGold/pcDNA3

Fluorescent protein tdoxStayGold for fusion to the C-terminus of target molecule

Catalog number RDB19611
Resource name tdoxStayGold/pcDNA3
Clone info. For fusion of tdoxStayGold to the C-terminus of a target molecule (X). tdoxStayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, oxStayGold, c4, EV linker, n1, and oxStayGold. The gene for the former oxStayGold has been resynthesized with mammalian-preferred codons, and the gene for the latter oxStayGold has the jellyfish original codons. n1 and c4 are the appendages that facilitate fusions to the N- and C-termini of oxStayGold, respectively. X-tdoxStayGold is required to terminate with the original C-terminus of oxStayGold. oxStayGold is a variant of StayGold with two mutations: C174I and C208I; oxStayGold is well expressed also in the cytosolic fraction as StayGold. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank

External Database
Cytaeis uchidae GFP

            Reference sequence
              
             
            Sequence (full) RDB19611zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19611 tdoxStayGold/pcDNA3 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content 明るく、退色しにくい新規の緑色蛍光タンパク質 「StayGold」 (Japanese text)
            Featured content StayGold, highly photostable GFP (English text)
            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19611_B2Eap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert 5'((GGGGS)x3,tdoxStayGold 5')
            Sequence file: RDB19611_B2Eaa.seq check
            >D08836A7_B2Ea_2_CMV_Forward_A06_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT GNNGNNAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
               61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
              121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCGGATGG TGGAGGCGGT TCAGGCGGAG
              181 GTGGCTCTGG CGGTGGCGGA TCGATCAAGC TTATCGATAC CGTCGACCTC GAGATGGTGT
              241 CTACAGGCGA GGAGCTGTTT ACCGGCGTGG TGCCCTTCAA GTTCCAGCTG AAGGGCACCA
              301 TCAACGGCAA GAGCTTCACC GTGGAAGGCG AGGGCGAGGG CAATAGCCAC GAGGGCAGCC
              361 ACAAAGGCAA GTACGTGTGC ACCAGCGGCA AACTGCCAAT GTCTTGGGCC GCCCTGGGAA
              421 CTAGCTTCGG CTATGGCATG AAGTACTACA CCAAGTACCC CAGCGGCCTG AAGAACTGGT
              481 TCCACGAGGT GATGCCCGAG GGCTTCACCT ACGACAGACA CATCCAGTAC AAGGGCGACG
              541 GCAGCATCCA CGCCAAGCAC CAGCACTTCA TGAAGAACGG CACCTACCAC AACATCGTGG
              601 AGTTCACCGG CCAGGACTTC AAGGAGAACA GCCCCGTGCT GACCGGCGAC ATGAACGTGA
              661 GCCTGCCCAA CGAGGTGCAG CACATCCCCA GAGATGACGG CGTGGAGTGC CCAGTGACCC
              721 TGCTGTACCC TCTGCTGANC GACAAGAGCA AGTGCGTGGA GGCCTACCAG AACACCATCA
              781 TCAAGCCCCT GCACAATCAG CCAGCCCCCG ATGTGCCATA CCACTGGATC AGAAAGCAGT
              841 ACACCCAGAN CAAGGANGAN ACCGANGNNA NAGANCACAT CATCCAGANC GAGANCCTGG
              901 ANGNCCACCT GNNNTGGCNC GANCCTTCNG CTTCNGNCGN GGAATTCANN GCTGGTGGNT
              961 CTGCTNGANN NANNGNNNGN NNTNNNNCNN GGGGNNNCGN NNNNGNNTCC NNCNNCNCNN
            1021 GCNNNNNNNN NN
            //
            Primer: BGH_rev2
            Region: insert 3'(tdoxStayGold 3')
            Sequence file: RDB19611_B2Eab.seq check
            >D08836A7_B2Ea_2_BGH_rev2_D06_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GNCANNNNNN NNANGGCTGG CAACTAGNNA
               61 GGCACNNGTC GAGGCTNNNN NNNNGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC
              121 CCTCTAGATG CATGCTCGAG CGGCCGCTTA TAAGTGTGCT TCGAGAGTCT CTGATTGAAT
              181 AATATGATCA CGTTCCTCGG TATCATCTTT GCTTTGTGTG TATTGTTTAC GAATCCAGTG
              241 ATATGGGACA TCAGGTGCTG GTTGATTATG AAGAGGCTTG ATGATTGTAT TTTGGTAAGC
              301 CTCAACGCAT TTTGATTTAT CCGATAATAA AGGATAAAGC AAGGTCACTG GGCATTCTAC
              361 TCCATCATCT CTGGGTATAT GTTGAACTTC ATTCGGTAAT GAGACATTCA TATCTCCAGT
              421 TAAGACTGGA CTATTTTCTT TAAAATCCTG ACCAGTAAAT TCAACAATGT TGTGATAAGT
              481 CCCATTTTTC ATAAAGTGTT GGTGTTTTGC ATGGATACTC CCATCGCCTT TATATTGAAT
              541 ATGACGATCG TAGGTAAAGC CTTCAGGCAT TACTTCATGA AACCAGTTCT TCAGTCCACT
              601 AGGATATTTG GTATAATATT TCATTCCATA ACCAAAGGAT GTTCCAAGTG CTGCCCATGA
              661 CATCGGTAGT TTTCCACTTG TACAAACATA TTTTCCTTTA TGAGAACCTT CATGTGAATT
              721 TCCTTCACCT TCGCCTTCAA CGGTAAACGA TTTGCCATTG ATGGTTCCTT TAAGTTGAAA
              781 TTTAAATGGC ACCACCCCGG TGAACAGCTC CTCCCCTGTA CTCACCATAA GCTTCCCACC
              841 GGCGCTACCG CCGGCACTCC CTCCGGNGGA NNCGCCAGCA NANCCACCTG CAGATGTGGA
              901 NCCTCNNGCG CTNNCACCTG CNCTTNCAGA NCCCCCGGCA GANCCANCNG CGCTNCCANC
              961 AGNNGNANNN TNCTGCNCTN CCCCCAGNNN ANGNNNTTNC NNNNGNNANA NNCNNCNGNN
            1021 NGNNNCNGNN NNNNNNNN
            //
            Primer: hStayGold_F1
            Region: insert mid
            Sequence file: RDB19611_B2Eac.seq check
            >D08836A7_B2Ea_2_hStayGold_F1_B06_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG NNNNCCTGCC NNNCNAGGTG CAGCACATCC CCAGAGATGA
               61 CGGCGTGGAG TGCCCAGTGN CCCTGCTGTA CCCTCTGCTG AGCGACAAGA GCAAGTGCGT
              121 GGAGGCCTAC CAGAACACCA TCATCAAGCC CCTGCACAAT CAGCCAGCCC CCGATGTGCC
              181 ATACCACTGG ATCAGAAAGC AGTACACCCA GAGCAAGGAC GACACCGAGG AGAGAGACCA
              241 CATCATCCAG AGCGAGACCC TGGAGGCCCA CCTGCCATGG CACGAGCCTT CTGCTTCTGC
              301 CGTGGAATTC AGTGCTGGTG GTTCTGCTGG AGGAAGCGCC GGAGGTTCCG CTGGGGGAAG
              361 CGCAGGCGGC TCCGGCAGCG CTGGCGGGAG TGCCGGGGGC AGTACCTCCG CAGGAGGAAG
              421 TGCAGGGGGA AGCGCTGGTG GCAGTGCGGG GGGTTCCGCA GGCGGTAGCG GTTCCGCCGG
              481 TGGATCTGCC GGTGGAAGCA CATCTGCTGG GGGTAGTGCA GGAGGGTCCG CTGGTGGGAG
              541 CGCAGGTGGA TCTGCCGGGG GGTCTGGAAG TGCAGGTGGT AGCGCTGGAG GGTCCACATC
              601 TGCAGGTGGG TCTGCTGGCG GTTCCGCCGG AGGGAGTGCC GGCGGTAGCG CCGGTGGGAA
              661 GCTTATGGTG AGTACAGGGG AGGAGCTGTT CACCGGGGTG GTGCCATTTA AATTTCAACT
              721 TAAAGGAACC ATCAATGGCA AATCGTTTAC CGTTGAAGGC GAANGTGAAG GAAATTCACA
              781 TGAAGGTTCT CATAAAGGAA AATATGTTTG TACAAGTGGA AAACTACCGA TGTCATNNNN
              841 AGCACTTGNA ACATCCTTTG GTTATGGAAT GAAATATTAT ACCAAATATC CTAGTGNANT
              901 GAAGAACTGG TTTCATGAAG TAATGCCTGN ANNNNTTTAC CTACNATCGN CATATTCNNT
              961 ATAAAGGNNA TGGGANTATC CNTGCNAAAC NCCNNCNCTT TATGAAAAAT GGGNNNTATC
            1021 NNNNNNNNNN
            //
            Primer: EV_linker_F1
            Region: insert mid
            Sequence file: RDB19611_B2Ead.seq check
            >D08836A7_B2Ea_2_EV_linker_F1_D10_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN GNNNNNNCGC ANGTGGNNCT GCCGGGGGGT CTGGAAGTGC
               61 AGGTGGTAGC GCTGGAGGGT CCACATCTGC AGGTGGGTCT GCTGGCGGTT CCGCCGGAGG
              121 GAGTGCCGGC GGTAGCGCCG GTGGGAAGCT TATGGTGAGT ACAGGGGAGG AGCTGTTCAC
              181 CGGGGTGGTG CCATTTAAAT TTCAACTTAA AGGAACCATC AATGGCAAAT CGTTTACCGT
              241 TGAAGGCGAA GGTGAAGGAA ATTCACATGA AGGTTCTCAT AAAGGAAAAT ATGTTTGTAC
              301 AAGTGGAAAA CTACCGATGT CATGGGCAGC ACTTGGAACA TCCTTTGGTT ATGGAATGAA
              361 ATATTATACC AAATATCCTA GTGGACTGAA GAACTGGTTT CATGAAGTAA TGCCTGAAGG
              421 CTTTACCTAC GATCGTCATA TTCAATATAA AGGCGATGGG AGTATCCATG CAAAACACCA
              481 ACACTTTATG AAAAATGGGA CTTATCACAA CATTGTTGAA TTTACTGGTC AGGATTTTAA
              541 AGAAAATAGT CCAGTCTTAA CTGGAGATAT GAATGTCTCA TTACCGAATG AAGTTCAACA
              601 TATACCCAGA GATGATGGAG TAGAATGCCC AGTGACCTTG CTTTATCCTT TATTATCGGA
              661 TAAATCAAAA TGCGTTGAGG CTTACCAAAA TACAATCATC AAGCCTCTTC ATAATCAACC
              721 AGCACCTGAT GTCCCATATC ACTGGATTCG TAAACAATAC ACACAAAGCA AAGATGANAC
              781 CGAGGAACGT GATCATATTA TTCAATCNGA GNCTCTCGAA GCACACTTAT AAGCGGCCGC
              841 TCGANCATGC NTCTAGAGGG NCCTATTCTA TAGTGTCACC TAAATGCTAN AGCTCGCTGA
              901 NCAGCCTCNA CTGTGCCTTC TANTTGNCNG CCNTCTGNTG TTTGCCCNNC CCCCNNNCCT
              961 TNNNTGNNCC NNNNANNGNG CCNCNNCCNC TGNCCTTNNC NANNAAAATG NNNAANNNGC
             1021 NTCGNNNTNN NTGANTNNNN GNCATTCNNN NTNNNNGGNG NNNNNNNNNG GGNNNNNNNN
            1081 NN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR
            Sequence file: RDB19611_B2Eae.seq check
            >D08836A7_B2Ea_2_SV40pro_ori_F_E06_15_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
              121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
              181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
              241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
              301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
              361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
              421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
              481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
              541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
              601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
              661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
              721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGNNCGCTTT TCTGGATTCA TCGACTGTGG
              781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACNCGTG ATATTGCTGA
              841 AGANCTTGGC GGCGAATGGG CTGACCGCTT CCTCGNGCTT TACGGTATCG CCGCTCCCGA
              901 TTCNCAGCGC ATCGCCTTCN ATCGCCTTCT TGANNANTTC TTCTGANCGG GNNNCTGGGG
              961 TTCGAAATGA NCNANCNANC GANGCCNNAN CNNGNNNTCN CGANATTTCN NNTNCNNCNN
            1021 CNNNCNNNCN ANNAANNNNN GGGNTTNNGN NNNNNNTTTN NNGNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl